响应面法优化人工蛹虫草子实体中虫草酸微波提取工艺

为优化人工蛹虫草子实体中虫草酸的微波提取工艺,在单因素试验基础上,利用响应曲面法考察提取时间、微波功率和液固比对虫草酸提取率的影响,优化提取工艺.结果表明:人工培养蛹虫草子实体中虫草酸微波提取的优化工艺条件为:微波功率555 W,液固比35 mL/g,提取时间4min,提取2次,虫草酸提取率的理论值可达84.45％.     

提取防风多糖的工艺优化

采用超声波强化和微波辅助提取2种方法提取防风多糖,并与传统热水浸提法在多糖的提取率上进行比较。防风多糖超声提取的最佳工艺条件为超声功率1 000 W、提取时间25 min、液固体积质量比为25,防风多糖微波提取的最佳工艺条件为微波功率560 W、液固体积质量比为30、提取时间10 min,在最佳提取工艺下,2种方法的提取率分别为6.103%和7.639%。与传统热水浸提法相比,超声法和微波法提取防风多糖具有迅速、节能、高效、提取率高等诸多优点。     

以松毛虫为基质培养的冬虫夏草菌丝体中多糖的提取研究

研究了不同方法对冬虫夏草发酵菌丝体（以枯叶蛾科昆虫马尾松毛虫为基质发酵所得）中多糖提取的影响。结果表明,采用微波辅助水提法所得多糖的产率最高,影响提取的关键因素为液料比、微波提取时间、微波功率;采用Box-Behnken设计及响应面分析法对这3个因素进行优化,并通过回归拟合,建立了预测虫草多糖提取的多项式模型Y=6.87+0.058A+0.085B+0.075C+0.032AB+0.046AC+0.069BC-0.16A²-0.37B²-0.11C²。经响应面最优化分析,获得冬虫夏草发酵菌丝体中多糖的最优提取工艺参数为：液料比（mL/g）6.3:1、微波功率520W、微波提取时间326s,此工艺提取验证后的提取率达到6.76%。     

微波辅助提取补阳还五汤多糖的研究

采用单因素实验和正交实验法,进行了微波辐射萃取补阳还五汤多糖的最佳工艺条件研究。探讨了不同微波辐射时间、微波功率及料液比等条件对多糖提取率的影响。结果表明,在微波辐射时间20min,微波功率325W,料液比为1：7,补阳还五汤中多糖的提取率为7．75％。微波辅助萃取大大缩短了提取时间,节省了溶剂,提高了收率。     

微波协同酶法提取平菇柄多糖的工艺研究

为优化微波协同酶法提取平菇柄多糖的工艺条件,在单因素试验的基础上,选择提取时间、微波功率以及液料比为自变量,多糖得率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖得率的影响。利用响应面分析方法,模拟得到二次多项式回归方程的预测模型,并确定平菇柄多糖提取工艺的最佳条件为:微波功率420 W,提取时间8 min,液料比55:1,在此条件下,最大得率达到6.05%。     

微波辅助提取灰树花多糖工艺研究

采用提取时间、微波功率、液料比的单因素试验和正交试验法优化微波辅助提取灰树花多糖条件.结果表明,以净多糖得率为指标,影响微波辅助提取灰树花多糖的主次因素为:提取时间＞微波功率＞液料比,并且提取时间和微波功率的影响达到了极显著水平.灰树花多糖最佳提取工艺条件为:提取时间为10 min,微波功率为80%(全功率为800 W),液料比为25∶1.创立了一种用苯酚-硫酸法测定多糖时排除蛋白质干扰的方法.     

超声微波协同萃取(UMAE)番茄中番茄红素的研究

研究了超声微波协同萃取番茄中的番茄红素。以乙酸乙酯为萃取溶剂并以不同的提取时间、微波功率和液固比作为参数进行实验,并进行了工艺优化。在提取时间367s、微波功率98W和液固比10．6：1的条件下实际提取率可达到97．4％。实验结果表明：UMAE法是一种能够快速有效的提取番茄红素的方法。     

微波辅助提取石榴皮多糖工艺优化及其免疫调节作用研究

优化石榴皮多糖的微波辅助提取工艺并初步探究石榴皮多糖的免疫调节作用。在单因素试验的基础上,以液料比、微波提取时间、微波功率为自变量,石榴皮多糖得率为响应值,利用响应曲面分析方法,确定石榴皮多糖提取工艺的最佳条件为:液料比44∶1 m L/g,微波提取时间10 min,微波功率450 W,在该条件下石榴皮多糖的得率为10.48%。通过MTT法、中性红法、NO释放量测定和黏附试验初步表明石榴皮多糖对RAW264.7巨噬细胞具有一定的免疫调节作用。     

微波法从蛹虫草中提取虫草多糖的技术研究

以蛹虫草(Cordycepsmilitaris)为原料,采用微波法对其虫草多糖的提取工艺进行了探讨。通过对其微波功率、提取时间和料水质量比进行正交优化,得到一个最佳提取工艺条件为:微波功率700 W、提取时间2.0 min、料水质量比1:60。通过正交优化的多糖得率为3.78%,比常规热水浸提法高出0.79个百分点。故此,利用微波法从蛹虫草中提取虫草多糖具有得率高、能耗小、操作简单等优点。     

Two Extraction Methods of Lentinan Polysaccharide

采用正交试验比较研究热水浸提法和微波提取法提取香菇多糖.热水浸提法的最佳工艺为:提取温度70℃、提取时间4h、料液比1∶15,提取率3.243 ％;微波提取法的最佳工艺为:微波功率560W、微波处理时间60 s、料液比1∶10,提取率4.771％.微波提取法效率高、时间短,是理想的香菇多糖提取方法.     

微波法提取鼠尾藻多糖的工艺研究

研究采用了微波法提取鼠尾藻多糖,考查了微波辐照时间、微波功率、料液比、超声预处理时间四个因素的影响,并采用正交实验法确定微波提取的最佳工艺。结果表明微波法提取多糖产率为6.5%,其优化工艺条件为:微波辐照时间8 min,微波功率540 W,料液比1∶30,超声时间3 min。经红外光谱得知鼠尾藻多糖的主要组成单糖为吡喃糖。微波法提取鼠尾藻多糖可行,工艺简单,产率令人满意。     

Optimization of extraction process of Glycyrrhiza glabra polysaccharides by response surface methodology

Experimental design was used to investigate the effect of three parameters (extraction time, extraction number and ratio of water to raw material) on polysaccharides yields. The ranges of the factors investigated were 3.5–4.5 h for extraction time (X₁), 4–6 for extraction number (X₂), and 25–35 for ratio of water to raw material (X₃). The statistical analysis of the experiment indicated that extraction time and ratio of water to raw material had significant effect on Glycyrrhiza glabra polysaccharides yields. The central composite design showed that polynomial regression models were in good agreement with the experimental results with the coefficients of determination of 0.924 for Glycyrrhiza glabra polysaccharides yield. The optimal condition for Glycyrrhiza glabra polysaccharides yield within the experimental range of the variables studied was at 4.3 h, 6, and 35. At this condition, the predicted yield of polysaccharides extracted was 3.6%.     

灰树花多糖的提取及抗氧化活性

采用单因素和正交试验法对热水浸提、超声波辅助和微波辅助提取灰树花多糖的工艺进行研究,分别获得了3种方法的最佳条件,并发现微波辅助法最佳,其最优条件为:微波功率800 W、微波时间3 min,浸提2h,多糖得率为13.05％,比热水浸提法提高了51.22％.利用提取的灰树花多糖进行抗氧化性研究,发现灰树花多糖对O2-·和·OH都具有一定的清除作用,且多糖浓度与其对O2-·的清除作用存在量效关系,但不如·OH明显.     

响应面法优化微波辅助提取枇杷叶中黄酮类化合物

为充分利用枇杷叶,研究微波辅助乙醇浸提法从枇杷叶中提取黄酮类化合物的工艺。首先研究了影响提取效果的因素:不同体积分数的乙醇溶液、料液比、时间及微波功率。在单因素试验基础上利用响应面分析法,确定了各因素与总黄酮得率之间的关系,并以总黄酮得率为响应值做响应面和等高线图。研究表明,枇杷叶中黄酮类化合物提取最佳工艺为乙醇体积分数50%,料液比1∶30,提取时间3 min,微波功率500 W,最佳条件下总黄酮得率为2.04%,与理论推导值2.07%相比,误差为1.4%,黄酮类化合物响应方程的回归分析表明此方法合理可行。本研究对枇杷叶总黄酮提取优化的研究为进一步探讨枇杷叶的深度开发提供了理论参考依据,并为枇杷叶总黄酮的生物活性研究提供材料。     

Ultrasonic-assisted extraction of polysaccharides from Hohenbuehelia serotina by response surface methodology

An ultrasonic-assisted procedure for the extraction of polysaccharides from the fruiting body of Hohenbuehelia serotina was investigated using response surface methodology. The effects of four factors on the yield of polysaccharides were studied. The optimized conditions were extraction temperature 94°C, extraction time 3.0h, ratio of water to raw material 110:1 and ultrasonic power 480W. Under these conditions, the experimental yield of polysaccharides was 17.45±0.18%, which was well matched with the predictive yield of 17.54%. The molecular weight of polysaccharide was ranged from 1.19×10(3) to 1.55×10(4)Da. The polysaccharides were composed of ribose, arabinose, mannose, glucose and galactose in a ratio of 0.65:0.69:9.35:14.24:5.47. Then, the structural features of untreated materials, hot water extraction residue and ultrasonic-assisted extraction residue were compared by SEM. Results indicated that ultrasonic-assisted extraction technology could be an effective and advisable technique.     

Optimization of ultrasonic-assisted extraction process of Poria cocos polysaccharides by response surface methodology

Polysaccharides production from Poria cocos was carried out using aqueous NaOH with the assistance of ultrasonic. Experimental design was used to investigate the effect of three parameters (extraction time, extraction concentration of NaOH, and ratio of aqueous NaOH to raw material) on polysaccharides yields. The ranges of the factors investigated were 1–3 min for extraction time (X₁), 0.5–1.0 mol/L for extraction concentration of NaOH (X₂), and 30–50 for ratio of aqueous NaOH to raw material (X₃). The statistical analysis of the experiment indicated that extraction concentration of NaOH had significant effect on P. cocos polysaccharides yields. The central composite design showed that polynomial regression models were in good agreement with the experimental results with the coefficients of determination of 0.9935 for P. cocos polysaccharides yield. The optimal condition for P. cocos polysaccharides yield within the experimental range of the variables studied was at 2.44 min, 0.789 mol/L, and 53.0. At this condition, the predicted yield of polysaccharides extracted was 82.3%.     

微波提取香水莲花总黄酮的工艺研究

该文研究了微波法提取香水莲花总黄酮的工艺。采用比色法测定黄酮的含量。利用单因素实验和正交实验考察了乙醇浓度、微波功率、微波时间、料液比对黄酮得率的影响,确定了最佳工艺为:用70%的乙醇溶液,微波功率250W,提取时间40 min,液料比25∶1,提取的总黄酮得率最高。