十八里长峡自然保护区国家级保护植物研究

对湖北省竹溪县十八里长峡省级自然保护区内的国家重点、珍稀濒危野生植物、珍贵树种、重点保护野生药材植物多样性进行了研究。确认十八里长峡自然保护区内有共有国家级保护植物83种。其中属国家重点保护植物的有28种(I级6种,II级22种),占湖北省50种的56%;国家级珍稀濒危植物的有53种(一级2种,二级15种,三级36种),国家珍稀濒危植物54种(1级2种,2级15种,3级37种),占湖北省总数67种的80.59%;国家珍贵树种的有21种(1级5种,2级16种),占湖北省总数32种的65.62%。上述三类保护植物共有67种,占湖北省国家级保护植物三类总数88种的76.13%;属国家重点保护药用植物的有21种(Ⅱ级4种,Ⅲ级17种),占全国重点保护野生药用植物总数58种的36.2%。     

中国国家重点保护野生植物的就地保护现状

就地保护即建立自然保护区是保护珍稀濒危野生植物的最佳手段。本研究以文献资料为基础, 分析了截至2008年我国建立的国家级自然保护区对已正式颁布的《国家重点保护野生植物名录(第一批)》中所列物种的就地保护状况。在全国尺度上, 国家级自然保护区共保护国家重点保护野生植物237种(含变种), 占总数的80.07%。其中I级保护植物56种。在省级尺度上, 云南、广西、四川、贵州、湖南5省区国家级自然保护区内分布的国家重点保护野生植物数量最多。通过分析各省区未受到国家级自然保护区保护的重点保护野生植物分布状况, 提出了国家重点保护野生植物就地保护的热点地区。     

北京松山国家级自然保护区脊椎动物区系初报

北京松山自然保护区野生脊椎动物相当丰富,初步调查有脊椎动物53科183种及亚种,其中兽类15科29种,鸟类26科119种及6亚种,爬行类5科15种,两栖类2科2种,鱼类2科12种。有国家一级重点保护动物3种,国家二级重点保护动物8种,北京市重点保护动物14种,市级一般保护野生动物50种。保护区内动物区系的复杂性与松山自然保护区地貌和小气候多样性有关。     

基于随机森林模型的国家重点保护陆生脊椎动物物种优先保护区的识别

准确可靠地识别国家重点保护陆生脊椎动物物种的优先保护区,是生物多样性保护的热点问题之一。采用随机森林(random forests)模型,基于12个环境变量,对中国263种国家重点保护陆生脊椎动物建模,并预测各个物种在背景点的适生概率,迭加计算得到国家重点保护陆生脊椎动物物种的生境适宜性指数。此外,基于对生境适宜性指数的空间自相关分析,识别和确定国家重点保护陆生脊椎动物物种优先保护区,并对优先保护区目前的被保护情况进行分析。结果表明,国家重点保护陆生脊椎动物物种的优先保护区的面积为103.16万km~2,约占我国国土面积的10.90%。优先保护区主要分布在我国的西部地区,包括西南地区的秦岭-大巴山山区、云南省与印度及缅甸的交界地区、武陵山山区、喜马拉雅山-横断山脉山区、阿尔泰山脉山区、天山山脉山区、昆仑山山脉山区;东北的大、小兴安岭、东北-华南沿海地区及长江中下游地区有少量分布。优先保护区中被保护的面积为50.40万km~2,占优先保护区总面积的48.86%,保护率偏低,未被充分保护。利用系统聚类分析,将未被保护的优先保护区划分成3种优先保护顺序,以期为相关部门的决策提供科学依据,更好地保护生物多样性。     

陕西青木川国家级自然保护区脊椎动物 物种多样性现状和保护成效

野生动物资源本底是自然保护区开展生物多样性有效管理的基础。为了掌握脊椎动物资源现状和评估保护成效,在2017和2018年,利用样线法和红外相机陷阱法等方法,对陕西青木川国家级自然保护区脊椎动物资源进行了实地调查,并采用专家打分法对该保护区保护成效进行赋值评估。结果表明：（1）青木川自然保护区现已记录野生脊椎动物342种,隶属5纲32目91科233属,其中,国家I级、II级重点保护野生动物分别为10种和41种;与2002年保护区建立初期相比,增加了4目9科46属83种,其中,鸟类物种数增加最多,达55种,两栖类仅增加1种。（2）青木川镇游客总量从2009年的5万余人次/年,发展到2017年的162万人次/年,保护区外围的旅游开发对该保护区脊椎动物及栖息地均有一定的潜在影响。（3）过去30年内土地利用模式变化明显,2001年未建立保护区时,该区域农业用地面积约占总面积的33%,建立保护区后至2018年,农业用地仅占保护区总面积的1%,使得区域内脊椎动物的栖息地面积显著增加。（4）保护区的保护成效显著,主要干扰因素发生变化,表现为保护区脊椎动物物种总数明显增加,栖息地适宜性和区域完整性显著增强,主要干扰因素类型已由早期的农林牧渔活动转变为现在的外围旅游开发。     

七姊妹山自然保护区野生保护植物优先保护定量研究

在野外调查和查阅文献资料的基础上,研究了七姊妹山自然保护区国家重点保护野生植物的分布情况,并运用濒危系数、遗传价值系数和物种价值系数对其优先保护顺序进行了定量分析.结果表明:(1)七姊妹山自然保护区共有国家重点保护野生植物28种,其中Ⅰ级7种,Ⅱ级21种,分别占湖北省分布的60.9%、87.5%和55.30%;(2)采用优先保护评价体系对28种国家重点保护植物进行排序,其结果与《国家重点保护野生植物名录(第一批)》结果有所不同,属一级保护的有银杏和伯乐树2种,占总数的7.14%;属二级保护的有桢楠、连香树、光叶珙桐、红豆杉、珙桐、黄杉、花榈木、篦子三尖杉、毛红椿、巴山榧树、水青树、红豆树、闽楠、南方红豆杉、莼菜、红椿和川黄檗17种,占总数的60.71%;属三级保护的有喜树、香果树、鹅掌楸、榉树、厚朴和凹叶厚朴6种,占总数的21.43%;属四级保护的有金荞麦、樟树和野大豆3种,占总数的10.71%;(3)该评价体系对28种植物的评价与分级结果更真实地反映了它们在该区域受威胁状态和优先保护顺序,对该区野生重点保护植物的优先保护工作具有一定指导意义.     

北京山地植物多样性优先保护地区评价

为促进北京市植物多样性的保护及自然保护区的合理建设与布局,以2001～2006年间在北京市13个自然保护区、森林公园或者风景名胜区内实地调查的1168个样地数据为基础,根据在北京市分布的25种珍稀濒危植物种,以及丰富度高的群落类型、面积较大的天然森林群落类型和具有重要生态功能的森林群落的评定结果,采用等级赋值的方法,利用地理信息系统软件从植物物种和植物群落方面综合评定了北京市山地植物多样性的优先保护地区.评定出综合优先保护地区总面积为184474.64hm2,约占市国土面积的11.25%,其中一级优先保护地区总面积24850.50hm2,二级优先保护地区总面积78606 60hm2,三级优先保护地区总面积81017.54hm2.优先保护地区主要分布于怀柔区、延庆县、房山区和密云县等远郊区县,结合北京市自然保护区分布现状,提出了北京市自然保护区建设优化方案.     

我国哺乳动物就地保护状况评估

自然保护区被公认为是生物多样性就地保护最有效的方式之一。基于全国自然保护区基础调查,通过分析我国自然保护区内野生哺乳动物种群数量的分布情况,在全国尺度上对自然保护区内591种哺乳动物的就地保护状况进行了评估。根据评价指标体系,将我国哺乳动物就地保护水平划分为"有效保护""较好保护""一般保护""较少保护""未受保护""保护状况不明""未予评价"等7个等级。结果表明,除未予评价的35种外,其余参评的556种物种中,共有534种在自然保护区内得到一定程度的保护,占总数的90.36%。其中有效保护、较好保护、一般保护、较少保护的物种分别有145、60、100种和229种,分别占总数的24.53%、10.15%、16.92%和38.75%。此外,有22种物种保护状况不明。针对不同保护体系中的哺乳动物的就地保护状况进行评价,研究共涉及国家重点保护野生动物140种和列入中国物种红色名录的427种,其中分别有109种和409种在保护区内得到不同程度的保护。总体来说,虽然有90.36%的哺乳动物在保护区内得到不同程度的保护,但保护状况良好(包括有效保护和较好保护)的比例明显较低,保护状况仍然不容乐观,保护力度有待加强;对于保护状况不明的物种,有必要对保护区开展更加全面、细致的科学考察以进一步确定其保护现状;对于确未受到自然保护区就地保护的物种,需要通过完善保护区网络,将其纳入自然保护体系之中。     

中国自然保护区建设与发展

生物多样性保护,在当今世界倍受重视,是全球性环境保护行动计划的重要组成部分。生物多样性是指地球上所有生物——动物、植物和微生物及其所构成的综合体,包括生物物种多样性、遗传基因多样性和生态系统多样性3个部分。在保护生物多样性的途径方面,除了易地保护和离体遗传种质保护外,更主要的是就地保护,即建立自然保护区。中国的生物多样性损失较为严重,大约有200种植物已经灭绝,估计另有5000种植物在近期处于濒危状态,占中国高等植物总数的20%。另有近400种动物(脊椎动物)处于濒危之中,占中国脊椎动物总数的7.7%。因此,中国的生物多样性…     

浙江省珍稀植物资源及其在自然保护区的分布

浙江省国家重点保护野生珍稀濒危植物51种,占全国珍稀濒危总数419种的12．2％,本文报道了浙江珍稀濒危植物资源的基本概况和自然保护区的分布。并对其保护对策进行了探讨。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.