降钙素基因相关肽对血管内膜损伤后内皮细胞功能恢复的 …

在球囊剥脱大鼠主动脉内膜造成的内皮损伤模型上,用ＣＧＲＰ治疗后观察血管内皮合成释放ｖＷＦ含量及ｔ－ＰＡ活性的变化。结果表明ＣＧＲＰ能明显降低ｖＷＦ含量ｔ－ＰＡ活性。提示ＣＧＲＰ能三轻内皮损伤反应,在促内皮细胞增殖的同时对内皮某些功能恢复亦有一定的作用。     

蝮蛇抗栓酶对不稳定性心绞痛患者血中部分凝血及纤溶指标的影响

测定３８例不稳定性心绞痛（ＵＡＰ）患者血中纤维蛋白原（Ｆｇ）含量、组织型纤溶酶原激活物（ｔ－ＰＡ）活性、血管性假血友病因子（ｖＷＦ）活性和血小板聚集率水平（ＰＡｇＴ）并与２１名正常人对比。患者中１８例用蝮蛇抗栓酶治疗,２０例行常规治疗。结果显示：ＵＡＰ患者血中ＰＡＩ－１、ｖＷＦ以及ＰＡｇＴ水平明显增高,ｔ－ＰＡ水平明显降低;蝮蛇抗栓酶组治疗后血中Ｆｇ、ＰＡＩ－１、ＰＡｇＴ水平均明显下降,使ｔ－ＰＡ水平明显升高;常规治疗组治疗前后各项指标无明显变化。认为蝮蛇抗栓酶对ＵＡＰ有可靠疗效     

炎症介质对离体大鼠肠系膜动脉降钙素基因相关肽释放的影响

本工作目的是在离体大鼠肠系膜动脉床灌流模型上,观察几种常见炎症介质：前列腺素Ｅ２（ＰＧＥ２）、缓激肽（ＢＫ）、组胺（ＨＩＳ）、血小板活化因子（ＰＡＦ）及５－羟色胺（５－ＨＴ）对血管周围感觉神经介质ＣＧＲＰ释放的直接影响。结果显示：ＰＧＥ２（１－１００μｍｏｌ／Ｌ）和ＢＫ（５－１０μｍｏｌ／Ｌ）能引起大鼠肠系膜动脉床时间和浓度依赖性地释放ＣＧＲＰ。ＨＩＳ,ＰＡＦ和５－ＨＴ则未见明显作用。结果提示,ＰＧＥ２与ＢＫ可能是引起血管周围感觉神经兴奋和ＣＧＲＰ释放的主要炎症介质。     

15—Mt—PGF2α对CCL4所致大鼠离体肝细胞损伤的保护作用

采用大鼠离体肝细胞原代培养２４ｈ,并利用四氯化碳ＣＣｌ４造成急性肝细胞损伤模型,检定１５－甲基－前列腺素Ｆ２α（１５－Ｍｔ－ＰＧＦ２α）对肝细胞损伤的影响。结果表明：（１）１５－Ｍｔ－ＰＧＦ２α可显著降低中毒肝细胞脂质过氧化物水平,抑制肝细胞脂质过氧化,并降低谷丙转氨酶（ＧＰＴ）和谷草转氨酶（ＧＯＴ）水平,稳定脂质膜。（２）显著促进中毒肝细胞ＲＮＡ和ＤＮＡ的合成。（３）超微结构证实１５－Ｍｔ－ＰＧＦ２α能减轻ＣＣｌ４对肝细胞脂质膜,染色质,线粒体,内质网和核蛋白体的损害。     

低氧对肺动脉内皮细胞PDGF—B链释放及平滑肌细胞生长的影响

应用蛋白ｄｏｔｂｌｏｔ技术检测了低氧内皮细胞条件培养液（ＨＥＣＣＭ）和常氧内皮细胞条件培养液（ＮＥＣＣＭ）内ＰＤＧＦ相对含量,并利用［３Ｈ］－ＴｄＲ掺入法和流式细胞术观察了ＨＥＣＣＭ和ＮＥＣＣＭ及加入特异ＰＤＧＦ抗体对肺动脉平滑肌细胞（ＰＡＳＭＣ）生长的影响。结果表明,ＨＥＣＣＭ中的ＰＤＧＦ含量明显高于ＮＥＣＣＭ;ＨＥＣＣＭ能明显增强ＰＡＳＭＣ内ＤＮＡ合成,促进ＰＡＳＭＣ从Ｇｏ／Ｇ１期进入Ｓ期;当预先加入ＰＤＧＦ－Ｂ链抗体时,则会明显地抑制ＨＥＣＣＭ对ＰＡＳＭＣ的ＤＮＡ合成,阻止ＰＡＳＭＣ从Ｇｏ／Ｇ１期进入Ｓ期。结果提示,低氧时ＰＡＳＭＣ增殖与肺动脉内皮细胞分泌释放ＰＤＧＦ增加有关     

不同应激损伤所致血管内皮细胞中vWF的变化

目的：通过检测血管内皮细胞（ＶＥＣ）中ｖＷＦ的变化,评价ＶＥＣ的损伤。方法：利用免疫组化技术对体外培养的猪肺动脉内皮细胞（ＰＡＥＣ）和大鼠主动脉内皮细胞（ＡＥＣ）中ｖＷＦ进行免疫荧光标记,通过流式细胞仪对ｖＷＦ的细胞阳性率和荧光强度进行定性、定量分析,观察低氧和低温所引起的ｖＷＦ变化。结果：正常猪ＰＡＥＣ和大鼠ＡＥＣ的ｖＷＦ阳性率在８０％左右,阳性程序较高。但缺氧或冷冻损伤可使ＰＡＥＣ和ＡＥＣ的ｖ     

人组织型纤溶酶原激活剂（t－PA）突变体FrGGI的构建、表达及特性分析

为了获得半衰期延长,特异活性提高及具有ＰＡＬ－１抗性的新型ｔ－ＰＡ溶栓剂,利用基因重组及定位突变技术构建了ｔ－ＰＡ的Ｋ１、Ｋ２区糖基化位点消除,ＰＡＩ－１结合位点缺失,Ｆ与Ｅ区连接序列Ｈｉｓ４４～Ｓｅｒ５０置换为纤粘蛋白Ⅰ型Ｆ区间连接序列ＧｌｕＳｅｒＬｙｓＰｒｏＧｌｕＡｌａＧｌｕＧｌｕ的ｔ－ＰＡ组合突变体ＦｒＧＧＩ,并在中国仓鼠卵巢细胞中获得了高效表达。对表达产物的生物学特性分析表明,ＦｒＧＧＩ在大鼠血浆中的半衰期延长了１５倍,并获得了ＰＡＩ－１抗性,是一株很有希望的新型溶栓剂候选株。     

几种扩血管多肽对bFGF促血管平滑肌细胞增殖作用的影响

目的和方法：研究肾上腺髓质素（Ａｄｍ）、降钙素基因相关肽（ＣＧＲＰ）及Ｃ－型心房利太（ＣＮＰ）对碱性成纤维细胞生长因子（ｂＦＧＦ）促血管平滑细胞（ＶＳＭＣ）增殖作用的影响及其机制。结果：孵育２４ｈ后,ｂＦＧＦ刺激ＶＳＭＣ增殖较对照组增加２．１倍（Ｐ〈０．０１）,细胞内蛋白磷酸化程度增加１．４倍（Ｐ〈０．０１）,ＰＫＣ及ＭＡＰＫ活性分别增加１．５和２．５倍（Ｐ〈０．０１０;Ａｄｍ．ＣＧＲＰｔ ＣＮＰ     

人组织型纤溶酶原激活剂（t—PA）突变体FrGGI的构建,表达及特性…

为了获得半衰期延长,特异活性提高及具有ＰＡＩ－１抗性的新型ｔ－ＰＡ溶性剂,利用基因重组及定位突变技术构建了ｔ－ＰＡ的Ｋ１、Ｋ２区糖基化位点消除,ＰＡＩ－１结合位点缺失,Ｆ与Ｅ区连接序列Ｈｉｓ４４￣Ｓｅｒ５０置换为纤粘蛋白Ｉ型Ｆ区间连接序列Ｇｌｕ Ｓｅｒ Ｌｙｓ Ｐｒｏ Ｇｌｕ Ａｌａ Ｇｌｕ Ｇｌｕ的ｔ－ＰＡ组合突变体ＦｒＧＧＩ,并在中国仓鼠卵巢细胞中获得了高效表达,对表达产物的生物学特性分析表明     

降钙素基因相关肽拮抗内皮素的致心律失常作用

本工作在麻醉大鼠冠状动脉口注射内皮素１（ＥＴ１）９００ｐｍｏｌ／ｋｇ能引起室性早搏（ＰＶＣ）、室速（ＶＴ）、室颤（ＶＦ）等严重心律失常,心律失常评分（ＡＳ）为５．６±１．０;冠状动脉口单独注射降钙素基因相关肽（ＣＧＲＰ）３００－１２００ｐｍｏｌ／ｋｇ仅引起血压一过性下降,此后逐渐恢复,无心律失常发生,心律失常评分为０。用ＣＧＲＰ３００ｐｍｏｌ／ｋｇ预处理后再注射ＥＴ１９００ｐｍｏｌ／ｋｇ,心律失常发生率减少,严重程度降低,ＡＳ为１．６±１．６。ＣＧＲＰ１２００ｐｍｏｌ／ｋｇ＋ＥＴ１组心律失常评分显著低于ＥＴ１对照组（Ｐ＜０．０１）。本实验结果表明,ＣＧＲＰ的抗心律失常作用很可能有部分是通过拮抗内皮素的致心律失常作用来实现的。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.