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金针菇菌丝体深层培养工艺研究 总被引:3,自引:1,他引:2
在1.8L气升式发酵反应器中,研究了金针菇菌丝体深层培养工艺条件及其特性。结果表明:金针菇菌丝体深层培养可采用玉米、黄豆和糖蜜等廉价原料作主要碳氮源;最适需氧量为1.0—2.0×10-7mol O2/ml·min·atm,培养24—72h内,菌丝球生长遵循立方根规律·即x1/3,=0.786+0.027t。在最佳发酵条件下,摇瓶培养4天,茵丝干重达24.4mg/ml在反应器培养4天,菌丝干重为25.7mg/ml。 相似文献
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金针菇在淀粉废水中发酵的营养条件研究 总被引:1,自引:0,他引:1
用摇瓶试验对金针菇菌丝体在淀粉废水中培养的营养条件进行了研究。结果表明,利用淀粉废水进行金针菇液体发酵的最适营养条件为:经液化处理的淀粉废水,加KH2PO40.25 g/100 mL,MgSO4·7H2O0.05g/100mL,VB1150μg/L,VB250μg/L,pH5.40。测定了该营养条件下菌体的生长曲线及发酵过程中培养基残糖的变化。发酵周期为7d,发酵终点生物量达2.08 g/100 mL,COD去除率为70.8%。 相似文献
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尖镰孢胞外青霉素V酰化酶的产生 总被引:1,自引:0,他引:1
由腐殖土中分离出一株产胞外青霉素V酰化酶的尖镰孢(Fusariumoxysporum),编号FP941。研究了该菌在液体培养基中产胞外青霉素V酰化酶的条件。在以10%麦麸为碳源的培养基中,添加氮源能促进酶的形成。无机氮源优于有机氮源。(NH4)2HPO4的促进效果最佳,草酸铵次之,用量均为1%。为提高产酶量,培养基中添加诱导物是必要的、苯氧乙酸的诱导效果最佳,用量为0.1%,其次是青霉素V,用量为0.3%。最适培养条件为:培养 相似文献
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本文对4种羊肚菌在固体发酵条件下的菌丝生物量和降解淀粉作用进行了研究,结果表明:羊肚菌(Morchella esculenta)、尖顶羊肚菌(M conica)、黑脉羊肚菌(M angusticeps)和皱柄羊肚菌(M. crassipes)在玉米粉培养基或马铃薯粉培养基上进行固体发酵时,菌丝生物量之间无显著差异;但a-淀粉酶活力、淀粉降解率差异显著。4种羊肚菌中,尖顶羊肚菌的降解淀粉能力最强。在培养基中添加Ca2+和氮源以及将发酵时间从15天延长到25天均能显著提高羊肚菌的菌丝生物量、a-淀粉酶活力和淀粉降解率。在添加10%黄豆粉、0.1%Ca(Cl)225℃发酵25天的玉米粉和马铃薯粉的发酵产物中,尖顶羊肚菌对玉米淀粉的降解率可达到74.2%,对马铃薯淀粉的降解率可达到79.8%。 相似文献
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植物细胞离析酶的制备和应用 总被引:2,自引:0,他引:2
用 Aspergillus sp.A-19菌经固体发酵研制成一种新的植物细胞离析酶(SeparatasezA—P)。其离析单细胞的酶活力平均为70 767u/g,有效作用的pH在3.0—7.0,温度为20—45℃。发酵培养基配方是麸皮:桔皮粉:(NH4)2SO4(w/w)为100:100:O.63,料水比为1 :2.0,培养适宜条件为25℃、60小时。 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献