草莓组织培养研究初报

取草莓茎尖,进行离体培养,分化出再生植株、完正植株放于低温(5—10℃)处,壮苗锻炼、获得同步苗、移栽成活率80％以上,通过电镜检测茎尖苗无病毒粒体、无毒草莓所内小区试验获得增产13.9～22.48％的明显效果。     

栓皮栎茎段离体培养的研究

以4～6个月的实生苗茎段为外植体,研究了离体条件下培养基及激素对栓皮栎器官发生和植株再生的影响。结果表明:初代培养选用低盐培养基WPM、BTM和GD,附加0.2mg·L-16-BA,丛生芽较多,茎粗壮,生长势健壮,均好于高盐培养基MS。继代培养时,以BTM为基本培养基,添加0.2～0.6mg·L-16-BA有利于茎芽增殖和生长,当6-BA浓度为0.8～1.0mg·L-1时,不利于茎芽伸长。以WPM为基本培养基,附加NAA0.1mg·L-1和IBA0.25mg·L-1时,生根率高,根系发育好。     

芦笋皮层组织培养植株再生

报道了芦笋皮层组织培养再生植株的方法。表明：皮层在ＭＳ＋１ｍｇ／Ｌ２,４—Ｄ＋２ｍｇ／Ｌ６—ＢＡ培养基上形成无色或淡绿色疏松型愈伤组织,而ＭＳ＋２ｍｇ／Ｌ６—ＢＡ＋０．１ｍｇ／ＬＩＡＡ培养基则对芽的分化有利;试管苗的生很壮苗以无激素的ＭＳ为最佳培养基。此外,本试验还研究了不同碳源、不同激素组合对芦笋皮层离体培养再生植株的影响。     

山葵未成熟胚离体培养和植株再生

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)日本品种岛根 3号。2　材料类别　开花 2 0ｄ后的荚果中剥出的未成熟种子 (种皮 未成熟胚 )。3　培养条件　基本培养基为自行设计的“贵山”(代号ＧＳ)配方[1 ] 。 ( 1 )诱导愈伤组织培养基为 :ＧＳ 6 ＢＡ 0 .1ｍｇ·Ｌ- 1 (单位下同 ) 2 ,4 Ｄ 1 ;( 2 )芽分化培养基为 :ＧＳ 6 ＢＡ 0 .5 ＺＴ 0 .5 ＩＢＡ0 .0 1 ;( 3)芽增殖培养基为 :ＧＳ 6 ＢＡ 0 .4;( 4 )生根培养基为 :ＧＳ ＩＢＡ 0 .1 ＮＡＡ 0 .1。以上培养基均含 3%蔗糖 [( 1 )含 2 % ]和 0 .6%琼脂粉 ,ｐＨ5 .8～ 5…     

槭叶茑萝的离体培养与植株再生

1植物名称槭叶茑萝(Quamoclit sloteri House),别名葵叶茑萝。2材料类别带节茎段。3培养条件以MS为基本培养基。(1)芽诱导培养基:MS KT 2.0mg·L-1(单位下同) IBA 0.1;(2)     

西南金丝桃茎段的离体培养和植株再生

1　植物名称　西南金丝桃 (Hypericumhenryi)。2　材料类别　当年生茎段。3　培养条件　以MS为基本培养基。 ( 1 )腋芽诱导培养基 :MS + 6 BA 1 .0mg·L- 1 (单位下同 ) ;( 2 )芽增殖培养基 :MS + 6 BA 1 .0 +KT 1 .0 ;( 3)生根培养基 :1 /2MS。上述培养基均添加 3%蔗糖、0 .65 %琼脂 ,pH 5 .8～ 6.0。培养温度为 2 4℃ ,每日光照1 2～ 1 4h ,光照度 1 5 0 0lx。4　生长与分化情况4.1　芽的诱导　野外采集的茎段用常规法灭菌后 ,切成 2cm长的小段 (带 1个节 ) ,基部向下直插于 ( 1 )号培养基上。培养 5d后 ,腋芽开始萌动生长 ;1 5d后…     

高效诱导冬瓜再生植株的研究

将台湾冬瓜的种子接种于pH值为7.2的1/2MS培养基上预培养,5d左右种子即可萌发,萌发率为100%,幼苗生长正常。切取预培养15-20d的无菌幼苗的茎尖和带腋芽的茎段接种于MS 1mg/LNAA 4mg/L6-BA培养基上,10d左右在茎尖和茎段（带腋芽）切口处长出愈伤组织,30d左右在愈伤组织处分化出丛生芽,丛生芽的诱导频率接近95%,繁殖系数25.6。将小芽切下转入不加任何生长调节剂MS培养基上,培养几天后芽逐渐长大,并在芽的基部长出白色根系。选取生长健壮的试管苗经过炼苗后移栽到大田中,生长良好。     

钻天杨的组织培养与植株再生

1植物名称钻天杨(Populus nigra L.var.italica)。2材料类别无菌苗叶片。3培养条件(1)芽分化培养基:MS TDZ 0.1 mg·L~(-1)(单位下同) NAA 0.01;(2)生根培养基:1/2MS IBA 0.5;上述培养基中均加入0.7%琼脂和2%蔗糖,pH 5.8。培养基在121℃条件下灭菌20 min。培养温度为(25±2)℃,每天光照和暗     

梳唇石斛成熟胚的离体培养和植株再生

1植物名称梳唇石斛(Dendrobium strongylanthum Rchb.f.). 2材料类别成熟种子. 3培养条件以MS和1/2MS为基本培养基.(1)种子萌发培养基:MS NAA 0.2 mg·L-1(单位下同) 6-BA 0.4 马铃薯汁200g·L-1;(2)原球茎诱导增殖培养基:1/2MS 6-BA 0.5;(3)原球茎分化培养基:1/2MS 6-BA 0.5 NAA 0.5 椰子汁200g·L-1 ;(4)壮苗及生根培养基:1/2MS NAA 0.5 香蕉泥100g·L-1 .上述培养基均附加20 g·L-1 蔗糖、8 g·L-1琼脂,pH 5.5～5.8.培养温度为(24±2)℃,光照时间12 h·d-1,光照度1 000～2000 lx.     

Regeneration of Cucumis sativus var. sativus and C. sativus var. hardwickii,C. melo,and C. metuliferus from explants through somatic embryogenesis and organogenesis

Regeneration in six inbred lines or F₁ hybrids of Cucumis sativus was achieved on Murashige & Skoog's medium containing various concentrations of 2,4-D/BA, NAA/BA, NAA/Z or NAA/K. The range of regeneration frequency for cotyledon, leaf and petiole explants was 0–38, 0–75 and 14–96%, respectively, after 6–8 weeks in culture. Only one subculture of calli to growth regulator-free medium was required for regeneration. Preincubation of explants in the dark for 2–3 weeks was essential to achieve optimal regeneration. Highest frequency of plantlet formation occurred with petiole explants incubated on NAA/BA (5.0/2.5 M), NAA/Z (5.0/5.0 M) or 2,4-D/BA (5.0/5.0 M). Approximately 80% of these plantlets survived after transplanting to greenhouse soil, and they flowered and set fruit. The F₁ hybrid, Endeavor, gave the highest regeneration frequency of 91% on 2,4-D/BA at 5.0/5.0 M. Formation of somatic embryos was observed on 2,4-D/BA, while organogenesis and embryogenesis both were evident on NAA/BA and NAA/Z. Cotyledonary explants yielded the lowest frequency (ca. 7%) of plantlet formation in this study. Plantlets of C. sativus var. hardwickii and an F₁ hybrid of C. sativus x C. s. var hardwickii were regenerated on NAA/Z and NAA/K at frequencies of 15–65%, predominantly by the formation of somatic embryos. Shoots were obtained from cotyledon and leaf explants of C. metuliferus on IAA/BA (7.5/5.0 M) and from leaf and petiole explants of C. melo on NAA/BA (5.0/2.5 M), but plantlets were recovered only in C. melo.Abbreviations BA benzyladenine - 2,4-D 2,4-dichlorophenoxy-acetic acid - IAA indoleacetic acid - K kinetin - MS Murashige & Skoog's medium - NAA naphthaleneacetic acid - Z zeatindihydroside     

云南的天然秃杉林及其群落特点的研究

木文以滇西北天然秃杉原始林为对象;通过对它的生长发育条件、分布、植物区系组成;群 落结构相个体发育过程进行了综合研究;证明目前滇西北的秃杉林多是树龄在300一500年的稀有珍贵群落。所谓千年老树仅为个别残存。     

Plant regeneration and protoplast culture of Browollia speciosa

Explants from hypcotyls and cotyledons of Browalia speciosa were shown to regenerate plantlets.Protoplasts were isolated from etiolated cotyledon material, and, although callus was readily obtained, plantlet regeneration was not observed using numerous hormone regimes.Abbreviations M Mannitol - 2,4-D Dichlorophenoxyacetic acid - NAA Naphthalene-acetic acid - BAP Benzylaminopurine - MS medium Murashige and Skoog (1962) medium - UM medium Uchimiya and Murashige (1974) medium - COT cotyledon - SH shoot - R root     

鸡胚部分去壳离体培养的效果与发育观察

将２５枚产后不到２４ｈ的种蛋,依次用碘酒和７０％酒精擦拭消毒处理,去掉其钝端外壳三分之一,然后用保鲜膜封口,并以２５枚正常种蛋作为对照,按常规方法进行孵化。重复实验３次。第１次孵化,实验组的孵化率显著低于对照组（Ｐ〈０．０５）,仅为４％;第２次孵化,两组的孵化率比较接近（分别为７２％和８０％）;第３次孵化,实验组的孵化率（７６％）稍低于对照组（８８％）。３次孵化的平均孵化率,实验组和对照组分别为６     

一种早期胚胎体外培养新方法的建立

建立一种不依赖于液体石蜡的早期胚胎体外培养方法——套皿法,并比较了4种不同处理方法体外培养胚胎的效果。结果显示,采用套皿法进行胚胎培养,盖液体石蜡和不盖液体石蜡皿中的胚胎在各阶段的发育率差异不显著。与套皿法相比,用单皿微滴覆盖法(Brinster法)培养的胚胎在各阶段的发育率显著降低。不覆盖液体石蜡单皿中的胚胎则阻断于二细胞阶段。实验设计的套皿法是一种有效的早期胚胎体外培养方法,为早期胚胎体外培养提供了一种新方法。     

Direct shoot regeneration from immature inflorescence cultures of <Emphasis Type="Italic">Chlorophytum arundinaceum</Emphasis> and <Emphasis Type="Italic">Chlorophytum borivilianum</Emphasis>

Direct shoot regeneration was achieved from immature inflorescence explants of Chlorophytum arundinaceum and C. borivilianum on half-strength Murashige & Skoog (MS) medium supplemented with 3.0 mg L⁻¹ BA, 150 mg L⁻¹ Ads, 0.1 mg L⁻¹ NAA and 3% (w/v) sucrose under a 16-h photoperiod. The shoot buds developed within 2–3 weeks of culture. High frequency of shoot bud regeneration was achieved when cultured on similar medium in subsequent subcultures. The apex portion (Type I) of the inflorescence produced more shoot buds as compared to the middle ones (type II). More than 75% of the terminal segment explants produced shoot buds within 4-week of culture. Response of basal portion (Type III) was negative for shoot bud initiation. Shoots rooted on half-strength basal MS medium supplemented with half-strength MS medium, 0.1 mg L⁻¹ IAA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house and successfully established in the soil where 90% of the plants survived. This protocol would be useful for commercial micropropagation and genetic improvement prograrmme.