山茱英的组织培养与植株再生

目的：建立山茱萸的组织培养及植株再生体系。方法：分别以山茱萸的叶片、花柄和花托为材料，进行山茱萸不同外植体的离体培养研究，筛选最佳培养基组成。结果：适宜山茱萸叶片愈伤组织诱导的培养基组合为1／2MS，附加BA2．0mg/L、IBA0，5—1．0mg／L；适宜山茱萸花柄、花托愈伤组织诱导的培养基组合为1／2MS，附加BA1．0mg／L、2，4-D0．5mg／L；在1／2MS附加BA2．0mg／L、IBA0．05mg／L的培养基上，可诱导不定芽的产生；1／2MS附加IBA2．0mg／L的培养基有利于山茱萸试管苗生根。讨论：山茱萸的花托是进行组织培养的最适外植体，白色或翠绿色、结构致密的愈伤组织较易分化产生不定芽。

Tissue culture and plant regeneration of Macrocarpium officinale

Objective:To establish a system d tissue culture and plant regeneration of Macrocarpium officinale.Methods:Different explants (leaf,floral shoot and floral receptacle)were cultured on different medium,and the best culture conditions were selected.Results:1/2 MS, supplemented BA 2.0mg/L and IBA 0.5-1.0mg/L,is the favorable medium for leaf callus induction.And 1/2MS,supplemented BA1.0mg/ L and 2,4-D 0.5mg/L is the favorable medium for induction of callus of floral shoot and floral receptacle.When the callus were cultured on 1/ 2MS medium,supplemented with BA 2.0mg/L and IBA0.05mg/L,the adventitious buds could formed.A lot of roots were obtained after the ad- ventitious buds were cultured on 1/2MS,supplemented with IBA 2.0mg/L.Conclusion:Floral receptacle is the best explants.Hard,white or dark-green callus can produce adventitious buds.