共查询到20条相似文献,搜索用时 250 毫秒
1.
Saccharomycescerevisiaeisanindustrialstrainwidelyusedintheproductionofethanol,breweryandsinglecellprotein(SCP).Butitisunabletofermentstarchduetothelackofamylolyticenzymes.Thestarchmustfirstbecooked,liquifiedandconvertedintoglucoseandthenutilizedincommer… 相似文献
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表达barstar基因及bar基因的转基因油菜的研究 总被引:30,自引:0,他引:30
从细菌Bacilusamyloliquefaciens染色体DNA中克隆了barnase抑制剂barstar的基因,构建了带有TA-29基因5′调控区(-1300-+3)与barstar基因编码区、CaMV35S启动子与除草剂抗性基因bar两个表达框架的植物表达质粒pBBS。以“双低”油菜“5-4”的子叶柄为受体,通过农杆菌介导的遗传转化,获得了在含有10mg/L卡那霉素和20mg/LPPT的筛选培养基上再生的转基因植株。PCR分析结果表明,barstar基因已整合到油菜染色体上;Northernblot检测表明,barstar基因及bar基因在转基因植物中得到了正确的调控与表达。以转基因油菜“5-4”为父本授粉给表达barnase基因的雄性不育植株,不育株能正常结实。 相似文献
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河南开封淀粉厂生态经济示范工程结构模型与投入产出模型的研究 总被引:1,自引:0,他引:1
河南开封淀粉厂生态经济示范工程结构模型与投入产出模型的研究刘玉萃,赵伟(河南农业大学,郑州450002)StructuralModelandInput-OutputModelofEcolomicalDemonstrationProjectinKaifengStarchFac-toryofHenanProvince¥.LiuYucui;ZhaoWei(HenanAgriculturalUniwrsity,Zhengzhou450002).Chi-neseJourualofEcology,1993,12(2):61-65.Basedontheintegrityofsystemandrelationshipbetweenvarioussubsystemsandemployingtransitionalstructuralmodel,themultilevelgradientstructuresofsystemarefoundfromtemporalandspatialdimen-sions.Accordingtothedissipateandreproductivefeaturesofecosystemandus 相似文献
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大鼠脑谷氨酸脱羧酶基因的cDNA克隆及序列分析 总被引:4,自引:0,他引:4
胰岛β-细胞自身抗原蛋白之一是脑中谷氨酸脱羧酶(Glutamicaciddecarboxylase,GAD,EC4.1.1.15)同源物,以双链cDNA为模权,用PCR方法快速克隆了Wistar大鼠脑GAD基因的cDNA将此包括编码593个氨基酸的全长DNA片段重组入pUC质粒并用双脱的氧末端终止法测定了全部序列,证明其全长为1779bp,经比较发现Wistar大鼠脑与Russell报导的大鼠脑G 相似文献
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大气苯酚对蚕桑急性伤害的初步研究潘如圭,管竟芳(江苏省植物研究所,南京210014)(江苏省浒关蚕种场,苏州215151)PreliminaryStudyontheAcuteInjuryofMulberrybyAtmosphericPhenol¥PanRugui(JiangsuInstituteofBotany,Nanjing210014).GuanJiangfang(HuguanSilkwormBreedingFornt,JiangsuProvince,Suzhun215151).ChineseJournalofEcology,12(5):49-51。Twoyearoldgraftedseedlingsofmulberryand4instarsilkwormaretestedunderfumigationwithdif-ferentconcentrationphenols。Theresultsshowthat,(1)afteracuteinjurybyphenol,leafproductionofmulberryixreducedby20%,leafqualityisdeterioratedwithade 相似文献
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Epilepsyisoneofthecommondiseasesduringchildhoodinclinicalpractice.Becauseofcomplexetiology,molecularmechanismsofepileptogenesisarenotfullyclear.Asweknowepilepsyiscausedbyimbalancebetweenexcitationandsuppressionofthebrain.Ithasbeenshowninhumanandanimalm… 相似文献
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1 厚叶瑞香 新种 图 1 :1~ 2DaphnepachyphyllaD .Fang,sp .nov.Fig .1 :1~ 2AD .xichouensiH .F .ZhouexC .Y .Changfoliorumlaminiscoriaceisvelcrassecoriaceissaepeinaequilaterissiccosuprarugosissubtussubbrunnescentibusjuventutemarginedenseciliatis,bracteismajoribus1 0~ 1 6mmlongisecil… 相似文献
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Insectsarethemostsuccessfulanimalgroupintermsofnumbersofspecies.Althoughmanyfactorsmustcontributetotheprofusionofinsectspecies,onekeyelementisprobablyadevelopmentalplanthatincorporatesmetamorphosisanddiapause.Diapauseisacomplexadaptativeresponsewhichi… 相似文献
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Xylanisamajorcomponentforminghemicelluloseandaccountsfor50%ofdryweightofherbaceousplants.Itssourcesareveryabundantintheworld.Xylanisfirstlyhydrolyzedintosmallmolecularxylose,andthenthexylosecanbeutilizedbymicrobesinmaterialcycle.Therearetwowaysforhydrol… 相似文献
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毛白杨COMT基因cDNA的克隆、序列与特异性表达分析 总被引:5,自引:0,他引:5
Ligninisanabundantnaturalproductiononlysecondtothecellulose ,asacellwallcomponentofallvascularplantsinbiosphere .Despiteitsimportantbiologicalsig nificanceinplants ,ligninisanundesirablecomponentinthechemicalprocessofpaperpulping ,andithasnegativeimpacto… 相似文献
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0 IntroductionGeneexpressioninorganismsisalteredduringdevel opmentandrespondtoenvironmentalchangesanddis ease .TheanalysisofspecificmRNAisofcentralimpor tanceinunderstandingfundamentalbiologicalprocesses .Northernblotanalysis ,insituhybridizationandre ver… 相似文献
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《人类学学报》2002,(Z1)
ActaAnthropologicaSinicaisajournaldealingwithanthropologicalandcloselyre latedsciences,mainlyinChinesewithshortEnglishabstracts.ThedetailedinformationprintedinitcannotbeeasilyusedbycolleagueswhoarenotabletoreadChinese .FortheconvenienceofthesecolleagueswemademoredetailedEnglishversionsforsomese lectedarticlespublishedinVolumes 1 9and 2 0ofourjournalandcollectedtheminthissupplementaryvolume .Readerscangetadditionalinformationforeachpaperbyconsult ingtheoriginalonepublishedinordinaryvolumesofourjournal.Weareg... 相似文献
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Itwasrecentlyevidencedthatmetabolicdisturbancesoffolicacidarecloselyrelatedtocardiovasculardiseasesandbirthdefects.5,10MTHFRisanimportantenzymeinthefolicacidmetabolicsystem.DecreaseinMTHFRactivitymayinducetheappearanceofhyperhomocysteinemia,whichmaycaus… 相似文献
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Hybridomaantibodiesareapowerfultoolforstudyingstructureandfunctionofproteinsowingtotheirabilitytorecognizeandbindtheircorrespondingantigensatspecialregions(i.e.epitope)withhighefficacyandspecificity.Monoclonalantibodies,especiallytheconformationdependen… 相似文献
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胰岛β-细胞自身抗原蛋白之一是脑中谷氨酸脱羧酶(Glutamicaciddecarboxylase,GAD,EC4.1.1.15)同源物。以双链cDNA为模板,用PCR方法快速克隆了Wistar大鼠脑GAD基因的cDNA,将此包括编码593个氨基酸的全长DNA片段重组入pUC质粒并用双脱氧末端终止法测定了全部序列,证明其全长为1779bp.经比较发现Wistar大鼠脑与Russell报导的大鼠脑GAD基因序列,有一处碱基的差别,但并不涉及氨基酸的改变。同时还对用PCR扩增长片段DNA进行了方法学上的探讨。 相似文献
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α1adrenergicreceptors(α1AR)belongtothesuperfamilyofGproteincoupledreceptors.Prolongedactivationofthesereceptorswouldleadtoadecreaseinagonistsensitivity.Theprocessmayberelatedtoreceptordownregulation,sequestrationandphospholation,etc.Desensitizationofα1BAR… 相似文献
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ProfessorWANGYing Lai,world renownedbiochemistandamemberoftheChineseAcademyofSciences ,passedawayat 19:10 ,May 5,2 0 0 1resultingfromanincurabledisease .Sincethen ,theChineseCommunistPartyhaslostanoutstandingmemberstrugglingallhislifeforthedevelopmentofscienceinC… 相似文献
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广西杜鹃花属新分类群 总被引:4,自引:0,他引:4
1 大橙杜鹃 新种 图 1 :1~ 7RhododendrondachengenseG .Z .Li ,sp .nov .(Subgen .Hymenanthes;Sect.Ponticum ;Subsect.Taliensia ;)SpeciesRh .haematodiFranch .affinis ,sedcorolliscampanulatis ,roseolisvelalbis ,5- 7-lobatis,staminibus 1 0 - 1 3;styloglabro ,calyxminoribusdensevelutinis ,lobis 5… 相似文献
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Pollengrainscausesomerespiratorysystemdiseasessuchasallergicasthmaandhayfever .Thesediseasesap pearespeciallyinfloweringperiodsof 相似文献