Cloning and expression of 5,10-Methylenetetrahydrofolate reductase (MTHFR) gene |
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| Authors: | CHEN GuanghuiZHANG ChenhuiZHANG XiaoyuZHU YanqingTANG JianMENG ZhaohengLI Zhu |
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| Institution: | (1) Institute of Cardiovascular Research, Beijing Medical University, 100083 Beijing, China;(2) Department of Health Care Epidemiology, Beijing Medical University, 100083 Beijing, China |
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| Abstract: | Hyperhomocysteinemia is an independent risk factor of cardiovascular diseases and birth defects. One of the important factors causing hyperhomocysteinemia is decrease of 5,10 Methylenetetrahydrofolate reductase. Human and rat MTHFR cDNAs with RT PCR were isolated, a prokaryodytic expression vector containing human MTHFR cDNA was constructed, and human MTHFR protein was expressed in \%E. coli.\% It was also found that the expression of rat MTHFR could be promoted by IL 1 and homocysteine. |
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| Keywords: | MTHFR cloning and expression |
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