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对蒺藜全草中-抗真菌甾体皂苷:替告皂苷元3-O-β-D.吡喃木糖(1→2)-[β-D-吡喃木糖(1→3)]-β-D-吡喃葡萄糖(1→4).[α-L-吡喃鼠李糖(1→2)]-β-D-吡喃半乳糖甙苷(TTS-12)进行提取分离工艺研究,采用70%乙醇提取后,沉淀部分经乙酸乙酯处理再进行硅胶柱层析,粗品重结晶后得到TTS-12纯品,HPLC-ELSD法测定TTS-12含量,TTS-12的收率为86.5%,产品纯度为97.1%。本工艺简便实用,收率稳定,产品纯度高,适合中试生产TTS-12。 相似文献
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肝素钠树脂精制工艺的研究 总被引:5,自引:0,他引:5
利用 D2 5 4 树脂可以将原始粗品肝素钠的效价提高到 179.8U SP U/m g,进一步纯化可得 2 0 1.5U SP U/m g的精品 ,其 H2 O2 用量 ( 1.5% )和氧化时间 ( 2 4 h)均少于传统工艺 ,产品光吸收和收率 ( 85.8% )最优 相似文献
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提高木薯循环培养的次生体胚再生植株频率研究 总被引:4,自引:1,他引:3
用木薯成熟体胚子叶作外植体诱导次生体胚,接种7d或15d后分别在诱导培养基或成熟培养基中加入AgNO3,可明显提高植株再生频率。前者可使体胚再生植株频率由对照的40.5%提高到58.1%;后者可使植株再生频率由对照的36.1%提高到61.3%。用ABA进行上述处理,效果更加显著。诱导期处理,可使植株再生频率由对照的40.5%提高到72.6%;成熟期处理,植株再生频率由对照的36.1%提高到81.3%。若诱导7d后,将2,4-D浓度由4.0mgL-1降至2.0mgL-1,并加AgNO3,继续诱导15d,转到附加0.25mgL-1ABA的成熟培养基中培养至30d,能极显著地促进体胚的发育和成熟,使植株再生频率达到一个相当高的水平,最高达95%,平均每个外植体产生的植株数达39.6个,分别相当于对照的2.38倍和2.46倍。 相似文献
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苦槛蓝提取物对小菜蛾的生物活性 总被引:4,自引:0,他引:4
以干扰作用控制指数(IIPC)为评价指标,小菜蛾为试虫,分别对苦槛蓝茎叶的不同溶剂提取物进行了生物活性测定,结果表明,石油醚和氯仿萃取部分具有较强的生物活性,而乙酸乙酯和乙醇萃取部分活性较低.在0.01gDW·ml^-1时,用石油醚和氯仿萃取物对小菜蛾处理1d后的产卵忌避率(ODR)分别为84.69%、79.90%,3d后为76.47%、45.70%,IIPC为0.1565和0.2055.在0.05gDW·ml^-1·L^-1时,1d后为88.52%和72.25%,3d后为87.33%和58.37%,IIPC为0.1125和0.2620.进而对氯仿部分进行了柱层析分离,并鉴定了3种黄酮类化合物,它们分别是5,7-二羟基黄烷酮(Ⅰ)、5,7-二羟基黄酮醇(Ⅱ)、3,4′-二甲氧基-5,7-二羟基黄酮醇(Ⅲ),其中Ⅱ在生测中表现出较好的活性。 相似文献
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本文以PEG磷酸酯/磷酸盐两水相系统,经两次萃取从重组大肠杆菌匀浆液中提取干扰素α1。最适萃取条件为22%PEG磷酸酯t 16%磷酸盐,3%NaCI,pH6.9。IFNαl的分配系数达到155,收率9g.6%,纯度提高25倍;对反萃取条件作了初步研究,较好的条件为20%PEG磷酸酯,10%磷酸盐,pH6.0,收率达到75.6%,但浓度有所稀释。与传统的提取方法相比,本法可省去高速离心去除细胞碎片的步骤,操作简单,能耗较低,而且收率较高,纯度也有所提高,最后IFNα1存在于磷酸盐相中,其中PEG含量仅为0.5%左右,这对后继纯化操作很有利。 相似文献
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低温条件下外源生理活性物质对棉铃发育的影响 总被引:7,自引:1,他引:6
和4月20日(正常播期)、6月15日(晚播)两个播期条件下.对棉花1~4果枝1、2果节棉铃及其对位果枝叶于花后15d和30d涂抹外源生理活性物质:6%蔗糖和0.6%符氨酰胺混合溶液(C+N)、2%蔗糖和0.2%谷氨酰胺混合溶液(1/3C+1/3N)及12%蔗精和1.2%谷氨酰胺混合溶液(2C+2N).各浓度处理的C/N比值相同。统计正常播期铃龄50d和纤维加厚发育期(铃龄25~50d)日均温分别为28.5℃、28.1℃.晚播铃龄50d和纤维加厚发育期日均温分刖为22.9℃、21.4℃(超过了相应的临界温度21℃和18℃)。试验结果表明.晚播条件下,花后15dC+N处理以及花后30d 2C+2N处理促进了氮和可溶性糖的运转.均使铃重增加最大,分别达0.40g和0.58g。3种浓度外源生理活性物质均增加了纤维素的累积量.且于花后30d促进了螺旋角(φ)和取向分布角(ψ)的优化.提高了纤维比强度.其中以2C+2N处理提高纤维比强度幅度最大.达1.45cN/tex。花后15d以1/3C+1/3N处理对提高纤维比强度最有利.达2.10cN/tex。 相似文献
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鱼腥藻SP.595液泡化原生质球诱导条件的研究 总被引:1,自引:0,他引:1
用浓度为0.15mol/L的KNO3、KCl、K2SO4、KH2PO4、K2HPO4、NaNO3、NaCl、Na2SO4、NaH2PO4、Na2HPO4、(NH4)2SO4、MgSO4、CaC12等单盐分别配合0.1%的溶菌酶处理鱼腥藻sp.595(Anabaenasp.595)。经4h,KH2PO4、K2SO4、Na2SO4、NaH2PO4、(NH4)2SO4、MgSO4、CaC12能诱导形成原生质球,但液泡化原生质球极少,而KNO3、NaNO3、NaC1诱导形成少量液泡化原生质球。用相近浓度的双盐,即KNO3和NaC1、KNO3和(NH4)2SO4、NaC1和(NH4)2SO4分别配合0.1%的溶菌酶处理,诱导效果亦不佳。用相近浓度的三盐NaC1、KNO3和(NH4)2SO4及五种盐NaC1、KNO3、(NH4)2SO4、Na2HPO4和KH2PO4分别配合0.1%的溶菌酶处理,诱导原生质球和液泡化原生质球的效果明显提高,液泡化原生质球比例达到66.90%—79.97%。 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
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Michael Hesse 《Plant Systematics and Evolution》1980,134(3-4):229-267
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout. 相似文献
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Surveillance of Class Ⅰ Newcastle Disease Virus at Live Bird Markets and Commercial Poultry Farms in Eastern China Reveals the Epidemic Characteristics 下载免费PDF全文
Xiaolong Lu Xiaoquan Wang Tiansong Zhan Yifan Sun Xin Wang Naiqing Xu Tianxing Liao Yu Chen Min Gu Shunlin Hu Xiaowen Liu Xiufan Liu 《中国病毒学》2021,36(4):818-822
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正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various 相似文献
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Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi 《中国病毒学》2019,34(1):102-105
<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV 相似文献