苦槛蓝提取物对小菜蛾的生物活性

以干扰作用控制指数(IIPC)为评价指标，小菜蛾为试虫，分别对苦槛蓝茎叶的不同溶剂提取物进行了生物活性测定，结果表明，石油醚和氯仿萃取部分具有较强的生物活性，而乙酸乙酯和乙醇萃取部分活性较低．在0．01gDW·ml^-1时，用石油醚和氯仿萃取物对小菜蛾处理1d后的产卵忌避率(ODR)分别为84．69％、79．90％，3d后为76．47％、45．70％，IIPC为0．1565和0．2055．在0．05gDW·ml^-1·L^-1时，1d后为88．52％和72．25％，3d后为87．33％和58．37％，IIPC为0．1125和0．2620．进而对氯仿部分进行了柱层析分离，并鉴定了3种黄酮类化合物，它们分别是5，7-二羟基黄烷酮(Ⅰ)、5，7-二羟基黄酮醇(Ⅱ)、3，4′-二甲氧基-5，7-二羟基黄酮醇(Ⅲ)，其中Ⅱ在生测中表现出较好的活性。

Bioactivity of Myoporum bontioides extracts to Plutella xylostella

The bioactivity of Myoporum bontioides extracts to Plutella xylostella was studied with IIPC as evaluated index. The results showed that petroleum ether and chloroform extracts had a higher activity than ethyl acetate and alcohol extracts. At 0.01 gDW x ml(-1), the ODR of petroleum ether and chloroform extracts was 84.69% and 79.90%, and 76.47% and 45.70% after treated 1d and 3d, while the IIPC was 0.1565 and 0.2055, respectively. Provided with a higher concentration of 0.05 gDW x ml(-1), the ODR was 88.52% and 72.25%, and 87.33%, 58.37%, while the IIPC was 0.1125 and 0.2620, respectively. From the chloroform extract of Myopdrum Bontioides, three flavonoids, 2, 3-dihydro-5, 7-dihydroxy-2-phenyl-4H-1-Benzopyran-4-one (I), 3, 5, 7-trihydroxy-2-phenyl-4H-1-Benzopyran-4-one (II) and 5, 7-dihydroxy-3-methoxy-2-(4-methoxyphenyl)-4H-1-Benzopyran-4-one (III), were isolated, and their structure were identified based on the analyses of physical and spectrum data. Among these compounds, (II) had a better bioactivity to Plutella xylostella.