灯盏花总黄酮对血小板聚集和血栓形成的影响

采用Ｂｏｍ氏比浊法测定灯盏花总黄酮（Ｅｒｉｇｅｒｏｎ ｂｒｅｖｉｓｃａｐｕｓ ｆｌａｖｏｎｅｓ,ＥＢＦ）在体内和体外对血小板活化聚集的影响。应用Ｋｏｈｌｅｒ等法观察ＥＢＦ对小鼠尾静脉注射花生四烯酸（ＡＡ）所致突然死亡的保护作用。结果表明,ＥＢＦ在体外和体内对ＡＤＰ、ＡＡ或血小板活化因子（ＰＡＦ）引起的血小板聚集均有明显抑制作用,体外实验,其效应呈浓度依赖关系,体内试验,于给药后１８０ｍｉｎ达最大抑     

EGFR反义脱氧寡核苷酸及其硫代修饰片段抑制人肝癌细胞系BEL…

本文旨在研究针对ＥＧＦＲ ｍＲＮＡ的反义寡核苷酸片段对ＢＥＬ－７４０４细胞ＥＧＦＲ基因表达及其对细胞生长的影响。合成了互补于ＥＧＦＲ基因５’起始编码区的２１聚脱氧寡核苷酸（ＯＤＮｓ）。实验结果显示,３．２ｕｍｏｌ／Ｌ ＯＤＮｓ明显抑制ＢＥＬ－７４０４细胞生长,「３Ｈ」Ｔｄｒ掺入抑制试验显示其对细胞ＤＮＡ合成的抑制作用表现剂量依赖性;密度扫描分析显示ＯＤＮｓ处理６、２４ｈ后,肝癌细胞ＥＧＦＲ ｍＲＮ     

体外培养条件下SCF、LIF与bFGF对昆明白小鼠精原干细胞增殖的影响

生长因子作为细胞体外培养和在体细胞生长及增殖必需的调节因子 ,一直被广泛的关注。业已证明干细胞因子(ＳｔｅｍＣｅｌｌＦａｃｔｏｒ,ＳＣＦ)、白血病抑制因子 (ｌｅｕｋａｅｍｉａｉｎｈｉｂｉｔｏｒｙｆａｃｔｏｒ,ＬＩＦ)和碱性成纤维细胞生长因子 (ＢｓａｉｃＦｉｂｒｏｂｌａｓｔＧｒｏｗｔｈＦａｃｔｏｒ,ｂＦＧＦ)具有刺激细胞增殖的作用[1～ 4] ,但大都是对单一因子进行研究。本实验探讨用这三种生长因子的不同组合观察对小鼠精原干细胞增殖的作用 ,以期定性和定量的探讨出体外培养初期三种因子对小鼠精原干细胞生长的影响 ,为小…     

rhGM—CSF对人骨髓粒单祖细胞集落形成及U937细胞分化的影响

本文应用造血祖细胞体外培养技术研究了重组人粒细胞－巨噬细胞集落刺激因子（ｒｈＧＭ—ＣＳＦ）对正常人骨髓粒单祖细胞集落（ＣＦＵ—ＧＭ）形成的影响,结果表明ｒｈＧＭ—ＣＳＦ在体外能促进细胞集落的形成,此种效应在一定范围内呈剂量依赖关系,与ＬＥＵＣＯＭＡＸ各剂量组相比无显著性差异。采用ＮＢＴ还原试验和ＡＰＡＡＰ法观察了ｒｈＧＭ—ＣＳＦ对Ｕ９３７细胞分化的影响,结果显示ｒｈＧＭ—ＣＳＦ能抑制Ｕ９３７细胞的增殖,促进其分化,部分细胞具有ＮＢＴ还原能力,ＣＤ１１６阳性细胞数增加。     

生长激素结合蛋白

动物的生长调控作用中垂体分泌的生长激素 (ＧＨ)起着重要的作用。ＧＨ的生理调控作用的发挥又与血液中的生长激素结合蛋白(ｇｒｏｗｔｈｈｏｒｍｏｎｅｂｉｎｄｉｎｇｐｒｏｔｅｉｎ ,ＧＨＢＰ)以及靶细胞膜上的生长激素受体 (ｇｒｏｗｔｈｈｏｒｍｏｎｅｒｅ ｃｅｐｔｏｒ,ＧＨＲ)的作用不可分割。这三者以及下丘脑的生长激素释放因子 (ＧＨｒｅｌｅａｓｅｆａｃ ｔｏｒ ,ＧＨＲＦ) ,还有垂体的生长抑素 (ｓｏｍａｔｏ ｓｔａｔｉｎ ,ＳＳ)等共同构成动物的内分泌生长轴(ｓｏｍａｔｏｔｒｏｐｉｃａｘｉｓ) ,对动物的生长发育起着决定性的…     

碱性成纤维细胞生长因子对胚胎神经细胞存活与生长的影响

本文利用大鼠胚胎中脑神经细胞作原代微团培养,培养物经不同浓度碱性成纤维细胞生长因子（ｂａｓｉｃ Ｆｉｂｒｏｂｌａｓｔ Ｇｒｏｗｔｈ Ｆａｃｔｏｒ,ｂＥＧＦ）,研究ｂＦＧＦ对细胞生长和分化的影响,并利用图象分析细胞形态的变化。结果表明ｂＥＧＦ可促进微团中的集落形成率明显增加,并显示量效应关系。图象显示ｂＦＧＦ可促进细胞神经突起增多,而且有丰富的神经纤维连结成网络状。认为ｂＥＧＦ能促进中脑细胞生长和分     

动脉平滑肌细胞sis／PDGF—B链的表达和调控

介绍平滑肌细胞ｓｉｓ／ＰＤＧＦ－Ｂ链表达和调控的进展。ｃ－ｓｉｓ原癌基因是ＰＤＧＦ－Ｂ的同源基因,将外源的ＰＤＧＦ基因导入哺乳类细胞是研究ＰＤＧＦ功能和调控的重要手段。内皮素ＩＬ、ＴＮＦ、血管紧张素Ⅱ和蛋白激酶Ｃ可调节ｓｉｓ基因的表达。ｓｕｒａｍｉｎ和新霉素的人工合成为拮抗ＰＤＧＦ效应提供广阔的前景。ｃ－ｓｉｓ可通过激活ｃ－ｍｙｃ,ｃ－ｆｏｓ等原癌基因而促进细胞增殖。     

腹腔注射乙酸钠对斑马鱼食欲调控基因及胰岛素分泌基因的影响

肠道微生物菌群发酵体内不易消化的碳水化合物可以产生乙酸。哺乳动物中,乙酸被报道可增加宿主食欲,改善葡萄糖平衡以及胰岛素敏感性。然而水产动物中乙酸盐相关作用及机理研究较少。以斑马鱼为研究对象,腹腔注射不同浓度乙酸钠发现,斑马鱼中促进摄食的基因表达量显著升高,而对抑制摄食基因的表达量没有显著影响,说明乙酸钠能够提高斑马鱼的食欲。另一方面,研究发现腹腔注射斑马鱼2 h、4 h、6 h后,肝脏及肠道促进胰岛素分泌基因表达水平也有显著性上升,说明乙酸钠对于胰岛素分泌具有促进作用。研究结果表明在水产动物中乙酸钠能够提高食欲并促进胰岛素的分泌,研究结果阐述了乙酸盐对水产动物生长的作用机理,以期为乙酸钠在生产中的应用提供依据。     

动脉平滑肌细胞sis／PDGF－B链的表达和调控

介绍平滑肌细胞ｓｉｓ／ＰＤＧＦ－Ｂ链表达和调控的进展。ｃ－ｓｉｓ原癌基因是ＰＤＧＦ－Ｂ的同源基因,将外源的ＰＤＧＦ基因导入哺乳类细胞是研究ＰＤＧＦ功能和调控的重要手段。内皮素、ＩＬ、ＴＮＦ、血管紧张素Ⅱ和蛋白激酶Ｃ可调节ｓｉｓ基因的表达。ｓｕｒａｍｉｎ和新霉素（ｎｅｏｍｙｃｉｎ）的人工合成为拮抗ＰＤＧＦ效应提供广阔的前景。ｃ－ｓｉｓ可通过激活ｃ－ｍｙｃ、ｃ－ｆｏｓ等原癌基因而促进细胞增殖。     

集落刺激因子与动脉粥样硬化

集落刺激因子（ＣＳＦｓ）可通过激活ＬＤＬ受体依赖性和非依赖性途径，使含ＡＰＯＢ－１００的脂蛋白水平降低。另一方面，动脉壁内ＣＳＦｓ则吸引循环单核细胞而聚集、衍化为巨噬细胞，并增强其摄取脂蛋白和酯化胆固醇的能力。提示ＣＳＦｓ对动脉粥样硬化可能具有抑制和促进双重作用。对ＷＨＨＬ兔的整体实验显示，Ｍ－ＣＳＦ能减缓其自发性动脉粥样硬化的进程。对ＣＳＦｓ的进一步研究可能有助于加深对动脉粥样硬化发病机理的认识，进而开辟防治新途径。     

壳聚糖酶的克隆表达与壳寡糖的制备分析

目的:克隆壳聚糖酶基因于大肠杆菌中实现高表达,制备壳寡糖。方法:以枯草芽孢杆菌总DNA为模板扩增壳聚糖酶基因(CSN),克隆至载体pET23a(+)上,转化菌株BL21(DE3)。重组子经0.5 mmol/L IPTG诱导后,SDS-PAGE和质谱检测与鉴定重组酶。酶纯化后水解壳聚糖,薄层色谱分析其水解产物。结果:质谱证明壳聚糖酶(31.5kDa)成功表达,表达量占菌体总蛋白的45%左右。纯化后重组酶浓度为900 mg/L,纯度95%、回收率85%,酶活力为10 000 U/mg。壳聚糖降解产物为壳二糖至壳四糖。结论:原核表达载体pET23a(+)-CSN构建正确,壳聚糖酶表达量与活性高,适用于水解壳聚糖制备壳寡糖。     

国内外蝗害治理技术现状与展望

本文首先概述了国内外蝗虫发生与为害的态势,总结了现阶段我国蝗虫发生与为害的主要特点:即农田飞蝗暴发频繁而且严重,草原土蝗的发生时常造成严重的经济损失,而且侵入城市干扰市民生活,我国与周边国家之间蝗虫过境迁移频繁,使用化学农药污染环境和农产品;分析了国内外蝗虫防治对策与技术的发展现状,重点介绍了应急防治和可持续治理对策、...     

Comparison of BMI and percentage of body fat of Indian and German children and adolescents

Today, serious health problems as overweight and obesity are not just constricted to the developed world, but also increase in the developing countries (Prentice 2006, Ramachandram et al. 2002). Focusing on this issue, BMI and percentage of body fat were compared in 2094 schoolchildren from two cross-sectional studies from India and Germany investigated in 2008 and 2009. The German children are in all age groups significantly taller, whereas the Indian children show higher values in BMI (e.g. 12 years: Indian: around 22 kg/m2; German: around 19 kg/m2) and in the percentage of body fat (e.g. 12 years: Indian: around 27%; German: around 18-20%) in most of the investigated age groups. The Indian children have significantly higher BMI between 10 and 13 (boys) respectively 14 years (girls). Indian children showed significant higher percentage of body fat between 10 and 15 years (boys) and between 8 and 16 years (girls). The difference in overweight between Indian and German children was strongest at 11 (boys) and 12 (girls) years: 70% of the Indian but 20% of the German children were classified as overweight. In countries such as India that undergo nutritional transition, a rapid increase in obesity and overweight is observed. In contrast to the industrialized countries, the risk of overweight in developing countries is associated with high socioeconomic status. Other reasons of the rapid increase of overweight in the developing countries caused by different environmental or genetic factors are discussed.     

Synthesis and turnover of cerebrosides and phosphatidylserine of myelin and microsomal fractions of adult and developing rat brain.

The synthesis and turnover of cerebrosides and phospholipids was followed in microsomal and myelin fractions of developing and adult rat brains after an intracerebral injection of [U-14C]serine. The kinetics of incorporation of radioactivity into microsomal and myelin cerebrosides indicate the possibility of a precursor-product relationship between cerebrosides of these membranes. The specific radioactivity of myelin cerebrosides was corrected for the deposition of newly formed cerebrosides in myelin. Multiphasic curves were obtained for the decline in specific radioactivity of myelin and microsomal cerebrosides, suggesting different cerebroside pools in these membranes. The half-life of the fast turning-over pool of cerebrosides of myelin was 7 and 22 days for the developing and adult rat brain respectively. The half-life of the slowly turning-over pool of myelin cerebrosides was about 145 days for both groups of animals. The half-life of the rapidly turning-over microsomal cerebrosides was calculated to be 20 and 40 h for the developing and adult animals respectively. The half-life of the intermediate and slowly turning-over microsomal cerebrosides was 11 and 60 days respectively, for both groups of animals. The amount of incorporation of radioactivity into microsomal cerebrosides from L-serine was greatly decreased in the adult animals, and greater amounts of the precursor were directed towards the synthesis of phosphatidylserine. In the developing animals, considerable amounts of cerebrosides were synthesized from L-serine, besides phosphatidylserine. The time-course of incorporation indicated that a precursor-product relationship exists between microsomal and myelin phosphatidylserine. The half-life of microsomal phosphatidylserine was calculated to be about 8 h for the fast turning-over pool in both groups of animals.     

Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats

Enterococcus faecalis was the most frequently isolated enterococcal species from anal swabs and tonsils of dogs and cats, although in the anal samples from dogs Ent. hirae was found almost as often as Ent. faecalis. Most Ent.faecium strains from dog tonsils differed from those associated with humans and other animals in that they fermented sorbitol. Typical Ent. avium as well as atypical Ent. avium -like strains were seen in dogs, while the related species Ent. raffinosus was associated with cat tonsils. Enterococcus cecorum also occurred mainly in cats. Certain atypical strains, presumptively identified as Ent. cecorum , shared characteristics with Ent. columbae.
The most frequent streptococcal species in tonsils of cats and dogs were Streptococcus suis and Strep. canis. Streptococcus canis and Strep. bovis predominated in anal swabs. The canine Strep. suis differed from the common porcine strains in fermenting mannitol.
Forty-seven of the 288 isolates examined could not be identified or related to known species. The characteristics of two groups of these bacteria, provisionally called 'Ton 31 group' and 'O7 group' are described.     

颧骨颧弓骨折的分类及诊治

颧骨颧弓骨折是最常见的面中部骨折之一,迄今为止,在其分类及诊治的选择上仍然存在一定争议,本文对目前颧骨颧弓骨折的分类及诊治的现状和进展作一综述,以期对临床工作提供参考。