| rhGM—CSF对人骨髓粒单祖细胞集落形成及U937细胞分化的影响 |
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| 引用本文： | 丁传林,侯云德.rhGM—CSF对人骨髓粒单祖细胞集落形成及U937细胞分化的影响[J].生物技术,1996,6(6):29-30,34. |
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| 作者姓名： | 丁传林 侯云德 |
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| 作者单位： | [1]南京医科大学微生物教研室 [2]中国预防医学科学院病毒研究所 |
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| 摘 要： | 本文应用造血祖细胞体外培养技术研究了重组人粒细胞－巨噬细胞集落刺激因子（ｒｈＧＭ—ＣＳＦ）对正常人骨髓粒单祖细胞集落（ＣＦＵ—ＧＭ）形成的影响，结果表明ｒｈＧＭ—ＣＳＦ在体外能促进细胞集落的形成，此种效应在一定范围内呈剂量依赖关系，与ＬＥＵＣＯＭＡＸ各剂量组相比无显著性差异。采用ＮＢＴ还原试验和ＡＰＡＡＰ法观察了ｒｈＧＭ—ＣＳＦ对Ｕ９３７细胞分化的影响，结果显示ｒｈＧＭ—ＣＳＦ能抑制Ｕ９３７细胞的增殖，促进其分化，部分细胞具有ＮＢＴ还原能力，ＣＤ１１６阳性细胞数增加。
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| 关 键 词： | rhGM－CSF 生物制品 CFU－GM 分化 研制 基因工程 |
Effects of rhGM-CSF on Granulocyte-macrophage Progenitor Cell Colony and U_(937) Cell Differentiation |
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| Ding Chuanlin, Yao Kun, Li Huandi et al.Effects of rhGM-CSF on Granulocyte-macrophage Progenitor Cell Colony and U_(937) Cell Differentiation[J].Biotechnology,1996,6(6):29-30,34. |
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| Authors: | Ding Chuanlin Yao Kun Li Huandi |
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| Abstract: | By using hematopoietic progenitor culture, effect of rhGM-CSF on bone marrow progenitor cell colony formation was assayed. The results showed that rhGM-CSF can inducenormal human bone marrow mononuclear cell to form colony of granulocyte and macrophage(CFU-GM) with concentration-dependent manner. This effect was nonsignificant different compared with LEUCOMAX (p>0. 05). With NBT reduction test and APAAP, rhGM-GSF was evaluated for its capacity to influence the differentiation of U937 cell. After 3 daysculture in the presence of rhGM-CSF, a small but significant proportion of U937 cell wereable to increased CD11b expression. |
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| Keywords: | rhGM-CSF Cell culture CFU-GM Differentiation |
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