HPLC法测定重组人干扰素α1b粉雾剂的含量

采用高效液相色谱法测定重组人干扰素α1b粉雾剂的含量及均匀度。结果表明溶液中重组人干扰素α1b浓度在92.50~462.50μg/ml时峰面积（Y）与相应浓度（X）呈线性关系,（r=0.999 9）。并且在成品的含量测定时,重组人干扰素α1b的色谱峰峰形良好,杂质与流动相无干扰。本方法精密度、准确度高,稳定性良好,能够准确、迅速的测定重组人干扰素α1b粉雾剂成品中的主药含量及均匀度。     

Membrane fusion: a new function of non steroidal anti-inflammatory drugs

Membrane fusion is an important event in many biological processes and is characterized by several intermediate steps of which content mixing between the two fusing vesicles signals the completion of the process. Fusion induced solely by small drug molecules is not a common event. Non Steroidal Anti-Inflammatory Drugs (NSAIDs), that control pain and inflammation, are also capable of exhibiting diverse functions. In this study we present a new function of NSAIDs belonging to the oxicam group, as membrane fusogenic agents. Small Unilamellar Vesicles (SUVs) formed by the phospholipid, dimyristoylphosphatidylcholine (DMPC), were used as model membranes. Fluorescence assays using terbium/dipicolinic acid (Tb/DPA) were used to monitor content mixing and corresponding leakage in presence of the drugs. Transmission Electron Microscope (TEM) was also used to image fusion bodies in drug treated vesicles as compared to the untreated ones. The results show that the three oxicam NSAIDs viz. Meloxicam, Piroxicam and Tenoxicam can induce fusion of DMPC vesicles and lead the fusion process to completion at a very low drug to lipid ratio (D/L) of 0.045. The oxicam drugs exhibit differential fusogenic behavior as reflected in the kinetics of content mixing and leakage, both of which can be described by a single exponential rate equation. Moreover, not all NSAIDs can induce membrane fusion. Indomethacin, an acetic acid group NSAID and ibuprofen, a propionic acid group NSAID, did not induce fusion of vesicles. This new property of NSAIDs has important applications in biochemical processes.     

Bringing scientists to the people--the Co-Extra website

Disseminating and facilitating access to science-based information is a necessity to enable the public to make informed decisions about appropriate uses of biotechnology products. It is also one of the major objectives of Co-Extra, a European-funded project addressing co-existence of genetically modified organisms and non-genetically modified organisms in Europe and their traceability. To this end, a dynamic and interactive website has been developed as the core element of the Co-Extra external communication strategy. This website has been designed to make it attractive and accessible to a large audience in a very simple and practical manner, building on practical experiences gained in the development of other websites related to biotechnology and genetically modified organisms. The website delivers popularized information for the general public as well as scientific data meant primarily for the more expert readers. It also provides for various permanent tools allowing multidirectional interaction with its visitors. Content is displayed using a web-based platform, based on a sophisticated Content Management System. First results indicate a high level of interest from the general public and from experts, showing that the content of this website can contribute by communicating science-based information to improve awareness and understanding of biotechnology.     

La^3＋对墨兰根状茎生长的调节作用

La~(3 )具有调节细胞膜透性,抑制糖和色素外渗、促进根对磷的吸收、促进根的发育等生理功能,但对其在植物生长和激素水平上调节作用的研究尚未见报道。本文以墨兰根状茎为材料,探讨了     

“秦油2号”油菜植株内含物与菌核病扩展的关系

通过对N、P、K不同水平的施肥条件下的“秦油2号”油菜植株内含物等与菌核病扩展间关系的研究,建立了27个数字模型,表明总氮、水溶性糖、含磷量、蛋氨酸、精氨酸、含水量与病斑扩展有密切关系,其次是甘氨酸、苏氨酸、谷氨酸、苹果酸和酰胺氮等．     

Influence of Moisture Content on Temperature Tolerance and Storage of Metarhizium flavoviride Conidia in an Oil Formulation

Investigations of the temperature tolerances of Metarhizium flavoviride conidia, of different moisture contents, in an oil formulation were carried out. The conidia were dried to 5, 9, 15 or 19% moisture contents and exposed to a range of temperatures, -15, 12, 25, 38, 48 and 55 C. Viability declined due to high temperatures and high moisture contents. After 4 months of storage, the conidia were at over 80% viability in all but the 19% moisture content group of the 25 C treatment and all the samples stored at lower temperatures. In a second experiment, conidia dried to 4-5% moisture content showed greater temperature tolerance than conidia with higher moisture contents. Short-term storage under adverse conditions could be feasible with conidia dried to a suitable degree.     

猕猴桃属植物果实营养成分的研究

本文报道猕猴桃属（Ａｃｔｉｎｉｄｉａ）３５个种类的果实的主要营养成分含量,猕猴桃果实维生素Ｃ的含量范围在１２．５４～１４０４．５２ｍｇ／１００ｇＦ．Ｗ,可溶性固形物含量范围在５．０％～１５．８％,总糖含量在０．９３％～９．０６％,总酸含量在０．２９％～２．５７％。果实最适宜的糖酸比为５～７,果实干物质１７种氨基酸含量范围在１．７９４％～９．０４％。文章还讨论了猕猴桃果实营养成分含量与开发利用价值的关系、果实的糖酸比与果实风味品质的关系、果肉汁液颜色与营养成分含量的关系等。     

几种不同进化程度动物细胞内质网超微结构的比较研究

应用稼射电镜技术,高锰酸钾固定扫描电镜技术及生化分离技术,比较研究了家兔、家鸽、蟾蜍,鲤鱼、脉红螺肝细胞和眼虫的内质网超微结构及含量。透射电镜观察结果显示：在高等哺乳动物肝细胞内质网丰富,以扁囊结构为主,在整个细胞质内均有分布,主要存在于核周围,并伴有伴随线粒体分布的特征;蟾蜍肝细胞内质网稀少,以长管状平行排列,分布在细胞质的局部,鲤鱼肝细胞内质网呈小泡状均匀分布在细胞质中,脉红螺肝细胞及眼虫细胞     

Novel bacterial lipoprotein structures conserved in low-GC content gram-positive bacteria are recognized by Toll-like receptor 2

Bacterial lipoproteins/lipopeptides inducing host innate immune responses are sensed by mammalian Toll-like receptor 2 (TLR2). These bacterial lipoproteins are structurally divided into two groups, diacylated or triacylated lipoproteins, by the absence or presence of an amide-linked fatty acid. The presence of diacylated lipoproteins has been predicted in low-GC content gram-positive bacteria and mycoplasmas based on the absence of one modification enzyme in their genomes; however, we recently determined triacylated structures in low-GC gram-positive Staphylococcus aureus, raising questions about the actual lipoprotein structure in other low-GC content gram-positive bacteria. Here, through intensive MS analyses, we identified a novel and unique bacterial lipoprotein structure containing an N-acyl-S-monoacyl-glyceryl-cysteine (named the lyso structure) from low-GC gram-positive Enterococcus faecalis, Bacillus cereus, Streptococcus sanguinis, and Lactobacillus bulgaricus. Two of the purified native lyso-form lipoproteins induced proinflammatory cytokine production from mice macrophages in a TLR2-dependent and TLR1-independent manner but with a different dependence on TLR6. Additionally, two other new lipoprotein structures were identified. One is the "N-acetyl" lipoprotein structure containing N-acetyl-S-diacyl-glyceryl-cysteine, which was found in five gram-positive bacteria, including Bacillus subtilis. The N-acetyl lipoproteins induced the proinflammatory cytokines through the TLR2/6 heterodimer. The other was identified in a mycoplasma strain and is an unusual diacyl lipoprotein structure containing two amino acids before the lipid-modified cysteine residue. Taken together, our results suggest the existence of novel TLR2-stimulating lyso and N-acetyl forms of lipoproteins that are conserved in low-GC content gram-positive bacteria and provide clear evidence for the presence of yet to be identified key enzymes involved in the bacterial lipoprotein biosynthesis.