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101.
Xinyue Ye Jian Wang Pengfei Xu Xiaoqian Yang Qixue Shi Genyan Liu Zhaoshi Bai Changlin Zhou Lingman Ma 《Microbial biotechnology》2023,16(9):1755-1773
Polymyxin resistance is conferred by MCR-1 (mobile colistin resistance 1)-induced lipopolysaccharide (LPS) modification of G− bacteria. However, the peptide MSI-1 exerts potent antimicrobial activity against mcr-1-carrying bacteria. To further investigate the potential role of MCR-1 in improving bacterial virulence and facilitating immune evasion, and the immunomodulatory effect of peptide MSI-1, we first explored outer membrane vesicle (OMV) alterations of mcr-1-carrying bacteria in the presence and absence of sub-MIC MSI-1, and host immune activation during bacterial infection and OMV stimulation. Our results demonstrated that LPS remodelling induced by MCR-1 negatively affected OMV formation and protein cargo by E. coli. In addition, MCR-1 diminished LPS-stimulated pyroptosis but facilitated mitochondrial dysfunction, further aggravating apoptosis in macrophages induced by OMVs of E. coli. Similarly, TLR4-mediated NF-κB activation was markedly alleviated once LPS was modified by MCR-1. However, peptide MSI-1 at the sub-MIC level inhibited the expression of MCR-1, further partly rescuing OMV alteration and attenuation of immune responses in the presence of MCR-1 during both infection and OMV stimulation, which can be exploited for anti-infective therapy. 相似文献
102.
Self incompatibility (SI) in Phalaris coerulescens is gametophytically determined by two unlinked multi allelic loci (S and Z). Neither the S nor Z genes have yet been cloned. As part of a map-based cloning strategy, high-resolution maps of the S and Z regions were generated from distorted segregating populations using RFLP probes from wheat, barley, oat, and Phalaris. The S locus was delimited to 0.26 cM with two boundary markers (Xwg811 and Xpsr168) and cosegregated with Xbm2 and Xbcd762. Xbcd266 was the closest marker linked to Z (0.9 cM). A high level of colinearity in the S and Z regions was found in both self-incompatible and -compatible species. The S locus was localized to the subcentromere region of chromosome 1 and the Z locus to the long arm end of chromosome 2. Several rice BAC clones orthologous to the S and Z locus regions were identified. This opens the possibility of using the rice genome sequence data to generate more closely linked markers and identify SI candidate genes. These results add further support to the conservation of gene order in the S and Z regions of the grass genomes. 相似文献
103.
专一识别脱落酸甲酯的单克隆抗体的制备与应用 总被引:2,自引:0,他引:2
专一识别2-顺(S)ABA甲酯的单克隆抗体来源于以ABA分子中的1-COOH为偶联位点合成的免疫原。它与游离态ABA和结合态ABA葡萄糖酯的交叉反应仅分别为1%与3.5%,而与ABA类似物,如2-顺-黄质醛、紫黄质以及ABA的2-反式异构体和(R)-对映体则无交叉反应。利用该抗体建立的高度灵敏和精确的ABAme酶联免疫测定法,其检测线性范围为0.048~1.52pmol。通过ABAmeELISA和GA1+3ELISA分析可知羊蹄叶片衰老与内源GA1+3/ABA比值的下降有关。 相似文献
104.
用基因定点突变法研究了白细胞介素-2(IL-2)中某些氨基酸对生物活性的影响。将IL-2中39Met和43Lys分别改为Pro,企图破坏此处α螺旋,突变体的CD图谱和生物活性均,不变,说明此处可能原来就不存在α螺旋.而将52Glu.53Leu,54Lys分别改为Pro后,CD谱发生了变化,生物活性也显著下降。表明这些氨基酸处在α螺旋中,将它们改为Pro后,影响了IL-2的结构,并导致活性下降 相似文献
105.
Repair activities of thymine radical anion by echinocoside, isolated from Pedicularis plicata. were studied using pulse radiolysis technique. The thymine radical anion was produced by the reaction of hydrated electron with thymine. Echinocoside. one of the polyphenols of phenylpropanoid glycoside, was added to the thymine aqueous solution saturated with N2. Kinetic analysis by transient absorption spectrum showed that thymine radical anion was formed at first, and then after several decades of microseconds of pulse radiolysis. the spectrum of thymine radical anion was changed to that of echinocoside radical anion. The evidence indicated that thymine radical anion was repaired through one-electron-transfer between the DNA base radical anion and echinocoside. The rate constant of electron transfer by echinocoside was 1.45× 109 dm3 · mol1 · s 1. 相似文献
106.
107.
108.
有机磷农药对土壤动物群落结构的影响研究 总被引:51,自引:3,他引:48
本文研究了有机磷农药废水灌溉对土壤动物群落结构的影响,并探讨了影响机理,结果表明,土壤动物种类和数量随着农药影响程度的增加而减少,在农药污染影响严重的1、2区,计有土壤动物21类和22类,动物平均密度为28571个/m^2和51269个/m^2,受中度和轻蔗污染影响的3、4区分别有35类和47类,平均密度为59285个/m^2,156587个/m^2。污染区土壤动物种类的减少主要由于常见类群和稀有 相似文献
109.
110.
中国异皮属线虫一新种(线虫纲:垫刃目:异皮科) 总被引:1,自引:0,他引:1
本文描述采自河北省滦县寄生于白茅Imperate cylindrica var.Major的异皮属线虫一新种,中华异皮线虫Heterodera sinensis sp.nov.;新种孢囊为柠檬形,阴门膜孔为两侧单膜孔型,下桥发达有泡状突;二龄幼虫口针基部球前部略凹陷,口孔侧唇外缘融合王头晕第1环纹内,即无明显侧唇,其体侧区有3条侧线与体环纹呈不完全网格状交叉;雄虫侧区有3条侧线与体环纹呈完全网格状 相似文献