Overproduction and purification of Escherichia colitRNA~(Leu)

ＡｓａｎａｄａｐｔｅｒｍｏｌｅｃｕｌｅｂｅｔｗｅｅｎｍＲＮＡａｎｄａｍｉｎｏａｃｉｄ,ｔＲＮＡｐｌａｙｓａｃｒｕｃｉａｌｉｎｔｅｒｍｅｄｉａｔｅｒｏｌｅｉｎｐｒｏｔｅｉｎｂｉｏｓｙｎｔｈｅｓｉｓ.ＴｈｅｉｎｔｅｒａｃｔｉｏｎｂｅｔｗｅｅｎｔＲＮＡａｎｄａｍｉｎｏａｃｙｌｔＲＮＡｓｙｎｔｈｅｔａｓｅ(ａａＲＳ)ｉｓｔｈｅｐｒｅｒｅｑｕｉｓｉｔｅｆｏｒａｃｃｕｒａｔｅｔｒａｎｓｌａｔｉｏｎ.Ｒｅｓｕｌｔｓｏｆｉｎｖｉｖｏａｎｄｉｎｖｉｔｒｏｅｘｐｅｒｉｍｅｎｔｓｉｎｄｉｃａｔｅｔｈａｔｔｈｅｓｐｅｃｉｆ…     

氨基酸集鱼剂与集鱼器

氨基酸集鱼剂与集鱼器范填基ＡｍｉｎｏＡｃｉｄＦｉｓｈＡｔｔｒａｃｔａｎｔｓａｎｄＡｔｔｒａｃｔｅｒ￥ＦａｎＺｈｅｎｊｉＡｂｓｔｒａｃｔｓ：ＴｈｉｓｐａｐｅｒｄｅｓｃｒｉｂｅｓｌｉｉｅｆｌｙｔｈｅｐｒｅｓｅｎｔｓｔａｔｕｓｏｆＡｍｉｎｏＡｃｉｄＦｉｓｈ...     

中国瘿螨亚科四新种记述（蜱螨亚纲：瘿螨总科）

本文记述中国瘿螨亚科４新种,它们是为害桃树的昌吉瘤瘿螨Ａｃｅｒｉａ ｃｈａｎｇｊｉｅｎｓｉｓ Ｋｕａｎｇ ｅｔ Ｐａｎｇ;为害垂柳的青海瘤瘿螨Ａｃｅｒｉａ ｑｉｎｇｈａｉｅｎｓｉｓ Ｋｕａｎｇ,垂柳刺子瘿螨Ａｃｕｌｏｄｅｓ ｓａｌｉｃｉｓＫｕａｎｇ和兰州狭点瘿螨Ｓｔｅｎａｃｉｓ ｌａｎｚｈｏｕｅｎｓｉｓ Ｋｕａｎｇ。模式标本保存在南京农业大学植物保护系。     

An ABA-binding protein with nucleic acid-binding property

ＴｈｅｐｌａｎｔｈｏｒｍｏｎｅＡＢＡｉｓｉｎｖｏｌｖｅｄｉｎｔｈｅｒｅｇｕｌａｔｉｏｎｏｆｐｌａｎｔｄｅｖｅｌｏｐｍｅｎｔａｎｄｐｈｙｓｉｏｌｏｇｉｃａｌｐｒｏｃｅｓｓｅｓ,ｓｕｃｈａｓｅｍｂｒｙｏｍａｔｕｒａｔｉｏｎａｎｄｔｈｅｒｅｓｐｏｎｓｅｏｆｖｅｇｅｔａｔｉｖｅｔｉｓｓｕｅｓｔｏｓｔｒｅｓｓｅｓ.Ｄｕｒｉｎｇｒｅｃｅｎｔｙｅａｒｓ,ｍｕｃｈｉｓｋｎｏｗｎａｂｏｕｔｔｈｅｍｏｌｅｃｕｌａｒｅｖｅｎｔｓｉｎＡＢＡｂｉｏｓｙｎｔｈｅｓｉｓａｎｄｔｈｅｒｅｇｕｌａｔｉｏｎｏｆｇｅｎｅｅｘｐｒｅｓｓ…     

Transient expression of hepatitis B virus surface antigen (HBsAg) and human erythropoietin (hEPO) genes in milk after direct introduction into ewe

Ｅｘｐｒｅｓｓｉｏｎｏｆｍｉｌｋｐｒｏｔｅｉｎｇｅｎｅｓｉｓｉｎｖｏｌｖｅｄｉｎａｈｕｇｅｎｅｔｗｏｒｋｏｆｒｅｇｕｌａｔｏｒｙｃｉｒｃｕｉｔｓｗｈｉｃｈａｒｅｌｉｎｋｅｄｔｏｔｈｅｉｎｔａｃｔｄｅｖｅｌｏｐｉｎｇｍａｍｍａｒｙｇｌａｎｄ,ａｎｄｈｏｍｅｏｓｔａｓｉｓｄｕｒｉｎｇｐｕｂｅｒｔｙ,ｐｒｅｇｎａｎｃｙ,ｌａｃｔａｔｉｏｎａｎｄｉｎｖｏｌｕｔｉｏｎ.Ａｎａｌｙｓｉｓｏｆｐｕｔａｔｉｖｅｒｅｇｕｌａｔｏｒｙｅｌｅｍｅｎｔｓａｎｄｈｙｂｒｉｄｇｅｎｅｉｎｔｉｓｓｕｅｃｕｌｔｕｒｅｓｙｓｔｅｍｓ…     

Epistasis plays an important role as genetic basis of heterosis in rice

Ｔｈｅｇｅｎｅｔｉｃｂａｓｉｓｏｆｈｅｔｅｒｏｓｉｓｉｓｓｔｉｌｌａｄｅｂａｔｉｎｇｉｓｓｕｅ.Ｔｗｏｈｙｐｏｔｈｅｓｅｓ,ｔｈｅｄｏｍｉｎａｎｃｅｈｙｐｏｔｈｅｓｉｓａｎｄｔｈｅｏｖｅｒｄｏｍｉｎａｎｃｅｈｙｐｏｔｈｅｓｉｓ,ｂｏｔｈｐｒｏｐｏｓｅｄｉｎ1908[1—3],ｈａｖｅｃｏｍｐｅｔｅｄｆｏｒｍｏｓｔｐａｒｔｏｆｔｈｉｓｃｅｎｔｕｒｙ.Ａｌｔｈｏｕｇｈｍａｎｙｒｅｓｅａｒｃｈｅｒｓｐｒｅｆｅｒｏｎｅｈｙｐｏｔｈｅｓｉｓｔｏｔｈｅｏｔｈｅｒ,ｅｘｐｅｒｉｍｅｎｔａｌｄａｔａａｌｌｏｗｉｎｇｆｏｒｃｒ…     

Cloning and high-level expression of plasminogen activator inhibitor-1 cDNA derived from human glomerular mesangial cells

Ｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒｉｎｈｉｂｉｔｏｒ1(ＰＡＩ1)ｉｓａｓｐｅｃｉｆｉｃｐｈｙｓｉｏｌｏｇｉｃａｌｉｎｈｉｂｉｔｏｒｏｆｕｒｏｋｉｎａｓｅｔｙｐｅｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒ(ｕＰＡ)ａｎｄｔｉｓｓｕｅｔｙｐｅｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒ(ｔＰＡ)[1].ＣｈａｎｇｅｓｏｆＰＡＩ1ｍａｙｉｎｄｕｃｅｉｍｂａｌａｎｃｅｂｅｔｗｅｅｎｇｌｏｍｅｒｕｌａｒｅｘｔｒａｃｅｌｌｕｌａｒｍａｔｒｉｘ(ＥＣＭ)ｓｙｎｔｈｅｓｉｓａｎｄｄｅｇｒａｄａｔｉｏｎ,ｔｈｕｓｌｅａｄｉｎｇ…     

天然药物信息(医学期刊扫描:文摘和评论)(英文)

Ｌ ＣａｒｎｉｔｉｎｅＩｍｐｒｏｖｅｓＥｘｅｒｃｉｓｅＴｏｌｅｒａｎｃｅＡｍｏｎｇＡｎｇｉｎａＰａｔｉｅｎｔｓｌｙｅｒＲＮ ,ＫｈａｎＡＡ ,ｅｔａｌ.Ｌ ｃａｒｎｉｔｉｎｅｍｏｄｅｒａｔｅｌｙｉｍｐｒｏｖｅｓｔｈｅｅｘｅｒｃｉｓｅｔｏｌｅｒａｎｃｅｉｎｃｈｒｏｎｉｃｓｔａｂｌｅａｎｇｉｎａ .ＪＡｓｓｏｃＰｈｙｓＩｎ ｄｉａ 48(11) :10 5 0～ 10 5 2 ,2 0 0 0 .Ｆｏｒｔｙ ｓｅｖｅｎｍｅｎａｎｄｗｏｍｅｎａｇｅｄ 48ｔｏ 6 4ｙｅａｒｓｗｈｏｈａｄｃｈｒｏｎｉｃｓｔａｂｌｅａｎｇｉｎａｔｏｏｋ 2ｇｏｆＬ ｃａｒ…     

天然药物信息(医学期刊扫描:文摘和评论)

Ｌ ＣａｒｎｉｔｉｎｅＩｍｐｒｏｖｅｓＥｘｅｒｃｉｓｅＴｏｌｅｒａｎｃｅＡｍｏｎｇＡｎｇｉｎａＰａｔｉｅｎｔｓｌｙｅｒＲＮ ,ＫｈａｎＡＡ ,ｅｔａｌ.Ｌ ｃａｒｎｉｔｉｎｅｍｏｄｅｒａｔｅｌｙｉｍｐｒｏｖｅｓｔｈｅｅｘｅｒｃｉｓｅｔｏｌｅｒａｎｃｅｉｎｃｈｒｏｎｉｃｓｔａｂｌｅａｎｇｉｎａ .ＪＡｓｓｏｃＰｈｙｓＩｎ ｄｉａ 4 8(11) :10 5 0～ 10 5 2 ,2 0 0 0 .Ｆｏｒｔｙ ｓｅｖｅｎｍｅｎａｎｄｗｏｍｅｎａｇｅｄ 4 8ｔｏ 6 4 ｙｅａｒｓｗｈｏｈａｄｃｈｒｏｎｉｃｓｔａｂｌｅａｎｇｉｎａｔｏｏｋ 2 ｇｏｆＬ ｃ…     

天然药物信息(医学期刊扫描:文摘和评论)(英文)

ＮａｔｕｒａｌＭｅｄｉｃｉｎｅＩｎｆｏｒｍａｔｉｏｎ(ＭｅｄｉｃａｌＪｏｕｒｎａｌＷａｔｃｈ :ＡｂｓｔｒａｃｔａｎｄＣｏｍｍｅｎｔｓ)Ｌ ＣａｒｎｉｔｉｎｅＩｍｐｒｏｖｅｓＥｘｅｒｃｉｓｅＴｏｌｅｒａｎｃｅＡｍｏｎｇＡｎｇｉｎａＰａｔｉｅｎｔｓｌｙｅｒＲＮ ,ＫｈａｎＡＡ ,ｅｔａｌ.Ｌ ｃａｒｎｉｔｉｎｅｍｏｄｅｒａｔｅｌｙｉｍｐｒｏｖｅｓｔｈｅｅｘｅｒｃｉｓｅｔｏｌｅｒａｎｃｅｉｎｃｈｒｏｎｉｃｓｔａｂｌｅａｎｇｉｎａ .ＪＡｓｓｏｃＰｈｙｓＩｎ ｄｉａ 4 8(11) :10 5 0～ 10 5 2 ,2 0 0 0 .Ｆｏｒｔｙ ｓ…     

Integration of the study of natural and anthropogenic disturbances using severity gradients

Disturbance is an integral part of every ecosystem, but humans are altering disturbance regimes in fundamental ways that can alter outcomes for ecosystem structure and function. Fortunately, advances in understanding ecosystem responses to natural disturbances can address the ecological consequences of the novel suite of disturbances now created by humans. Complex interactions among both natural and anthropogenic disturbances at many overlapping spatial and temporal scales can be examined across severity gradients. The gradient approach applies ecological tools to differential conditions of stability and fertility, degrees of biological legacy and rates of successional recovery and can help address modern concerns about socio‐economic consequences of disturbance and the sustainability of ecosystem services.     

A Non-native Perennial Invades a Native Forest

Disturbance is frequently a requirement for non-indigenous plant invasions, but plants that invade in the absence of significant disturbance pose special problems for conservationists and land stewards, as the invasion rates and effects are difficult to predict. Knowledge of the invader's population ecology is essential for the preservation of native plant communities. A sub-shrub native to South America, Tibouchina herbacea (Melastomataceae) is invading intact, wet forests and open, disturbed sites on the islands of Hawaii and Maui. Experimental tests of the importance of disturbance for reproductive rates showed that absence of canopy cover produced the highest rates of germination, survival and seed dispersal distance. Ground cover was less important, but had a positive effect on germination and a negative effect on survival. Results of these experiments suggest that T. herbacea will probably spread more quickly in open, disturbed areas, but is very capable of establishment in natural forests. If spread is arrested in the early stages, manual control may be effective, otherwise landscape-level control efforts, such as biological control, will be needed. Prevention of dispersal and control of T. herbacea in forested areas should be a priority for land managers in Hawaii.     

Beyond the patch: Disturbance affects species abundances in the surrounding community

The role of disturbance in community ecology has been studied extensively and is thought to free resources and reset successional sequences at the local scale and create heterogeneity at the regional scale. Most studies have investigated effects on either the disturbed patch or on the entire community, but have generally ignored any effect of or on the community surrounding disturbed patches. We used marine fouling communities to examine the effect of a surrounding community on species abundance within a disturbed patch and the effect of a disturbance on species abundance in the surrounding community. We varied both the magnitude and pattern of disturbance on experimental settlement plates. Settlement plates were dominated by a non-native bryozoan, which may have established because of the large amount of initial space available on plates. Percent covers of species within the patch were affected by the surrounding community, confirming previous studies' predictions about edge effects from the surrounding community on dynamics within a patch. Disturbance resulted in lower percent cover in the surrounding community, but there were no differences between magnitudes or spatial patterns of disturbance. Disturbance lowered population growth rates in the surrounding community, possibly by altering the abiotic environment or species interactions. Following disturbance, the recovery of species within a patch may be affected by species in the surrounding community, but the effects of a disturbance can extend beyond the patch and alter abundances in the surrounding community. The dependence of patch dynamics on the surrounding community and the extended effects of disturbance on the surrounding community, suggest an important feedback of disturbance on patch dynamics indirectly via the surrounding community.     

FITNESS TRADE-OFFS MODIFY COMMUNITY COMPOSITION UNDER CONTRASTING DISTURBANCE REGIMES IN PSEUDOMONAS FLUORESCENS MICROCOSMS

Disturbance is thought to be a major factor influencing patterns of biodiversity. In addition, disturbance can modify community composition if there are species specific trade-offs between fitness and disturbance tolerance. Here, we examine the role of disturbance on the evolution of coexisting biofilm-forming morphotypes of Pseudomonas fluorescens maintained in spatially structured laboratory microcosms. We identified four heritably stable ecotypes that varied significantly in their competitiveness under different disturbance treatments. Furthermore, we identified significant trade-offs in competitiveness across disturbance treatments for three of four of these ecotypes. These trade-offs modified dominance relationships between strains and thus altered community composition, with a peak of ecotype diversity occurring at intermediate disturbance frequencies.     

Migratory Disturbance Thresholds with Mule Deer and Energy Development

Fine-scale movement data has transformed our knowledge of ungulate migration ecology and now provides accurate, spatially explicit maps of migratory routes that can inform planning and management at local, state, and federal levels. Among the most challenging land use planning issues has been developing energy resources on public lands that overlap with important ungulate habitat, including the migratory routes of mule deer (Odocoileus hemionus). We generally know that less development is better for minimizing negative effects and maintaining habitat function, but we lack information on the amount of disturbance that animals can tolerate before reducing use of or abandoning migratory habitat. We used global positioning system data from 56 deer across 15 years to evaluate how surface disturbance from natural gas well pads and access roads in western Wyoming, USA, affected habitat selection of mule deer during migration and whether any disturbance threshold(s) existed beyond which use of migratory habitat declined. We used resource and step selection functions to examine disturbance thresholds at 3 different spatial scales. Overall, migratory use by mule deer declined as surface disturbance increased. Based on the weight of evidence from our 3 independent but complementary metrics, declines in migratory use related to surface disturbance were non-linear, where migratory use sharply declined when surface disturbance from energy development exceeded 3%. Disturbance thresholds may vary across regions, species, or migratory habitats (e.g., stopover sites). Such information can help with management and land use decisions related to mineral leasing and energy development that overlap with the migratory routes of ungulates. © 2020 The Wildlife Society.     

An ecological perspective in aquatic ecotoxicology: Approaches and challenges

This paper aims to highlight the considerable potential of a better integration of ecological theory in aquatic ecotoxicology. It outlines how community ecology, studies on trophic interaction and disturbance ecology could provide an enhanced theoretical basis for aquatic ecotoxicology and increase ecological relevance in environmental risk assessment of chemicals. Based on the literature and own research, approaches from aquatic ecotoxicology are presented, which are based on ecological considerations and address a higher level of biological complexity for risk assessment strategies of chemicals. The concepts of species-sensitivity distribution (SSD), pollution-induced community tolerance (PICT), the use of model ecosystems and the sediment quality triad (SQT) in ecological risk assesment as well as inputs from ecotoxicology into landscape ecology are illustrated. These examples aim to evidence aquatic ecotoxicology as a rewarding field of ecological research.     

基于景感生态学理论的湿地公园规划设计实践——以长垣市王家潭湿地公园为例

结合生态学基本原理，综合分析各类要素与感知，并以可持续发展为目标研究土地利用规划、建设与管理的"景感生态学"是湿地公园规划设计的重要指导理论，综合景感生态学与湿地规划设计两方面的研究构建了基于景感生态学理论的湿地公园规划设计路径框架。以河南省长垣市王家潭湿地公园为例，探索了基于景感生态学理论开展湿地公园规划设计的过程中，结合生态学原理与人类生理感知、心理感受进行协调环境、社会、经济三大效益的规划设计。以"源-汇"理论划定生态功能主分区，采用集聚间有离析模式结合现状设施、景观效果、地脉文脉传承进行空间边界调整，形成整体空间格局；以生态岸线发育系数、地形旷奥度结合观赏体验、空间感知创造动植物优良生境，构建湿地公园生境基底；以生态位宽度为重要指标并结合民风民俗、季相策略配置湿地公园中的优势种、伴生种采用近自然造林方式完成植物规划设计；以适度干扰原理，采用轻干扰和产景融合策略规划人类社会、经济活动的介入；同时以"智慧公园"体系初步构建物联网系统采集各类信息数据，构成信息循环反馈、场地时序更新的渐善式模型，推动场地的持续完善。从理论路径的构建、空间格局的形成、生境基底的打造、植物种群的规划、人类活动的介入、"智慧公园"的构建等角度为景感生态学理论在湿地公园中的运用提供指引。     

高速发展的景观生态学

景观生态学是生态学中一门年轻的分支学科,它的理论与方法和传统生态学有着本质的区别,它注重人类活动对景观格局与过程的影响.景观生态学为综合解决资源与环境问题提供了新的理论和方法,因而近年来受到高度重视.从景观生态学的理论框架、一般原理、研究方法和实际应用四个方面进行论述.     

转型期城市生态学前沿研究进展

城市是一类以人类活动为中心听社会－经济－自然复合生态系统。城市人类活动对局地、区域和全球环境的胁迫效应,自然生态系统的响应机制,城市时、空、量、构、序的耦合规律、动力学机制和控制论方法是当前国际社会和学术界关注的热点。介绍了转型期城市人类生态影响研究的一些主要国际科学计划,如ＳＣＯＰＥ及ＩＨＤＰ等,综述了城市生态学研究三大前沿领域的国际研究动向和案例,即人居生态学、产业生态学和城镇生命支持系统生态     

MINI-REVIEW Assessment of Body Image Disturbance in Obesity

Disturbance in body image has long been noted as one of the most distressing psychological factors for obese individuals. Yet, only in recent years have researchers and clinicians begun to appreciate the need to integrate systematic evaluation of this component into intervention programs. Accordingly, this mini-review offers an overview of selected strategies for the assessment of body image disturbance in obesity.