蝇蛆养殖

随着我国养殖业的迅速发展 ,对高蛋白饲料需求量越来越大。传统的高蛋白饲料多以鱼粉为主 ,最近几年世界鱼粉市场由于产量减少而消费量增加 ,价格不断上涨。现在人们把饲养昆虫作为解决蛋白饲料来源的主攻方向 ,其中蝇蛆资源的开发利用最为引人关注。家蝇 ( Musca domestica)属昆虫纲、双翅目、环裂亚目、家蝇科 ,是世界性分布的蝇种 ,繁殖力高 ,适应性强 ,常用作实验性蝇种和开发利用对象。中科院昆明动物研究所对家蝇幼虫营养成分的分析结果表明 :新鲜幼虫和幼虫干粉的粗蛋白略高于鲜鱼、鱼粉及肉骨粉 ,并含有多种微量元素 ,氨基酸含量也…     

食物中多不饱和脂肪酸在家蝇幼虫体内的富集与代谢及对其生长的影响

【目的】阐明家蝇 Musca domestica 幼虫对食物中各种多不饱和脂肪酸的富集能力以及代谢转化情况,并探究各种多不饱和脂肪酸对家蝇幼虫生长的影响。【方法】在基础饲料中添加不同浓度(3%, 6%和12%)的多不饱和脂肪酸(亚油酸、α-亚麻酸、花生四烯酸和二十二碳六烯酸)饲养经过脱脂传代培养的家蝇幼虫;提取家蝇幼虫的总脂肪酸,利用气相色谱仪进行检测和分析;测定统计幼虫体重,以分析多不饱和脂肪酸对家蝇幼虫生长的影响。【结果】亚油酸、α-亚麻酸和花生四烯酸在家蝇幼虫体内均能被富集,且它们的富集程度随着食物中多不饱和脂肪酸的添加浓度的升高而增加,其中亚油酸、α-亚麻酸和花生四烯酸在幼虫体内富集的最高含量(占体内总脂肪酸的比例)分别为21.93%, 16.13%和9.68%,而二十二碳六烯酸不能在家蝇幼虫体内富集,提示家蝇幼虫食物中添加的各种多不饱和脂肪酸经过代谢后并没有在其体内产生新的脂肪酸,而食物中添加的二十二碳六烯酸在家蝇幼虫体内被分解代谢后消除。饲喂α-亚麻酸及花生四烯酸后家蝇幼虫体重增长较为明显,其中6%α-亚麻酸添加组的幼虫体重显著高于对照组(取食脱脂饲料)和3%和12%α-亚麻酸添加组,3%和6%花生四烯酸添加组的幼虫体重显著高于对照组和12%花生四烯酸添加组。【结论】家蝇幼虫体内能够从食物中富集部分多不饱和脂肪酸,多不饱和脂肪酸碳链越长其富集程度越低直至不能富集,富集的多不饱和脂肪酸对家蝇幼虫生长有不同程度的影响。     

蝇蛆蛋白粉对凡纳滨对虾生长的影响

设计了4种实验饲料(ABCD)并进行了8周饲养试验来评定添加蝇蛆粉对凡纳滨对虾生长性能的影响.以实用饲料D作为对照组,ABC是在D的基础上,分别以5%,10%和15%的蝇蛆粉替代鱼粉.养殖试验结束后,分别计算体长、全长和背甲宽等生长性能.实验结果显示,AB组凡纳滨对虾的生长性能与对照组差异不显著(P>0.05).C组与对照组差异极显著(P<0.01).结果表明,饲料中添加适量的蝇蛆蛋白粉对凡纳滨对虾有一定的促生长作用,可用适量的蝇蛆蛋白粉替代对虾配合饲料中的部分鱼粉.     

鸡肉粉完全替代鱼粉饲料中补充包被氨基酸对凡纳滨对虾生长、体成分及组织氨基酸含量的影响

为了探讨鸡肉粉完全替代鱼粉时饲料氨基酸的平衡性以及外源氨基酸的添加方式与凡纳滨对虾生长、体成分、血浆游离氨基酸及肌肉氨基酸含量的关系, 本试验采用26因子试验设计进行了为期56d的饲养试验。2个饲料蛋白质水平分别为40%和32%, 6个饲料处理分别为鱼粉组(对照组)、鸡肉粉组、鸡肉粉+晶体EAA组、鸡肉粉+晶体EAA+晶体NEAA组、鸡肉粉+包被EAA组、鸡肉粉+包被EAA+包被NEAA组, 配制12组饲料。将凡纳滨对虾(0.300.01) g随机分配到36个圆桶(150 L)中, 每桶30尾, 每3个桶为一个处理组, 饲喂一种饲料, 每天饱食投喂三次。在每一饲料蛋白质水平下, 无论是补充晶体氨基酸(CAA)组还是包被氨基酸组对虾的增重率均显著高于鸡肉粉组(P0.05), 且在32%蛋白质水平下, 包被EAA组对虾增重率达到了鱼粉组水平(P0.05); 补充晶体EAA+NEAA组对虾增重率与补充晶体EAA组无差异(P0.05), 但均显著低于补充包被氨基酸组(P0.05); 补充包被EAA组对虾增重率显著高于补充包被EAA+NEAA组(P0.05)。饲料系数的变化正好与增重率变化相反(P0.05)。饲喂高蛋白质水平饲料较之饲喂低蛋白质饲料明显提高对虾增重率、虾体蛋白含量(P0.05), 但降低虾体脂肪含量(P0.05)。包被氨基酸组凡纳滨对虾血浆游离氨基酸含量总体显著低于CAA组(P0.05)。除谷氨酸、甘氨酸以及脯氨酸外, 各组对虾肌肉氨基酸含量无显著差异(P0.05)。结果表明, 在32%饲料蛋白质水平下, 用鸡肉粉完全替代鱼粉时, 饲料中补充包被EAA可明显促进凡纳滨对虾的生长, 且达到了鱼粉组的饲喂效果。     

家蝇幼虫对两种秸秆的消化及利用研究

本文旨在探明家蝇Musca domestic幼虫对高粱秸秆和小麦秸秆营养成分利用和木质纤维素的降解情况,以及不同配比秸秆饲料对家蝇生物学指标的影响,为今后产业化利用家蝇来生物降解秸秆类有机废弃物奠定理论基础.本研究采用70％高粱秸秆粉、70％麦秸秆粉和纯麦麸作为饲料,每种饲料各设置对照组、发酵组和家蝇幼虫取食组3种处理,检测并分析3种饲料各处理组中一般营养成分和木质纤维素的含量及变化;同时采用7种配比饲料喂养家蝇,观察各组家蝇不同发育阶段的体长、体重、存活率和发育历期等.结果表明:(1)3种饲料的各处理组中,一般营养成分和木质纤维素的含量均呈现:对照组>发酵组>家蝇取食组的趋势,此外除高粱秸秆组粗蛋白外,其余各组成份均差异显著(P<0.05);(2)小麦秸秆的木质纤维素含量>高粱秸秆>麦麸,且各组间均差异显著(P<0.05);(3)在相同秸秆配比条件下,高粱秸秆饲喂的家蝇其各虫态体长、体重、总存活率和发育历期均要优于麦秸秆喂养,且总存活率和发育历期与麦麸喂养相比差异不显著(P>0.05);(4)纯高粱秸秆喂养下家蝇仍能保持正常的生长发育,而在90％小麦秸秆喂养下,家蝇则无法存活.综上所述,家蝇适合作为生物转化器用于秸秆、尤其是高粱秸秆的降解,且降解效果优于单纯的微生物发酵降解,研究结果为今后规模化利用家蝇降解秸秆类有机废弃物提供了理论依据和科学基础.     

家蝇幼虫组织匀浆液的抗病毒活性

以家蝇Musca domesticaL.幼虫为材料,研究了蝇蛆组织匀浆液的抗病毒活性。用鸡胚培养检测了其抗流感病毒的活性,乙型肝炎病毒表面抗原诊断试剂盒检测了其对乙型肝炎病毒表面抗原的破坏作用。结果表明:15μL的家蝇幼虫组织匀浆液对流感病毒的抑制率为72.41%,而30,50和80μL剂量组对病毒的杀灭率都达到80%以上;蝇蛆组织匀浆液对乙型肝炎病毒表面抗原的破坏率可达到87.5%。说明家蝇幼虫体内存在着抗病毒活性物质。这为开发天然的抗病毒药物提供了新的途径。     

烟青虫人工饲料的改进研究

在原有饲料配方的基础上,通过添加适宜浓度的辣椒粉作为取食刺激剂及去除原饲料配方中的番茄酱组分,作者对烟青虫Helicoverpa assulta(Guenée)幼虫的人工饲料进行了改进研究。测定了6种人工饲料对烟青虫生物学参数、营养指标和生命表参数的影响。结果表明,与取食对照饲料相比,取食添加3%番茄酱的饲料后,烟青虫的幼虫历期、蛹重和生殖力无显著差异。在试验浓度内,取食添加辣椒粉的饲料后烟青虫幼虫存活率、幼虫相对取食量和蛹重均显著高于取食对照饲料的相应指标。但饲料中辣椒粉浓度达到或超过2%时烟青虫幼虫的消化能力降低,达到4%时成虫交配力显著降低。在6种供试饲料中,配方Ⅱ即添加1%辣椒粉的饲料为最佳配方饲料,与取食其他饲料相比,取食配方Ⅱ饲料后烟青虫生殖力和内禀增长率均显著提高。改进的人工饲料可大量饲养烟青虫以满足室内试验及工厂化生产核多角体病毒的需要。     

家蝇幼虫营养成分的分析及利用

本文以多种分析手段,对家蝇幼虫与其他几种饲料的营养成分做了对比分析。新鲜幼虫和幼虫干粉的粗蛋白分别为15.62％和60.88％,略高于鲜鱼、鱼粉及肉骨粉,并含有多种微量元素。对氨基酸组成进行了比值计算:E％=44.09％、E/N=0.79、E/T=2.94。其中必需氨基酸总量是鱼粉的2.3倍,MET(蛋氨酸)是2.7倍,LYS(赖氨酸)是2.6倍,均明显高于鱼粉。     

四种昆虫饵料对穴蚁蛉幼虫生长发育及消化利用的影响

选择4种可规模化饲养的昆虫——米蛾Corcyra cephalonica、家蝇Musca domestica、斜纹夜蛾Spodoptera litura、甜菜夜蛾Spodoptera exigu幼虫来饲养穴蚁蛉Myrmeleon sagax(Walker)幼虫(俗称蚁狮),研究这4种饵料对蚁狮生长发育及消化利用的影响。结果显示,用家蝇幼虫饲养的蚁狮,其体重增长、相对生长率、化蛹率、蛹重均显著高于用斜纹夜蛾和甜菜夜蛾幼虫饲养的蚁狮,而幼虫历期则比斜纹夜蛾和甜菜夜蛾幼虫饲养的短;用米蛾幼虫饲养,虽然蚁狮体重增长、化蛹率和蛹重与用家蝇幼虫饲养的差异不显著,但其相对生长率却显著低于用家蝇幼虫饲养的蚁狮,幼虫历期也比用家蝇幼虫饲养的明显延长。同时食物消化利用的结果显示,用4种饵料饲养蚁狮,它们的近似消化率差异不显著,但食物利用率和食物转化率均以家蝇幼虫饲养的蚁狮最高。经分析比较,在4种饵料昆虫中,以用家蝇幼虫每4d喂蚁狮1次的饲养效果最佳。     

染料标记对斜纹夜蛾生长发育及成虫飞行能力的影响

以斜纹夜蛾Spodoptera litura为研究对象,将染料添加到半人工饲料中饲喂幼虫,评价其对该虫生长发育和成虫飞行能力的影响,以期为开展该虫的田间诱集和扩散行为研究提供标记方法。结果表明,添加不同浓度苏丹红7B、苏丹蓝Ⅱ和龙胆紫的半人工饲料饲养斜纹夜蛾幼虫,含0.006%苏丹红7B的半人工饲料幼虫存活率高,成虫标记效果好。进一步研究显示,取食含0.006%苏丹红7B半人工饲料的斜纹夜蛾幼虫发育历期、蛹重以及成虫寿命、体长、翅展及飞行能力等与对照相比均无显著差异。含0.006%苏丹红7B半人工饲料饲料对斜纹夜蛾的生物学无显著影响,标记效果显著,操作简单,适合于大规模标记斜纹夜蛾成虫。     

Fly pupae as attractant carriers for toxic baits for red imported fire ants (Hymenoptera: Formicidae)

Eight laboratory-reared ant species were fed baits of house fly, Musca domestica L., pupae treated with hydramethylnon. Two fire ant species, Solenopsis invicta Buren and Solenopsis geminata (F.), and Pheidole morrissi (Forel) were killed; average percentage of mortality of the five other species was less than 20%. In contrast, all species that were fed the commercial fire ant bait containing hydramethylnon (Amdro) died or were adversely affected. In the field, applications of house fly pupae and eye gnat, Hippelates pusio Loew, pupae dipped in acetone solutions of fenoxycarb significantly reduced population indices of the red imported fire ant, S. invicta, compared with commercial formulations of fenoxycarb (Logic) and hydramethylnon (Amdro). Field observations showed that the pupae of either species can be carried or moved by one or two worker ants. The smooth, hard cuticle of the pupae make them easy to handle and apply with application equipment. The current cost of house fly pupae is considerably more than the cost of a granular carrier, pregel defatted corn grits. However, if mass-production methods reduce this price differential, fly pupae could become an effective and more species-specific fire ant bait carrier.     

Commercial and naturally occurring fly parasitoids (Hymenoptera: Pteromalidae) as biological control agents of stable flies and house flies (Diptera: Muscidae) on California dairies

Filth fly parasites reared by commercial insectaries were released on two dairies (MO, DG) in southern California to determine their effect on populations of house flies, Musca domestica L., and stable flies, Stomoxys calcitrans (L.). Spalangia endius Walker, Muscidifurax raptorellus Kogan and Legner, and Muscidifurax zaraptor Kogan and Legner were released on the MO dairy from 1985 to 1987 in varying quantities. Parasitism by Muscidifurax zaraptor on the MO dairy was significantly higher (P less than 0.05) from the field-collected stable fly (4.4%) and house fly (12.5%) pupae, compared with a control dairy (0.1%, stable fly; 1.3%, house fly). Muscidifurax zaraptor, released from April through October during 1987 on the DG dairy (350,000 per month), was not recovered in a significantly higher proportion from either fly species relative to the corresponding control dairy. No specimens of Muscidifurax raptorellus were recovered from the MO dairy. Parasite treatments had no apparent effect on adult populations of either fly species or on overall parasitism rate of field-collected stable fly (16.8%, MO; 17.2%, DG) and house fly (23.3%, MO; 20.9%, DG) pupae. Spalangia spp. were the predominant parasites recovered from field-collected stable fly and house fly pupae on all four dairies. Sentinel house fly pupae placed in fly-breeding sites on both release dairies were parasitized at a significantly higher rate, as compared with sentinel pupae on control dairies. The generic composition of parasites emerging from sentinel house fly pupae was 20.6% Spalangia spp. and 73.2% Muscidifurax spp., whereas in field-collected house fly pupae, Spalangia spp. and Muscidifurax spp. constituted 74.3 and 19.6% of the parasites, respectively.     

In vitro rearing of the pupal parasitoidBrachymeria intermedia (Hym.: Chalcididae) on artificial diets with and without host components

The pupal parasitoidBrachymeria intermedia (Nees) was reared from the egg to adult stage on artificial media based on commercial meat homogenates for babies (Plasmon®), either with or devoid of host components. Six media containing 80% homogenate and 20% extract ofGalleria mellonella L. pupae were tested first. Two types of homogenates, intended for babies at the beginning of weaning (a) and well on in weaning (b), were utilized. Media were based on beef, veal and chicken meat, 3 on a-homogenates and 3 on b-homogenates. A significantly higher percentage of parasitoids developed as mature larvae on the a- than on the b-homogenate based diets. This was presumably related to the higher protein, carbohydrate, lipid and calorie level of the a- than of the b- homogenates. Diet veal-a produced the best mean adult yield (27.4%). Other two diets based on the veal-a homogenate were then tested. The first, composed of 80% homogenate, 10% host pupal extract, 7% chicken egg yolk, 1.5% yeast extract and 1.5% wheat germ, produced a mean adult yield of 66.7%, similar to that obtained inG. mellonella pupae. On the second medium, devoid of host components and containing 85% veal-a homogenate, 10% chicken egg yolk, 2.5% wheat germ and 2.5% yeast extract, the mean adult yield was 22.5%. In all media, the adults obtained were viable and fecund.     

Expression of cytochrome P-450lpr is developmentally regulated and limited to house fly.

Expression of house fly cytochrome P-450lpr was examined using immunoblotting in male and female adult LPR house flies, mixed sex adult house flies at 12 different ages, larvae, and pupae. P-450lpr was expressed in both male and female adult house flies. P-4501pr was clearly present in all adult stages examined, was barely detectable in pupae, and could not be detected in larvae. Thus, cytochrome P-450lpr is developmentally regulated and present in both sexes of house fly. Expression of cytochrome P-450, immunologically homologous to house fly cytochrome P-4501pr, was examined in other species using immunoblot analysis. Eleven animal species were tested in the orders Diptera, Hymenoptera, Lepidoptera, Orthoptera, Acari, and Rodentia, using microsomes in some species from both induced and noninduced animals or insecticide-resistant and susceptible strains. P-450lpr appears to be restricted to house flies, as none of these species contained cytochrome P-450 that reacted with antiserum to cytochrome P-450lpr.     

Growth response of African catfish,Clarias gariepinus (B.), larvae and fingerlings fed protease‐incorporated diets

African catfish, Clarias gariepinus (B.), is one of the promising freshwater fish species in African aquaculture but the expansion of its farming needs more production of its larvae. The use of live food organisms at first feeding for larvae is still obligatory. That increases the cost of larvae production. Hence, the incorporating of exogenous enzymes especially protease in artificial microdiets may provide affordable alternatives for enhancing the larvae performance. The present study was carried out to evaluate the growth and survival of larvae or fingerlings of African catfish fed artificial diets incorporated with different protease levels. Four artificial diets were formulated and enriched with protease enzyme at levels of 0.0, 750, 1,000, and 1,250 unit/kg diet; after that diets were made into crumbles (100–200 µm diameter). After absorption of the yolk sac, diets were offered to fish larvae (3.6 ± 0.2 mg) in triplicates as a starter feed up to apparent satiation every two hours for 30 days. In another treatment, fish larvae were fed on newly hatched Artemia nauplii (2,500 Artemia/L) as a starter food. In another experiment, African catfish fingerlings (10.1 ± 1.6 g) were fed on the same diets up to satiation twice a day for 2 months. It was noticed that the dietary protease improved larval growth and survival but not as Artemia nauplii did where fish larvae fed on Artemia nauplii showed highest growth and survival followed by those fed a diet enriched with 1,250 unit/kg diet of protease. The mortality of larvae fed protease‐enriched diets as well as the control diet was occurred mostly at the first week reaching its maximum at the third week. The poor growth was observed with fish larvae fed the control diet. Meanwhile, catfish fingerlings fed protease‐enriched diets showed higher growth over those fed the control diet. The larvae survival (11.0%–41.7%) was enhanced by increasing protease levels and it was lower than that of fingerlings (95.6%–100.0%). Furthermore, protein retention and digestibility were significantly improved with protease supplementation over the control diet especially at a level of 1,000 unit/kg diet. As compared with the previous studies, live food should be used in larvae rearing for the first week after that a starter diet enriched with protease at levels of 1,250 unit/kg diet should be used. In case of fish fingerlings, the dry diets should be enriched with 1,100 unit/kg diet to improve diet digestibility and subsequently enhance their growth.     

House fly larvae meal grown on municipal organic waste as a source of protein in poultry diets

House fly larvae (Musca domestica L.) grown in the residues of municipal organic waste were evaluated as a protein concentrate in practical diets for poultry. Seventy-two broiler-type chicks were alloted within 12 groups. Quadruplicate groups each received the following dietary treatments: a control diet containing soya bean meal alone, or two diets in which either 12% of the supplementary protein was provided by fly larvae meal or 9% by fish meal. The chicks were fed during the first four weeks of life with isocaloric and isoproteic diets and the results showed no significant differences in body weight gain (P > 0.05) or food conversion efficiency (P > 0.05), the larvae meal diet being intermediate to the other two.     

Black soldier fly defatted meal as a dietary protein source for broiler chickens: effects on carcass traits,breast meat quality and safety

Finding insect meals as alternative sources of poultry feedstuffs is a recent research topic; therefore, the present study aimed to evaluate the effects of defatted black soldier fly (Hermetia illucens L., HI) larvae meal in broiler chicken diets on the carcass characteristics and meat quality parameters, proximate composition, fatty acid profile and the heavy metal content of the breast meat. Four dietary treatments were designed: a control diet (HI0) and three experimental diets (HI5, HI10 and HI15), corresponding to 50, 100 and 150 g/kg HI inclusion levels, respectively. The inclusion of 50, 100 and 150 g HI meal per kg feed supply 16.56%, 33.01% and 49.63% of required crude protein. The broilers were slaughtered at day 35, the carcasses were weighed and the breast muscles were excised from 16 birds per each feeding group (two birds per replicate pens) and used for meat quality evaluation. Linear and quadratic responses were observed, for increasing HI meal levels, in the live and carcass weights (maximum for HI10). As far as the colour of the breast meat is concerned, redness (a*) showed a linear response, while yellowness (b*) linearly decreased with increasing HI meal levels (minimum for HI15). As the HI larvae meal increased in the diets, the moisture content linearly decreased and the protein content increased. The total saturated fatty acid and total monounsaturated fatty acid proportions rose to the detriment of the polyunsaturated fatty acid fraction. The HI larvae meal, used in the current study, represents a valuable protein source for broiler chickens when included by up to 100 g/kg in their diets, as an improved slaughtering performance was observed without any detrimental effects on meat quality parameters or heavy metal residues in the meat.     

Bacterial protein meal produced on natural gas replacing soybean meal or fish meal in broiler chicken diets

The effects of replacing soybean meal or fish meal with 2, 4 or 6% bacterial protein meal (BPM) on growth performance, ileal digestibility of amino acids and sensory quality of meat, were examined using 630 broiler chickens. Weight gain from 7-32 days of age did not differ significantly among the treatments. Efficiency of feed conversion was increased when BPM replaced soybean meal, and abdominal fat deposition tended to decline. Feed conversion was not affected when BPM replaced fish meal. Amino acid digestibility was unaffected or improved when BPM replaced soybean meal, whereas replacement of fishmeal with BPM resulted in similar digestibility. Sensory quality of fresh thigh meat was similar among treatments, but for freeze-stored chest meat replacement of fish meal with BPM reduced off-odour and off-flavour and increased juiciness. It was concluded that 6% BPM can replace soybean meal or fish meal protein in broiler chicken diets.     

The effect of polyphenols and vitamin E on the antioxidant status and meat quality of broiler chickens exposed to high temperature

The aim of this study was to determine the effect of a polyphenol product (PP) (Proviox) and vitamin E on the antioxidant status and meat quality of broiler chickens exposed to high temperature. The experimental materials comprised 120 ROSS 308 broilers (6 treatments, 10 replications, 2 birds per replication). Dietary supplementation with vitamin E and PP was applied in the following experimental design: group I (negative control) – without supplementation; group II (positive control) – without supplementation; group III – supplementation with 100 mg vitamin E/kg; group IV – 200 mg vitamin E/kg; group V – 100 mg vitamin E/kg and 100 mg PP/kg; group VI – 200 mg PP/kg. In groups II–VI, broiler chickens aged 21–35 d were exposed to increased temperature (34°C for 10 h daily). In chickens exposed to high temperature, dietary supplementation with antioxidants, mostly PP, improved growth performance parameters, including body weight, body weight gain and feed intake until 28 d of age. Vitamin E added to broiler chicken diets at 200 mg/kg and vitamin E combined with PP was most effective in improving the total antioxidant status of birds, enhancing blood antioxidant enzyme activities and increasing vitamin E concentrations in the liver and breast muscles. Broilers fed diets supplemented with 200 mg/kg of vitamin E alone and vitamin E in combination with PP were characterised by a higher percentage content of breast muscles in the carcass. Dietary supplementation with antioxidants improved the water-holding capacity of meat, reduced natural drip loss and increased the crude ash content of meat. The breast muscles of chickens fed diets supplemented with PP had a lower contribution of yellowness. The breast muscles of chickens receiving diets with 100 mg vitamin E/kg(group III) and diets supplemented with PP (groups V and VI) were characterised by the highest concentrations of polyunsaturated fatty acids. The PP can be a valuable component of diets for broiler chickens exposed to high temperature.     

Susceptibility of black soldier fly (Diptera: Stratiomyidae) larvae and adults to four insecticides

Dosage-mortality regressions were determined for black soldier fly, Hermetia illucens (L.), larvae fed cyromazine or pyriproxifen treated media. Cyromazine LC50 for larvae dying before becoming prepupae ranged from 0.25 to 0.28 ppm with dosage-mortality regression slopes between 5.79 and 12.04. Cyromazine LC50s for larvae dying before emergence ranged from 0.13 to 0.19 ppm with dosage-mortality regression slopes between 3.94 and 7.69. Pyriproxifen dosage-mortality regressions were not generated for larvae failing to become prepupae since <32% mortality was recorded at the highest concentration of 1,857 ppm. LC50s for larvae failing to become adults ranged from 0.10 to 0.12 ppm with dosage mortality-regression slopes between 1.67 and 2.32. Lambda-cyhalothrin and permethrin dosage-mortality regressions were determined for wild adult black soldier flies and house flies, Musca domestica L., and for susceptible house flies. Our results indicate that the wild house fly, unlike the black soldier fly, population was highly resistant to each of these pyrethroids. Regression slopes for black soldier flies exposed to lambda-cyhalothrin were twice as steep as those determined for the wild house fly strain. Accordingly, LC50s for the black soldier fly and susceptible house fly were 10- to 30-fold lower than those determined for wild house flies. The differential sensitivity between wild black soldier flies and house flies might be due to behavioral differences. Adult house flies usually remain in animal facilities with the possibility of every adult receiving pesticide exposure, while black soldier fly adults are typically present only during emergence and oviposition thereby limiting their exposure.