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1.
以‘垂丝海棠’(Malus halliana)和‘平邑甜茶’(Malus hupehensis)为基砧,分别嫁接品种‘烟富6号’和‘长富2号’接穗,测定4种砧穗组合的嫁接亲和性、接穗生长量、光合与荧光参数及叶绿素含量(SPAD),并用主成分分析法综合评价砧穗组合的优劣,探讨不同苹果砧穗组合嫁接苗的生长及光合特性,为西北盐碱地选择适宜的苹果砧木提供理论依据。结果表明:(1)4种砧穗组合中‘垂丝海棠/烟富6号’的上下口粗度比最接近1,嫁接亲和性最好。(2)整个生长期内,以‘垂丝海棠’为基砧的2个组合嫁接苗的生长量、净光合速率(Pn)、最大荧光(Fm)、PSⅡ最大光能转化率(Fv/Fm)均显著大于‘平邑甜茶’为基砧的组合,但其胞间CO2浓度(Ci)及初始荧光(F0)显著低于‘平邑甜茶’为基砧的组合;光化学猝灭系数(qP)在4种砧穗组合中无显著差异。(3)在8月份光照强度较高时,‘垂丝海棠/烟富6号’ 嫁接苗的气孔导度(Gs)高于其他砧穗组合;以‘垂丝海棠’为基砧的2个组合嫁接苗叶片的蒸腾速率(Tr)和相对叶绿素含量(SPAD)显著高于‘平邑甜茶’ 基砧组合。(4)根据主成分分析对各项指标进行综合评价,按照4个砧穗组合的综合得分由高到低依次为:‘垂丝海棠/烟富6号’、‘垂丝海棠/长富2号’、‘平邑甜茶/长富2号’、‘平邑甜茶/烟富6号’。研究发现,基砧‘垂丝海棠’的适应性优于‘平邑甜茶’,且‘垂丝海棠/烟富6号’砧穗组合的嫁接亲和性高,长势强,光合能力优,为甘肃中部地区适宜的砧穗组合。  相似文献   

2.
以2年生苹果矮化砧木M9 T337为试材,采用盆栽试验法,设置浇灌清水(CK)和盐碱胁迫(0.1 mol/L NaCl+NaHCO3溶液)+ 喷施5种浓度的H2O2 [0(T1)、0.2 mmol/L(T2)、0.4 mmol/L(T3)、0.6 mmol/L(T4)、0.8 mmol/L(T5)] 处理,测定各处理叶片叶绿素含量、光合气体交换参数、渗透调节物质含量、抗氧化酶活性和细胞膜透性,并利用相关性与主成分分析进行综合评价,以探讨外源过氧化氢(H2O2)增强其盐碱耐性的生理机制。结果表明:(1)随着盐碱胁迫(T1)的时间延长,M9 T337幼苗叶片叶绿素a(Chl a)含量、叶绿素b (Chl b)含量、叶绿素总量(Chl t)、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、可溶性蛋白(SP)含量均呈逐渐下降趋势;胞间CO2浓度(Ci)、可溶性总糖(TSS)含量、脯氨酸(Pro)含量、过氧化氢酶(CAT)活性、抗坏血酸过氧化物酶(APX)活性、相对电导率(REC)、丙二醛(MDA)含量均呈上升趋势;超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性均呈先升后降趋势。(2)与CK相比,盐碱胁迫+外源H2O2(T2- T5)处理后M9 T337幼苗叶片各指标均呈现不同幅度变化,且存在明显浓度效应,并以T3(0.4 mmol/L H2O2)处理叶片的Chl a、Chl b、Chl t、SP和Gs降幅最小,Ci、REC、MDA升幅最小,TSS、Pro、APX升幅最大。(3)M9 T337幼苗叶片PnTrGs、Chl a、Chl b、Chl t、SP、SOD、POD呈显著正相关,与Ci、MDA、CAT、APX、REC呈显著负相关。(4)综合评价表明,各处理对M9 T337幼苗叶片生理特性的效应依次为:CK>T3>T4>T2>T5>T1。研究发现,叶面喷施适宜浓度H2O2可有效改善盐碱胁迫下M9 T337幼苗光合能力,显著提高抗氧化酶活性和渗透调节物质的含量,降低细胞膜透性,从而达到缓解盐碱胁迫的作用,并以0.4 mmol/L H2O2处理效果最佳。  相似文献   

3.
以‘绿博6号’黄瓜幼苗为试材,采用营养液培法研究不同浓度外源硅(0、0.5、1.0、1.5和2.0 mmol/L)对肉桂酸(cinnamic acid,CA)模拟自毒胁迫(3.0 mmol/L CA)下黄瓜幼苗生长、光合参数和叶绿素荧光参数的影响。结果表明:(1)自毒胁迫显著抑制了黄瓜幼苗的生长、根系形态建成和生物量的积累,显著降低了净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和叶绿素(Chl a、Chl b和Chl t)含量,并显著降低了叶片中PSⅡ的电子传递速率(ETR)、PSⅡ实际光化学效率[Y(Ⅱ)]和光化学淬灭系数(qP)。(2)添加适宜浓度外源硅可有效缓解自毒胁迫对黄瓜幼苗生长的影响,并提高其PnGsTr和叶绿素含量,在一定程度上维持叶片光合系统的稳定。(3)添加适宜浓度外源硅能使自毒胁迫下黄瓜叶片的Fv/Fm、ETR、Y(Ⅱ)qP显著升高,而非光化学淬灭系数(NPQ)则显著下降。研究发现,添加适宜浓度外源硅能提高自毒胁迫下黄瓜幼苗的叶绿素含量、Fv/Fm、ETR、Y(Ⅱ)qP,使光合机构趋于稳定,抑制Pn的下降,缓解自毒胁迫对光合系统的损伤,从而增强黄瓜幼苗对自毒胁迫的抗性,并以1.0 mmol/L外源硅处理的效果最佳。  相似文献   

4.
干旱胁迫下4种常用植物幼苗的光合和荧光特性综合评价   总被引:5,自引:0,他引:5  
卢广超  许建新  薛立  刘斌  李诗刚  邵怡若 《生态学报》2013,33(24):7872-7881
老鸭嘴,山毛豆,假连翘和葛藤是常见的矿山植被恢复植物。采用盆栽控制土壤水分的方法,测定了这 4 种幼苗叶片的光合和荧光指标,以期为矿山植被的恢复植物筛选提供依据。结果表明(1)干旱胁迫处理期间,4 种幼苗叶片的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和胞间CO2 浓度(Ci)持续下降,复水8 d后Pn均显著增加,老鸭嘴和山毛豆的GsTr恢复到对照水平,山毛豆和葛藤的Ci恢复到对照水平;假连翘幼苗叶片的气孔限制值(Ls)随干旱胁迫时间的延长而逐渐增加,其他3 种幼苗略降后增加,复水8 d后均恢复到对照水平。(2)干旱胁迫处理期间,4 种幼苗叶片的photosystemⅡ(PSⅡ)光能捕获效率(Fv’/Fm’)、实际光化学量子效率(Yield)和表观电子传递速率(ETR)持续降低,8 d时显著小于对照。复水8 d后,Fv’/Fm’小幅波动,Yield和ETR有所恢复,其中葛藤的Yield和山毛豆的ETR恢复到对照水平;4种幼苗叶片的非化学淬灭(NPQ)持续上升,在第8天显著大于对照,复水8 d后,均持续下降,其中葛藤恢复到对照水平。(3)对光合和荧光指标进行主成分分析表明,干旱胁迫下4 种幼苗的抗旱性能大小顺序为山毛豆 > 老鸭嘴 > 假连翘 > 葛藤。  相似文献   

5.
红芽芋驯化苗对盐胁迫的光合及生理响应   总被引:1,自引:0,他引:1  
洪森荣  尹明华 《西北植物学报》2013,33(12):2499-2506
为探讨江西铅山红芽芋的耐盐机制,以其组培移栽驯化苗为材料,研究了盐胁迫对其生物量积累、光合特性、荧光特性等抗逆生理特性的影响。结果表明:(1)红芽芋幼苗生物量和根冠比在低盐胁迫下(50 mmol·L-1)得到显著促进,而在高盐胁迫下(100~250 mmol·L-1)受到显著抑制。(2)低盐胁迫幼苗的净光合速率(Pn)、气孔导度(Gs)、气孔限制值(Ls)、水分利用效率(WUE)和瞬时羧化效率(CUE)比对照(0 mmol·L-1)显著增加,细胞间CO2浓度(Ci)比对照显著下降,蒸腾速率(Tr)与对照无显著差异;高盐胁迫幼苗的PnLsWUECUEGs较对照显著下降,Ci比对照显著增加。(3)低盐胁迫幼苗的最大荧光(Fm)、PSⅡ潜在光化学效率(Fv/F0)和光化学猝灭系数(qP)比对照显著增加,初始荧光(F0)较对照显著下降,PSⅡ最大光化学效率(Fv/Fm)、PSⅡ实际光化学效率(ΦPSⅡ)、开放的PSⅡ反应中心捕获激发能效率(Fv′/Fm′)和非光化学猝灭系数(NPQ)与对照无显著差异;而高盐胁迫幼苗的F0FmFv/FmFv/F0、ΦPSⅡFv′/Fm′和qP均较对照显著下降,NPQ比对照显著增加。(4)各盐胁迫幼苗叶片的可溶性蛋白含量以及过氧化物酶、超氧化物歧化酶和过氧化氢酶活性与对照相比先升后降,并以低盐下最高;可溶性总糖和脯氨酸含量均比对照显著增加;丙二醛含量和质膜透性相对值在低盐胁迫下无显著变化,而在高盐下显著增加;叶绿素含量和根系活力在低盐胁迫下无显著变化,而在高盐胁迫后开始显著下降。研究发现,江西铅山红芽芋移栽驯化苗的耐盐阈值为 50 mmol·L-1,其能够诱导提高叶片可溶性蛋白含量和主要保护酶活性,稳定质膜透性、叶绿素含量和根系活力,增加PSⅡ潜在光化学效率,提高PSⅡ的电子传递活性,维持PSⅡ实际光化学效率,有效启动非辐射热能量耗散机制来保护了光合机构,最终提高净光合速率,增加生物量。  相似文献   

6.
谷从璟  周健  马丹炜  黄素  孟巧巧 《广西植物》2019,39(10):1317-1326
以蚕豆(Vicia faba)为受体,采用盆栽试验评价了入侵植物土荆芥(Chenopodium ambrosioides)挥发油及其两个主要成分α-萜品烯和对伞花素对受体光合特性的影响。结果表明:土荆芥挥发油及其两个主要成分不同程度地影响了蚕豆叶片的特性。挥发油处理显著降低了净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)和叶绿素含量,但增加了胞间CO2浓度(Ci),这种效应表现为剂量和时间双重效应,高剂量挥发油处理的这种效应是不可逆的; 与对照相比,α-萜品烯处理组的Pn、Fv/FmΦPSⅡ降低,CiGsTr上升,停止处理后,各参数均趋于对照水平; 整体来看,对伞花素对蚕豆幼苗的光合特性影响不大。上述研究结果说明,土荆芥化感胁迫对受体光合特性的影响是诸多化感物质协同作用的结果,并非由单一组分决定。  相似文献   

7.
为了揭示黄腐酸对干旱胁迫下燕麦光合及其抗氧化酶活性的影响机理,该研究选用燕麦品种‘燕科2号’为材料,采用盆栽试验,以正常供水(田间持水量的75%)为对照(CK),设干旱胁迫处理(田间持水量的45%,D0)、D0 + 喷施不同浓度黄腐酸(0、200、400、600、800、1 000 mg/L)处理(D1~D5),测定各处理燕麦干鲜重、光合性能及其抗氧化酶活性。结果表明:(1)与CK相比,干旱胁迫下燕麦幼苗地上部鲜重和干重、叶片光合色素含量、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)均显著降低,并导致叶片Fv/FmqP、ETR和ΦPSⅡ显著下降,使叶片抗氧化酶 SOD、POD、CAT活性分别显著提高25.68%、19.98%和7.29%。(2)与D0相比,D0 +喷施600 mg/L黄腐酸后,燕麦幼苗地上部鲜重和干重分别显著提高了28.59%和39.13%,叶片叶绿素a、叶绿素a+b、类胡萝卜素和PnGsTrFv/Fm、ΦPSⅡ、ETR分别显著增加了25.17%、21.03%、47.37%和74.38%、26.47%、43.34%及6.49%、69.57%、70.71%,CiFo、NPQ分别显著降低了19.52%和13.32%、43.75%。(3)干旱胁迫下喷施不同浓度的黄腐酸均使幼苗叶片中SOD、POD、CAT活性较D0处理显著增加,其中喷施600 mg/L黄腐酸的叶片SOD、POD、CAT活性最高,分别较D0处理显著增加了12.19%、76.57%和55.26%。研究认为,叶面喷施适宜浓度黄腐酸能够显著提高干旱胁迫下燕麦幼苗的光合作用及其抗氧化能力,缓解干旱对燕麦幼苗的伤害,进而促进燕麦幼苗的生长,且以叶面喷施600 mg/L黄腐酸效果最佳。  相似文献   

8.
为了探讨低温弱光下水杨酸(SA)对黄瓜光合功能的调控作用,以‘津优3号’黄瓜幼苗为试材,叶面喷施不同浓度的SA溶液,研究低温弱光下黄瓜幼苗气体交换参数、光化学效率、MDA含量及抗氧化酶活性的变化.结果表明:低温弱光胁迫使黄瓜幼苗叶片的光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、PSⅡ光下实际光化学效率(ΦPSⅡ)及暗下最大光化学效率(Fv/Fm)明显降低,胞间CO2浓度(Ci)显著升高,说明低温弱光下黄瓜幼苗Pn下降的主要原因是非气孔限制;低温弱光还可引起黄瓜幼苗丙二醛(MDA)含量增加,超氧化物歧化酶(SOD)活性升高,过氧化氢酶(CAT)活性降低,过氧化物酶(POD)活性先升高后降低.而胁迫前用0.5~2.5 mmol·L-1 SA预处理幼苗,其叶片的Pn、Gs、Tr、ΦPSⅡ、Fv/Fm及SOD、POD和CAT活性与CK(水预处理)相比均有不同程度的提高,Ci和MDA含量有所降低.表明SA可有效调控低温弱光下黄瓜幼苗叶片的光合功能,提高其低温弱光耐性,其适宜浓度为1 mmol·L-1.  相似文献   

9.
研究外源GA3对盐胁迫下黄瓜种子萌发和幼苗生长的影响。结果表明,添加不同质量浓度GA3的各处理,其发芽率、发芽势和发芽指数均显著高于NaCl胁迫处理,其中以100 mg/L GA3处理的发芽势、发芽率和发芽指数最高,幼苗的叶面积、根长、根冠比也最大,同时叶片中叶绿素含量最高,幼苗叶片的光合速率(Pn)、气孔导度(Gs)、胞间CO2摩尔分数(Ci)及蒸腾速率(Tr)等均达到最大;而当赤霉素的质量浓度为50 mg/L时,叶片中的POD活性为2 005 U/(g·min),达最大值。  相似文献   

10.
Response of net photosynthetic rate (P N), stomatal conductance (g s), intercellular CO2 concentration (c i), and photosynthetic efficiency (Fv/Fm) of photosystem 2 (PS2) was assessed in Eucalyptus cladocalyx grown for long duration at 800 (C800) or 380 (C380) μmol mol-1 CO2 concentration under sufficient water supply or under water stress. The well-watered plants at C800 showed a 2.2 fold enhancement of P N without any change in g s. Under both C800 and C380, water stress decreased P N and g s significantly without any substantial reduction of c i, suggesting that both stomatal and non-stomatal factors regulated P N. However, the photosynthetic efficiency of PS2 was not altered. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

11.
Scirtothrips perseae Nakahara was discovered attacking avocados in California, USA, in 1996. Host plant surveys in California indicated that S. perseae has a highly restricted host range with larvae being found only on avocados, while adults were collected from 11 different plant species. As part of a management program for this pest, a “classical” biological control program was initiated and foreign exploration was conducted to delineate the home range of S. perseae, to survey for associated natural enemies and inventory other species of phytophagous thrips on avocados grown in Mexico, Guatemala, Costa Rica, the Dominican Republic, Trinidad, and Brazil. Foreign exploration efforts indicate that S. perseae occurs on avocados grown at high altitudes (>1500 m) from Uruapan in Mexico south to areas around Guatemala City in Guatemala. In Costa Rica, S. perseae is replaced by an undescribed congener as the dominant phytophagous thrips on avocados grown at high altitudes (>1300 m). No species of Scirtothrips were found on avocados in the Dominican Republic, Trinidad, or Brazil. In total, 2136 phytophagous thrips were collected and identified, representing over 47 identified species from at least 19 genera. The significance of these species records is discussed. Of collected material 4% were potential thrips biological control agents. Natural enemies were dominated by six genera of predatory thrips (Aeolothrips, Aleurodothrips, Franklinothrips, Leptothrips, Scolothrips, and Karnyothrips). One genus each of parasitoid (Ceranisus) and predatory mite (Balaustium) were found. Based on the results of our sampling techniques, prospects for the importation of thrips natural enemies for use in a “classical” biological control program in California against S. perseae are not promising.  相似文献   

12.
13.
Parsimony analyses of the internal transcribed spacer regions of nuclear ribosomal DNA (ITS 1 & ITS 2) for 38 taxa sampled from the Phebalium group (Rutaceae: Boronieae) and two outgroups confirm that, with the exception of Phebalium sensu stricto and Rhadinothamnus, six of the currently recognised genera within the group are monophyletic. The data indicate that Phebaliums. str. is paraphyletic with respect to Microcybe, and Rhadinothamnus is paraphyletic with respect to Chorilaena. Rhadinothamnus and Chorilaena together are the sister group to Nematolepis. Drummondita, included as an outgroup taxon, clustered within the ingroup as sister to Muiriantha and related to Asterolasia.The phylogeny suggests that the evolution of major clades within a number of these genera (e.g. Phebalium) relates to vicariance events between eastern and south-western Australia. Leionema is an eastern genus, with the most basal taxon being the morphologically distinct Leionema ellipticum from northern Queensland. Leionema also includes one species from New Zealand, but this species (as with some others) proved difficult to sequence and its phylogenetic position remains unknown. Taxonomic changes at the generic level are recommended.The authors wish to thank Paul G.Wilson, PERTH, for advice and discussion, and Paul Forster, BRI, for collecting and providing material of Leionema ellipticum. The project was supported by a Melbourne University Postgraduate Award (to BM), the Australian Biological Resources Study (ABRS), Australian Systematic Botany Society and Wolf Den (Australia) Investments.  相似文献   

14.
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.
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15.
Komárek has recently reviewed the various species assigned to the green algal genusNeochloris Starr (Chlorococcales, Chlorococcaceae) and removed those with uninucleate vegetative cells to a new genus,Ettlia. Watanabe & Floyd, unaware ofKomárek's work, also reviewed the species ofNeochloris and distributed them among three genera—Neochloris, Chlorococcopsis gen. nov., andParietochloris gen. nov.—on the basis of details of the covering of the zoospore and the arrangement of the basal bodies of the flagellar apparatus. This paper reconciles these two treatments and makes additional recommendations at the ranks of genus, family, order, and class.  相似文献   

16.
seventeen new species and combinations are proposed in the generaChondrorhyncha, Cischweinfia, Cochlioda, Eloyella, Encyclia, Kefersteinia, Koellensteinia, Macroclinium, Rodriguezia, Solenidiopsis, andStenia. All new species are illustrated. A key is provided for 2-flowered species ofMacroclinium, PeruvianSigmatostalix, and PeruvianStenia. Solenidium (Solenidiopsis) peruvianum Schltr. is lectotypified.  相似文献   

17.
郭英兰 《菌物学报》1991,10(Z1):119-128
本文报导作者采自安徽枯牛降自然保护区的18种丝孢菌,分属于5个属,其中有3个新种:牛皮冻生尾孢(Cercospora paederiicola),山鸡椒假尾饱(Pseudocercospora litseae-cubebae),鸡血藤生假尾孢(P. millettiicola)和2个中国新纪录。文中对新种进行了描述及绘图,新记录种作了简要说明。研究的标本保存在中国科学院微生物研究所真菌标本室(HMAS)。  相似文献   

18.
Little is known about how tropical land-use systems contribute to the conservation of functionally important insect groups, including dung beetles. In a study at the margin of Lore Lindu National Park (a biodiversity hotspot in Central Sulawesi, Indonesia) dung-beetle communities were sampled in natural forest, young secondary forest, agroforestry systems (cacao plantations with shade trees) and annual cultures (maize fields), each with four replicates (n = 16 sites). At each site we used 10 pitfall traps, baited with cattle dung, along a 100 m transect for six 3-day periods. The number of trapped specimens and species richness at the natural forest sites was higher than in all land-use systems, which did not significantly differ. Each land-use system contained, on average, 75% of the species richness of the natural forest, thereby indicating their importance for conservation. However, a two-dimensional scaling plot based on NESS indices (m = 6) indicated distinct dung beetle communities for both forest types, while agroforestry systems and annual cultures exhibited a pronounced overlap. Mean body size of dung beetles was not significantly influenced by land-use intensity. Five of the six most abundant dung beetle species were recorded in all habitats, whereas the abundance of five other species was significantly related to habitat type. Mean local abundance and number of occupied sites were closely correlated, further indicating little habitat specialisation. The low dung beetle diversity (total of 18 recorded species) may be due to the absence of larger mammals in Sulawesi during historical times, even though Sulawesi is the largest island of Wallacea. In conclusion, the dung beetle fauna of the lower montane forest zone in Central Sulawesi appears to be relatively robust to man-made habitat changes and the majority of species did not exhibit strong habitat preferences.  相似文献   

19.
The genusKarschia, in the earlier sense, including saprophytes and parasites on lichens, has been thought to be a non-lichenized parallel genus of the lichen genusBuellia. Modern workers included it on the one hand inBuellia, on the other hand combined it with bitunicate ascomycetes. It is now proved thatKarschia is heterogeneous and contains but superficially similar members both of the genusBuellia of theLecanorales and of typical or masked bitunicateAscomycetes. Therefore, it can not be regarded as a link betweenLecanorales andDothideales. The type species ofKarschia belongs to theDothideales.
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