镰叶西番莲的化学成分

从镰叶西番莲（Passiflora wilsonii）的腾茎中分离到6个化合物,经理化性质和波谱分析,分别确定为：glut-5-en-3β-ol（I）、maslinic acid(Ⅱ）,friedelin(Ⅲ）,ergosterol epidioxide(Ⅴ）和β-谷甾醇（Ⅳ）。以上化合物均为首次从镰叶西番莲的腾茎中分离得到。     

海洋放线菌Streptomyces variabilisstrain 6-1次级代谢产物的研究

目的：对来自海洋软珊瑚的链霉菌6-1（Streptomyces variabilisstrain6-1）进行次级代谢产物的分离和鉴定,寻找具有生物活性的化合物,为人类健康服务。方法：采用液体培养基对分自海洋软珊瑚Scleronephthya sp中的链霉菌6．1（Streptomyces vafiabilisstrain6-1）进行发酵培养,用乙酸乙酯对发酵液进行萃取;采用半制备高效液相色谱（semi-preparative HPLC）分离方法对乙酸乙酯萃取物进行分离纯化,得到单体化合物;运用电喷雾质谱（ESI—MS）、核磁共氢振（1HNMR）、核磁共振碳谱（13C NMR）和物理性质对所得单体化合物进行结构鉴定。结果：从海洋链霉菌6-1（strain6-1）发酵液的乙酸乙酯萃取物中分离得到3个单体化合物,分别鉴定为：7,4’-二羟基异黄酮（1）、5,7,4’-三羟基异黄酮（2）和丁烯酸内酯-I（3）。结论：丁烯酸内酯．I是从链霉菌属首次分离得到,化合物1和2均是从Streptomyces variabilis中首次分离得到;变异链霉菌6-1（Stmptomyces variabilis strain6-1）可以作为活性化合物3（丁烯酸内酯-I）的重要来源。     

蒙椴树皮化学成分的研究

为研究蒙椴（Tilia mongolica Maxim.）树皮的化学成分,通过溶剂萃取、硅胶柱色谱、重结晶分离纯化,从蒙椴树皮中分离得到7个化合物,根据其红外光谱、质谱和核磁共振谱数据鉴定为β-香树脂醇乙酸酯（1）,计曼尼醇乙酸酯（2）,羽扇豆醇（3）,二十四碳酸（4）,三棕榈酸甘油酯（5）,β-谷甾醇（6）,胡萝卜苷（7）。其中化合物1-5、7均为首次从椴树属植物中分离得到,化合物6为首次从该植物中分离得到。     

朗德鹅胆汁的化学成分及金属蛋白酶抑制活性研究

应用多种色谱技术进行分离纯化,从朗德鹅胆汁85％乙醇提取物中分离得到6个化合物。经理化性质和光谱数据分析鉴定为苯乙酸（1）、鹅去氧胆酸（2）、鹅去氧胆酸乙酯（3）、棕榈酸-α-单甘油酯（4）、顺-6-十八碳烯酸（5）、（4E）-2-[2＇-hydroxyhexadecanoylamino]-4-octadecane-1,3-diol（6）。化合物1、3、4和6为首次从该属动物胆汁中分得,其中化合物6为首次从陆生动物胆汁中分得的一种神经酰胺类成分。首次对化合物2、4和5进行抑制金属蛋白酶活性的实验,评价了三个化合物的生物活性。     

多根硬皮马勃中子实体的化学成分

从多根硬皮马勃（Scleroderma polyrhizum Pers．）子实体中分离得到了3个含氮化合物,根据化学和光谱数据,它们的化学结构分别确定为：N,N-dimethylphenylalanine（1）,2-N,N,N-trimethyl-phenylalanine（2）,2-trimethyl-ammonio-3-（3-indolyl）propionate（3）。上述含氮化合物均首次从多根硬皮马勃中分离得到,其中化合物2首次从高等真菌中分离得到。     

顶杯衣（Acroscyphus sphaerophoroides）的化学成分研究

对采自云南丽江老君山的顶杯衣（AcroscyphussphaerophoroidesL6v．）的化学成分进行了研究,从中分离得到9个化合物,利用谱学分析方法与文献对照,确定了它们的化学结构。分别鉴定为：甲基茶渍酸（methylle—canorate,I）、茶渍酸（1ecanoricacid,Ⅱ）、三苔色酸（gyrophoricacid,Ⅲ）、地衣酚（orcinol,Ⅳ）、苔色酸甲酯（Methylorsellinate,V）、天精（skyrin,VI）、内赤藓醇（meso—erythritol,Ⅶ）、伏勒米醇（volemitol,Ⅷ）及棕榈酸（palmit—icacid,Ⅸ）。其中化合物I、Ⅱ、Ⅳ、V为首次从顶杯衣中分离得到。化合物Ⅲ和Ⅵ是顶杯衣的主要特征性成分。     

偏翅唐松草化学成分的研究

从偏翅唐松草（Thalictrum delavayi Franch.)的乙醇提取物的氯仿部分分离到9个化合物（1-9）。其中化合物1和2为新化合物,分别为5-hydro-N-methylcorydalidine(1)和1-（4-methoxybenzyl)-2-N-methyl-6-hydroxyl-5,7-dimethoxy-isoquinoline(2)。化合物7为首次从唐松草属中分离到,它们的结构经波谱方法（包括HMQC和HMBC）得到鉴定。     

中国沉香精油化学成分研究

中国沉香[原植物为Aquilaria sinensis(Lour.)Gilg]的精油经硅胶,Sephedex-20柱层析分离,得到两个新的倍半萜 化合物,分别命名为呋喃白木香醛和呋喃白木香醇,经波谱分析（IR,NMR,MS）相应的衍生物呋喃白木香酸X－衍射解析,确定了它们的结构为（I）和（II）,同时,也分离得到4个已知化合物,它们分别是白木香醛（Ⅲ）,白木香酸（IV）,二氢卡拉酮（V）,β-－沉香呋喃（VI）。     

中国沉香精油化学成分研究

中国沉香[原植物为Aquilaria sinensis(Lour.)Gilg]的精油经硅胶,Sephedex-20柱层析分离,得到两个新的倍半萜 化合物,分别命名为呋喃白木香醛和呋喃白木香醇,经波谱分析（IR,NMR,MS）相应的衍生物呋喃白木香酸X－衍射解析,确定了它们的结构为（I）和（II）,同时,也分离得到4个已知化合物,它们分别是白木香醛（Ⅲ）,白木香酸（IV）,二氢卡拉酮（V）,β-－沉香呋喃（VI）。     

印楝植物内生真菌Phomopsis sp.的代谢产物研究

从印楝植物内生真菌Phomopsis sp．的菌丝体提取物中分离得到4个化合物,通过波谱技术分别鉴定为水苏碱（1）、甲基-β-D-葡萄糖苷（2）、过氧化麦角甾醇（3）、腺嘌呤核苷（4）,这些化合物均为首次从该属真菌中分离得到。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Synthesis and antimicrobial studies of hydrazone derivatives of 4-[3-(2,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid and 4-[3-(3,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid

Microbial resistance to antibiotics is an unresolved global concern, which needs urgent and coordinated action. One of the guidelines of the Centers for Disease Control and Preventions (CDC) to combat antibiotic resistance is the development of new antibiotics to treat drug-resistant bacteria. In our effort to find new antibiotics, we report the synthesis and antimicrobial studies of 30 new pyrazole derivatives. These novel molecules have been synthesized by using readily available starting materials and benign reaction conditions. Some of these molecules have shown activity with MIC values as low as 0.78?µg/mL against four bacterial strains; Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Acinetobacter baumannii. Furthermore, active molecules are non-toxic to mammalian cell line.

     

Novel compounds with potent CDK9 inhibitory activity for the treatment of myeloma

Cyclin-dependent kinases (CDKs) and Polo-like kinases (PLKs) play key role in the regulation of the cell cycle. The aim of our study was originally the further development of our recently discovered polo-like kinase 1 (PLK1) inhibitors. A series of new 2,4-disubstituted pyrimidine derivatives were synthesized around the original hit, but their PLK1 inhibitory activity was very poor. However the novel compounds showed nanomolar CDK9 inhibitory activity and very good antiproliferative effect on multiple myeloma cell lines (RPMI-8226).