Predation by Halmus chalybeus (Coleoptera: Coccinellidae) on Ceroplastes destructor and C. sinensis (Hemiptera: Coccidae: Ceroplastinae) Infesting Citrus in Northland, New Zealand

Predation by Halmus chalybeus (steelblue ladybird) on two species of wax scale was studied on citrus orchards in Northland, New Zealand. Field experiments using an exclusion technique of enclosing citrus branches in bags, found that larval and adult H. chalybeus preyed on first and second instars of both Ceroplastes destructor (white wax scale) and C. sinensis (Chinese wax scale), but not third instar C. destructor. Third instar C. sinensis and adults of both species were not tested but are rarely, if ever, attacked by H. chalybeus. The sampling of scale and ladybird populations and field experiments showed that few scales of either species survived past the second instar stage where H. chalybeus was numerous. The feeding rate of H. chalybeus on settled first and second instar scales was estimated inside bagged branches. Adults consumed on average 15.6 C. destructor and 13.3 C. sinensis per day per ladybird, while larvae ate 9.7 C. destructor per day. These feeding rates accounted for the experimental reduction of scale populations. H. chalybeus is a useful natural enemy for the control of C. destructor and C. sinensis when its activity is not disrupted by pesticides.     

力竭追赶训练对两种鲤科鱼类呼吸循环系统参数和力竭运动后代谢特征的影响

为了考察力竭追赶训练对中华倒刺鲃(Spinibarbus sinensis)和岩原鲤(Procypris rabaudi)幼鱼呼吸循环系统和力竭运动后代谢特征的影响, 在(25±0.5)℃条件下, 将2种实验鱼各60尾 [体重分别为(28.36±0.08) g和(19.53±0.13) g]随机等分成对照组和训练组, 训练组进行1次/d共21d的力竭追赶训练。随后测定各组实验鱼的心脏和鳃指数、血液指标以及力竭运动后过量耗氧(Excess post-exercise oxygen consumption, EPOC)。结果发现: 2种鱼心脏指数、鳃指数、血红蛋白含量和红细胞数目在各自对照组和训练组之间都没有显著性差异; 岩原鲤对照组的鳃指数显著低于中华倒刺鲃对照组(P<0.05), 但心脏指数、血红蛋白含量和红细胞数目与中华倒刺鲃对照组无显著性差异。中华倒刺鲃训练组的运动前代谢率、运动代谢峰值、峰值比率、代谢恢复速率与其对照组没有显著差异, 但运动后恢复时间和过量耗氧显著大于对照组(P<0.05); 岩原鲤训练组的运动前代谢率显著低于其对照组(P<0.05), 但运动代谢峰值、峰值比率、运动后恢复时间、过量耗氧、代谢恢复速率与其对照组没有显著性差异。岩原鲤对照组的运动前代谢率、运动代谢峰值、过量耗氧和代谢恢复速率分别显著低于中华倒刺鲃对照组(P<0.05), 但峰值比率和运动后恢复时间与中华倒刺鲃对照组无显著性差异。研究表明: (1)力竭追赶训练对中华倒刺鲃和岩原鲤幼鱼的呼吸和循环系统参数没有产生显著性影响; (2)力竭追赶训练显著提高了中华倒刺鲃幼鱼的无氧代谢能力; (3)中华倒刺鲃幼鱼力竭运动前后代谢率显著高于岩原鲤幼鱼, 这可能与其较为活跃的习性有关。     

四种绒螯蟹分子分类与系统发育

利用PCR技术,扩增了中华绒螯解、狭额绒螯蟹线粒体16SrDNA片段,经测序,与GenBank数据库中的日本绒螯解16SrDNA同源序列进行比较。结果显示,在长为376bp的16SrDNA同源序列中有33个多态性核革酸位点（8．78％）,其中种间多态性核苷酸位点28个（7．45％）,种间差异远大于种内差异。引入方蟹科其它近缘种类厚纹蟹、相手蟹和张口蟹的16SrDNA同源序列与上述4种绒螯蟹比较分析,MP法和NJ法构建的分子系统树表明：中华绒螯蟹与日本绒螯蟹亲缘关系最近,首先聚在一起,然后与台湾绒螯蟹聚为一支,狭额绒螯蟹则为相对独立的一支,且进化速度大于前3种绒螯蟹,但最后与前者仍聚在同一组,狭额绒螯蟹只是绒螯蟹属系统进化中的一个侧支,故本研究结果不支持Sakai（1983）和Guo等（1997）把狭额绒螯蟹和台湾绒螯蟹各自立为新属的观点。     

AGENTS INTRODUCED INTO AUSTRALIA FOR THE BIOLOGICAL CONTROL OF GASCARDIA DESTRUCTOR (NEWSTEAD) (HEMIPTERA: COCCIDAE)

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Six species of parasitic Hymenoptera and 1 predatory Lepidoptera from South Africa, and 1 Hymenoptera from New Zealand were liberated for biological control of white wax scale Gascardia destructor (Newstead) in Australia. Four of the South African Hymenoptera have become established and at least 2, Anicetus communis (Annecke) and Paraceraptrocerus nyasicus (Compere), are contributing to control of G. destructor in eastern Australia.     

角蜡蚧和日本龟蜡蚧蜡泌物的超微结构及化学成分分析

采用扫描电镜和气相色谱/质谱联用技术研究了角蜡Ceroplastes ceriferus (Fabricius) 和日本龟蜡蚧C. japonicus Green蜡泌物的超微结构及化学成分。结果表明,这2种蜡蚧分泌蜡质和形成蜡壳的过程基本相似。在1、2龄期分泌的蜡质为“干蜡”,蜡壳为星芒状,虫体周缘的蜡芒均为2大节,与其2个龄期发育相对应,每一个大节又分为若干小节。同时,虫体背面中央蜡质堆积成帽状,也分为均匀的多层。由此说明泌蜡过程具有节律性。虫体周缘与蜡芒对应的突起区上分布着密集的刻点状腺孔,每一个腺孔分泌1根蜡丝,这在以往玻片标本中是观察不到的。雌性第3龄幼虫和成虫期,虫体分泌“湿蜡”,形成龟背状蜡壳,泌蜡腺孔主要为三格腺和四格腺。在肛突区发现了密集的泌蜡腺孔,排列为纵条纹状。从角蜡蚧蜡泌物甲酯化处理样品中检测到14个组分,从直接测试 (未经甲酯化处理) 样品中检测到14个组分;而从日本龟蜡蚧则分别检测到10个组分和25个组分。它们的主要成分是一系列高级的长链饱和与不饱和的烃、脂肪酸、脂肪醇、酯类、醛类以及杂环、多环或大环状化合物。对它们可能的生物生态学功能进行了讨论。     

云南双蜡蚧蜡泌物的超微形态与化学成分

采用扫描电镜、红外光谱、气相色谱/质谱联用技术研究了云南双蜡蚧Dicyphococcus bigibbus Borchsenius（同翅目：蚧科）蜡泌物的超微结构和化学成分。结果发现：该虫的若虫与成虫分泌的蜡质从蜡腺排出初期均为湿蜡,在体外凝结形成质地稍硬而脆的玻璃状干蜡壳,蜡壳具有均匀脊和横层。幼期和雄性蜡壳小,船形,周缘带有蜡角,中央隆起。雌成虫蜡壳大,元宝形。蜡泌物的红外光谱吸收峰密集,峰型尖锐,种的特征明显。用红外光谱特征推断蜡泌物的主要基团和化学构成在GC/MS检测中得到印证。用甲酯化和非甲酯化两种处理方法检测证明,该虫蜡泌物主要成分是一系列高级的长链饱和与不饱和的烃、脂肪酸及酯类化合物。化合物的碳原子数从C₁₄～C₄₀,大部分为偶数个,特别是碳链长度在C₃₀～C₄₀的酯类化合物种类多,比例大。还将该蚧虫蜡泌物的特征与蚧科内近缘的蜡蚧属种类及瘤坚大球蚧、朝鲜毛球蚧、背刺毡蜡蚧、白蜡虫等作了对比,阐述了蜡泌物在蚧虫分类、系统学及防治中的意义。     

中华绒螯蟹蜕皮抑制激素基因全长cDNA克隆和重组表达

根据实验室分离自中华绒螯蟹(Eriocheir sinensis)的一种蜕皮抑制激素(Molting-inhibiting hormone,MIH)N端氨基酸测序结果设计简并引物,采用RACE方法,首次从中华绒螯蟹眼柄中克隆到蜕皮抑制激素基因全长cDNA(Es-MIH,GenBank登录号:DQ341280),该基因全长为1457 bp,开放阅读框为330 bp,编码110个氨基酸(含有35个氨基酸的信号肽);其成熟肽包含C7-C44、C24-C40和C27-C53三个二硫键,有典型的CHH家族结构域。该cDNA编码的氨基酸序列与地蟹(Gecarcinus lateralis)MIH同源性最高,达到了85%。Northern杂交和半定量RT-PCR显示蜕皮间期成体蟹仅在眼柄中有MIH基因表达,提示该基因的表达具有一定组织特异性。利用pCR T7/NT TOPO TA系统重组表达MIH成熟肽,纯化的重组蛋白得率为0.3 g/L,纯化产物经质谱鉴定为中华绒螯蟹MIH。研究解决了CHH家族神经肽在机体中的表达量少,直接纯化较难的问题,为深入研究MIH的作用机制和在生产上控制中华绒螯蟹蜕皮和生长奠定了基础。     

短额负蝗与中华蚱蜢精子比较：直翅目：蝗总科

短额负蝗与中华蚱蜢精子比较（直翅目：蝗总科）奚耕思,郑哲民（陕西师范大学动物研究所陕西省西安市７１００６２）短额负蝗Ａｔｒａｃｔｏｍｏｒｐｈａｓｉｎｅｎｓｉｓ与中华蚱蜢Ａｃｒｉｄａｃｉｎｅｒｅａ为中国两种常见的蝗虫。由于头顶细纵沟,后足股节外侧花纹及...     

Multimodal cues drive host-plant assessment in Asian citrus psyllid (Diaphorina citri)

Asian citrus psyllid (Diaphorina citri) transmits the causal agent of Huanglongbing, a devastating disease of citrus trees. In this study we measured behavioral responses of D. citri to combinations of visual, olfactory, and gustatory stimuli in test arenas. Stimuli were presented to the psyllids in droplets or lines of an emulsified wax formulation in two different arena types in no-choice tests. First, when placed on a colored ring situated halfway between the center and perimeter of a petri dish, D. citri spent more time on yellow versus gray rings; however, this response disappeared when either gray or yellow wax droplets were applied. When the psyllids were presented with droplets scented with terpenes, the response to both scent and color was increased. The addition of a dilute (?0.1 M) sucrose solution to the wax droplets increased the magnitude of D. citri responses. Next, groups of D. citri were placed on plastic laboratory film covering a sucrose solution, to mimic a leaf surface. Test stimuli were presented via two 'midribs' made from lines of emulsified wax formulation. Probing levels were measured as a function of color saturation and scent composition, and concentration. The test scents were based on qualitatively major volatiles emitted by Murraya paniculata (L.) Jack, Citrus aurantifolia (Christm.) Swingle, and C. sinensis (L.) Osbeck. The highest probing response was observed on the middle concentration (20-μl scent/10 ml wax formulation) of the C. aurantifolia-scented wax lines. Results indicate that there are interactive effects between the different sensory modalities in directing host-plant assessment behavior.     

冬虫夏草菌无性阶段的分离和鉴定

分离工作在四川康定及北京进行。方法是将康定收集的14批新鲜冬虫夏草,经整体灭菌后,分别割取子座、柄和虫体三部,然后将各部再切割成绿豆大小样的接种体,置于含1％胨的PDA上,在20℃温箱中让其生长;也少量地进行了子囊孢子分离,结果获得90％以上是同一种分生孢子阶段真菌的培养物。而且对该培养物的鉴定表明,是被毛孢属(Hirsutella)一新种——中国被毛孢(Hirsutella sinensis sp.nov.)。并根据这些多批次且大数量接种体(1000个以上)、多途径且重复持续分离,连同子囊孢子分离以及1988年康定点上再次分离的相互验证,皆得到同一种真菌——中国被毛孢这一事实,我们初步结论:中国被毛孢是冬虫夏草菌[Cordyceps sinensis(Berk.)Sacc.]的无性阶段。     

中华鳖组蛋白H2A变体克隆及其在卵母细胞中的表达分析

为研究组蛋白H2A对龟鳖动物生殖细胞发育分化作用和机制, 克隆了中华鳖(Pelodiscus sinensis)组蛋白H2A变体的同源物(命名为PsH2A), 分析其转录本的表达模式及在卵巢发育成熟过程中的细胞定位。PsH2A cDNA序列全长575 bp, 5′端非编码区68 bp, 3′端非编码区108 bp, 开放阅读框399 bp, 编码133个氨基酸。氨基酸序列比对结果显示其与龟类的H2A变体同源性更高, 与哺乳类同源性较低。RT-qPCR和RT-PCR结果显示, PsH2A转录本在1冬龄、2冬龄和3冬龄的中华鳖卵巢中高表达(P<0.01), 而在精巢和其他成体组织中几乎检测不到。化学原位杂交结果显示, PsH2A mRNA在卵母细胞中特异性表达, 其中初级卵母细胞中表达信号最强, 且均匀的分布在细胞质中。随着卵母细胞发育成熟进入到生长期和成熟期后, 目的信号逐渐减弱, 并且主要在核周区域表达。此外, PsH2A mRNA的相对表达量也表现出中华鳖卵巢发育的季节性变化。综上, 研究结果表明PsH2A在中华鳖卵母细胞发育过程中可能发挥着重要作用。     

THE EFFECTS OF XANTHOTOXIN ON THE BIOLOGY AND BIOCHEMISTRY OF Galleria mellonella L. (LEPIDOPTERA: PYRALIDAE)

The effects of a dietary plant allelochemical, xanthotoxin (XA), on survivorship, development, male and female adult longevity, fecundity, and hatchability of the greater wax moth Galleria mellonella L. were investigated. Oxidative stress indicators, the lipid peroxidation product, malondialdehyde (MDA), and protein oxidation products, protein carbonyl (PCO) contents, and activities of a detoxification enzyme glutathione S‐transferase (GST) activity were determined in wax moth adults. The insect was reared from first‐instar larvae on an artificial diets containing XA at 0.001, 0.005, or 0.1% to adult stage in laboratory conditions. Relative to the controls, the diets containing XA concentrations led to decreased survivorship in seventh instar, pupal, and adult stages. Compared to control diet (77.7%), the highest dietary XA concentration decreased survivorship to adulthood to 11.0%. The highest XA concentration (0.1%) reduced female longevity from 10.4 to 5.7 days and decreased egg numbers from 95.0 to 33.5 and hatchability from 82.7 to 35.6%. The lowest XA concentration (0.001%) led to about a sixfold increase in MDA content. XA at high concentrations (0.005 and 0.1%) increased MDA (by threefold) and protein carbonyl (by twofold) contents decreased GST activity. The highest dietary XA concentration decreased GST activity from 0.28 ± 0.025 to 0.16 ± 0.005 μmol/mg protein/min. We infer from these findings that XA‐induced oxidative stress led to decreased biological fitness.     

白鱀豚额隆油、下颌油及皮脂油的脂肪酸组成

在齿鲸类的头部,有两个特殊的富含油脂的构造,即额隆和一对下颌脂肪体。在全身的皮下有很厚的皮脂层。Litchfield等(1974,1975)对齿鲸类中近20个属的额隆、下颌脂肪体和皮脂的脂质类型作比较研究,发现不同类群的油脂组分有差别,任何类群的额隆和下颌脂肪体的油脂--头脂的组分,与皮脂油的组分有明显区别,而额隆和下颌脂肪体的油脂组分是相近的。     

中华绒螯蟹对Pb和Cd的富集与释放特性

应用生物富集双箱动力学模型模拟了中华绒螯蟹(Eriocheir sinensis)分别在Pb浓度为0.25、0.50、0.75mg/L,Cd浓度为0.025、0.050、0.075mg/L的单一水环境中暴露时,蟹鳃、肝胰腺、肌肉和血淋巴对Pb和Cd的生物富集与释放实验,并通过非线性拟合得到中华绒螯蟹对Pb和Cd的富集速率常数k1、排出速率常数k2、生物富集系数BCF、生物半衰期B1/2、富集平衡时生物体内Pb和Cd含量CAmax等动力学参数。结果表明:(1)中华绒螯蟹对Pb和Cd具有明显的富集,蟹鳃、肝胰腺和肌肉中Pb和Cd的含量与富集时间和水环境中Pb和Cd暴露浓度表现出了很好的正相关,血淋巴在富集阶段没有明显的规律。理论平衡状态下鳃、肝胰腺和肌肉中Pb和Cd含量CAmax随着暴露浓度的增大而增大,且成正相关。(2)Pb和Cd在中华绒螯蟹组织器官中的富集具有选择性,开始实验前,Pb在中华绒螯蟹体内的的分布规律为:肝胰腺>鳃>肌肉>血淋巴;Cd的分布规律为:鳃>肝胰腺>血淋巴>肌肉。在实验浓度的Pb和Cd水环境中暴露16d后,Pb的分布规律为:鳃>肝胰腺>肌肉>血淋巴;Cd的分布规律为:肝胰腺>鳃>肌肉>血淋巴。(3)中华绒螯蟹对Pb和Cd的生物富集和释放都较缓慢。经过16d的生物富集,各组织器官中Pb和Cd的含量均未达到稳态平衡。Pb和Cd在组织器官中的生物富集系数(BCF)范围分别为5-51和6-3148,中华绒螯蟹对Cd的富集能力明显高于Pb(P1/2)范围分别为4-9d和8-57d,中华绒螯蟹对Cd的排出能力明显低于Pb。       

白蜡虫几个地方种虫经济性状的比较研究

本文对四川省几个地方种虫的虫囊含卵量、性比、虫囊大小分配情况、100粒虫囊重量、泌蜡期及泌蜡量等经济性状作了比较研究。     

竹类害虫的六种寄生蜂及二新种记述（膜翅目：跳小峰科）

本文报导与竹类害虫有关的６种跳小蜂。长缘刷盾跳小蜂Ｃｈｅｉｌｏｎｅｕｒｕｓｃｌａｖｉｇｅｒ及优赛跳小蜂Ｅｕｓｅｍｉｏｎｃｏｒｎｉｇｅｒｕｍ,寄生竹巢粉蚧Ｎｅｓｔｉｃｏｃｃｕｓｓｉｎｅｎｓｉｓ的初寄生者花翅跳小蜂Ｍｉｃｒｏｔｅｒｙｓｓｐｐ．;瓢虫隐尾跳小蜂Ｈｏｍａｌｏｔｙｌｕｓｆｌａｍｉｎｉｕｓ,寄生黑缘光瓢虫Ｅｘｏｃｈｏｍｕｓ（Ｘａｎｔｈｏｃｏｒｎｓ）ｎｉｇｒｏｍａｒｇｉｎａｔｕｓ;白胫短缘跳小蜂Ｚａｏｍｍａｅｒｉｏｃｏｃｉ（Ｔａｃｈｉｋａｗａ）中国新记录,寄生竹巢粉蚧的初寄生者花翅跳小蜂;四斑长索跳小蜂ＡｎａｇｙｒｕｓｑｕａｄｒｉｍａｃｕｌａｔｕｓＸｕｅｔＨｅ,ｓｐ．ｎｏｖ,寄生竹绒粉蚧Ｅｒｉｏｃｏｃｃｕｓｔｒａｎｓｖｅｒｓｕｓ;长脉卵跳小蜂ＯｏｅｎｃｙｒｔｕｓｌｏｎｇｉｖｅｎｏｓｕｓＸｕｅｔＨｅ,ｓｐ．ｎｏｖ,寄生竹卵圆蝽Ｈｉｐｐｏｔｉｓｃｕｓｄｏｒｓａｌｉｓ。标本及模式标本保存在浙江农业大学寄生蜂标本室。     

隐盾角蝉亚科Oxyrhachinae在中国的发现及一新种记述：同翅目：角蝉科

中国记录的角蝉原只属于露盾角蝉亚科Centrotinae。在对1991年从云南采集的角蝉标本的鉴定中,我们发现在我国有另一个亚科——隐盾角蝉亚科Oxyrhachinae的种类分布。本文报导了这一新记录亚科,并记述该亚科一新种。模式标本保存于西北农业大学昆虫博物馆。 隐盾角蝉亚科Oxyrhachinae,中国新记录亚科 该亚科于1929年由Haupt所建立,识别特征是小盾片完全被前胸背板所遮盖,前胸背板下缘和中胸前侧片下方各有一个向下后方伸出的齿突。大多种类分布于非洲,仅少数种类分布于亚洲和澳洲。 隐盾角蝉属Oxyrhachis Germar,中国新记录属 根据Capener(1962)的意见,隐盾角蝉亚科仅包括一个属——隐盾角蝉属Oxyrhachis Germar,该属的异名有Oxyrhachidia Melichar(1903),Xiphistes Stal(1866),Gongroneura Jacobi(1910),Xiphistoides Goding(1931),Kombazana Distant(1908)。中华隐盾角蝉Oxyrhachis sinensis,新种(图1-10) 体中型,雄虫较雌虫体小,体长5.7-6.5mm,上肩角顶端间宽2.6—3.5mm,肩角间宽2.3-2.6mm,红褐色。外形很近似于分布在印度、南非等地的Oxyrhachis tarandus(Fabricius),但区别在于:①成虫的上肩角比较上举,后突起不伸出前翅臀角;②若虫头顶突粗壮,前胸背板前突起顶端钝;③雄虫细胞减数分裂中期X染色体     

胡家溪中华新米虾种群动态、周年生产量及营养基础分析

2006年4月至2007年3月间对清江流域二级河流-胡家溪大型底栖动物群落的优势种中华新米虾Neocaridina denticulata sinensis(Kemp)进行为期1周年的调查研究。结果表明,中华新米虾的生活史为1年2代,中华新米虾的种群密度峰值分别出现在7月(31ind/m2)、10月(35ind/m2)、12月(20ind/m2)和次年的2月(20ind/m2),生物量峰值则出现在4月(1.76g/m2)、7月(4.74g/m2)和次年2月(2.38g/m2)。采用体长频率法(Size-frequency method)测算的周年生产量和P/B分别为67.40g/m2(湿重)和4.7。通过对中华新米虾前肠内含物组成的分析测定了各类食物类型的比例及对生产量的贡献率,结果表明,无形态碎屑和动物组织为其主要食物类型,分别占前肠内含物的79.9%和12.5%,对生产力的贡献率分别为62.50%和34.18%。       

中华倒刺鲃TRPV1基因的克隆及对高温和LPS刺激的响应

为探讨鱼类瞬时受体电位香草酸亚型1(TRPV1)的功能, 研究以本土淡水养殖鱼类中华倒刺鲃Spinibarbus sinensis为实验材料, 克隆其TRPV1基因, 开展生物信息学分析, 预测其蛋白结构, 并检测其组织表达谱。克隆所得到的TPRV1基因片段长2023 bp。该序列所编码的蛋白具有6次跨膜结构域、锚蛋白重复结构域和离子转运结构域。中华倒刺鲃TRPV1基因在脑的各部分区域及肠、肾等组织均有表达。为检验TRPV1基因的表达是否受到温度变化的调控, 实验鱼被分为磷酸缓冲液(PBS)和脂多糖(LPS)组, 并分别于不同温度(25℃和35℃)处理1h、6h和24h后, 检测TRPV1在间脑和延脑的表达量变化。结果显示温度变化和LPS处理会引起该基因在间脑和延脑表达量的明显变化, 但两种组织的变化模式相反, 且具有时间特异性。综上, 中华倒刺鲃TRPV1的表达具有温度和体内炎症的敏感性, 并可能具有组织和时间的特异性。     

Wax glands ultrastructure and chemical composition of wax of giant mealybug Drosicha stebbingii (Green) (Hemiptera: Monophlebidae)

Droschia stebbingii (Green) belonging to the family Monophlebidae (Hemiptera: Coccoidea) is a serious, phytophagous and dimorphic pest. D. stebbingii and D. mangiferae (Green) with sixty-two common hosts and minor morphological variations pose problems towards their taxonomic identification. Light and scanning electron microscopy were used to outline the ultrastructural details of cuticular wax producing structures of D. stebbingii. Three types of wax-pores along with tubular duct were observed on the head, thorax and abdomen. Quinquelocular and multilocular disc-pores exhibited variations in the central loculus with single and double opening. Short curly wax strands were secreted from the outer loculi whereas central loculus secreted long tubular strands. Additionally, bilocular pores secreting curly wax were observed having two large loculi separated by a septum. GC–MS analysis of cuticular wax indicated the presence of hydrocarbons and wax esters forming majority of the covering along with long chain alcohols, ketones and acids. Variations in the percentage of hydrocarbon and wax esters content were observed for two populations of D. stebbingii collected from different hosts. Hydrocarbons were abundant in populations collected on Pongamia pinnata (L.) Pierre whereas long chain esters dominated the wax profile for the population collected from Tectona grandis L.f. Compositional variations in the cuticular wax of D. stebbingii on different host along with knowledge on the ultrastructural wax pores and waxy secretion would provide an insight to the existing knowledge of this scale insect.