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土人参的组织和单细胞培养及试管苗开花结实 总被引:12,自引:0,他引:12
以土人参的花梗、茎和叶片为外植体在MS培养基上诱导出愈伤组织,诱导率为75%-90%。愈伤组织经分化和生根培养再生了完整植株。由组织培养再生苗的幼茎诱导的愈伤组织建立悬浮系。由悬浮系分离的单细胞在2/3MS液体培养基中振荡培养或振荡培养3周后转入双层培养均再生了愈伤组织,再生率分别为0.28%和0.41%。愈伤组织在含有较低浓度6-BA的培养基上分化出苗。幼苗生长迅速,每3周扩增6.7倍,再生植株 相似文献
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草木樨状黄芪高频离体再生体系的建立 总被引:12,自引:0,他引:12
以草木樨状黄芪无菌苗茎切段为材料,在含1-2mg/L2,4-D和0-0.5mg/L6BA的MS培养基上培养获得大量愈伤组织,愈伤组织诱导率在95%以上,愈伤组织在附加0.2mg/LKT,1mg/L6BA,0或0.5mg/LNAA,500mg/LCH 和200mg/L YE的MS培养基上诱导丛生芽,并进而发育成苗。苗的分化频率为100%。分化苗或其茎的切段在不国源植物激素的1/2MS培养基上可出现根的分化,分化频率达90%以上,再生植株经炼苗后移栽成活率达80%以上。 相似文献
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沙冬青愈伤组织对培养基的特殊要求和球形胚状分化结构的诱导 总被引:5,自引:2,他引:3
通过对沙冬青未成熟子叶进行离体培养,获得了愈伤组织。基本培养基采用B5,再配合MS培养基的铁盐成分,对沙冬青未成熟子叶愈伤组织的诱导和生长效果最佳。愈伤组织的诱导和生长对培养基的附加成分要求十分严格,2,4-D浓度为0.5mg/L,6-BA浓度为0.5mg/L,蔗粮不宜超过2%。愈伤组织最初为乳白色透明松散状态,生长比较缓慢,极易褐化死亡。如果培养基中的蔗糖浓度低于2%,两周后未成熟子叶愈伤组织逐 相似文献
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栝楼的快速繁殖及愈伤组织的诱导 总被引:15,自引:0,他引:15
本文对栝楼的快速繁殖及愈伤组织的诱导进行了初步研究。结果表明:含腋芽的栝楼外植体在MS+6-BA1.0+NAA0.2上可以快速繁殖;茎切段、叶片切块及子叶切块在MS+2,4-D1.0+6-BA0.5上能够形成愈伤组织但以茎段形成愈伤组织的速度最快,质量最好;愈伤组织在MS+6-BA1.0+NAAO.1上分化成芽。 相似文献
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苦丁茶愈伤组织的诱导与褐变抑制 总被引:5,自引:0,他引:5
以叶片为材料,对苦丁茶愈伤组织的诱导,继代培养及褐变调控的研究表明:(1)苦丁茶愈伤组织的诱导及继代培养均以MS附加BA 2.0mgL^-1和NAA 4.0mgL^-1的培养基效果最好;(2)0.1%的植酸可明显促进愈伤组织生长、抑制褐变,而硫代硫酸钠效果最差;(3)连续培养40-50d愈伤组织增长倍数达到最大值;(4)继代27次以后愈伤组织生长速度开始下降。这些条件为下一步细胞培养生产苦丁茶甙等 相似文献
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一品红离体培养诱导体细胞胚的研究(简报) 总被引:5,自引:0,他引:5
一品红的嫩茎离体培养在附加不同浓度的2,4-D和6-BA的MS培养基上,2.4D浓度为1.0~2.0 mg· L~(-1)的愈伤组织生长量最大。这些愈伤组织转移到MS+6-BA1.0 mg·L~(-1)+NAA0.1 mg·L~(-1)的分化培养基上后,产生了胚性愈伤组织,观察到大量不同时期体细胞胚。带有体细胞胚的愈伤组织进一步转移到无激素 MS培养基上,便发育成小植株。 相似文献
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红豆草耐盐愈伤组织的筛选及植株再生 总被引:13,自引:3,他引:10
将红豆草种子在含1.2%NaCl的MS培养基上萌发以消除盐敏感的幼苗,把存活的幼苗下胚轴切段在含1mg/L2,4-D、0.5mg/L6-BA及1.2%NaCl的MS培养基上诱导愈伤组织,通过连续筛选得到可耐受1.8%NaCl的愈伤组织,在有0.2mg/L NAA和1mg/L IAA存在下该愈伤组织分化出芽,待幼,待幼苗长至3cm左右时转至含2mg/LNAA和或IBA的1/2MS培养基上生根。对对照 相似文献
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茶(Camellia sinensis Kuntze)离体培养体细胞胚胎发生的组织细胞学研究 总被引:3,自引:0,他引:3
以茶树叶片为外植体.以MS培养基附加4mg·L-16-BA,2mg·L-1IAA.3mg·L-1GA3和0.2—0.3%活性碳诱导出愈伤组织和胚状体,进一步形成小植株.切片观察表明.茶叶愈伤组织胚状体的发生,起源于愈伤组织表层及其内部的单个细胞和细胞团,胚状体发育顺序与合子胚大致相似.经球形胚、心形胚、鱼雷胚和子叶胚阶段.但发育过程中常有畸形胚出现. 相似文献
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紫杉醇及其衍生物的研究概况与发展趋势 总被引:4,自引:0,他引:4
本文分别从生物资源,药理学,化学合成和组织培养方面对红豆杉植物中紫杉醇及其衍生物的研究工作进行了综述,从中可以了解紫杉醇及其衍生物的研究概况与发展趋势。 相似文献
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David W. Bierhorst 《American journal of botany》1971,58(7):634-648
The gametophyte, old embryo, and sporophyte of Schizaea pseudodichotoma sp. nov., sporophyte and female parental gametophyte of S. diversispora hybr. nov. (S. pseudodicholoma X probably S. dichotoma), sporophyte of S. rhacoindusiata sp. nov., and gametophyte, old embryo, and sporophyte of Actinostachys macrofunda sp. nov. are described. The taxonomy of Schizaea is discussed and the system of Diels is strongly supported. The two sectional names used by Diels, Euschizaea Hook, and Lophidium Rich, are replaced by Pectinatae Prantl and Schizaea respectively. The prime morphological significance of Schizaea pseudodicholoma lies in its leafless embryo and its simple leaf differing from other species in its section, and that of Actinostachys macrofunda lies in its reduction to nearly complete heterotrophic existence and its frequent multiple annulus. Fungal hyphae have been traced from Schizaea and Actinostachys through the substratum and into root nodules of Casuarina and into roots of two other angiosperms. 相似文献
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DISTRIBUTION OF AMINO ACIDS AND OF EXO- AND ENDOPEPTIDASES ALONG VERTEBRATE AND INVERTEBRATE NERVES 总被引:1,自引:1,他引:0
Abstract— The proximo-distal gradients for representative peptidases, peptidylpeptide hydrolases, and amino acids were measured in segments of peripheral nerve from invertebrates and vertebrates and in the lobster brain and ventral cord.
Crustacean nerve was characterized by a large pool of free amino acids totaling 100–200 μmoles/g wet wt. In lobster nerve, the principal free amino acid was aspartic acid which comprised 55 per cent of the free pool, whereas in the rat sciatic nerve it comprised only 5 per cent. The principal free amino acid in rat sciatic nerve was taurine (32 per cent of the pool) and in lobster brain glycine comprised 30 per cent of the pool. No consistent patterns emerged for the gradients along the nerves for amino acids and hydrolytic enzymes. In the leg nerve of the lobster, concentrations of aspartic acid and arginine were higher in the proximal region, and concentrations of proline and alanine were higher in the distal region. Concentrations of most amino acids were higher in the proximal regions of crab nerve, of lobster brain and ventral cord, and of rat sciatic nerve.
Rat sciatic nerve exhibited a pronounced proximo-distal increase in activity of aminopeptidase (Leu-Gly-Gly). In lobster leg nerve, activity of neutral proteinase was higher in the proximal segment, whereas activity of acid proteinase was higher in the distal segment. The best examples of proximo-distal gradients were found in lobster brain and ventral cord; activities of endopeptidases, arylamidases (Leu- and Arg-βNA), and aminopeptidase were higher in the supra-esophageal ganglia or cephalothorax segments than in the distal regions. 相似文献
Crustacean nerve was characterized by a large pool of free amino acids totaling 100–200 μmoles/g wet wt. In lobster nerve, the principal free amino acid was aspartic acid which comprised 55 per cent of the free pool, whereas in the rat sciatic nerve it comprised only 5 per cent. The principal free amino acid in rat sciatic nerve was taurine (32 per cent of the pool) and in lobster brain glycine comprised 30 per cent of the pool. No consistent patterns emerged for the gradients along the nerves for amino acids and hydrolytic enzymes. In the leg nerve of the lobster, concentrations of aspartic acid and arginine were higher in the proximal region, and concentrations of proline and alanine were higher in the distal region. Concentrations of most amino acids were higher in the proximal regions of crab nerve, of lobster brain and ventral cord, and of rat sciatic nerve.
Rat sciatic nerve exhibited a pronounced proximo-distal increase in activity of aminopeptidase (Leu-Gly-Gly). In lobster leg nerve, activity of neutral proteinase was higher in the proximal segment, whereas activity of acid proteinase was higher in the distal segment. The best examples of proximo-distal gradients were found in lobster brain and ventral cord; activities of endopeptidases, arylamidases (Leu- and Arg-βNA), and aminopeptidase were higher in the supra-esophageal ganglia or cephalothorax segments than in the distal regions. 相似文献
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Norman H. Boke 《American journal of botany》1963,50(8):843-858
Boke, Norman H. (U. Oklahoma, Norman.) Anatomy and development of the flower and fruit of Pereskia pititache. Amer. Jour. Bot. 50 (8): 843–858. Illus. 1963.—Flowers of P. pititache are about 6 cm in diameter and perigynous. The receptacle bears numerous broad bracts; the inner perianth segments are orange and deeply cleft; the numerous stamens develop centrifugally. The fundamentally superior gynoecium is broad and flat and consists of 10–18 connate carpels, the fertile portions of which are involute and adnate to the conical floral axis. The 8–16 ovules in each of the pocket-like locules are borne in 2 rows along the zone of adnation; placentation is axile. The short style and the style branches are lined with stigmatic tissue, which extends downward among the ovules. There is no definite stigma. The tip of the floral axis retains its meristematic characteristics beyond anthesis. In early fruit development, the rim of the floral cup grows in height, while the residual floral axis becomes a conspicuous peg-like columella. Concomitantly, the formation of numerous mucilage cells and cavities causes the ovary partitions and parts of the ovary roof to disintegrate. As a result, the seeds are contained in a single, moat-like cavity, which appears inferior. Late fruit development is characterized by the differentiation of numerous fiber sclereids in association with the extensive and complex vascular system. 相似文献