| 枸杞髓组织离体培养及高频率植株再生的研究 |
| |
| 引用本文： | 曹有龙, 陈放, 罗青, 曲琳,.枸杞髓组织离体培养及高频率植株再生的研究[J].广西植物,1999,19(3):239-242+292. |
| |
| 作者姓名： | 曹有龙 陈放 罗青 曲琳 |
| |
| 作者单位： | 1. 四川大学生物系,四川,成都,610064
2. 宁夏农科院农业生物技术研究室,宁夏,银川,750002 |
| |
| 摘 要： | 枸杞髓组织在４种ＭＳ培养基上都能诱导出愈伤组织，诱导率５３．７％～１００％。在培养基ＭＳ＋６－ＢＡ０．１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ获得的愈伤组织，呈颗粒状，分散性能好，胚性细胞多．将其转移到ＭＳ＋６－ＢＡ０．５ｍｍ／Ｌ＋ＮＡＡ０．０１ｍｇ／Ｌ的分化培养基上获得大量绿色小芽，小芽在ＭＳ＋６－ＢＡ０．２ｍｇ／Ｌ的培养基上得到快速繁殖，繁殖系数５０～１５０株／芽·月。丛生芽在ＭＳ＋ＮＡＡ０．２ｔｍｇ／Ｌ的培养基上形成完整植株
|
| 关 键 词： | 枸杞 髓组织 离体培养 植株再生 |
| 修稿时间： | 1998-08-09 |
In vitro culture of pith tissue and high-frequency plant regeneration of Lycium barbarum L. |
| |
| CAO You-long,CHEN Fang,LUO Qing,QU Lin.In vitro culture of pith tissue and high-frequency plant regeneration of Lycium barbarum L.[J].Guihaia,1999,19(3):239-242+292. |
| |
| Authors: | CAO You-long CHEN Fang LUO Qing QU Lin |
| |
| Abstract: | A study on Pith tissue culture on MS medium with different hormone, the induction ratew 53.7% ~100%. Among which the calli induced on medium MS+6-BA 0.1mg / L+NAA 0.5mg / L were smallest in Particle size and had good dispersion ability with a large quantity of embryonic Cells The cant were moved onto Ms+6-BA 0.5 mg / L+NAA 0.01mg / L differentiation medium and a large quantity of embryonic Plants were obtained The embryonic plants were reproduced rapidly after they are removed onto medium MS+6-BA 0.2mg / L with the reproductionrate of 50~150 clusters shoot / bud.month. Complete plants were induced on MS+NAA 0.2mg / Lmedium |
| |
| Keywords: | Lycium barbarum L Pith tissue culture in vitro culture plant regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《广西植物》浏览原始摘要信息 |
| 点击此处可从《广西植物》下载免费的PDF全文 |
