草木樨状黄芪高频离体再生体系的建立

以草木樨状黄芪无菌苗茎切段为材料，在含１－２mg/L2,4-D和０－０．５mg/L６ＢＡ的MS培养基上培养获得大量愈伤组织，愈伤组织诱导率在９５％以上，愈伤组织在附加０．２mg/LＫＴ，１mg/L６ＢＡ，０或０．５mg/LＮＡＡ，５００mg/LＣＨ 和２００mg/L ＹＥ的ＭＳ培养基上诱导丛生芽，并进而发育成苗。苗的分化频率为１００％。分化苗或其茎的切段在不国源植物激素的１／２ＭＳ培养基上可出现根的分化，分化频率达９０％以上，再生植株经炼苗后移栽成活率达８０％以上。

Establishment of a high efficient regeneration system from internode segments of seedling in Astragalus meliloides Pall.

Calluses were induced form internode segments of sterile seedlings of Astragalus melilotoides Pall on MS medium supplemented with 1-2 mg/L 2,4 D and 0-0.5 mg/L 6BA.The frequency of callus induction was above 95%.When calluses were transferred and cultured on MS medium supplemented with 0.2 mg/L KT,1 mg/L 6BA,0 or 0.5 mg/L NAA,500 mg/L CH and 200 mg/L YE,numerous shoots were formed.The differentiation frequency was 100%.The regenerated shoots or shoot cuttings produced roots on 1/2 MS without any phytohormones.The regenerated plantlets were transplanted into the soil.Over 80% transplanted plants were survival.