共查询到20条相似文献,搜索用时 46 毫秒
1.
大叶秦艽的组织培养与植株再生 总被引:7,自引:0,他引:7
以秦艽的叶和下胚轴为外植体,成功地诱导出愈伤组织和再生植株.诱导愈伤组织最合适的培养基为附加2 m g·L- 1 2 ,4 - D和0 .5 mg·L- 1 6 - BA的MS培养基,诱导率可达到10 0 % .愈伤组织转移到附加2 mg·L- 12 ,4 - D和0 .5 mg·L- 1 KT和5 0 0 m g·L- 1 L H的MS培养基上进行继代培养,增殖后的愈伤组织转移到附加0 .1mg·L- 1 2 ,4 - D和0 .5 m g·L- 1 6 - BA的MS分化培养基上进行分化,其分化率可达到86 .6 7% ,将分化出的芽转接到不加激素的MS上,结果可生长出大量的分化苗. 相似文献
2.
海滨锦葵胚轴愈伤组织诱导及植株再生 总被引:1,自引:0,他引:1
以海滨锦葵(Kosteletzkya virginica)胚轴为外植体,在9种不同激素配比的培养基上进行愈伤组织诱导、继代培养、不定芽分化及生根培养,确定了植株再生的最适培养条件:(1)愈伤组织诱导最适培养基为MS+IAA 1.0 mg·L-1+KT 0.3 mg·L-1+sucrose 30 g·L-1+agar 8 g·L-1,愈伤组织诱导率为93.94%;(2)不定芽诱导最适培养基为MS+IAA 0.1 mg·L-1+ZT 0.5 mg·L-1+sucrose 30 g·L-1+agar 8 g·L-1,不定芽诱导率为65.83%;(3)生根最适培养基为MS+sucrose 30 g·L-1+ agar 8 g·L-1,生根率为96.67%。炼苗移栽后,成活率可达85%。 相似文献
3.
虎杖的组织培养与快速繁殖 总被引:15,自引:0,他引:15
以虎杖茎段、叶柄、叶片为外植体探讨了愈伤组织诱导、分化和植株再生的条件,筛选出茎段生长培养基为1/2MS+BA1.0mg·L-1+KT0.5mg·L-1+NAA0.2mg·L-1,茎段、叶柄和叶片外植体愈伤组织诱导培养基为MS+BA1.0~2.0mg·L-1+KT0.2~0.5mg·L-1+NAA0.2~0.5mg·L-1或MS+BA2.0~3.0mg·L-1+KT0.2~0.5mg·L-1+2,4-D0.5mg·L-1;丛生芽诱导培养基为MS+BA2.0mg·L-1+KT0.5mg·L-1+IBA0.2mg·L-1+LH1000;不定根及根状茎诱导培养基为1/2MS+IBA0.2mg·L-1. 相似文献
4.
5.
土人参茎尖培养和植株再生 总被引:4,自引:0,他引:4
1 植物名称 土人参 (Talinumpaniculatum)。2 材料类别 实生苗之茎尖 ,种子取自本校花圃。3 培养条件 种子萌发培养基 :( 1 ) 1 /2MS +0 .7%琼脂 + 2 %蔗糖。丛生芽诱导培养基 :( 2 )MS + 6 BA 0 .5mg·L- 1 (单位下同 ) ;( 3)MS +NAA0 .5 + 6 BA 2 .0 ;( 4 )MS +NAA 0 .5 +KT 2 .0 ;( 5 )MS+NAA 0 .5 + 6 BA 3.0。丛生芽增殖培养基 :( 6)MS + 6 BA 2 .0 ;( 7)MS +NAA 0 .0 5 + 6 BA 3.0。生根培养基 :( 8) 1 /2MS +NAA 2 .0。上述 ( 2 )~ ( 8)培养基均附加 30 g·L- 1 蔗糖、7g·L- 1 琼脂 ,pH 5 .8。培养室温… 相似文献
6.
雾灵山地区野生樱花的组织培养与快速繁殖 总被引:8,自引:0,他引:8
1 植物名称 樱花 (Prunusserrulata) ,又名山樱花。2 材料类别 5月初采集河北省兴隆县雾灵山北麓的野生樱花 ,取叶腋处未见任何突起的带叶柄茎段为外植体。3 培养条件 诱导腋芽培养基 :( 1 )MS + 6 BA 2 .0mg·L- 1 (单位下同 ) +IAA 0 .5 ;( 2 )MS + 6 BA 2 .0+IAA 1 .0 ;( 3)MS + 6 BA 2 .0 +IAA 1 .5 ;( 4 )MS+ 6 BA 3.0 +IAA 0 .5 ;( 5 )MS + 6 BA 3.0 +IAA1 .0 ;( 6)MS + 6 BA 3.0 +IAA 1 .5。诱导丛生芽与芽增殖培养基 :( 7)改良MS(NH4NO3、KNO3减半 ,MgSO4·7H2 O、ZnSO4·7H2 O加倍的MS) + 6 BA … 相似文献
7.
番茄下胚轴离体培养植株再生及其组织学观察 总被引:7,自引:2,他引:5
研究了上海地区的主要栽培品种之一“鲜丰”番茄下胚轴离体培养过程中的激素调控 ,结果表明 :“鲜丰”番茄下胚轴进行离体培养过程中 ,MS培养基上附加不同浓度的生长素( IAA)和细胞分裂素 ( BA) ,对愈伤组织的形成影响不大 ,但对不定芽的分化有较大的影响 ,得出最佳培养基为 MS+ BA1 .0~ 2 .0 mg· L- 1+ IAA0 .2 mg· L- 1。用不同浓度的 ZT、BA、KT进行单因子芽器官的诱导实验 ,发现 ZT的作用力强于 BA和 KT,KT最弱 ;用不同浓度NAA、IAA、IBA、2 ,4- D进行发根培养实验 ,发现番茄的内源生长素浓度较高 ,用外植体直接发根外加生长素有一定的作用 ,若用不定芽扦插发根 ,不附加生长素也极易发根 ,故番茄的生根培养基为 1 /2 MS或 MS附加 IAA 0 .0 5~ 0 .1 mg· L- 1。另外 ,对有关细胞启动、分裂、分化以及器官发生的组织学观察表明 :番茄离体培养中不定芽通常发生在愈伤组织的周边区 ,也可起源于维管组织结节周围的形成层状细胞。不定根则由茎中柱鞘处发生。 相似文献
8.
蒟蒻薯的组织培养 总被引:3,自引:0,他引:3
1 植物名称 薯 (Taccachantrieri) ,又名老虎须、箭根薯。2 材料类别 叶柄。3 培养条件 以MS为基本培养基。 ( 1 )愈伤组织诱导培养基 :MS + 6 BA 1mg·L- 1 (单位下同 ) +NAA 0 .2 ;( 2 )芽分化和增殖培养基 :MS + 6 BA 1~ 2 +NAA 0 .2 ;( 3)生根培养基 :1 /2MS + 6 BA 0 .5 +NAA 1。以上培养基中均加 5 g·L- 1 琼脂粉 ,培养基 ( 1 )和 ( 2 )加 30 g·L- 1 白糖 ,( 3)加 2 0 g·L- 1 白糖、0 .5 g·L- 1 活性碳 ,pH 5 .8。培养温度为 2 5~2 8℃ ,光照 1 0~ 1 2h·d- 1 ,光照度 1 5 0 0~ 2 0 0 0lx。4 生长与分… 相似文献
9.
1 植物名称 海州蒿 (Artemisiafauriei)。2 材料类别 茎尖。3 培养条件 愈伤组织诱导培养基 :(1)MS +6 BA1mg·L- 1(单位下同 ) +NAA 1;(2 )MS +6 BA 1+NAA 0 .5 ;(3)MS +6 BA 2 +NAA 0 .5。芽分化培养基 :(4 )MS +6 BA 1;(5 )MS +6 BA 2 ;(6 )MS +6 BA 5 +NAA 0 .1;(7)MS +6 BA 2 +NAA 0 .0 1。生根培养基 :(8) 1/2MS ;(9) 1/2MS +NAA 0 .1;(10 )MS。以上培养基中的蔗糖含量除生根培养基 (8)~(10 )为 2 0 g·L- 1外 ,其余均为 30 g·L- 1。pH 5 .8~6 .0。培养温度 (2 5± 1)℃ ,光照时间 14h·d- 1,… 相似文献
10.
地灵的组织培养及快速繁殖 总被引:3,自引:0,他引:3
1 植物名称 地灵 (Stachyssieboldii)。2 材料类别 根状茎。3 培养条件 起始培养基 :1 /2MS ,不添加任何激素。诱导愈伤组织培养基 :( 1 )MS + 6 BA 1 .2mg·L- 1 (单位下同 ) +NAA 0 .6;( 2 )MS + 6 BA 1 .8+NAA 0 .6;( 3)MS + 6 BA 2 .4+NAA 0 .6;( 4 )MS+ 6 BA 3+NAA 0 .2 ;( 5 )MS + 6 BA 2 +NAA 0 .1 ;( 6)MS + 6 BA 2 .5 +NAA 0 .1。诱芽培养基 :( 7)MS + 6 BA 2 +KT 0 .1 +NAA 0 .1。增殖培养基 :( 8)MS + 6 BA 2 +NAA 0 .2。诱导愈伤组织和芽的培养基均添加 4%蔗糖 ,增殖培养基添加 3%蔗糖 ,琼脂 0 … 相似文献
11.
12.
Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
13.
14.
15.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
16.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
17.
18.
19.
RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
20.
JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献