中国新纪录属——平织蛾属Erotis(鳞翅目:织蛾科)研究(英文)

首次报道了平织蛾属Erotis Meyrick在中国的分布,并描述了2新种:阔平织蛾Erotis expansasp.nov.和点平织蛾Erotis punctalosa sp.nov.。阔平织蛾采自贵州江口,与Erotis phosphoraMeyrick相似,但其雄性外生殖器的爪形突较宽大,后缘中部微凹;颚形突几乎与爪形突等长;抱器背平直。点平织蛾采自广西防城,与阔平织蛾Erotis expansa sp.nov.外形近似,但其雌性第八腹节背板后缘较平直;囊突角状,基部骨化板为不规则三角形,其前缘有微刺。文中给出了该属世界已知种检索表。模式标本保存在南开大学生物系。     

中国隐织蛾属研究(鳞翅目:织蛾科)(Ⅱ):贵州种类记述(英文)

依据从2000年到2002年采自贵州赤水、习水以及梵净山等地的隐织蛾属Cryptolechia Zeller昆虫标本,研究报道了中国贵州的隐织蛾昆虫14种,包括11新种:拟弯带隐织蛾C.proximideflecta sp.nov.,苔隐织蛾C.muscosa sp.nov.,独带隐织蛾C.solifasciaria sp.nov.,棒隐织蛾C.spinifera sp.nov.,齿隐织蛾C.denticulata sp.nov.,叉隐织蛾C.furcellata sp.nov.,阔隐织蛾C.latifascia sp.nov.,尖隐织蛾C.acutiuscula sp.nov.,束隐织蛾C.fasciculifera sp.nov.,钩隐织蛾C.hamatilis sp.nov.和凹隐织蛾C.concaviuscula sp.nov.。首次报道了葛氏隐织蛾C.gei Wang的雌性个体。文中提供了新种的外生殖器特征图。模式标本保存在南开大学生物系。     

中国铠甲属Micropeplus一新种(鞘翅目:隐翅虫科:铠甲亚科)(英文)

记述铠甲属一新种,微齿铠甲Micropeplus dentatus,sp.nov.,新种采自浙江省镇姚。新种鞘翅具两条盘脊,体表无刻点,但具革质刻纹,属于tesserula group。这是该类群在我国的第一次纪录。新种与日本种Micropeplus sharpi Sawada极为相似,新种中足胫节内缘具11个小齿与日本种的8个小齿明显不同。新种主要鉴别特征为:头顶中脊发达,鞘翅具伪缘折脊,中足胫节内缘具11个小齿。模式标本保藏在中国科学院动物研究所标本馆内。     

转RGSV—SP基因水稻植株的再生

通过农杆菌介导将水稻草矮病毒(Rice grassy stunt virus,RGSV)编码病害特异蛋白基因sp导入台农67及中花6号品种。经过筛选,再生,获得转化植株。PCR及Southern点杂交分析结果初步表明目的基因片段整合到水稻基因组中。RT—PCR Southern点杂交结果表明目的片段已在植株体内进行了转录。     

淡黄花百合的组织培养与快速繁殖

以淡黄花百合的鳞片为外植体进行试管培养,筛选出各培养阶段适宜的培养基分别为:(1)丛生芽诱导,MS 6-BA 2.0 mg/L NAA 0.2 mg/L 蔗糖3%;(2)继代增殖,MS 6-BA 1.5 mg/L NAA 0.1 mg/L 蔗糖3%;(3)生根及小鳞茎生长,1/2 MS NAA 0.5～1.0 mg/L 蔗糖3% 活性碳0.1%,1/2 MS NAA 0.5 mg/L 蔗糖6% 活性碳0.1%.     

超临界柚子鲜花芳香性成分的研究

本文介绍了采用超临界CO2萃取新鲜柚子花中芳香性成分,并用气相色谱-质谱联用仪对其中化学成分进行分析鉴定.共鉴定出31个芳香性有机化学成分,占超临界CO2萃取柚子鲜花总芳香油的78.886%.通过对柚子花芳香性成分的分析,为开发高附加值的柚子花香精香料提供科学依据.     

TPA对Bcl-X前体mRNA在U251细胞中选择性剪接调控的初步研究

以含有部分Bcl-X序列的微小基因为研究模型,研究了TPA对Bcl-X前体mRNA选择性剪接在体内和体外的调控作用。通过RT-PCR和定点突变实验检测,结果表明PKC信号系统能调控Bcl-X前体mRNA选择性剪接,其机制可能是通过应答序列来调控Bcl-X前体mRNA5’上游和下游剪接位点的选择利用。     

一种毛翅目昆虫的生态治理研究

针对生活在水流速度较快的湖南省柘溪水电站一带河床大量发生的原双栖纹石蛾(Amphipsyche proluta)来说采用一般的防治措施都很难付诸实施,或者只能是治标,不能治本。经1997年至2000年的调查研究和试验分析得知人为去掉河底大型石块,加深河水深度,减缓流速,以破坏适宜于该石蛾幼虫生存的生态环境,引进生态致死因子,是从根本上杜绝该石蛾发生的较为理想的办法。     

大孢虫花生物学特性及驯化栽培

对采自辽宁省仙人洞国家级自然保护区野生大孢虫花(Isaria japonica Yasuda)的生物学特性和驯化栽培进行探究。试验结果表明:PDA培养基上适宜碳、氮源分别是蔗糖和谷氨酸,适宜p H为6;在3种供试培养料中只能在配方一(2%葡萄糖,0.1%硫酸镁,0.2%磷酸二氢钾,1.4%琼脂,96%蒸馏水,p H自然,0.3%蛋白胨)中形成子实体。     

Diapause development in early and late emerging phenotypes of Delia floralis

The turnip fly, Delia floralis Fall6n （Diptera： Anthomyiidae） is an important insect pest of brassica vegetable crops in the holarctic region. Different populations have strongly varying temperature requirements for fly emergence, a challenge for accurate prediction of activity. This study focused on diapause development in one early and one late emerging phenotype. The physiological state after various treatments was deduced from emergence data. Our results showed a slow diapause progression at chilling conditions for both populations and diapause ended about 7 months after pupae were formed for the early population. For the late population held at 4℃ diapause did not end, no matter how long the duration of chilling. These pupae required a period with elevated temperatures above 6~C to continue development. At constant non-chilling conditions （18℃） from the time pupae were formed both populations completed diapause most rapidly. These results indicate that chilling delayed, rather than accelerated development and was not a prerequisite for diapause development. For post-diapause, results indicated a linear relationship between rate of development and temperature within the range of 6-18℃and a theoretical base temperature for development of about 2℃ for both populations. In conclusion, D. floralis pupae are in diapause throughout a long winter period, and delayed emergence of the late population appears to be caused by prolonged diapause regulated by a developmental temperature threshold. The study has added information on the biology of turnip fly populations, a prerequisite for improved pest control.