披针叶屈曲花的组织培养和快速繁殖

1植物名称披针叶屈曲花(Iberis intermdia Guersent.)。2材料类别带芽茎段、叶片。3培养条件诱导培养基:(1)MS 6-BA 1.0mg·L~(-1) (单位下同) NAA 0.1 3%蔗糖,(2)MS 6-BA 1.0 NAA 0.1 2,4-D 0.1 3%蔗糖。增殖分化培养基:(3)MS 6-BA 2.0 NAA 0.1 3%蔗糖,(4)MS 6-BA 2.0 NAA 0.1 2,4-D 0.1 3%蔗糖。生根壮苗培养基:(5)1/2MS 6-BA 0.5 NAA 0.1 GA_3 0.5 3%蔗     

悬垂秋海棠的组织培养

(1)MS 6-BA 0.5 mg·L-1(单位下同);(2)MS 6-BA 1.0;(3)MS 6-BA 0.5 NAA 0.2(4)MS 6-BA 1.0 NAA0.2:(5)MS 6-BA 0.5 NAA 0.1;(6)1/2MS NAA0.1.以上均加入3%蔗糖、0.65%卡拉胶,pH5.8.培养温度(25±1)℃,光照度3000lx.光照时间10h·d-1.     

达摩兰组织培养适宜培养基的研究

以达摩兰的原种苗茎段为外植体进行组织培养研究,筛选出最适培养基(1)原球茎诱导:MS+6-BA 0.5 mg/L+NAA 1.0 mg/L+香蕉肉250 g/L;(2)丛生芽诱导:1/2 MS+6-BA 3 mg/L+NAA 2.0 mg/L+AC 2.5 g/L+蔗糖3%+香蕉肉200 g/L;(3)芽增殖:1/2 MS+6-BA 3 mg/L+NAA 2.0 mg/L+AC 2.5 g/L+蔗糖3%+香蕉肉150 g/L;(4)根诱导:1/2 MS+6-BA 0.3 mg/L+IBA 0.5 mg/L+NAA 0.5 mg/L+AC 2.5 g/L+蔗糖3%。采用珍珠岩、细石与树皮的混合物为培养基质,移栽成活率达95%以上。     

枸杞的组织培养与快速繁殖(简报)

本试验以枸杞嫩枝及顶芽作外植体进行组织培养。结果表明,不定芽诱导用 MS 6-BA 1.0mg/L NAA 0.1mg/L;增殖培养基以 MS 6-BA 0.5mg/L IBA 1.0mg/L 较佳;生根培养基为 1/2MS(大量元素减半,其它成分不变) IBA0.1mg/L 活性碳 0.1%。     

美洲南蛇藤的组织培养与快速繁殖

1 植物名称美洲南蛇藤(Celastrus scandens L.). 2 材料类别幼嫩的顶芽或带腋芽的茎段. 3 培养条件(1)芽诱导培养基:1/3MS+6-BA 1.0mg·L-1(单位下同)+IBA 0.02+3%蔗糖;(2)增殖培养基:MS+6-BA 1.0+IBA 0.2+3%蔗糖;(3)壮苗培养基:MS+6-BA 0.1+IBA 0.02+3%蔗糖;(4)生根培养基:1/2MS+NAA 0.5+1.5%蔗糖.     

爱沙木的组织培养和快速繁殖

1植物名称爱沙木(Eremophila bignoniiflora). 2材料类别无菌萌发的无根种子苗. 3培养条件种子萌发培养基:(1)MS 6-BA 1.0mg·L-1(单位下同) NAA 0.1.分化培养基:(2)MS 6-BA 2.0 NAA 0.1;(3)MS 6-BA 0.5 NAA0.1;(4)MS 6-BA 2.0 IBA 0.01;(5)MS 6-BA0.5 IBA 0.01.壮苗培养基:(6)MS.生根培养基:(7)1/2MS NAA 0.1 1%活性炭;(8)1/2MS NAA0.2 1%活性炭;(9)1/2MS IBA 0.01 1%活性炭;(10)1/2MS IBA 0.02 1%活性炭.以上除生根培养基加入20g·L-1蔗糖外均加入30 g·L-1蔗糖、7g·L-1琼脂,pH 5.6～5.8.光照12 h·d-1,光照度1 500～2000 lx,培养温度23～25℃.     

白玉兰的组织培养和快速繁殖

1植物名称白玉兰(Magnolia denudata). 2材料类别休眠顶芽. 3培养条件芽诱导增殖培养基:(1)MS 6-BA 0.2mg·L-1(单位下同) NAA 0.05,(2)MS 6-BA0.5 NAA 0.05,(3)MS 6-BA 1.0 NAA 0.05,(4)MS 6-BA 0.2 NAA 0.1,(5)MS 6-BA 0.5 NAA0.1,(6)MS 6-BA 1.0 NAA 0.1,(7)MS 6-BA2.0 NAA 0.05;诱导生根培养基:(8)1/2MS IBA0.2,(9)1/4MS IBA 0.5.以上培养基加0.7%琼脂、100mg·L-1肌醇,蔗糖除生根培养基为20 g·L-1外其余均为30 g·L-1,pH 5.8.培养温度20～28℃,光照度1 500～2500 lx,光照时间9～11 h·d-1.     

尾叶桉叶片的离体培养和植株再生

1植物名称尾叶桉(Eucalyptus urophylia). 2材料类别无菌萌发种子苗的幼叶片. 3培养条件萌发培养基:(1)MS 3%蔗糖.诱导分化培养基:(2)MS 6-BA 0.8～1.5 mg·L-1(单位下同) KT 0.5 NAA 0.1 3%蔗糖.丛生芽增殖和生长培养基:(3)MS 6-BA 0.2 NAA 0.02 3%蔗糖.生根培养基:(4)1/2MS NAA 0.5 2%蔗糖;(5)MS NAA 0.5 2%蔗糖;(6)改良H NAA 0.5 2%蔗糖.以上培养基均加入0.65%琼脂,pH 5.8.培养温度(25±2)℃,光照1 6 h·d-1,光照度1900～2000lx.     

喜树不定芽的诱导及植株再生

1植物名称喜树(Camptotheca acuminate),别名旱莲木. 2材料类别一年生喜树带芽茎段. 3培养条件芽诱导培养基:(1)MS NAA 0.05mg·L-1(单位下同) 6-BA 1.0.芽增殖培养基:(2)MS NAA 0.1 6-BA 0.5;(3)MS NAA 0.1 6-BA 1.0;(4)MS NAA 0.1 6-BA 2.0;(5)MS NAA0.5 6-BA 0.5;(6)MS NAA 0.5 6-BA 1.0;(7)MS NAA 0.5 6-BA 2.0.壮苗培养基:(8)MS NAA 0.05 6-BA 0.1～0.5.生根培养基:(9)1/2MS NAA 0.1 IBA 1.0;(10)1/2MS NAA 0.5 IBA1.0;(11)1/2MS NAA 1.0 IBA 1.0.以上所有培养基均附加6.5 g·L-1琼脂、30 g·L-1蔗糖,pH值为5.9.培养温度为(25±2)℃,光照度为1000～2000lx,光照时间14 h·d-1.     

光叶楮的组织培养和快速繁殖

1植物名称光叶楮(Broussonetia papyrifera),又名构树. 2材料类别茎尖与茎段. 3培养条件启动培养基:(1)MS 6-BA 1.0 mg·L-1(单位下同) NAA 0.5.增殖培养基:(2)MS 6-BA0.5 NAA 0.02;(3)MS 6-BA 1.0 NAA 0.2;(4)MS 6-BA 1.5 NAA 0.1;(5)MS 6-BA 1.5 NAA 0.5.生根培养基:(6)1/2MS IBA 0.5;(7)1/2MS NAA0.3;(8)MS 6-BA 0.2 NAA 0.01.以上培养基均添加3%蔗糖、0.7%琼脂,pH 5.8.培养温度25～28℃,光照12 h·d-1,光照度2 000lx.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.