竹红菌甲素对红细胞膜内脂双层的微扰

In this paper, using human erythrocyte membrane, the effect of Hypocrellin A on the lipid bilayer of the membrane was studied by measuring the change of the fluidity of the membrane, the energy transfer of the fluorescent probes, the shift of the fluorescent emission peaks, and the split of band-a of Hypocrellin A. The results showed that in the presence of HA, the fluidity of erythrocyte membrane was increased, the fluorescence intensity of the probes was decreased, and the fluorescence peaks shifted blue. These phenomena took place more seriously with the increment of HA concentration. Meanwhile, the band-a of HA excitation spectra was splitted. It was suggested from all of the results that HA could significantly perturb the lipid bilayer of erythrocyte membrane, there were interactions existing between the Hypocrellin A and the membrane. The HA was mainly located in the middle range of the membrane lipid bilayer when in high concentration (mainly to the 12-16 positions of the long chain fatty acid).     

竹红菌甲素对红细胞膜和几种磷脂脂质体膜的流动...

In this paper, the photodamage of Hypocrellin A to the fluidity of human erythrocyte membranes and some kinds of membranes of phospholipid liposomes was investigated by measuring the changes in fluorescence polarization of the membranes. The results showed that the photosensitization effect of HA caused the decrease of membrane fluidity of the phospholipid (DPPC, DPPC/DPPE, phospholipid of erythrocyte membranes) liposomes. The DPPC and DPPC/DPPE liposomes were more sensitive to the damage than the phospholipid liposomes of erythrocyte membranes. To human erythrocyte membranes, the photodamage effect of HA caused its fluidity first increased and then, with the increment of illumination time, decreased. To spectrin-depleted and trypsin-treated erythrocyte membranes, this kind of change in fluidity was inhibited. All of the results indicated that phospholipids and proteins play different roles in the photodamage of HA to the fluidity of membranes. Membrane proteins, especially spectrin, were the key factor involved in the changes of the fluidity.     

用稳态荧光探针研究竹红菌乙素光敏损伤对人红细胞膜流动性的影响

本文以红细胞膜为材料,用了三种稳态荧光探针研究了HB光敏作用引起人红细胞膜流动性的改变.实验结果表明在HB光敏作用下,膜的旋转扩散速度和侧向扩散速度均发生明显变化,ANS和DPH探针测得HB引起膜流动性降低,也就是膜粘度增加,用芘探针结果则表明膜的侧向扩散变慢.本文还对HB光敏作用的机理进行了探讨,我们观察了数种单重态氧猝灭剂,羟自山基猝灭剂和抗氧化剂对于光敏作用的影响,分别测定了膜流动性和膜的内源荧光的变化,发现在HB光敏作用中,除了~1O_2的作用之外,还存在其它自由基的作用.在HB与HA光敏能力的比较中发现,在比较高一些浓度条件下,存在着HB大于HA的趋向.     

反相薄层色谱扫描方法分离蟾酥成分及测定脂蟾毒配基含量

本文用 BC_(18)F_(254)板,乙腈-水(1:1)作展开剂,展开8cm。反相薄层色谱扫描法分离并检测蟾酥中的八个成分,其中脂蟾毒配基的定性、定量方法已被建立。并用此法测定了三个蟾酥样品中脂蟾毒配基的含量。平均回收率为103.8％,变异系数(CV)小于2％,比现有其它方法重视性好,分离效率高,操作简便。     

神经元营养因子对大鼠隔—海马通路损伤的影响

为评价神经生长因子(NGF)、混合型神经节苷脂(GM)和单唾液酸神经节苷脂(GM1)对中枢胆碱能神经损伤早期的影响,在大鼠单侧隔-海马通路部分损伤后即时经脑室分别注入上述三种神经元营养因子,7d后取两侧海马分别测定乙酰胆碱(ACh)、胆碱乙酰基转移酶(ChAT)和胆碱酯酶(ChE)。损伤对照组(脑室注入盐水)术侧海马ACh含量保留率为对侧的20.3％,ChAT活力为50％,ChE活力为48.3％。给予NGF、GM或GM1的实验组,ACh含量保留率分别为34.9％,35.3％和47.7％;ChAT活力为77.4％,78.4％和69.2％;而ChE活力的保留率未见明显改变。这些神经元营养因子显著增加了大鼠隔-海马通路损伤后海马内ACh含量和ChAT活力,说明它们减轻了损伤侧海马胆碱能神经纤维的破坏,具有明显的损伤早期保护作用。     

羟基自由基对兔脑微粒体膜脂及膜蛋白的损伤

本文研究了过氧化氢与亚铁离子体系产生的羟基自由基对兔脑微粒体脂质过氧化作用及对膜上(Na~++K~+)-ATP酶活性的影响.结果表明,羟基自由基导致兔脑微粒体脂质过氧化,增加丙二醛的含量.羟基自由基还使微粒体膜巯基数下降,(Na~++K~+)-ATP酶活力受到抑制.阿魏酸钠对抑制微粒体脂质过氧化及对膜巯基和(Na~++K~+)-ATP酶均有保护作用.自旋捕集实验结果进一步证明药物对羟基自由基的猝灭作用.     

浑球红假单胞菌氨同化途径的研究

本文测定了浑球红假单胞菌(Rhodobacter sphaeroides)菌株601谷氨酰胺合成酶(GS)、谷氨酸合酶(GOGAT)、谷氨酸脱氢酶(GDH)和丙氨酸脱氢酶(ADH)的活性。低氨时,GS/GOGAT活力高,GDH活力低,高氨时,GS/GOGAT活力低,GDH活力高。在以分子氮或低浓度氨为氮源的培养条件下,加入GS抑制刑MSX(L—methionine—DL—sulphoximine),细菌生长受到抑制。但是,生长在以谷氨酸为氮源的细菌则不受影响。上述结果表明,浑球红假单胞菌菌株601氨同化是通过GS/GOGAT途径和GDH途径。     

槟榔红脉穗螟的生物学特性及其防治

本文描述了红脉穗螟的形态特征、生活习性、为害特点、世代和生活史。该虫在海南省兴隆地区1年发生8～9代。为害槟榔的重要时期是佛焰苞开放前期和幼果至中果期。应用呋喃丹与肥料混合1次根区处理及苏云金杆菌与苦楝油喷雾均获得满意的治虫增产效果。