神经元营养因子对大鼠隔—海马通路损伤的影响

为评价神经生长因子(NGF)、混合型神经节苷脂(GM)和单唾液酸神经节苷脂(GM1)对中枢胆碱能神经损伤早期的影响,在大鼠单侧隔-海马通路部分损伤后即时经脑室分别注入上述三种神经元营养因子,7d后取两侧海马分别测定乙酰胆碱(ACh)、胆碱乙酰基转移酶(ChAT)和胆碱酯酶(ChE)。损伤对照组(脑室注入盐水)术侧海马ACh含量保留率为对侧的20.3％,ChAT活力为50％,ChE活力为48.3％。给予NGF、GM或GM1的实验组,ACh含量保留率分别为34.9％,35.3％和47.7％;ChAT活力为77.4％,78.4％和69.2％;而ChE活力的保留率未见明显改变。这些神经元营养因子显著增加了大鼠隔-海马通路损伤后海马内ACh含量和ChAT活力,说明它们减轻了损伤侧海马胆碱能神经纤维的破坏,具有明显的损伤早期保护作用。

EFFECTS OF NEURONOTROPHIC FACTORS ON LESIONS OF SEPTO-HIPPOCAMPAL PATHWAY IN RATS

Effects of intraventricular administration of nerve growth factor (NGF), ganglioside mixture (GM) or monosialoganglioside (GMl) on changes of acetylcholine (ACh), choline acetyltransferase (ChAT) and cholinesterase (ChE) in the hippocampus of rats with unilateral partial septo-hippocampal transection were studied. The neurotrophic factors were injected at 0 and 30 minutes after lesion. On the 7th day after the lesion, biochemical assays confirmed that all the three neurotrophic factors did not increase ChE activity in the denervated hippocampus. ACh contents in the ipsilateral hippocampus retained 34.9%, 35.3% and 47.7% respectively in NGF, GM and GMl treated rats, whereas it was 20.3% in lesioned controls. ChAT activity retained 77.4%, 78.4% and 69.2% respectively, whereas it was 50% in lesioned controls.All the three neurotrophic factors induced increases in ACh content and ChAT activity in the hippocampus of the lesioned side, suggesting that treatments with NGF, GM and GM 1 have protective effects at early stage of rat septo-hippocampal lesion.