全文获取类型
收费全文 | 127篇 |
免费 | 8篇 |
国内免费 | 14篇 |
出版年
2023年 | 3篇 |
2022年 | 4篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 2篇 |
2014年 | 6篇 |
2013年 | 10篇 |
2012年 | 3篇 |
2011年 | 5篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 9篇 |
2007年 | 3篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 12篇 |
2003年 | 10篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 3篇 |
排序方式: 共有149条查询结果,搜索用时 15 毫秒
21.
Persistence of conidia of an isolate of Erynia radicans (Syn. Zoophthora radicans) was investigated in relation to the meteorological conditions which occurred during autumn-winter of 1990–91 in the coastal plain in Israel. Capilljconidia shielded from the sun, placed on the abaxial surface of leaves of Plumeria acuminata, persisted for 24 h to at least 120 h. Exposed capilliconidia, placed on the adaxial surface of the same leaves, died within 24 h. Almost all the primary conidia shielded from the sun (placed on the abaxial surface of the same leaves) died within a single day. Conidial viability was expressed in subsequent germination on an agar medium. Capilliconidial persistence was closely related to the daily air temperatures, expressed as cumulative day-degrees. Differences in relative humidity had no substantial effect on capilliconidial mortality. At daytime temperatures of ≤ 20°C, mortality after 24 h incubation was lowest (≤ 34%) and the persistence duration, longest (at least 120 h). Increases in daytime temperature up to 24°C for a few hours increased mortality (37–57% after 24 h incubation) and shortened the persistence duration (72–120 h). Exposure to 24–29°C during daytime greatly increased mortality (65–58% after 24 h) and further shortened the persistence duration (24–48 h). Daytime temperatures of > 29°C were lethal to all capilliconidia within 24 h. Temperature had a profound effect on capilliconidial persistence also under controlled environmental conditions. The significance of capiliiconidial persistence is discussed in relation to activity of the fungus in its natural environment. 相似文献
22.
Fabio Ribeiro Braga Jackson Victor Araújo Filippe Elias de Freitas Soares Juliana Milani Araujo Sebastião Rodrigo Ferreira José Humberto de Queiroz 《Biocontrol Science and Technology》2012,22(5):559-565
The objective of the present work was to study the conditions of predation of Duddingtonia flagrans conidia versus Panagrellus sp using response surface methodology. Conidia of D. flagrans (AC001) isolate were transferred into water-agar (WA) culture media at different pHs and different concentrations defined according to Central Composite Design (CCD). Five different concentrations of D. flagrans conidia: (1292, 500, 1000, 1500 and 1707) were used. For 2%WA media were used the following pHs were used: 6.29, 6.5, 7.0, 7.5 and 7.71. The response of the design corresponded to the number of larvae (transformed to square root scale) observed at the end of the experiment (10 days). At the tenth day, the non predated larvae were recovered in the Petri dishes. The results showed that 2%WA media at pH 7.0 contributed to improve the predatory activity of conidia of D. flagrans, and therefore this tool may be used in future studies under laboratory and natural conditions. 相似文献
23.
通过电子显微镜和细胞化学标记研究了哈氏木霉分生孢子发育的超微结构和细胞化学。分生孢子发育的超微结构研究表明,分生孢子壁的发育是有个由薄而光滑到厚而有疣的过程;期间脂肪体在分生孢子和产孢细胞中不断累积,最后脂肪体沿着内壁排列成一层。免疫金标记结果显示,幼嫩的分生孢子壁中缺乏几丁质和纤维素,只有在成熟的分生孢子壁中含有几丁质;出乎意料的是在成熟分生孢子中发现有少量纤维素的存在。 相似文献
24.
Beauveria bassiana has a high insecticidal potential to control the tarnished plant bug, Lygus lineolaris, a significant pest of strawberries. Screening experiments showed that L. lineolaris adults were susceptible to several B. bassiana isolates. Another screening test with Coleomegilla maculata, a natural enemy found in strawberries, was also performed in order to select the isolate having lower entomopathogenic impact on this insect. Based on data obtained from both insect species and on the ecozone origin of the B. bassiana isolates, INRS‐IP and INRS‐CFL isolates were selected for further experiments. The LC50 values of these two isolates against L. lineolaris adults were 7.8 × 105 and 5.3 × 105 conidia/ml, and average survival time (AST) values were 4.46 and 4.37 days at a concentration of 1 × 108 conidia/ml respectively. Results also indicated that L. lineolaris nymphs are susceptible to the selected isolates. During field experiments, using a randomized block design with four replicates, INRS‐IP and INRS‐CFL isolates were applied at two rates (1 × 1011 and 1 × 1013 conidia/ha) weekly during a period of 4 weeks. These multiple applications triggered a significant reduction of L. lineolaris nymphal populations in strawberries. Twenty‐four days after the first application, a significant difference was observed between the mean population densities of surviving nymphs in all B. bassiana‐treated plots (less than one insect per five plants) compared with those in control plots (four insects per five plants). During the field experiment, persistence of insecticidal activity and viability of B. bassiana conidia were also monitored. The results showed the presence of viable and infective conidia up to 6 days after each application on strawberry foliage. Moreover, the multiple applications of B. bassiana at the rate of 1 × 1013 conidia/ha triggered a significant reduction in strawberry fruit injuries induced by L. lineolaris feeding behaviour compared with the control plots. 相似文献
25.
Identification of Botrytis spp. on Plants Grown in Iran 总被引:1,自引:0,他引:1
S. Mirzaei E. Mohammadi Goltapeh M. Shams-Bakhsh N. Safaie 《Journal of Phytopathology》2008,156(1):21-28
A total of 363 isolates were collected from all over Iran. They were isolated from apple, arum lily, briar rose, bride wort, broad bean, camellia, canola, carnation, cucumber, egg plant, feijoa, geranium, gerbera, gladiolus, grape, guilder rose, hibiscus, iris, kiwifruit, oleander, onion, orange, pear, pomegranate, primrose, quince, redbud, robinia, rose, rubber plant, sow thiste, spathe flower, strawberry, tomato, violet, wall flower and wheat. To identify the species, morphological characters such as conidiophore length, conidial and sclerotial dimensions were measured. According to morphological and cultural characters, eight Botrytis species were identified: B. aclada sensu lato, B. cinerea, B. fabae, B. convoluta, B. gladiolorum, B. paeoniae, B. pelargonii and B. porri. As far as we are aware, this is the first report of the last five species from Iran. These species were examined by polymerase chain reaction (PCR) using necrosis and ethylene‐inducing protein (NEP2) and C729 primers. A 835 bp band was amplified in B. cinerea, B. fabae and B. pelargonii, using NEP2, but not in others. However, C729 primers amplified a 700 bp band in B. cinerea and B. pelargonii and a 600 bp in B. fabae. 相似文献
26.
Myxotrichum albicans,a new slowly-growing species isolated from forest litters in China 总被引:2,自引:0,他引:2
Myxotrichum albicans sp. nov. is a slowly-growing fungal species that was isolated from forest litters in northeast of China. The new species is morphologically characterized by its white colonies on CMA, PCA, PDA, and YMA, thallic conidiogenous cells, simple, catenate and hyaline conidia. Phylogenetic analysis based on internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequences also supported the placement of M. albicans as a new species in the genus Myxotrichum. To compare with related species, the optimal pH and temperature for growth were investigated. The differences among Myxotrichum (Oidiodendron) species producing hyaline conidia were discussed. 相似文献
27.
Diana Tamayo Orville Hernández Cesar Mu?oz-Cadavid Luz Elena Cano Angel González 《Memórias do Instituto Oswaldo Cruz》2013,108(4):488-493
The infectious process starts with an initial contact between pathogen
and host. We have previously demonstrated that Paracoccidioides
brasiliensis conidia interact with plasma proteins including
fibrinogen, which is considered the major component of the coagulation system.
In this study, we evaluated the in vitro capacity of P.
brasiliensis conidia to aggregate with plasma proteins and
compounds involved in the coagulation system. We assessed the aggregation of
P. brasiliensis conidia after incubation with human serum
or plasma in the presence or absence of anticoagulants, extracellular matrix
(ECM) proteins, metabolic and protein inhibitors, monosaccharides and other
compounds. Additionally, prothrombin and partial thromboplastin times were
determined after the interaction of P. brasiliensis conidia
with human plasma. ECM proteins, monosaccharides and human plasma significantly
induced P. brasiliensis conidial aggregation; however,
anticoagulants and metabolic and protein inhibitors diminished the aggregation
process. The extrinsic coagulation pathway was not affected by the interaction
between P. brasiliensis conidia and plasma proteins, while the
intrinsic pathway was markedly altered. These results indicate that P.
brasiliensis conidia interact with proteins involved in the
coagulation system. This interaction may play an important role in the initial
inflammatory response, as well as fungal disease progression caused by
P. brasiliensis dissemination. 相似文献
28.
Indirect evidence for the natural existence of the free-protoplast stage of the fungus Entomophthora egressa in the eastern hemlock looper, Lambdina fiscellaria fiscellaria, is presented. The protoplasts were viable after 72 hr postinjection and subsequent development in the host produced conidia characteristic of E. egressa. The hemocytes studied (plasmatocytes, granular cells, and spherule cells) did not adhere to the protoplasts either in vivo or in vitro. Cells of Escherichia coli and sporangiospores of Absidia repens adhered to the granular cells in vitro. The granular cells adhered to the hyphae of Rhizopus nigricans in vitro. The spherule cells strongly adhered to the hyphae and hyphal bodies of E. egressa in vitro. The protoplasts, hyphae, and conidia of E. egressa and the hemocytes of L. fiscellaria fiscellaria adhered to positively charged DEAE-Sephadex beads and not to negatively charged CM-Sephadex beads. Aspects of active and passive strategies for protoplast evasion of host hemocytes are discussed with some emphasis on hemocyte-protoplast electrostatic repulsion and active secretion of hemocyte inhibitors by the protoplasts. 相似文献
29.
30.