哈氏木霉分生孢子发育的超微结构和细胞化学

通过电子显微镜和细胞化学标记研究了哈氏木霉分生孢子发育的超微结构和细胞化学。分生孢子发育的超微结构研究表明,分生孢子壁的发育是有个由薄而光滑到厚而有疣的过程;期间脂肪体在分生孢子和产孢细胞中不断累积,最后脂肪体沿着内壁排列成一层。免疫金标记结果显示,幼嫩的分生孢子壁中缺乏几丁质和纤维素,只有在成熟的分生孢子壁中含有几丁质;出乎意料的是在成熟分生孢子中发现有少量纤维素的存在。

ULTRASTRUCTURE AND CYTOCHEMISTRY OF THE CONIDIAL DEVELOPMENT OF TRICHODERMA HARZIANUM

Ultrastructure and cytochemistry of the conidial development of Trichoderma harzianum were investigated. The results showed that conidia were thin -walled and smooth to thick walled and verrucose; fat bodies accumulated gradually in both conidium and conidiogenous cell, and finally laid over the inner wall of conidium and disappeared in conidiogenous cell. Immunogold labeling demonstrated that there was no chitin and cellulose in young and thin conidial walls; chitin appeared in the well-developed wall in later stage of conidial development and little cellulose, however, was detectable in the wall of mature conidia.