抗金环蛇毒血清生产工艺的研究

目的研制抗金环蛇毒血清,以解决被金环蛇咬伤后的救治问题。方法以金环蛇毒为免疫原免疫马获得免疫血浆,经盐析制备IgG,IgG再经酶解、疏水柱纯化后得到F(ab′)2活性片段,并以免疫扩散、ELISA测定其抗体特异性,以小鼠试验法测定其中和金环蛇毒能力。结果得到的抗血清F(ab′)2冻干品纯度达90%以上。结论研制出了特异性强、效价高的抗金环蛇毒血清。     

凝血酶冻干制剂冻干工艺选择研究

在对凝血酶精制、制剂配制、稳定剂选择和冻干工艺试验、冻干制剂稳定性等进行了一系列的试验之后,建立了凝血酶冻干制剂的活性单位检测方法,得到了凝血酶冻干制剂(速效局部止血药200 IU/m l和500 IU/m l)的试制结果。在选定的制造工艺条件下,连续生产三批,经甘肃省药品检验所检定结果,无菌试验合格,单位含量合格并相当于标示量的100%~140%,比活高于10 IU/mg,残余水份含量低于3%,制剂质量均达到2005版药典标准的要求。凝血酶冻干制剂的稳定性良好,37℃保存三个月后标示量下降在10%以内。     

克鲁维酵母高渗培养与原生质体制备与再生

研究了高渗预培养条件对克鲁维酵母Ｙ０３４原生质体形成与再生的影响。结果表明,同等条件下,经高渗预培养的原生质体再生率为常规培养的１．７倍,为改造菌株遗传特性而制备高活性原生质体探索了新的途径。     

Application of CdS quantum dots modified carbon paste electrode for monitoring the process of acetaminophen preparation

In this research article, a novel, selective, and sensitive modified carbon paste electrode (CPE) using CdS quantum dots (QDs) is presented. The highly stable CdS QDs were successfully synthesized in an in situ process using Na₂S₂O₃ as a precursor and thioglycolic acid as a catalyst and capping agent. The synthesis of CdS QDs was studied using X-ray diffraction (XRD) and transmission electron microscopy (TEM) techniques. The synthesized CdS QDs were used for preparation of a modified carbon paste electrode (CdS/CPE). The electrochemical behavior of the electrode toward p-aminophenol (PAP) and acetaminophen (Ac) was studied, and the results demonstrated that the CdS/CPE exhibited good electrocatalytic performance toward PAP and Ac oxidation. The oxidation peak potential of each analyte in the mixture was well separated. As a result, a selective and reliable method was developed for the determination of PAP and Ac simultaneously without any chemical separations. Application of the fabricated electrode for monitoring the process of Ac preparation from PAP was investigated. The obtained results show that CdS/CPE has satisfactory analytical performance; it could be a kind of attractive and promising nanomaterial-based sensor for process monitoring via the electrochemical approach.     

基于专利分析的天然抗肿瘤药制剂技术发展态势研究

目的:基于专利文献深入分析天然抗肿瘤药制剂技术的发展态势。方法:采用专利计量分析、统计学趋势分析等定量分析方法结合药学专业知识的定性分析方法从专利申请数量、国家/地区分布、专利技术领域等方面对天然抗肿瘤药制剂技术领域的发展态势进行分析。结果:天然抗肿瘤药制剂技术专利年申请数量在3000件左右,处于比较稳定平稳的发展时期;专利申请主要集中在美国和中国;专利技术领域主要集中在抗肿瘤活性和剂型的相关研究。结论:美国专利申请数量排名第一,远远领先于其他国家,且专利质量很高,处于绝对的领先地位。中国虽然在专利申请数量方面具有一定优势,但是专利质量亟待提高,与国外发达国家相比仍有较大差距。     

反相高效液相色谱法制备HMC毒素纯品

本文采用反相高效液相色谱纯化制备了从玉米小斑病病菌Ｃ小种（ＨｅｌｍｉｎｔｈｏｓｐｒｉｕｍｍａｙｄｉｓＲａｃｅＣ）分离出来的毒素ＴｏｘｉｎＩ,经ＮＭＲ的结构分析证实此制备物纯度较高,达到结构分析要求。用氯仿提取含有ＨＭＣ的毒素培养滤液,经过多次ＴＬＣ展层层析（ＣＨＣｌ_３：ＭｅＯＨ＝９：１）,刮取具有侵染活性的部分（Ｒ_ｆ＝０．４）溶解在小体积甲醇中备用。色谱柱为Ｗａｔｅｒｓ（制备型２５０ｍｍ×１０ｍｍ）填料为ＹＷＧ－Ｃ１８反相柱,粒度１０ｕｍ,流动相选用甲醇：水５５：４５     

Effect of microencapsulated cell preparation technology and conditions on the catalytic performance of Penicillium purpurogenum Li-3 strain cells

Microencapsulated cell preparation technology was applied to the hydrolysis of glycyrrhizin (GL) with Penicillium purpurogenum Li-3 whole-cell catalytic technology into glycyrrhetinic acid monoglucuronide (GAMG) possessing better bioavailability, sweetness, and security. The effect of the key techniques and technological conditions used for preparation of alginate–chitosan microencapsulated P. purpurogenum Li-3 strain cell on its usability was investigated. Results showed that technological conditions were crucial for microencapsulated cells to play the best. After cells were microencapsulated, mass transfer efficiency decreased slightly, resulting in a small decrease in catalytic activity. However, the cells obtained valid protection following microencapsulation, and thus exhibited better growth. Moreover, after continuous use for 12 batch cycles, 50.11% residual activity of the microencapsulated cells remained, and the breakage rate of microcapsules was only 6.4%. Therefore, microencapsulated P. purpurogenum Li-3 strain cells possessed comparatively high mechanical strength and stability.     

Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate

The preparation of 1-deoxy-d-xylulose 5-phosphate, the key intermediate of MEP biosynthetic pathway for terpenoids by using recombinant 1-deoxy-d-xylulose 5-phosphate synthase of Rhodobacter capsulatus was optimized. The simple one-pot synthesis coupling with a newly established ion-exchange purification process affords the target compound with more than 80% yield and high purity (>95%). The procedure can also be employed to synthesize isotope labeled 1-deoxy-d-xylulose 5-phosphate by using isotope labeled starting materials.     

Preparation of a series of chitooligomers and their effect on hepatocytes

A series of chitooligomers with molecular weight ranging from 1.7  3.8 × 10³ were prepared by degradation of a high molecular weight chitosan with hydrogen peroxide and selective precipitation in ethanol solutions. The prepared chitooligomers were characterized by gel permeation chromatography, elemental analysis, Fourier transform infrared spectra, ¹³C nuclear magnetic resonance spectroscopy and X-ray diffraction analysis. Cell culture experiments suggested that the effect of the chitooligomers on the proliferation of L02 hepatocytes was dependent on culture time, namely, at the initial stage of culture there was an inhibitory effect on proliferation of the cells; however, the cultures recovered in cell proliferation and exhibited promotion effect in following days. In the case of chitosan monomer (GlcN), high concentration of GlcN (1 mg/ml) produced a significant suppression in proliferation of L02 cells relative to control, with decreases of 35.2%, 60% and 72.9% on days 1, 3 and 5, respectively. In addition, there was no significant effect of the chitooligomers on the functions of albumin secretion and urea synthesis of the hepatocytes.