MTHFR基因多态性与动脉粥样硬化性脑梗塞的关系

采用PCR-RFLP技术,检测了62例动脉粥样硬化性脑梗塞患者和79名对照者的C677T突变的基因型。结果发现, MTHFR基因C677T 突变型等位基因(V)频率在实验组和对照组中,有显著性差异(χ2＝4.41,P<0.05);三种基因型频率在两组人群中均无显著性差异。基因型频率的相对风险分析,AV基因型比AA基因型患脑梗塞风险高1.76倍;VV基因型比AA基因型患脑梗塞风险高3.25倍。结果表明, MTHFR基因C677T突变型等位基因与动脉粥样硬化性脑梗塞有一定的关联,突变基因型增加了动脉粥样硬化脑梗塞的发病风险。 Abstract:　In order to detect the relationship between MTHFRgene C677Tpolymorphism and arteriosclerotic cerebral infarction, this study examined the genotype of 62 patients with arteriosclerotic cerebral infarction and 79 control subjects by PCR-RFLP. The result showed that there was significance difference between patients and control subjects in V allele frequency of MTHFRgene C677Tmutation (χ2＝4.41,P<0.05) and there was no difference between patients and control subjects in genotype frequency of MTHFRgene C677Tmutation. The relative risk for arteriosclerotic cerebral infaction of heterozygote (AV/AA) was 1.76 and that of homozygote (VV/AA) was 3.25. The study confirmed an association between mutated allele of the MTHFRgene C677Tand arteriosclerotic cerebral infarction, mutated genotypes increased the risk of arteriosclerotic cerebral infarction.     

中国蕨类植物学的奠基人秦仁昌(1898～1986)——纪念秦仁昌先生诞辰一百周年

中国蕨类植物学的奠基人秦仁昌（１８９８～１９８６）———纪念秦仁昌先生诞辰一百周年王中仁（中国科学院植物研究所系统与进化植物学开放研究实验室北京１０００９３）ＴＯＣＯＭＭＥＭＯＲＡＴＥＴＨＥ１００ＴＨＡＮＮＩＶＥＲＳＡＲＹＯＦＴＨＥＢＩＲＴＨＯＦＣＨ...     

植物遗传多样性研究中等位基因酶分析的遗传参数及其计算方法（综述）

植物遗传多样性研究中等位基因酶分析的遗传参数及其计算方法（综述）葛学军（中国科学院华南植物研究所,广州５１０６５０）ＴＨＥＧＥＮＥＴＩＣＰＡＲＡＭＥＴＥＲＳＡＮＤＴＨＥＩＲＣＡＬＣＵＬＡＴＩＮＧＭＥＴＨＯＤＳＯＦＡＬＬＯＺＹＭＥＡＮＡＬＹＳＩＳＩＮＰ...     

根田鼠肾上腺皮质酮水平的日节律及急性低氧的影响

根田鼠肾上腺皮质酮水平的日节律及急性低氧的影响ＴＨＥＣＩＲＣＡＤＩＡＮＲＨＹＴＨＭＯＦＣＯＲＴＩＣＯＳＴＥＲＯＮＥＬＥＶＥＬＡＮＤＥＦＦＥＣＴＯＦＨＹＰＯＸＩＡＯＮＲＯＯＴＶＯＬＥ（ＭＩＣＲＯＴＵＳＯＥＣＯＮＯＭＵＳ）根田鼠（Ｍｉｃｒｏｔｕｓｏｅｃ...     

THE DEVELOPMENT OF MOLECULAR ECOLOGY FORM MOLECULAR BIOLOGY IS AN EXAMPLE OF CONCEPT CHANGE

ＴＨＥＤＥＶＥＬＯＰＭＥＮＴＯＦＭＯＬＥＣＵＬＡＲＥＣＯＬＯＧＹＦＯＲＭＭＯＬＥＣＵＬＡＲＢＩＯＬＯＧＹＩＳＡＮＥＸＡＭＰＬＥＯＦＣＯＮＣＥＰＴＣＨＡＮＧＥＸｉａｎｇＪＭ,ＬｉｎＹＬ,ＬｉｕＸＦ,ＬｉＨＶＩＲＵＳＲＥＳＥＡＲＣＨＩＮＳＴＩＴＵＴＥＡＮ...     

鳙团移核鱼的遗传性状与个体生长

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中国科学院动物研究所兽类标本馆藏模式标本名录

中国科学院动物研究所兽类标本馆藏模式标本名录ＣＡＴＡＬＯＧＵＥＯＦＴＨＥＭＡＭＭＡＬＴＹＰＥＳＰＥＣＩＭＥＮＳＰＲＥＳＥＲＶＥＤＩＮＴＨＥＭＡＭＭＡＬＣＯＬＬＥＣＴＩＯＮＯＦＴＨＥＩＮＳＴＩＴＵＴＥＯＦＺＯＯＬＯＧＹ,ＣＨＩＮＥＳＥＡＣＡＤＥＭＹＯＦ...     

六出花切花的瓶插花期延长

六出花切花的瓶插花期延长李宪章（中国科学院植物研究所,北京１０００９３）ＬＩＦＥＥＸＴＥＮＳＩＯＮＯＦＴＨＥＣＵＴＦＬＯＷＥＲＯＦＡＬＳＴＲＯＭＥＲＩＡＡＵＲＡＮＴＩＡＣＡＷＩＴＨＦＬＯＲＡＬＰＲＥＳＥＲＶＡＴＩＶＥＳＬｉＸｉａｎｚｈａｎｇ（Ｉ...     

鲇卵子发生中卵膜的形成

鲇卵子发生中卵膜的形成魏刚,戴大临,陈怀辉（西南师范大学生物系,重庆６３０７１５）关键词鲇,卵母细胞膜,卵子发生ＴＨＥＦＯＲＭＡＴＩＯＮＯＦＴＨＥＯＯＣＹＴＥＥＮＶＥＬＯＰＥＩＮＴＨＥＯＯＧＥＮＥＳＩＳＯＦＴＨＥＣＡＴＦＩＳＨＳＩＬＵＲＵＳＡＳＯＴＵ...     

太平洋大海洋生态系国际学术讨论会在青岛召开

太平洋大海洋生态系国际学术讨论会在青岛召开Ｉｎｔｅｒｎａｔｉｏｎａｌｓｙｍｐｏｓｉｕｍｏｎ“ＴＨＥＬＡＲＧＥＭＡＲＩＮＥＥＣＯＳＹＳ－ＴＥＭＳ（ＬＭＥｓ）ＯＦＴＨＥＰＡＣＩＦＩＣＯＣＥＡＮ”ｈｅｌｄａｔＱｉｎｇｄａｏ,Ｐ￥Ｒ．Ｃｈｉｎａ太平洋大海洋生...     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.