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1IntroductionElbo(ECG)offersalotofilllcorralltinfondionforthediagnosisOfheartdis-eases.Berz1,seahaormalEChcax[lrins>llleu"knownsituations,thcycanbeCSUghtinthelongti1Ylecontin~11xHlltoriDg.ndterrnoultonngsystemwhichcanrecord24-hoUrECGdataisoneofeffectiveme~toprovidethefun~.AlthOUghthehag6scaleICmorestohavebeeddevelopepbedeavailabletostorelOngtboeECGdsta,itisveqdifficultyandtioublesomethatalopnUmbeOfdstaisprasersandstoredortiallsillltted.InthedigitedECGdata,thereare… 相似文献
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超声彩色血流成像的计算机快速仿真方法 总被引:2,自引:0,他引:2
研究超声彩色血流成像的快速仿真方法,克服原先仿真方法非常耗时的缺点。方法超声彩色血流成像计算机仿真中,血流信号是对成像区间内所有点散射体的回波信号累加而得到的。通过引入新的等效散射体模型,可以大大降低散射体的密度,从而减少计算回波信号所需时间。在计算机上用Matlab编程来进行仿真实验,对以往仿真方法和基于等效散射体模型方法的性能进行比较。结果实验表明:基于等效散射体模型的仿真,在保证相同流速精度的前提下,仿真速度比传统方法提高了10倍以上。结论基于等效散射体模型的仿真方法能极大地提高超声彩色血流成像的仿真速度,可以为超声彩色血流成像的方法研究提供便利。 相似文献
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A highly sensitive and selective optical sensor for Pb(2+) by using conjugated polymers and label-free oligonucleotides 总被引:1,自引:0,他引:1
The detection of Pb(2+) with DNA-based biosensor is usually susceptible to severe interference from Hg(2+) because of the T-Hg(2+)-T interaction between Hg(2+) and T residues. In this study, we developed a rapid, sensitive, selective and label-free sensor for the detection of Pb(2+) in the presence of Hg(2+) based on the Pb(2+)-induced G-quadruplex formation with cationic water-soluble conjugated polymer (PMNT) as a "polymeric stain" to transduce optical signal. We selected a specific sequence oligonucleotide, TBAA (5'-GGAAGGTGTGGAAGG-3'), which can form a G-quadruplex structure upon the addition of Pb(2+). This strategy provided a promising alternative to Pb(2+) determination in the presence of Hg(2+) instead of the universal masking agents of Hg(2+) (such as CN(-), SCN(-)). Based on this observation, a simple "mix-and-detect" optical sensor for the detection of Pb(2+) was proposed due to the distinguishable optical properties of PMNT-ssDNA and PMNT-(G-quadruplex) complexes. By this method, we could identify micromolar Pb(2+) concentrations within 5min even with the naked eye. Furthermore, the detection limit was improved to the nanomolar range by the fluorometric method. We also successfully utilized this biosensor for the determination of Pb(2+) in tap water samples. 相似文献
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Jian Zhang Sha Li Lu Li Meng Li Chongye Guo Jun Yao Shuangli Mi 《基因组蛋白质组与生物信息学报(英文版)》2015,13(1):17-24
Exosomes are 40–100 nm nano-sized vesicles that are released from many cell types into the extracellular space. Such vesicles are widely distributed in various body fluids. Recently,m RNAs and micro RNAs(mi RNAs) have been identified in exosomes, which can be taken up by neighboring or distant cells and subsequently modulate recipient cells. This suggests an active sorting mechanism of exosomal mi RNAs, since the mi RNA profiles of exosomes may differ from those of the parent cells. Exosomal mi RNAs play an important role in disease progression, and can stimulate angiogenesis and facilitate metastasis in cancers. In this review, we will introduce the origin and the trafficking of exosomes between cells, display current research on the sorting mechanism of exosomal mi RNAs, and briefly describe how exosomes and their mi RNAs function in recipient cells.Finally, we will discuss the potential applications of these mi RNA-containing vesicles in clinical settings. 相似文献
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Chitooligosaccharide (COS) or oligochitosan has been shown to induce tobacco defense responses which are connected with nitric
oxide (NO) and OIPK (oligochitosan-induced Ser/Thr protein kinase). The aim of this study was to reveal the relationship between
NO production and OIPK pathway in the defense response of tobacco elicited by COS. NO generation was investigated by epidermal
strip bioassay and fluorophore microscope using fluorophore diaminofluorescein diacetate (DAF-2DA). Tobacco epidermal cells
treated with COS resulted in production of NO, which was first present in chloroplast, then in nucleus, finally in the whole
cell; this NO production was sensitive to NO scavenger cPTIO and the mammalian NO synthase (NOS) inhibitor l-NAME, suggesting that NOS-like enzyme maybe involved in NO generation in tobacco epidermal cells. However, NOS and nitrate
reductase (NR, EC 1.6.6.1) inhibitors reduced NO content in tobacco leaves by using NO Assay Kit, suggesting both NOS and
NR were involved in NO production in tobacco leaves. Using a pharmacological approach and western blotting, we provide evidence
that NO acts upstream of OIPK expression. NO scavenger, NOS inhibitor partly blocked the activation of OIPK and the activities
of several defense-related enzymes induced by COS; treatment with NO donor sodium nitroprusside (SNP) induced the activation
of OIPK and enhanced the defense systems. The results suggest that COS is able to induce NO generation, which results in up-regulation
the activities of some defense-related enzymes through an OIPK-dependent or independent pathway. 相似文献