共查询到20条相似文献,搜索用时 46 毫秒
1.
水稻谷蛋白突变体的筛选及遗传分析 总被引:16,自引:0,他引:16
通过对国内外水稻品种种子的全蛋白分析,筛选到3个谷蛋白突变材料。其中编号W3660种子中37~39kDa与22~23kDa谷蛋白亚基的含量较普通水稻明显降低,而13kDa醇溶蛋白多肽含量则大幅升高;W204和W379种子中37~39kDa与22~23kDa谷蛋白亚基的含量介于普通品种与W3660之间,W379还具超大含量的57kDa多肽,实验证明此多肽属谷蛋白成分。用W3660和普通水稻栽培品种惊人糯(Otorokimochi)构建了杂交群体。后代种子总蛋白SDS-PAGE分析显示,低谷蛋白和高醇溶蛋白性状总是相伴出现;F1种子全部呈现低谷蛋白含量和高醇溶蛋白含量特性;F2种子中呈现低谷蛋白和正常蛋白性状的比例约为3:1;从F3种子分析推断出的F2植株基因型,其低谷蛋白纯合型,杂合型和正常型的分离比例符合1:2:1。表明,W3660的低谷蛋白和高醇溶蛋白性状是由单显性基因控制,而且能稳定地遗传给后代。 相似文献
2.
作物种质资源是品种改良的物质基础,随着分子生物学技术的发展,水稻育种已经发展到分子标记辅助选择与农艺性状选择相结合的高级育种阶段,种质资源的评价必须满足分子辅助选择育种的实际需要,进行分子辅助评价。本研究利用农业部《水稻品种鉴定DNA指纹方法》(NY/T1433-2007)推荐的24对 SSR引物,对32个水稻恢复系和13个水稻保持系资源材料进行DNA多态性分析,利用遗传相似系数及其主要农艺性状的差异选择亲本进行种质创新,在中间世代利用PCR-AccⅠ分子标记进对控制中等直链淀粉含量的基因型进行辅助选择,创制了系列新的优异资源。结果显示,32个水稻恢复系和13个水稻保持系资源材料的遗传相似系数变化范围分别为0.5244~0.8415、0.4783~1,平均遗传相似系数分别为0.6965、0.6542;选用其中的R3076、中恢8006、229和巨风A/B作为亲本,创制了恢复系R7606和细胞质雄性不育系229A系列资源,稻米品质检测结果表明,R7606系列资源有11个品系达到国标二级稻米标准;229A系列资源中则有3个品系达到国标一级,1个品系达到国标二级标准。经田间观察和配组试验,结果显示R7606及229A系列资源农艺性状优良,配合力较好,可以作为杂交水稻育种的优良亲本。本研究还对水稻遗传改良和种质创新过程中,如何利用对水稻优异资源的评价,拓展创新资源的遗传背景和遗传多样性,促进水稻育种和生产可持续发展进行了讨论。 相似文献
3.
功能型稻米因其具有调节人体生理功能、改善人体健康等特点,越来越受到人们的重视。本研究对226份寒地粳稻品种的6种功能营养品质进行了遗传变异、相关分析和主成分分析。结果表明,2年间各功能营养品质差异不显著,赖氨酸、谷蛋白、γ-氨基丁酸、总黄酮含量、平均胚重和胚占糙米重在226份寒地粳稻品种中均呈现较大的变异,其中平均胚重的变异系数最大为92.17%,总黄酮含量的变异系数最小为11.51%。绥粳3号、绥粳5号、牡丹江21号、合粳1号、牡丹江22号、龙糯2号、系选1号7个品种,同时具有2种功能营养品质优良的特点。赖氨酸含量与平均胚重和胚占糙米重呈极显著正相关,总黄酮含量与γ-氨基丁酸含量呈显著负相关,与谷蛋白含量呈显著正相关。胚型因子、黄酮和谷蛋白型因子、γ-氨基丁酸型因子的累计贡献率达到69.149%,是影响寒地粳稻功能营养品质的主要因子。在功能型稻米品种选育过程中,应首先注意对巨胚、高γ-氨基丁酸和低谷蛋白的选择,并可以利用分子标记辅助选择或转基因育种手段,实现多个功能营养品质性状的聚合育种。 相似文献
4.
在云南2种生态条件下,对基因枪法获得的转溶菌酶基因水稻高代品系D2-1-2和受体品种中花9号的农艺性状、稻米蛋白质含量、氨基酸含量和11种矿质元素进行了对照分析。结果表明转基因品系和受体对照在生育期农艺性状上无明显的差异,但转基因水稻结实率和株高明显低于受体品种;转基因水稻糙米蛋白质含量、氨基酸含量以及9种矿质元素含量都有所增加,其中钙、铁和锌等元素增加幅度较大。认为基因枪法转基因植物可能会产生一些潜在的遗传变异,因此,建议应对转基因植物进行多年多点的环境风险评价。 相似文献
5.
6.
7.
金苦荞是近些年创制的苦荞与金荞麦种间杂交形成的双二倍体杂种半多年生新荞麦种类(Fagopyrum tatari-cymosum),为了探讨该荞麦种类的农艺性状和品质性状的遗传规律,以26个高产金苦荞品系为材料,对其品质性状和农艺性状进行了遗传变异研究、相关性分析和聚类分析。结果表明:(1)金苦荞品质性状的变异系数大小表现为醇溶蛋白含量>谷蛋白含量>黄酮含量>总蛋白含量>球蛋白含量>清蛋白含量>淀粉含量。(2)金苦荞农艺性状的变异系数大小表现为主茎分枝数>基部20 cm内节数>主茎粗>籽粒面积>千粒重>主茎节数>株高>籽粒长宽比>籽粒宽>籽粒长>果壳率>籽粒周长>籽粒直径。(3)相关性分析中,黄酮含量与清蛋白含量呈显著正相关;醇溶蛋白含量与主茎节数、基部20 cm内节数、主茎粗呈显著或极显著正相关,与千粒重、籽粒面积、周长、宽、直径呈显著或极显著负相关;淀粉含量与籽粒面积、长、直径呈显著正关系。(4)聚类分析将26个金苦荞品系分为了3个类群,其中类群I属于高淀粉、矮秆、多分枝、低果... 相似文献
8.
9.
用于筛选直链淀粉含量为中等的籼稻品种的分子标记 总被引:41,自引:0,他引:41
用PCR AccⅠ分子标记检测方法 ,检测了来自不同地区的 6 3个栽培水稻品种 (系 )蜡质基因第 1内含子剪接供体 1位碱基是G或是T。另外 ,还测定了这些水稻成熟种子的直链淀粉含量。结果显示该位置是G碱基的水稻品系成熟种子中直链淀粉含量均高于 2 0 % ,该位置是T的均低于 18%。在杂交育种过程中 ,这一分子标记可用于预测水稻植株种子的直链淀粉含量。对高直链淀粉含量的水稻亲本与中等直链淀粉含量的水稻亲本之间 5个籼型杂交组合F2 群体的分析表明 ,蜡质基因第 1内含子 1位碱基是G或是T与水稻种子中直链淀粉含量的高或低是紧密连锁 ,共同分离的。这些结果表明PCR AccⅠ分子标记检测方法可用于选育中等直链淀粉含量的籼稻新品系 相似文献
10.
转基因水稻胚乳中表达铁结合蛋白提高稻米铁含量 总被引:27,自引:0,他引:27
为提高我国稻米的铁含量,通过农杆菌介导将自行克隆的菜豆(Phaseolus limensis)铁结合蛋白(Ferritin)基因导入了一个高产粳稻(Oryaz sativa L.ssp.japonuica)品种中,获得17个独立的转基因水稻株系。分子检测证明,外源基因在多数转基因水稻植株基因组中有1~3个整合位点,并可稳定遗传。在水稻种子贮存蛋白谷蛋白基因GluB-1启动子的控制下,铁结合蛋白基因可在转基因水稻的种子中高效特异地表达,不同转化子中的表达量有明显不同。在转基因水稻种子中表达铁结合蛋白后对提高精米中的铁含量有明显的效果,相对于未转化对照最多可提高64%,而锌的含量并无明显变化。 相似文献
11.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
12.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
13.
14.
The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
15.
16.
Meng Miao Gang Deng Xiaobei Xiong Yang Qiu Wenda Huang Meng Yuan Fei Yu Shimei Bai Xi Zhou Xiaolu Zhao 《中国病毒学》2022,37(2):314-317
Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions. 相似文献
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions. 相似文献
17.
Dong Liu Xin Wang Yisong Wang Peigang Wang Dongying Fan Sichang Chen Yuguang Guan Tianfu Li Jing An Guoming Luan 《中国病毒学》2018,33(5):402-409
Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8+ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8+ T cells are involved in the pathogenesis of RE. 相似文献
18.
19.
Shen Jia-Yuan Li Man Xie Lyu Mao Jia-Rong Zhou Hong-Ning Wang Pei-Gang Jiang Jin-Yong An Jing 《中国病毒学》2021,36(1):145-148
正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016). 相似文献
20.
Renfei Lu Xiuming Wu Zhenzhou Wan Yingxue Li Lulu Zuo Jianru Qin Xia Jin Chiyu Zhang 《中国病毒学》2020,35(3):344-347
In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans. 相似文献