HPLC-ELSD法测定家桑野桑植株中1-脱氧野尻霉素的含量

采用HPLC-ELSD法对桑植株各部位所含的1-脱氧野尻霉素(DNJ)进行了含量测定,使用TSKgel Am-ide-80(4.6 mm×250 mm,5μm)色谱柱,流动相为:乙腈-水(84∶16,6.5 mM醋酸铵),流速为1.00 mL/min,柱温:30℃,线性范围为0.505μg～20.2μg(r=0.9991,n=6),平均回收率为94.95%(n=5),RSD=1.98。测定结果表明,野桑叶、嫩枝和根皮部位的DNJ含量较高,而家桑的叶、枝皮和根皮中含量较高。本方法准确度高、重现性好,能快速检测桑树资源及类似植物和相关产品中1-脱氧野尻霉素的含量。     

白刺花中3种生物碱的含量及HPLC指纹图谱

为建立白刺花高效液相指纹图谱,并测定白刺花不同部位(根、茎、叶)苦参碱、槐果碱和氧化苦参碱的含量。实验采用AgilentTC-C₁₈(2)色谱柱(250×4.6 mm,5μm),以乙腈-0.09%三乙胺水溶液(4∶96,磷酸调pH3.5)为流动相,流速为1.0 mL/min,紫外检测波长205 nm,柱温35℃。建立了白刺花的高效液相指纹图谱,确定了14个共有峰,样品的相似度在0.9以上。在该色谱条件下,各成分有较好的分离度,苦参碱、槐果碱和氧化苦参碱分别在6.34～101.5、6.47～103.5、8.38～100.5μg/mL范围内呈良好的线性关系(r>0.999)。用该方法测得白刺花根中苦参碱、槐果碱、氧化苦参碱的平均含量分别为8.204 8、1.237 1、66.147 6 mg/g;茎中分别为4.246 3、1.549 6、25.035 1 mg/g;叶中分别为9.314 8、6.947 4、1.573 4 mg/g。该方法简单、准确,可用于白刺花的质量控制。     

RP-HPLC法同时测定十种五加属植物叶中三萜Chiisanoside和Chiisaogenin

建立反相高效液相色谱法(RP-HPLC)测定chiisanoside和chiisanogenin的定量分析方法,利用最优色谱条件对十种中韩五加属植物叶中的三萜chiisanoside和chiisanogenin进行定量分析.研究结果表明,最佳色谱条件为:ODS-C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水(55:45);检测波长:205 nm;柱温:40℃;流速:1.0 mL/min.Chiisanoside和chiisanogenin线性范围和回归方程分别为16.5～ 181.5 μg/mL,Y=5498356X-9738(r=0.9995)和4.88～78.0 μg/mL,Y=5753131X-8289 (r=0.9979),加样回收率分别为98.87％(标准偏差为1.14％)和98.83％(标准偏差为0.72％).     

HPLC法测定不同产地鱼腥草中不同部位芦丁的含量

建立测定鱼腥草中芦丁含量的高效液相色谱分析方法。使用Eclipse XDB-C18色谱柱(4.6×150 mm,5μm),流动相为(A:0.1%磷酸水溶液,B:甲醇),柱温为20℃,流速为1.0 mL/min,检测波长为358 nm。测得芦丁的线性范围为0.02 mg/mL～0.50 mg/mL(r=0.9999),加标回收率为92.0%(n=3)。不同产地中芦丁含量测定结果表明,贵州产区的含量最高,河南的最低。不同部位中芦丁含量测定结果表明,叶中的含量均高于茎中5～10倍。本方法操作快速、简便、灵敏度高、重现性好,可使用于中草药中芦丁含量的测定。     

野生阿魏与栽培阿魏植物不同部位的挥发油及阿魏酸含量测定

目的:为了提高新疆阿魏资源储量分布和种植面积,保护野生资源,对阿魏药材的人工栽培品与野生品进行质量对比分析,为人工栽培环境采收的阿魏药材的质量评价提供参考依据。方法:采用挥发油测定法以及高效液相色谱法,对新疆不同产地野生阿魏和栽培品阿魏的挥发油和阿魏酸进行了含量测定。结果:人工压榨阿魏树脂,测得的挥发油含量中,各地野生品均符合药典标准(挥发油含量≥10%),栽培品中,阜康阿魏和伊宁产新疆阿魏达到药典标准。阿魏酸含量测定结果中,阿勒泰栽培品,阜康栽培品和伊宁栽培品的根和叶的阿魏酸含量,均大于野生品阿魏酸含量。结论:人工栽培环境采收的阿魏药材前景可观,不同部位均可以作为提取物的制备原料。     

HPLC法测定3种老鹳草药材中没食子酸和柯里拉京的含量

目的：建立老鹳草、野老鹳草、牻牛儿苗中没食子酸、柯里拉京含量测定方法.方法：采用HPLC法,色谱条件：色谱柱为Diamonsil C18 （2）色谱柱（150 mm×4.6 mm,5μm）;流动相为乙腈-0.1％磷酸梯度洗脱;流速为1.0 mL/min;检测波长为274 nm;柱温为25℃.结果：没食子酸和柯里拉京的线性范围分别是：0.2024μg～2.024 0 μg（r =0.999 6）、0.126 0 ～2.520 0μg（r =0.999 2）,平均回收率分别是97.4％（RSD=1.4％）、96.7％（RSD=0.9％）.结论：本法简便、准确、重复性好,可用于老鹳草药材的质量评价.     

蒙药材刺柏叶中槲皮苷的含量测定

本实验建立了蒙药材刺柏叶中槲皮苷的HPLC含量测定方法.色谱条件为:Diamonsil C18(4.6 mm ×250 mm,5μm)和Shim-Pack C18(4.6 mm× 250 mm,5μm),流动相:甲醇-0.01 mol/L磷酸二氢钾溶液-冰醋酸(40∶ 60∶ 1.5),检测波长:254 nm,流速:1.0 mL/min,柱温:30℃.槲皮苷进样量在40.2 ng ～603.0 ng范围内线性关系良好(r =0.9998),其平均加样回收率为100.45％,RSD为0.69％(n=6).本方法简便易行,结果准确,重复性好,可用于刺柏叶中槲皮苷的含量测定.     

HPLC-ELSD法测定闹羊花中Rhodojaponin-Ⅲ和Rhodojaponin-Ⅵ含量

为了建立中药闹羊花中Rhodojaponin-Ⅲ (Rj-Ⅲ)和Rhodojaponin-Ⅵ (Rj-Ⅵ)的含量测定方法,本文采用高效液相色谱-蒸发光散射检测方法(HPLC-ELSD)直接测定闹羊花75％丙酮水提取物中Rj-Ⅲ和Rj-Ⅵ 的含量.采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水(40∶60)为流动相,流速1mL/min,柱温30℃,ELSD(Varian 380-L)检测器,雾化器温度55℃,漂移管温度80℃,气体压力为25 psi,载气速度为1.6 L/s,增益为500.结果表明,闹羊花中Rj-Ⅲ和Rj-Ⅵ 的含量分别为1.21 mg/g和1.03 mg/g; Rj-Ⅲ和Rj-Ⅵ回归方程分别为y=2026.8X-789.2,R2=0.9979;Y=3777.6X-1849.5,R2=0.9980;分别 在0.4～2.83 μg/μL和0.6～4.2 μg/μL范围内,进样量与峰面积呈良好的线性关系,该方法简单可行,快速,精密度、重现性和稳定性均较好,可应用于中药闹羊花中Rj-Ⅲ和Rj-Ⅵ的含量测定.     

新疆戈宝麻花、种子及叶片部位芦丁含量比较

目的:比较新疆戈宝麻花、种子、叶片中芦丁的含量。方法:采用回流提取法提取芦丁,高效液相层析法法测定新疆戈宝麻不同部位中芦丁的含量。色谱柱为Eclipse-C18柱(4.6 mm×150 mm, 5 um);流动相为乙腈-0.25%磷酸溶液(V/V,14.5∶85.5);流速为1.0 mL/min;柱温为30℃;检测波长为360 nm;进样量为20 uL。结果:芦丁含量在0.01～0.1 mg/mL范围内与峰面积呈良好的线性关系,相关系数为0.993 6;其中花、种子、叶片中芦丁得率分别为:0.094%、0.014%、0.6%。结论:新疆戈宝麻不同部位芦丁的含量分布为:叶﹥花﹥种子,上述结果可为增加戈宝麻新药源和新的药用部位开发利用提供有力依据。     

RP-HPLC测定杭白芷不同部位5种香豆素的含量

目的:通过高效液相色谱测定栽培杭白芷不同部位中5种香豆素的含量,为合理利用栽培杭白芷资源提供依据.方法:采用Phenomenex Gemini C18(250 mm×4.60 mm,5μm)色谱柱;流动相为甲醇-水(70:30),流速0.8 mL·min-1,柱温30℃;UV检测波长310 nm.结果:栽培杭白芷不同部位的5种香豆素的含量差异显著.结论:栽培杭白芷的根中5种香豆素的总含量最高.     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

Interconnection of parameters of regulation system of lipid peroxidation and of morphophysiological parameters of mouse liver

A complex analysis of seasonal fluctuations of the mean group parameters of the system of regulation of lipid peroxidation has been performed in liver of Balb/c mice. Association of lipid characteristics and morphophysiological parameters is studied in the Balb/c mouse liver. An inter-connection is revealed between the liver index and the amount of lysoforms of phospholipids, the scale and character of the interconnection differing essentially depending on proportion of phos-phatidylcholine in mouse liver phospholipids.     

Time of detection of recessive genes: effects of system of mating and number of examined individuals

Summary Anthers were cultured from two sets of seven lines of hexaploid wheat (Triticum aestivum L.) with different cytoplasms, the euplasmic nucleus donors, Siete Cerros 66 and Penjamo 62, as well as their six alloplasmic lines derived from wild relative species of the genera Triticum and Aegilops. Significant cytoplasmic and nuclear effects but no cytoplasmic-nuclear interaction were found for embryogenic anther response, with the best performance of Penjamo 62 in Ae. kotschyi cytoplasm. Plant regeneration was not affected significantly by the cytoplasmic background of the lines cultured. The possible genetic implications of the observed cytoplasmic and nuclear influences on the in vitro haploid induction of wheat are discussed.