| HPLC法测定3种老鹳草药材中没食子酸和柯里拉京的含量 |
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| 引用本文： | 许敏,陈颖,段素敏,刘丽芳.HPLC法测定3种老鹳草药材中没食子酸和柯里拉京的含量[J].中国野生植物资源,2013(6):39-41,44. |
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| 作者姓名： | 许敏 陈颖 段素敏 刘丽芳 |
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| 作者单位： | 中国药科大学天然药物活性组分与药效国家重点实验室,江苏南京210009 |
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| 基金项目： | 国家药典委员会2010版一部标准研究课题（YS-229,YS-230） |
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| 摘 要： | 目的：建立老鹳草、野老鹳草、牻牛儿苗中没食子酸、柯里拉京含量测定方法.方法：采用HPLC法,色谱条件：色谱柱为Diamonsil C18 （2）色谱柱（150 mm×4.6 mm,5μm）;流动相为乙腈-0.1％磷酸梯度洗脱;流速为1.0 mL/min;检测波长为274 nm;柱温为25℃.结果：没食子酸和柯里拉京的线性范围分别是：0.2024μg～2.024 0 μg（r =0.999 6）、0.126 0 ～2.520 0μg（r =0.999 2）,平均回收率分别是97.4％（RSD=1.4％）、96.7％（RSD=0.9％）.结论：本法简便、准确、重复性好,可用于老鹳草药材的质量评价.
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| 关 键 词： | HPLC 老鹳草 野老鹳草 牻牛儿苗 没食子酸 柯里拉京 |
Determination of Gallic Acid and Corilagin in 3 Species of Lao-Guan-Cao by HPLC |
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| Xu Min,Chen Ying,Duan Sumin,Liu Lifang.Determination of Gallic Acid and Corilagin in 3 Species of Lao-Guan-Cao by HPLC[J].Chinese Wild Plant Resources,2013(6):39-41,44. |
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| Authors: | Xu Min Chen Ying Duan Sumin Liu Lifang |
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| Institution: | (State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China) |
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| Abstract: | Aim： To establish a method for determining gallic acid and corilagin in Geranium wilfordii Maxim. , Geranium carolinianum L. and Erodium stephanianum Willd. Methods： An HPLC method was performed on a Diamonsil Cls column （150 mm ×4.6 mm, 5 m） with acetonitrile -0.1% H3PQ as mo- bile phase. The flow rate was 1.0 mL/min,the detection wavelength was set at 274 nm and the column temperature was 25 ℃. Results： The calibration curve of gallic acid and corilagin were linear in the range of 0.202 4 - 2. 024 0 g （ r = 0. 999 6 ） and 0.126 0 - 2. 520 0 μg （ r = 0. 999 2）, respectively. The average recovery of gallic acid and corilagin were 97.4% （ RSD = 1.4% ） and 96.7% （ RSD =0.9% ）, respectively. Conclusion： The method is simple, accurate and suitable for the quality control for this herb. |
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| Keywords: | HPLC Geranium wilfordii Maxim Geranium carolinianum L Erodium stephanianum Willd gallic acid corilagin |
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