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1.
snthr-1Bao稀毛小鼠是本实验室培育的呈单基因隐性遗传的突变系小鼠,突变基因已被初步定位于第9号染色体末端;为了精确定位并鉴定snthr-1Bao稀毛小鼠的突变基因,将(C57BL/6J×snthr-1Bao)F1代互交繁殖F2代小鼠4 400余只,其中稀毛小鼠1 100只,并在2个微卫星、35个可能的简单序列重复标记(simple sequence repeat,SSR)及3个酶切扩增多态性序列(c1eaved amplified polymorphic sequences,CAPS)标记中找到4个合适的基因组标记。利用这些标记及F2代稀毛小鼠将突变基因精确定位到第9号染色体距着丝粒117.763 kb及119.129 kb之间1.367 Mb的范围内,在其间的21个基因中确定Plcd1为稀毛突变的强力候选基因。通过对基因组的直接测序,发现snthr-1Bao稀毛小鼠基因组上有一个14 883 bp的缺失,这一缺失包含了Plcd1基因的4—15号外显子及Vill基因的10—19号外显子。推测极可能是Plcd1基因缺失导致snthr-1Bao小鼠出现稀毛表型。  相似文献   

2.
为了研究地标(landmarks)是否影响犬蝠(Cynopterus sphinx)的空间记忆,我们通过室内模拟试验研究犬蝠和地标在觅食过程中空间记忆形成的关系。实验组按照每天地标数分别为0、2、4、8、0的数目连续进行5天实验,对照组不设地标进行相同条件的实验。结果显示,两组犬蝠第一次取食所用的时间与实验天数之间极显著相关(Pearson Correlations: 实验组r=-0.593, P<0.01;对照组r=-0.581, P<0.01);实验组取食成功率与实验天数之间无明显相关性(Pearson Correlations: r=0.177, P>0.05);对照组取食成功率与实验天数之间显著相关(Pearson Correlations: r=0.445, P<0.05)。实验组与对照组犬蝠第一次取食的时间差异不显著(GLM: F0.05,1=4.703, P>0.05),两组间取食的成功率差异也不显著(GLM: F0.05,1=0.849, P>0.05)。这些结果说明了随着时间增加,犬蝠对取食地的空间记忆逐渐形成,放置地标在犬蝠对取食地空间记忆形成的过程中无显著影响。  相似文献   

3.
2008年4—6月对青海湖湖区主要水鸟的稳定性碳和氮同位素组成进行了测定,依据营养级间的同位素富集效应以及营养级计算模型(TL水鸟=3+ (δ15N水鸟-1.78-δ15N浮游动物)/3.8),分析并确定了主要水鸟在生态系统中的营养级层次。相对于水生动物而言,水鸟的δ15N具有明显的富集效应,而δ13C不显著。依据稳定性同位素的营养富集效应模型,通过普通鸬鹚(Phalacrocorax carbo)和青海湖裸鲤(Gymnocypris przewalskii)间δ15N的比对,确定了该系统中排泄尿酸的水鸟稳定性氮同位素的富集因子为1.78‰。利用δ15N建立了稳定同位素比值与营养层次的关系。浮游植物、浮游动物、青海钩虾(Gammarus suifunensis)、裸鲤和水鸟的营养层次分别为1.64—2.06、2.35—2.83、2.63、3.64—4.20和3.35—4.93。由于湖区普通鸬鹚、渔鸥(Larus ichthyaetus)、棕头鸥(Larus brunnicephalus)和凤头(Podiceps cristatus)的食物来源主要是湖体中的裸鲤,由模型计算得到它们处在最高的营养级阶层;赤麻鸭(Tadorna ferruginea)、普通燕鸥(Sterna hirundo)和红脚鹬(Tringa totanus)属杂食性水鸟,营养阶层居中;而斑头雁(Anser indicus)由于主要以水生和陆生植物为食,处在最低的营养阶层。裸鲤资源未来的状况必将影响到普通鸬鹚、渔鸥、棕头鸥,以及凤头的生长、繁衍以及整个系统的群落结构和稳定性。  相似文献   

4.
 亚热带杉木(Cunninghamia lanceolata)和马尾松(Pinus massoniana)在我国森林资源中占有十分重要的地位, 研究它们的土壤与表层凋落物的呼吸有助于了解它们的碳源汇时空分布格局及碳循环过程的关键驱动因子。采用Li-Cor 6400-09连接到Li-6400便携式CO2/H2O分析系统测定湖南两种针叶林群落(2007年1月至12月)的土壤呼吸及其相关根生物量和土壤水热因子。研究结果表明: 杉木和马尾松群落中土壤呼吸的季节变化显著, 在季节动态上的趋势相似, 都呈不规则曲线格局, 全年土壤呼吸速率平均值分别为186.9 mg CO2&#8226;m–2&#8226;h–1和242.4 mg CO2&#8226;m–2&#8226;h–1。从1月开始, 两种群落的土壤呼吸速率由最小值33.9 mg CO2&#8226;m–2&#8226;h–1和38.6 mg CO2&#8226;m–2&#8226;h–1随着气温的升高而升高, 杉木群落到7月底达到全年中最大值326.3 mg CO2&#8226;m–2&#8226;h–1, 而马尾松群落到8月中旬达到最大值467.3 mg CO2&#8226;m–2&#8226;h–1, 土壤呼吸的季节变化与土壤温度呈显著的指数相关, 土壤温度可以分别解释土壤呼吸变化的91.7%和78.0%, 和土壤含水量呈二次方程关系, 土壤含水量可以解释土壤呼吸变化的5.4%和8.4%。由土壤呼吸与土壤温度拟合的指数方程计算Q10值, 杉木和马尾松群落中全年土壤呼吸的Q10值分别为2.26和2.13, Q10值随着温度升高逐渐减小。两种群落土壤呼吸的差异主要受群落植被的根生物量、群落的凋落物量的影响。  相似文献   

5.
回声定位蝙蝠耳蜗毛细胞静纤毛的长度特征   总被引:1,自引:0,他引:1  
描电子显微镜下观察爪哇大足鼠耳蝠(Myotis adversus)、白腹管鼻蝠(Murina leucogaster)、山蝠(Nyctalus plancyi)和马铁菊头蝠(Rhinolophus ferrumequinum)耳蜗毛细胞静纤毛, 测量其长度, 并与五种非回声定位哺乳动物耳蜗静纤毛长度进行比较. 研究发现: 回声定位蝙蝠耳蜗外毛细胞静纤毛较非回声定位哺乳动物相应位置的短; 回声定位蝙蝠耳蜗内毛细胞静纤毛长于其外毛细胞静纤毛, 而非回声定位哺乳动物内毛细胞静纤毛长度并无此规律. 我们认为, 回声定位蝙蝠耳蜗听毛细胞静纤毛长度的特点可能是对高频声波和回声定位适应的结果.  相似文献   

6.
2004年12月10日,在广西南宁地区马山县金伦洞捕到2只雄性印度假吸血蝠(Megaderma lyra: Megadermatidae, Chiroptera),分析其中的胃容物,发现有蝙蝠的残遗物,包括牙齿、后足、骨骼、毛发(棕黄色);未发现昆虫残遗物。通过对残遗物中牙齿(上颌齿式:2.1.3.3)的鉴定,与蝙蝠科鼠耳蝠属(Myotis)的齿式一致,因此确定印度假吸血蝠捕食了鼠耳蝠属的蝙蝠。  相似文献   

7.
千烟洲红壤丘陵区人工针叶林土壤CH4排放通量   总被引:3,自引:0,他引:3       下载免费PDF全文
 CH4在温室效应中起着重要作用,为估算中亚热带CH4的源汇现状,评价森林生态系统对温室效应的影响,采用静态箱-气相色谱法研究了千烟洲红壤丘陵区人工针叶林的土壤CH4 排放通量特征及水热因子对其的影响。对2004年9月~2005年12月期间的观测结果分析表明 :千烟洲人工针叶林土壤总体表现为大气CH4的吸收汇,原状林地土壤(Forest soil)情况下,CH4通量的变化为7.67~-67.17μg&;#8226;m-2&;#8226;h-1,平均为-15.53μg&;#8226;m-2&;#8226;h-1;无凋落物处理(Litter-free)情况下,CH4通量的变化是9.31~-90.36 μg&;#8226;m-2&;#8226;h-1,平均为-16.53μg&;#8226;m-2&;#8226;h-1。 二者对土壤CH4的吸收表现出明显的季节变化规律,秋>夏>冬>春,但无凋落物处理CH4变化幅度较原状林地土壤大,无凋落物处理吸收高峰出现在10月,最低值出现在翌年3月,原状林地土壤则分别在9月和翌年2月,均提前1个月。对土壤CH4吸收通量与温度和湿度的相关分析表明: 无论是原状林地土壤还是无凋落物处理情况下,土壤CH4通量都与地下5 cm的温度和湿度相关性最高。偏相关分析反映了不同季节水热配置对土壤吸收CH4通量的影响:冬季为12月~翌年2月,温度起主要作用;雨季3~6月,温度作用为主,随着温度的升高而升高,水分作用微弱;7~8月,CH4吸收通量随着湿度的降低而增加,但高温限制了CH4的吸收;秋季(9~11月)水热配置适宜,CH4通量达到高峰值。总之,CH4吸收通量随着温度的升高和 湿度的降低而增大,但温度过高会抑制其吸收。  相似文献   

8.
西双版纳热带季节雨林优势树种树干呼吸特征   总被引:3,自引:0,他引:3       下载免费PDF全文
 采用红外气体分析法(IRGA)原位监测了西双版纳热带季节雨林11种优势树种树干呼吸速率、1 cm深树干温度以及林内空气变化情况。研究发 现,11种优势树种的树干呼吸具有相同的季节规律,并且雨季均大于干季时的树干呼吸。树种间树干呼吸速率差异显著,在0. 823~2.727 μmol&;#8226;m-2&;#8226;s-1。树干1.3 m处所测南北方向树干呼吸无显著性差异。树干呼吸与树干温度显著相关(0.552<0.92),呈良好的自然指数回归关 系,Q10值为1.90~3.03。20 ℃时各树种的RT(总树干呼吸)速率为0.771~2.570μmol&;#8226;m-2&;#8226;s-1。  相似文献   

9.
2017-2020年期间,每年1月份对河南省济源市邵原镇布袋沟水库人工引水渠隧道内蝙蝠进行冬眠生态学特征调查,共发现2科5属7种蝙蝠在此冬眠,包括马铁菊头蝠(Rhinolophus ferrumequinum)、小菊头蝠(R.pusillus)、华南水鼠耳蝠(Myotis laniger)、白腹管鼻蝠(Murina leucogaster)、金管鼻蝠(Mu.aurata)、奥氏长耳蝠(Plecotus ognevi)和亚洲宽耳蝠(Barbastella leucomelas)。马铁菊头蝠是优势种(约52%-73%的冬眠个体),其次是小菊头蝠(约19%-37%)、华南水鼠耳蝠(约5%-8%),其余蝙蝠物种数量不足3%。2017-2020年冬眠蝙蝠个体总数呈增长趋势,但仍少于早期报道的数量。有42个隧道每年均有蝙蝠冬眠,而且不同年度冬眠数量也不尽相同。通过多元线性回归分析发现,隧道长度可能是影响蝙蝠冬眠栖息场所选择的主要影响因子(Adjusted R2=0.208,P=0.001)。每个隧道内,蝙蝠具有不同的冬眠栖点位置,约4/5的蝙蝠选择温暖且环境相对稳定的隧道深处(> 30 m)作为冬眠栖点,超过95%的个体选择长度> 60 m的隧道冬眠。蝙蝠具有不同的冬眠方式,绝大多数个体采用独栖方式进行冬眠(> 90%),少数采用聚集方式。不同的冬眠栖点和冬眠方式可能有利于冬眠成本优化。此外,栖点温度与蝙蝠体温之间呈显著正相关(R2=0.98,P < 0.001),而且蝙蝠冬眠期间的栖点温度具有种内和种间差异。研究结果为我国蝙蝠种群保护和冬眠场所管理提供科学依据。  相似文献   

10.
基于地形因子的藏狐生境评价及其空间容纳量的估算   总被引:3,自引:0,他引:3  
依据藏狐的“出现点”数据,基于藏狐对家域内地形因子的利用,作者对青海省都兰县沟里乡野生藏狐的生境质量进行初步评价,并在基于个体藏狐最小空间需求的基础上,估算了研究地区的空间环境容纳量。 选择海拔、坡度、坡位和地形起伏度等4个主要的地形因子,应用Bonferroni置信区间比较藏狐对各因子的利用及其可获得性来建立单个因子评价准则,并依据各因子的分布特征进行综合生境评价。研究结果:藏狐最适宜的海拔高度为4 050~4 300 m,坡度为5~20°,坡位为上坡位和下坡位;藏狐对地形起伏度因子没有明显的选择性。根据藏狐对不同生境因子的利用,及不同藏狐个体生境利用的一致性,建立的综合评价准则为:X i=(X2海拔×X2坡位×X坡度×X地形起伏度1/6。研究地区内适宜性区域面积约为17.4 km2,占总研究地区面积的38.8%,其中最适宜生境仅0.4 km2。藏狐家域面积从2.53 ~4.99 km2不等,不同个体间家域重叠指数(OI)从0.16~0.66不等。藏狐的最小空间需求为2.09~3.55 km2,研究地区藏狐的空间容纳量为7~12只。根据生境资源现状及监测结果表明,研究地区的藏狐种群数量保持稳定。  相似文献   

11.
The goal of this article was to establish reference ranges of the concentration of trace elements in human serum and to compare these results with those reported by other authors. We describe the sample preparation and measurement conditions that allow the rapid, precise, and accurate determination of Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum samples (n=110) by inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy and precision were determined by analyzing three reconstituted reference serum samples by comparison with other methods and by the standard addition procedure. The advantages of the ICP-MS method include short time of analysis of the elements mentioned, low detection limit, high precision, and high accuracy. Disadventages include a high risk of contamination due to the presence of some of the elements of interest in the environment, the relatively delicate sample handling, and the high cost of the equipment.  相似文献   

12.
Summary Concentration of N, P, K, Ca, Mg and S in summer groundnut crop was higher than in kharif while Zn, Fe, Mn and Cu contents were higher in summer crop. Kernel's N, P and Zn; Leaflet's Ca and Mn; Stem's K and Fe; Root's S and Cu and Petiole's Mg contents were highest. Shell's N, P, K, Mg, S, Zn and Cu; Kernel's Ca, Fe and Mn contents were the least. N, P, K, S, Zn and Cu concentrations decreased linearly as the crop grew. Ca, Mg, Fe and Mn concentrations did not display any distinct pattern. Ca concentration was positively correlated with pod yield in both the seasons.  相似文献   

13.
This report attempts to formulate reference ranges of elemental concentrations for 15 trace elements in selected human tissues and body fluids. A set of samples consisting of whole blood, blood serum, urine, milk, liver, and hair were chosen and considered for 15 elements of biological significance: As, Cd, Co, Cr, Cu, F, Fe, I, Hg, Mn, Mo, Ni, Pb, Se, and Zn. The results represent wholly or partially data received from 40 countries of the global regions of Africa, Asia, Europe, North, South, and Central America, Australia, and New Zealand. This survey, even if qualitative, has been useful in demonstrating certain trends of trace-element scenarios around the world. It is of course recognized that both diet and environment exert a strong influence on the distribution pattern of several elements, such as As, Cd, Mn, Pb, Se, and Zn. A limited comparison of the available information on soil status of different countries reflected some interesting associations for elements, such as Mn and Zn. Importantly, this study revealed that only a few countries were in a position to identify a reasonable amount of data on samples requested for this project. Regretably, for a number of countries, any dependable data for even such essential elements as Cu, Fe, and Zn were not available. In view of the nutritional importance of many elements, the time is ripe for concerted efforts by intergovernmental agencies to initiate investigations or commission task forces/projects to generate reliable reference data for selected global regions, which sadly lack data of any kind at present.  相似文献   

14.
15.
The variation with age of the 18 trace element mass fractions and some histological characteristics of intact prostate glands of 50 subjects aged 0–30 years was investigated by instrumental neutron activation analysis, inductively coupled plasma atomic emission spectrometry, and a quantitative morphometric analysis. Mean values ± standard error of the mean (M ± SΕΜ) for the mass fractions (in milligrams per kilogram wet tissue) of these trace elements in pre-puberty were: Al 28.5 ± 9.0, B 0.40 ± 0.11, Ba 1.48 ± 0.44, Br 10.5 ± 1.5, Ca 241 ± 30, Cl 3,203 ± 278, Cu 3.51 ± 0.89, Fe 33.7 ± 4.1, K 2,364 ± 145, Li 0.020 ± 0.004, Mg 153 ± 23, Mn 0.46 ± 0.06, Na 2,286 ± 130, P 1,391 ± 100, S 1,698 ± 132, Si 62 ± 11, Sr 0.38 ± 0.08, and Zn 27.6 ± 2.3. During puberty and postpuberty, when there is a significant increase in circulating androgens, the mean values were: Al 7.2 ± 1.4, B 0.21 ± 0.05, Ba 0.25 ± 0.06, Br 5.8 ± 1.0, Ca 433 ± 81, Cl 2,314 ± 201, Cu 1.77 ± 0.13, Fe 20.9 ± 1.6, K 2,585 ± 118, Li 0.0088 ± 0.0014, Mg 232 ± 27, Mn 0.34 ± 0.04, Na 1,875 ± 107, P 1,403 ± 98, S 1,673 ± 73, Si 22.2 ± 3.1, Sr 0.22 ± 0.03, and Zn 93.3 ± 8.9. Mean values (M ± SΕΜ) of percent volumes (%) of the stroma, epithelium and lumen in the prostate before puberty were 73.4 ± 2.6, 20.4 ± 1.7, and 4.45 ± 0.94, respectively, versus 46.5 ± 2.5, 38.5 ± 1.9, and 14.9 ± 1.2 during puberty and postpuberty. This work’s results confirm that the Zn mass fraction in prostate tissue is an androgen-dependent parameter. For the first time it has been demonstrated that the glandular lumen is a main pool of Ca, Mg, and Zn accumulation and that the stroma is a main pool of Al, B, Ba, Br, Cl, Cu, Fe, Mn, Na, and Si accumulation in the normal human prostate, for the age range 0–30 years. It was concluded that the Ca, Mg, and Zn binds tightly within the prostatic fluid, because the volume of glandular lumen reflects the volume of prostatic fluid.  相似文献   

16.
The effect of age and gender on major, minor, and trace element contents in the intact rib bone of 80 relatively healthy 15–55-year-old women and men was investigated. Contents or upper limit of contents of 16 chemical elements in the rib bone were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Mean values (M?±?SΕΜ) for the mass fraction of Ba, Ca, Cu, Fe, K, Li, Mg, Na, P, S, Sr, and Zn (milligram per kilogram of dry bone) were as follows: 2.54?±?0.16, 171,400?±?4,050, 1.35?±?0.22, 140?±?11, 1,874?±?71, 0.049?±?0.011, 2,139?±?38, 5,378?±?88, 75,140?±?1,660, 1,881?±?51, 291?±?20, and 92.8?±?1.5, respectively. The upper limits of contents of Al, B, Mn, and V were <7.20, <0.65, <0.36, and <0.03, respectively. Statistically significant tendency for the Ca, Mg, and P content to decrease with age was found in the human rib bone, regardless of gender. The mass fraction of Fe in the male rib bone increases with age. It was shown that higher Ca, Mg, Na, P, and Sr mass fractions as well as lower Fe content were typical of female ribs as compared to those in male ribs.  相似文献   

17.
The unitary conductances and permeability sequences of the rat connexin40 (rCx40) gap junction channels to seven monovalent cations and anions were studied in rCx40-transfected neuroblastoma 2A (N2A) cell pairs using the dual whole cell recording technique. Chloride salt cation substitutions (115 mM principal salt) resulted in the following junctional maximal single channel current-voltage relationship slope conductances (γj in pS): CsCl (153), RbCl (148), KCl (142), NaCl (115), LiCl (86), TMACl (71), TEACl (63). Reversible block of the rCx40 channel was observed with TBA. Potassium anion salt γj are: Kglutamate (160), Kacetate (160), Kaspartate (158), KNO3 (157), KF (148), KCl (142), and KBr (132). Ion selectivity was verified by measuring reversal potentials for current in rCx40 gap junction channels with asymmetric salt solutions in the two electrodes and using the Goldman-Hodgkin-Katz equation to calculate relative permeabilities. The permeabilities relative to Li+ are: Cs+ (1.38), Rb+ (1.32), K+ (1.31), Na+ (1.16), TMA+ (0.53), TEA+ (0.45), TBA+ (0.03), Cl (0.19), glutamate (0.04), and NO3− (0.14), assuming that the monovalent anions permeate the channel by forming ion pairs with permeant monovalent cations within the pore thereby causing proportionate decreases in the channel conductance. This hypothesis can account for why the predicted increasing conductances with increasing ion mobilities in an essentially aqueous channel were not observed for anions in the rCx40 channel. The rCx40 effective channel radius is estimated to be 6.6 Å from a theoretical fit of the relationship of relative permeability and cation radius.  相似文献   

18.
A new polynitro cage compound 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptcyclo [5.5.1.1(3,11).1(5,9)] pentadecane (NNNAHP) was designed in the present work. Its molecular structure was optimized at the B3LYP/6-31 G(d,p) level of density functional theory (DFT) and crystal structure was predicted using the Compass and Dreiding force fields and refined by DFT GGA-RPBE method. The obtained crystal structure of NNNAHP belongs to the P-1 space group and the lattice parameters are a = 9.99 ?, b = 10.78 ?, c = 9.99 ?, α = 90.01°, β = 120.01°, γ = 90.00°, and Z = 2, respectively. Based on the optimized crystal structure, the band gap, density of state, thermodynamic properties, infrared spectrum, strain energy, detonation characteristics, and thermal stability were predicted. Calculation results show that NNNAHP has detonation properties close to those of CL-20 and is a high energy density compound with moderate stability.  相似文献   

19.
《Ibis》1894,36(3):447-460
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20.
We investigated the uptake of inorganic elements (Be, Na, Mg, K, Ca, Sc, Mn, Co, Zn, Se, Rb, Sr, Y, Zr, Ce, Pm, Gd, and Hf) and the effect of Ca on their uptake in carrots (Daucus carota cv. U.S. harumakigosun) by the radioactive multitracer technique. The experimental results suggested that Na, Mg, K, and Rb competed for the functional groups outside the cells in roots with Ca but not for the transporter-binding sites on the plasma membrrane of the root cortex cells. In contrast, Y, Ce, Pm, and Gd competed with Ca for the transporters on the plasma membrane. The selectivity, which was defined as the value obtained by dividing the concentration ratio of an elemental pair, K/Na, Rb/Na, Be/Sr, and Mg/Sr, in the presence of 0.2 and 2 ppm Ca by that of the corresponding elemental pair in the absence of Ca in the solution was estimated. The selectivity of K and Rb in roots was increased in the presence of Ca. The selectivity of Be in roots was not affected, whereas the selectivity of Mg was increased by Ca. These observations suggest that the presence of Ca in the uptake solution enhances the selectivity in the uptake of metabolically important elements against unwanted elements.  相似文献   

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