哈慈健脑降压梳的治疗与保健功能

在药物疗法风靡世界的今天,人们对化学药品的毒副作用和药源性疾病的日益增多越来越感到不安,自然就把目光投向没有毒副作用,没有污染的自然疗法。人与自然是统一的,人起源于自然,依靠于自然、发展于自然、回归于自然。所以我国最早的医籍《黄帝内经》中提出了“天人合一”的观点。人类为了生存,在与大自然搏斗中发展和创造了种种利用自然来治疾病的方法,逐步形成了许多自然疗法,如体育疗法、气功疗法、按摩疗法、针灸疗法、推拿疗法、拔罐疗法、磁场疗法、刮痧疗法、森林疗法、泥土疗法、泉水疗法、梳头疗法等等,这些疗法各具特色…     

老年抑郁症患者自杀意念与生活事件、家庭功能及多导睡眠图参数的关系研究

摘要 目的：研究老年抑郁症患者自杀意念与生活事件、家庭功能及多导睡眠图参数的关系并予以分析。方法：选取从2019年1月～2020年10月期间我院收治的154例老年抑郁症患者纳入研究。将其按照是否存在自杀意念分作自杀意念组83例和无自杀意念组71例。比较分析两组生活事件、家庭功能及多导睡眠图参数等方面的差异,并以Spearman相关性分析明确老年抑郁症患者自杀意念与生活事件、家庭功能及多导睡眠图参数的关系。结果：自杀意念组正性事件、负性事件评分均高于无自杀意念组（均P＜0.05）。自杀意念组各项家庭功能评分均高于无自杀意念组（均P＜0.05）。自杀意念组N3期非快速眼动睡眠时间以及N3期非快速眼动睡眠占比均低于无自杀意念组（均P＜0.05）。经Spearman相关性分析可得,老年抑郁症患者自杀意念与各项生活事件评分、家庭功能评分均呈正相关,而与N3期非快速眼动睡眠时间以及N3期非快速眼动睡眠占比呈负相关（均P＜0.05）。结论：老年抑郁症自杀意念与生活事件、家庭功能及多导睡眠图参数密切相关,临床可通过对这些方面的观测来评估老年抑郁症的病情,以预防和减少老年抑郁症患者自杀行为的发生。     

离子导入疗法对防治糖尿病视网膜病变及改善症状的临床研究

目的：探讨离子导入疗法对糖尿病视网膜病变患者进行护理的临床效果。方法：选取88例（122眼）糖尿病视网膜病变患者,随机分为照组和实验组,其中对照组44例（58眼）,给予常规护理治疗;实验组44例（64眼）,在对照组基础上采用离子导入疗法进行护理。观察患者视网膜改变、视力的改善及自觉症状改善情况。结果：实验组实施干预后,视网膜改变、视力的改善、自觉症状的改善显效率明显高于对照组,差异有统计学意义（P〈0．01）。结论：离子导入疗法有助于改善糖尿病视网膜病变患者眼底血液循环。促进出血点吸收、改善临床症状,操作简单易行,患者易于接受。     

基因治疗研究现状与发展态势

基因治疗是指将人的正常基因或有治疗作用的基因通过一定方式导入人体靶细胞以纠正基因的缺陷或者发挥治疗作用,从而达到治疗疾病目的的生物医学技术。在临床应用中．基因治疗通常有体内疗法和体外疗法两种。体内疗法包括肌内注射、静脉注射、器官内灌输、皮下包埋等．其优点是将外源基因导入受体体内有关的器官组织和细胞内,简便易行,但其缺点是基因转染率较低。     

褪黑素受体与GABAA受体在褪黑素延长小鼠睡眠时间中的作用

目的：研究褪黑素受体和GABAA受体在褪黑素延长小鼠睡眠时间中的作用。方法：以翻正反射消失为睡眠开始的指标,至翻正反射恢复作为睡眠时间。观察不同受体激动剂或拮抗剂对褪黑素催眠作用的影响。结果：褪黑素3型受体拮抗剂盐酸哌唑嗪对褪黑素延长小鼠睡眠时间的作用无明显影响。GABA受体内源性激动剂GABA能明显增强褪黑素延长小鼠睡眠时间的作用,而GABAA受体上的印防己毒素结合位点的配基,即氯离子通道阻断剂印防己毒素能明显拮抗褪黑素的催眠作用,GABAA受体上的GABA结合位点的拮抗剂荷包牡丹碱则对褪黑素延长小鼠睡眠作用无明显影响。结论：褪黑素延长小鼠睡眠时间的作用与褪黑素3型受体无关,而与GABAA受体关系密切,其作用主要由印防己毒素结合位点介导。     

超声导入疗法治疗慢性乙型肝炎肝纤维化临床研究

目的：探讨超声导入疗法对乙型肝炎肝纤维化患者进行治疗的临床效果。方法：选择符合诊断标准的慢性乙型肝炎肝纤维化患者52例,随机分为试验组和对照组,各26例。对照组患者给予基本保肝治疗,试验组在对照组的基础上加用黄芪注射液进行超声导入,3个月为1个疗程。观察两组患者治疗前后症状、体征、血清肝纤维化指标、肝功能变化及影像学指标。结果：两组患者症状、体征均有不同程度的改善,差异无统计学意义（P〉0．05）;试验组血清肝纤维化指标明显改善,与对照组比较,差异有统计学意义（P〈0．05）;肝功能及影像学指标的改善更明显（P〈0．01）。结论：超声导入疗法对慢性乙型肝炎肝纤维化具有改善肝功能,减少肝细胞外基质的增生与沉积的效用,能够减轻或延缓肝纤维化的进展。     

重度抑郁症患者血清CXCL1、CCL11水平与生活事件和自杀意念的关系研究

摘要 目的：探讨重度抑郁症（MDD）患者血清CXC趋化因子配体1（CXCL1）、CC趋化因子11（CCL11）水平与生活事件和自杀意念的关系。方法：选取2020年1月-2021年6月我院收治的MDD患者100例作为病例组,选取同期100例健康体检的志愿者作为对照组,比较两组研究对象的血清CXCL1、CCL11水平、生活事件量表（LES）评定结果,采用Pearson法分析LES评定结果与CXCL1、CCL11水平的相关性。100例MDD患者根据有无自杀意念,分为自杀意念组（n=45）和无自杀意念组（n=55）,采用单因素及多因素Logistic回归分析MDD患者自杀意念相关影响因素。结果：病例组患者血清CXCL1、CCL11水平,负性事件刺激量、生活事件应激总分高于对照组（P＜0.05）,正性事件刺激量低于对照组（P＜0.05）;血清CXCL1、CCL11水平与负性事件刺激量呈正相关（P＜0.05）;本研究调查结果显示,MDD患者调查期间自杀意念发生率为45.00%,自杀意念组患者性别、自杀未遂史、病程、合并焦虑症状、精神质、神经质、内外向、掩饰性、CXCL1、CCL11、负性事件刺激量、生活事件应激总分与无自杀意念组比较差异有统计学意义（P＜0.05）,多因素Logistic回归分析结果显示,性别、精神质、神经质,血清CXCL1、CCL11水平,负性事件刺激量是MDD患者自杀意念发生的影响因素。结论：MDD患者血清CXCL1、CCL11水平升高,血清CXCL1、CCL11水平与负性事件刺激量存在一定的相关性,MDD患者出现自杀意念的概率较高,且血清CXCL1、CCL11水平及负性事件刺激量等均是其自杀意念发生的影响因素,临床工作中应针对相关影响因素积极采取应对措施。     

近红外气功信息治疗仪通过鉴定

由上海交通大学钱存泽讲师等研制的一种新型气功仿生仪——近红外气功信息治疗仪,在上海市卫生局和高教局的主持下,于六月十二日通过专家技术鉴定。参加鉴定会的专家教授一致认为,本仪器及其穴位辐照疗法用于治疗慢性活动性肝炎,国内外尚未见报道,值得进一步推广。五年前在上海亚明灯泡厂、上海交大附属工厂的积极合作下而试制的近红外气功信息治     

肉毒杆菌神经毒素作用机制的研究进展

肉毒杆菌神经毒素(BoNT)作用机制的研究近年取得的主要进展是：a.证明BoNT是通过降低神经递质释放系统对Ca²⁺的敏感性阻遏突触传递;直接将BoNT导入胞内不显示胆碱能专一性.b.BoNT与细胞表面的结合包括低亲和与高亲和相继两步,有不同的受体.c.BoNT的作用包括毒素与受体的结合,内吞和导入,变构、易位以及毒素作为酶在胞内酶裂与胞吐有关的蛋白质等过程.毒素重链的C端半段、N端半段及轻链分别是与上述过程有关的功能域.     

多导联在线阻抗检测的实现与分析

目的：提出了一种基于多导联生理信号采集装置平台的快速、稳定、低功耗的在线阻抗测量方法。方法：在线阻抗测量的实现主要有两个关键技术：正弦信号恒流源和数字带通滤波器,都主要是采用软件的方式实现的。结果：单极性和双极性两种模式四组测量阻值的变异系数均不超过10%,测量值的稳定性较高,可以通过多次测量求平均值的办法来修正测量误差,修正后的误差明显优于修正之前的误差。结论：由单极性和双极性两种模式实际阻抗测量值可知,测量值能够准确反映电极与人体表皮的接触情况,此种方法稳定可靠。     

Relative and combined effects of ethanol and protein deficiency on strontium and barium bone content and fecal and urinary excretion

To elucidate accumulation of minerals in human iliac arteries with aging, the content of minerals was analyzed by inductively coupled plasma atomic emission spectrometry. Bilateral common, internal, and external iliac arteries of 16 men and 8 women, ranging ages from 65 to 93 yr, were examined. It was found that an extremely high accumulation of calcium and phosphorus occurred in the common iliac artery at old age, being higher than that of the internal and external iliac arteries. It should be noted that the accumulation of calcium and phosphorus is the highest in the common iliac artery among the human arteries examined to date. Regarding sexual differences, the content of calcium and phosphorus in the common and internal iliac arteries was higher in women than in men, whereas their content in the external iliac artery was lower in women than in men.     

白蚁与动物及微生物的共生类型及其机制

综述了白蚁螱客的主要种类、共生关系及相关机制的研究进展。白蚁螱客中,已报道的动物种类达170种。在与动物的共生关系中存在偏利共生（宾主共栖和异种共栖）、互利共生和无关共生三种;在与微生物的共生关系中,存在与内生菌（原生动物、细菌、真菌和放线菌）和外生菌（蚁巢伞菌等）间的互利关系。指出了白蚁与螱客研究中存在的问题,给出了解决方案,并提出了今后可能的研究热点或方向,为白蚁的综合利用（如纤维素酶）及今后研究物种间的协同进化提供了基础资料。     

New amino-dithiaphospholanes and phosphoramidodithioites and their rhodium and iridium complexes

New sulfur derivatives of phosphoramidite ligands were synthesized and the impact of the sulfur unit on the spectroscopic properties of their rhodium and iridium complexes was investigated. The new ligands Bn₂NPSCH₂CH₂S^a(P-S^a) (Bn = benzyl, 4), Bn₂NPSCHCHS^a(CH₂)₃C^aH₂(P-S^a)(C^a-S^a) (6) and Bn₂NP(4-XC₆H₄OMe)₂ (X = S, 7a; X = O, 7b) were converted to the rhodium and iridium complexes trans-[Rh(CO)Cl(L)₂] (L = 4, 6, 7), [RhCl(COD)(L)] (L = 4, 6, 7), [IrCl(COD)(7a)] and [IrCl₂Cp∗(6)]. For comparison, some phosphoramidite complexes of these formulations also were synthesized. The new metal complexes were spectroscopically analyzed. For the carbonyl complexes, the ν_CO IR stretching frequencies were lower than for the corresponding phosphite and phosphoramidite ligands. The ¹J_PRh coupling constants for the rhodium complexes with the new ligands were also smaller than for the respective phosphoramidite and phosphite complexes. Finally, the ¹J_PSe coupling constants of the selenides of the new ligands were lower than those of the phosphoramidite ligands but higher than for PPh₃. The spectroscopic data reveal that the new thio ligands 4, 6 and 7a are more electron donating than phosphites and phosphoramidites but less electron donating than PPh₃.     

Astrocytes and Synaptosomes Transport and Metabolize Lactate and Acetate Differently

Astrocytes transport the monocarboxylate acetate, but synaptosomes do not. The reason for this is unknown, because both preparations express monocarboxylate transporters (MCT). The transport and metabolism of lactate, another monocarboxylate, was examined in these two preparations, and the results were compared to those for acetate. Lactate transport is more rapid in astrocytes than in synaptosomes, but of lower affinity (Kms of 17 and 4 mM, respectively). Lactate (0.2 mM) is metabolized to CO2 more rapidly in synaptosomes than in astrocytes (rates of 0.37 and 0.07 nmol x mg protein(-1) x min(-1), respectively). The reason for this is unclear, but cellular differences in lactate dehydrogenase isotype expression may be involved. Acetate is metabolized to CO2 more rapidly in astrocytes than in synaptosomes (rates of 0.43 and 0.02 nmol x mg protein(-1) x min(-1), respectively). This is likely due to cellular differences in the expression of monocarboxylate transporter subtypes.     

Rapporteur report: Basics and technology and metrology and standards

The first and second sessions of the Workshop focussed on the basics of ultrasound and infrasound, their applications in both industry and medicine, and metrology and protection standards for ultrasound applications.     

Prostaglandin and thromboxane production by human and guinea-pig macrophages and leucocytes

The ability of partially purified human and guinea-pig haematogenous cell populations, when cultured in vitro, to metabolise arachidonic acid (AA) has been studied. Supernatants from 24 hour cell culture have been subjected to analysis for products of AA metabolism by gas chromatography with electron-capture detection.The cell types studied were human peripheral blood monocytes (both glass adherent and non-adherent), neutrophils, eosinophils and leukemic leucocytes; thoracic duct lymphocytes and lung alveolar macrophages. From the guinea-pig, induced and non-induced macrophage or neutrophil enriched peritoneal exudate populations, lymph node cells, peritoneal eosinophils and peripheral blood platelets were examined. Supernatants were assayed for the presence of PGE₂, PGD₂, PGF_2α, TXB₂ and 6-keto-PGF_1α. In all types studied PGE₂ and TXB₂ were the major products formed. The identification of PGE₂ and TXB₂ was confirmed by GC/MS with multiple ion monitoring.The results have been compared with other reports and their possible significance discussed in relation to the proposed role of prostaglandins as mediators and modulators in immunopathology.     

人血管能抑素基因的克隆、表达及其表达产物的纯化和生物活性测定

以人胎盘脐带组织为材料,提取组织总RNA,用netRTPCR方法合成人血管能抑素cDNA基因,将该cDNA克隆进pSP72载体获得重组质粒pSP72C, DNA序列分析结果与预期序列一致。用BamHⅠ和NdeⅠ双酶切,切下pSP72C上的血管能抑素cDNA,插入pET3c载体的相应位点获得重组表达质粒pETC, 转化E. coli BL21(DE3), SDSPAGE分析显示：在IPTG诱导下,血管能抑素基因获得了高效表达,表达量约占菌体总蛋白的 27.9 %,主要以包涵体形式存在。包涵体经过洗涤、裂解、蛋白复性以及Sephadex G75凝胶过滤层析等步骤后,获得了纯度达91.4 %的人血管能抑素。CAM实验证明10 μg纯化蛋白就能显著抑制鸡胚新生血管生成。     

Cytoskeleton and mitochondrial morphology and function

It has been well established that the cytoskeleton is an essential modulator of cell morphology and motility, intracytoplasmic transport and mitosis, however cytoskeletal linkage to the organelles has not been unequivocally demonstrated. Indeed, cytoskeleton appears to be essential in determining and modulating gene phenotype as a function of cellular environment. According to recent studies, the organization of the cytoskeleton network together with associated protein(s) could be essential in regulating mitochondrial function and particularly the permeability of the mitochondrial outer membrane to ADP. The aim of this chapter is to summarize the main properties of the cytoskeletal environment of mitochondria and the possible role(s) of this network in mitochondrial function in myocytes.