水稻CPR5基因参与氧化胁迫响应

为探讨水稻(Oryza sativa L.)中CPR5 基因的功能,以其cDNA 文库为基础进行序列比对,并将1 个同源性较高的序列命名为OsCPR5.生物信息学分析表明,OsCPR5 的开放阅读框长度为1551 bp,编码516 个氨基酸.软件预测该编码蛋白可能是1 个具有5 个跨膜区的膜蛋白和核定位蛋白.组织表达和亚细胞定位分析表明,OsCPR5 在根中的表达水平较高,且广泛分布在细胞膜、细胞质和细胞核上.逆境胁迫分析实验表明,植物激素脱落酸(Abscisic acid, ABA)、过氧化氢(Hydrogen peroxide,H₂O₂)、氯化钠(NaCl)、甲基紫精(Methyl viologen, MV)等环境胁迫可诱导OsCPR5 的上调表达,其中氧化胁迫相关的MV 和H₂O₂ 处理效果最为明显.拟南芥(Arabidopsis thaliana)转基因株系atcpr5/OsCPR5 在浓度为0.5 μmol L^-1、 1.0 μmol L^-1 的MV以及6 mmol L^-1、 8 mmol L^-1 的H₂O₂ 处理下,种子萌发率明显高于atcpr5 突变体.这揭示了OsCPR5 基因在植物抗氧化胁迫响应中具有一定的作用.     

拟南芥AtCIPK23基因对烟草抗旱能力的影响

为了探索拟南芥AtCIPK23基因对烟草耐旱能力的影响,对3个转AtCIPK23基因阳性纯合株系KA13、KA14和KA44与野生型烟草K326（对照）进行了自然干旱处理,测定离体叶片的失水速率、叶绿素含量、相对电导率、脯氨酸和可溶性糖含量,并分析了转基因及野生型材料对活性氧的清除能力,对活性氧清除基因NtSOD、NtCAT和NtAPX及干旱胁迫相关基因NtDREB、NtLEA5和NtCDPK2的表达量进行检测。结果表明：（1）转基因烟草离体叶片的失水速率明显低于K326;自然干旱7 d后,野生型K326出现了明显的干旱胁迫症状;干旱7 d进行复水后,转基因株系的复水存活率明显高于K326。（2）转基因株系中的叶绿素、脯氨酸及可溶性糖含量比K326显著提高,电导率则明显降低。（3）野生型烟草K326中H₂O₂的积累量明显高于3个转基因株系,转基因株系中ROS清除机制的3个关键基因NtSOD、NtCAT和NtAPX被诱导上调表达。（4）抗旱相关基因NtDREB、NtLEA5和NtCDPK2仅在转基因烟草中受干旱诱导。研究认为,AtCIPK23基因可能具有提高植物抗旱能力的功能。     

白菜型油菜RbohC和RbohF基因克隆与表达分析

该研究以白菜型油菜（Brassica rapa L.）‘陇油6号’为实验材料,采用RT PCR方法克隆油菜RbohC和RbohF基因,并采用实时荧光定量PCR技术对RbohC、RbohF基因在不同组织及非生物胁迫下的表达进行分析,为深入研究油菜RbohC、RbohF基因的生物学功能提供依据。结果显示：(1) 成功克隆得到2个全长分别为3 050 bp和2 995 bp的油菜RbohC (GenBank登录号：XM_009134386) 和RbohF (GenBank登录号：XM_009114548) 基因序列。(2) 生物信息学分析显示,油菜RbohC和RbohF基因开放阅读框（ORF）分别为2 733 bp和2 847 bp,编码910和948个氨基酸,推测二者的蛋白质分子量分别为103 kDa和108 kDa,理论等电点分别为9.47和9.21; 油菜RbohC、RbohF编码的氨基酸序列与萝卜等多种植物相应蛋白氨基酸序列具有较高的同源性,且这些序列高度保守并含有NADPH氧化酶的典型保守结构域,包括2个可以与Ca²⁺结合的EF手性模体结构、6个跨膜结构域、黄素腺嘌呤二核苷酸结合结构域、NAD焦磷酸结合结构域和C末端区域中的NADP核糖保守结合位点。(3) 油菜RbohC和RbohF基因在根、茎、叶和下胚轴中均表达,无组织特异性,但RbohC基因在根中表达量最高, RbohF基因在下胚轴中表达量最高。(4) 低温、干旱、盐、ABA、H₂O₂处理都能够诱导油菜RbohC和RbohF基因的表达,但抗寒性强的 ‘陇油6号’的RbohC和RbohF基因对胁迫的响应更敏感,且RbohC基因的表达量均高于RbohF基因。(5) 用H₂O₂清除剂DMTU、NADPH氧化酶抑制剂DPI和IMD、MAPKK抑制剂U0126处理后,油菜RbohC和RbohF基因的表达均较对照下降,说明U0126和DMTU对油菜RbohC和RbohF基因的表达有抑制作用。研究认为,油菜RbohC和RbohF基因在油菜适应逆境胁迫中具有重要作用,两基因的表达均受MAPK激酶信号途径的调节,并受到H₂O₂的反馈调节,而且抗寒性强的‘陇油6号’品种中RbohC和RbohF基因对H2O2和MAPK激酶信号途径的响应更敏感。     

中国野生毛葡萄VqTLP15基因的克隆与抗病性功能研究（英文）

该研究以抗病品种中国野生毛葡萄‘商-24’和感病品种欧洲葡萄‘红地球’为材料,利用RT-PCR方法克隆TLP15基因,分别命名为VqTLP15和VvTLP15(GSVIVT01018769001),对其进行生物信息学分析、亚细胞定位、转化拟南芥,并接种不同病原菌观察分析转基因株系的抗性,采用qRT-PCR检测SA和JA/Eth信号途径以及调控气孔运动的相关基因表达。结果显示:(1)成功克隆获得VqTLP15基因的开放阅读框(ORF);氨基酸序列比对显示,VqTLP15基因与葡萄基因组网站欧洲葡萄‘黑比诺’VvTLP15和‘红地球’克隆的VvTLP15基因的同源性分别为98.99%和99.66%。(2)亚细胞定位表明,VqTLP15定位于细胞质。(3)成功获得VqTLP15转基因拟南芥株系(L1、 L2、 L3)。(4)接种观察发现:白粉菌处理7 d后转基因株系对白粉菌的抗性较野生型(Col-0)提高,且其叶片的白粉菌孢子浓度显著低于Col-0;灰霉菌诱导的叶片坏死性损伤在转基因株系(L1、L2和L3病斑面积40%的比例分别为71%、62%和67%)中显著大于Col-0(43%);接种PstDC3000后转基因株系叶片的病害表型没有Col-0明显,叶片孔径减小程度大于Col-0,且细菌浓度低于Col-0。(5)组织化学染色分析表明:白粉菌处理后转基因株系叶片胼胝质沉积、细胞死亡率和■水平都显著大于Col-0;灰霉菌处理后转基因株系的细胞死亡率、H_2O_2和■水平均高于Col-0;PstDC3000处理后细胞死亡率和■积累水平都高于Col-0。(6)qRT-PCR检测显示:接种白粉菌后,转基因株系中PR1和ICS1的表达水平均升高,PR1表达在接种72 h时达到峰值,而ICS1在接种120 h时达到峰值,LOX3的表达水平逐渐降低,并于接种120 h时降至最低水平,但仍高于Col-0;接种灰霉菌后,转基因株系中PR1、NPR1和PDF1.2基因的表达均上调,并在接种48 h时达到峰值,LOX3基因的表达水平下降,但仍高于Col-0;接种PstDC3000后,转基因株系中PR1、PDF1.2和NHL10的表达均高于Col-0,但WRKY53的表达低于Col-0, L1中COI1、FRK1、ATPPC2、FLS2、OST1的表达水平高于Col-0;接种flg22或LPS后, L1中COI1基因的表达低于Col-0,但ATPPC2、FLS2、OST1基因的表达水平高于Col-0。研究表明,过量表达VqTLP15基因降低了对白粉菌和PstDC3000的敏感性,增加了对灰霉菌的敏感性。VqTLP15基因可能通过介导水杨酸(SA)和茉莉酸/乙烯(JA/Eth)信号转导途径以及气孔免疫反应来参与植物的抗病防御反应,为葡萄抗病分子育种提供了一个可能的候选基因。     

转新型抗虫基因Vip3A棉花新种质的创制

该研究构建植物表达载体pBin438 Vip3A,通过农杆菌介导法转化棉花品种‘冀合713’,将新型抗虫基因Vip3A导入到棉花植株,创制对棉铃虫抗性的转基因棉花新种质。结果表明：(1)PCR检测Vip3A基因已经导入到棉花基因组中且能够稳定遗传。(2)室内抗虫性鉴定表明,与对照相比转基因植株对棉铃虫的抗性显著提高,并获得2株高抗和3株抗棉铃虫的转Vip3A基因株系。(3)Southern blotting结果显示,转基因株系BV01为单拷贝。(4)Elisa检测表明,外源Vip3A基因在BV01的根、茎、叶、花、种子中都有表达,在叶片中的Vip3A蛋白表达为苗期>蕾期>花期>铃期>吐絮期。该研究创制了新型抗虫转基因棉花材料,为培育棉花新型抗虫品种提供了种质资源。     

cry3A和vhb基因在转基因马铃薯中的表达

分别构建了含cry3A和cry3A+vhb基因的植物表达载体pBCry3A和pBC₃Vhb,并通过根癌农杆菌介导转化了马铃薯. 对转化再生植株进行PCR和DNA印迹分析表明,外源基因已整合到马铃薯基因组中, 且连续三代无性繁殖后转基因仍存在. ELISA分析表明cry3A基因在转基因植株中得到了高效表达, 在单转cry3A植株中最高表达量达0.1%, 转cry3A与vhb双基因株系中为0.065%. 水涝试验显示,转双基因且vhb mRNA的RT-PCR呈阳性的马铃薯植株,对低氧胁迫有较好的耐受性, 表明获得的上述转双基因马铃薯株系可能会具有很好的抗虫和耐涝性能.     

转OvDREB2B基因陆地棉鉴定及其对水分渗透胁迫的分析

通过花粉管通道技术,以该实验室自育陆地棉品系TH₁和TH₂为材料,将诸葛菜(Orychophragmus vidaceus)抗逆转录因子OvDREB2B基因构建到植物表达载体后,导入棉花基因组,经卡那霉素筛选和分子鉴定表明目的基因已整合到棉花基因组中并表达。将T₁代转基因植株和受体对照在温室中栽培,待植株生长至四叶一心时,用不同渗透势的PEG-6000水溶液进行渗透胁迫处理,分析探讨转基因植株的抗旱效果及其抗旱机理。结果显示:当渗透势为0和0.5 MPa处理时,转基因植株和对照无明显差异;当渗透势为0.8 MPa和1.1 MPa处理时转基因植株较对照抗旱性明显提高。当渗透势为1.1 MPa处理96 h时,对照植株F_v/F_m降至0.2左右,而转基因植株仍正常生长,F_v/F_m值约为0.51,而且初始荧光（F₀）值、净光合速率（P_n）、胞间CO₂浓度（C_i）、蒸腾速率（T_r）等一系列参数转基因植株都明显优于对照,表明DREB2B基因能够提高棉花对水分胁迫的耐受性。     

烟粉虱内共生菌groEL基因的原核表达条件研究

为了获得较多的G roEL蛋白,深入研究其性质,对烟粉虱内共生菌groEL基因表达的条件进行了研究。结果表明:诱导groEL基因原核表达的最佳IPTG浓度为100μmol/L;在35℃诱导培养,能够获得较高的蛋白表达量;最佳诱导培养时间为4~5h;最佳诱导培养的初始pH值为8.0;在振荡转速为120 r/m in、诱导培养时间为4~5h时,增大接种量,有利于原核基因的表达;添加少量的NH₄⁺有利于提高groEL基因原核表     

携带Paenibacillus polymyxa WLY78固氮基因簇(nif)的重组大肠杆菌Escherichia coli78-7转录组分析

[目的]来自Paenibacillus polymyxa WLY78的固氮基因簇（nifBHDKEfNXhesAnifV）可以转化入Escherichia coli中表达并使重组大肠杆菌合成有固氮活性的固氮酶。本文拟通过对重组大肠杆菌E.coli 78-7的转录组分析以提高其固氮能力。[方法]对固氮条件（无氧无NH₄⁺）和非固氮条件（空气和100 mmol/L NH₄⁺）培养的重组大肠杆菌E.coli 78-7进行转录组分析。[结果]nif基因在两种培养条件下显著表达,说明在重组大肠杆菌中可规避原菌中氧气和NH₄⁺对nif基因的负调控。对于固氮过程必需的非nif基因,如参与钼、硫、铁元素转运的mod、cys和feoAB,这些基因在两种培养条件下表达水平有差异。而参与铁硫簇合成的suf和isc基因簇在两条件下表达水平差异巨大。此外,参与氮代谢的基因在固氮条件下显著上调。[结论]重组大肠杆菌中与固氮相关的非nif基因在该菌的固氮过程中具有较大影响,本文对在异源宿主中调高固氮酶活性研究具有重要意义。     

香菜CsEF 1α基因克隆与表达分析

翻译延伸因子EF 1α(elongation factor 1 alpha)是细胞中最丰富的蛋白质之一,其在确保mRNA正确解码以产生细胞蛋白质方面发挥重要作用。该研究采用RT PCR扩增方法克隆香菜CsEF 1α基因序列,利用生物信息学对CsEF 1α基因结构、序列特征及系统进化等进行分析,并采用qPCR探究CsEF 1α基因在香菜不同生长时期和非生物胁迫下的表达模式,为进一步揭示EF 1α基因调控机制的研究奠定基础。结果显示：(1)成功克隆获得香菜CsEF 1α基因序列;CsEF 1α基因包含1个1 344 bp的开放阅读框,编码447个氨基酸,分子式为C₂₂₀₂H₃₅₄₄N₅₉₄O₆₄₄S₂₀,蛋白质分子量为49.29 kD,等电点为9.12;氨基酸序列组成中赖氨酸数量最多(49个,占11.0%),色氨酸数量最少(3个,占0.7%);属碱性蛋白。(2)CsEF 1α蛋白主要由无规则卷曲(36.91%)和α 螺旋(30.43%)构成,定位于细胞质;系统进化树分析显示,CsEF 1α与胡萝卜、野生番茄、青蒿素和非洲菊的亲缘关系较接近;启动子分析包括4种植物生长发育元件、3种激素响应元件和3种胁迫响应元件。(3)qRT PCR结果显示,CsEF 1α基因的表达量随着香菜生长发育时间的延长而升高,并且与转录丰度的变化一致;CsEF 1α基因对4种不同非生物胁迫的响应表达模式有所差异;随着胁迫时间的延长,在盐胁迫下CsEF 1α基因表现出先升高后降低趋势,而在低温、高温和干旱胁迫下表现出先降低再升高的趋势。研究表明,CsEF 1α基因参与了香菜对非生物胁迫的应答,在香菜生长发育和非生物胁迫中具有重要调控作用。     

Presence of Rhizobium Etli bv . Phaseoli and Rhizobium Gallicum bv . Gallicum in Egyptian Soils

Seven bean rhizobial strains EBRI 2, 3, 21, 24, 26, 27 and 29 identified as Rhizobium etli, and EBRI 32 identified as Rhizobium gallicum, isolated from Egyptian soils and which nodulated Phaseolus vulgaris efficiently, were subjected to hybridization with a nifH probe in order to estimate the copy number of this gene. Seven strains (EBRI 2, 3, 21, 24, 26, 27 and 29) which were only able to nodulate Phaseolus vulgaris, contained three copies of the nifH gene, consistent with their identification as Rhizobium etli bv. phaseoli. Only one strain (EBRI 32) which nodulated both Phaseolus vulgaris and Leucaena leucocephala, had one copy of nifH gene. This confirmed the classification of this strain as Rhizobium gallicum bv. gallicum.     

Effects of MULTIFOLIATE-PINNA, AFILA, TENDRIL-LESS and UNIFOLIATA genes on leafblade architecture in Pisum sativum

In order to dissect the genetic regulation of leafblade morphogenesis, 16 genotypes of pea, constructed by combining the wild-type and mutant alleles of MFP, AF, TL and UNI genes, were quantitatively phenotyped. The morphological features of the three domains of leafblades of four genotypes, unknown earlier, were described. All the genotypes were found to differ in leafblade morphology. It was evident that MFP and TL functions acted as repressor of pinna ramification, in the distal domain. These functions, with and without interaction with UNI, also repressed the ramification of proximal pinnae in the absence of AF function. The expression of MFP and TL required UNI function. AF function was found to control leafblade architecture multifariously. The earlier identified role of AF as a repressor of UNI in the proximal domain was confirmed. Negative control of AF on the UNI-dependent pinna ramification in the distal domain was revealed. It was found that AF establishes a boundary between proximal and distal domains and activates formation of leaflet pinnae in the proximal domain.     

Characterisation of cell wall polysaccharides, arabinogalactans-proteins (AGPs) and phenolics of Cola nitida, Cola acuminata and Garcinia kola seeds

Many Cola plant species are endemic to West and Central Africa. Cola acuminata and Cola nitida are used as masticatory when fresh, while the dried nuts are used for beverages and pharmaceutical purposes in Europe and North America. Garcinia kola seeds, that serve as a substitute for the true kola nuts, are used in African traditional medicine for the treatment of various diseases, including colic, headache and liver cirrhosis. Seeds extracts of G. kola are also known for their anti-inflammatory, antimicrobial and antiviral properties. To gain information on the chemical properties of the kolas, we have isolated and analyzed cell wall polysaccharides, arabinogalactan-proteins and phenolic substances from the seeds of the three kola species. The sugar composition of cell wall material of C. acuminata, C. nitida and G. kola revealed that Gal (up to 30%), Ara, GalA and Glc as the predominant monosaccharides, representing approximately 90% by mol of the total hydrolysable sugar present in this material. In Ammonium oxalate cell wall fraction, GalA was found to be the major sugar present in all kola species. In the alkali-soluble fraction, there were significant differences in the level of Glc and Gal. The level of Glc was high in C. acuminata and C. nitida while the level of Gal and Xyl were high in C. nitida and G. cola. Isolation and quantification of arabinogalactan-proteins demonstrate that G. kola seeds contained four to eight times more of these proteoglycans than the seeds of the other two species. Finally, analysis of soluble phenolic substances shows that caffeine and catechin were largely represented in C. acumina and C. nitida seeds, with caffeine accounting for 50% of all soluble phenolics. These findings indicate that the three Kola seeds are highly enriched in pectins and proteoglycans and that C. acuminata and C. nitida can be used as a possible source of caffeine and catechin.     

Genome Analysis inNeurospora crassa;Cloning of Four Lociarginine-1(arg-1),methionine-6(met-6),unknown-7(un-7), andribosome production-1(rip-1) and Associated Chromosome Walking

We have cloned fourNeurospora crassagenes by complementation analysis. Cloned genes include thearginine-1(arg-1),methionine-6(met-6),unknown-7(un-7), andribosome production-1(rip-1) loci. Chromosome walks were initiated in ordered cosmid libraries from the cloned loci. A total of about 700 kb of theNeurosporagenome is covered in these walks.     

The phylogenetic placement of Ostropales within Lecanoromycetes (Ascomycota) revisited

Results of molecular studies regarding the phylogenetic placement of the order Ostropales and related taxa within Lecanoromycetes were thus far inconclusive. Some analyses placed the order as sister to the rest of Lecanoromycetes, while others inferred a position nested within Lecanoromycetes. We assembled a data set of 101 species including sequences from nuLSU rDNA, mtSSU rDNA, and the nuclear protein-coding RPB1 for each species to examine the cause of incongruencies in previously published phylogenies. MP, minimum evolution, and Bayesian analyses were performed using the combined three-region data set and the single-gene data sets. The position of Ostropales nested in Lecanoromycetes is confirmed in all single-gene and concatenated analyses, and a placement as sister to the rest of Lecanoromycetes is significantly rejected using two independent methods of alternative topology testing. Acarosporales and related taxa (Acarosporaceae group) are basal in Lecanoromycetes. However, if the these basal taxa are excluded from the analyses, Ostropales appear to be sister to the rest of Lecanoromycetes, suggesting different ingroup rooting as the cause for deviating topologies in previously published phylogenies.     

Nomenclatural changes resulting from the transfer of tropical African Sarcostemma to Cynanchum ( Apocynaceae : Asclepiadoideae )

Summary  Four species of tropical African Sarcostemma are transferred to Cynanchum together with two subspecies of S. viminale. In addition, Sarcostemma mulanjense is reduced to subspecific rank under C. viminale.     

转Cry1Ac/1Ab基因棉花对异色瓢虫生长发育及其捕食功能的影响

【目的】为探究转Cry1Ac/1Ab基因棉花对异色瓢虫生长发育及其捕食功能的影响。【方法】以转Cry1Ac/1Ab基因棉与其亲本常规棉为实验材料,利用取食不同棉花品种叶片的棉铃虫饲喂异色瓢虫幼虫。【结果】与常规亲本棉相比,取食饲喂转基因棉花叶片的初孵棉铃虫幼虫的异色瓢虫幼虫从1龄发育至化蛹期时间延长0.77 d,但差异不显著;除1龄幼虫体重增加(0.0773 mg)外,其余各龄期幼虫体重均有所下降,但差异均不显著;异色瓢虫1、2、3、4龄幼虫对初孵棉铃虫捕食量均随棉铃虫密度的增加而增加,捕食功能反应均符合HollingⅡ圆盘方程。【结论】转Cry1Ac/1Ab基因棉花对异色瓢虫生长发育无显著影响,饲喂取食转Cry1Ac/1Ab基因棉花的棉铃虫对异色瓢虫捕食功能无显著差异。     

Prosthecium species with Stegonsporium anamorphs on Acer

Data from microscopic morphology, single-spore cultures, and DNA analyses of teleomorphs and anamorphs support the recognition of five species of Prosthecium with Stegonsporium anamorphs on Acer: P. acerinum sp. nov., the teleomorph of S. acerinum; P. acerophilum comb. nov., formerly known as Dictyoporthe acerophila; P. galeatum comb. nov., originally described as Massaria galeata; P. opalus sp. nov.; and P. pyriforme sp. nov., the teleomorph of S. pyriforme s. str. The morphology of both type specimens and freshly collected material was investigated. The teleomorphs have brown ellipsoidal ascospores with five distosepta and often a longitudinal distoseptum. The anamorphs of all species described here belong to Stegonsporium; their connection to the Prosthecium teleomorphs was demonstrated by morphology and DNA sequences of single spore cultures derived from both ascospores and conidia. The anamorphs and teleomorphs of all five Prosthecium species are described and illustrated by LM images, and a key to these species is provided. As perceived from this work, S. pyriforme is restricted to Europe and does not occur in North America, whereas S. acerinum is restricted to North America, not found in Europe. The host associations given in the literature are revised and evidence is provided that only A. opalus, A. pseudoplatanus, and A. saccharum are confirmed hosts of Prosthecium with Stegonsporium anamorphs. Molecular phylogenetic analyses of tef1, ITS rDNA, and partial nuLSU rDNA sequences confirm that the species with Stegonsporium anamorphs are closely related to P. ellipsosporum, the generic type species. Stilbospora macrosperma is confirmed as the anamorph of P. ellipsosporum by DNA data of single spore isolates obtained from both ascospores and conidia.     

中国黑粉菌属和利罗黑粉菌属

黑粉菌属是Roussel 1806年建立的,全世界记载有三百余种,主要寄生于禾本科,是经济作物及牧草的重要致病菌·长期以来,对黑粉菌的邢子使用过各种名称,如厚垣孢子,冬孢子及黑粉孢子等.本文采用黑粉孢子以区别锈菌的冬孢子. 芳’(1979)在《中国真菌总汇》中列出黑粉菌属五十种及一个变型.作者经过显微结构和超显微结构的研究,承认其中二十九种为正确名称,八种及一变型为异名,顶黑粉菌(Ustilago acrearus Berk.)由于错拼而被废弃.埃地黑粉菌(Ustilago emodensis Berk.)被转移至利罗粉菌属(Liroa).另有十一种黑粉菌因缺少标本留待今后订正.自1979年以后,杨信东(1983)增加黑粉菌属二种我国新纪录,K.范基和郭林(1986)描述一新种,四种新纪录.在本文中,作者描述一新种:鸢尾蒜黑粉(Ustilago ixiolirii Guo L) ,孢子堆生在蒴果内,不开裂,黑色,粉末状.黑粉孢子球形,近球形,稀椭圆形, 12.5-21×10-21μm,黑褐色,壁厚1-1.Sμm,纹饰脑状.是迄今生在石蒜科植物上唯一黑粉菌的种,其它几种黑粉菌均属条黑粉菌属.本文增加七种我国新纪录.共计四十九种,寄生于六科四十四属植物,主要是禾本科和蓼科.这仅是黑粉菌属研究的初步报告,在全国范围内大量采集黑粉菌标本后,作者相信会有更多新种和我国新纪录被发现.利罗黑粉菌属(Liroa)是从黑粉菌属(Ustaligo)分出的,此属为单种属.