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发现一株新的AINPV,与已报道的明显不同。其多角体呈三角形,直径1.0~1.24μm,单粒包埋型,每多角体内含52~78个大小为298~375×41~56um的病毒粒子。核衣壳大小为280~358×36~45um。该病毒株具较强毒力,以2×106~2×107PIB/ml的浓度感染3龄幼虫,6d死亡率达84.2~92%,LC50为1×104PIB/ml,浓度与死亡率的回归方程为y=3.28+0.43x。 相似文献
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基因工程干扰素(IFN—α2b)抗柯萨奇和单纯疱疹病毒效应 总被引:1,自引:0,他引:1
本文采用微量细胞病变抑制法在Wish 及Vero 细胞上研究了IFN—α2b 抗CoxB3 型及HSV—1 型和HSV—Ⅱ型病毒的作用。结果表明,安达芬和干扰能IHNα2b 在Wish 或Vero 细胞上500IU/ml 分别可以抗3log4 或1000TCID50 ,50IU/ml 可以抗2log4 或100 TCID50 ,5IU/ml 可以抗1log4 或10 TCID50 的CoxB3 型或HSV—1 型和HSV—Ⅱ型病毒感染细胞。提示安达芬和干扰能均显示明显的抗病毒效应,且二者无明显差别 相似文献
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猪瘟病毒在PK细胞和MPK细胞中繁殖过程的研究 总被引:15,自引:2,他引:13
以猪瘟病毒疫苗Thiverval株(T株)为实验材料,研究该病毒株在PK15细胞中增殖的基本特性与规律。在PK15细胞中,猪瘟病毒T株在感染后12h即可检测到子代病毒粒子。接毒后48h,几乎所有的细胞都被病毒感染;到60h,释放到培养液中有活性的病毒粒子达到最高峰,为107TCID50/mL。培养液中的病毒粒子在37℃半寿期只有3个小时。同时,建立了MPK细胞CSFVT株的感染模式,其CSFV的滴度可达108TCID50/mL。在此基础上,用抗CSFV包膜蛋白E2和非结构蛋白p120的单克隆抗体显示了病毒在细胞中增殖的部位,进而应用电镜技术观察到成熟的病毒粒子及可能处在不同发育阶段的子代病毒粒子 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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A.A. Zakeyah J. Whitt C. Duke D.F. Gilmore D.G. Meeker M.S. Smeltzer M.A. Alam 《Bioorganic & medicinal chemistry letters》2018,28(17):2914-2919
Microbial resistance to antibiotics is an unresolved global concern, which needs urgent and coordinated action. One of the guidelines of the Centers for Disease Control and Preventions (CDC) to combat antibiotic resistance is the development of new antibiotics to treat drug-resistant bacteria. In our effort to find new antibiotics, we report the synthesis and antimicrobial studies of 30 new pyrazole derivatives. These novel molecules have been synthesized by using readily available starting materials and benign reaction conditions. Some of these molecules have shown activity with MIC values as low as 0.78?µg/mL against four bacterial strains; Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Acinetobacter baumannii. Furthermore, active molecules are non-toxic to mammalian cell line. 相似文献
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The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
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Zsófia Czudor Mária Balogh Péter Bánhegyi Sándor Boros Nóra Breza Judit Dobos Márk Fábián Zoltán Horváth Eszter Illyés Péter Markó Anna Sipos Csaba Szántai-Kis Bálint Szokol László Őrfi 《Bioorganic & medicinal chemistry letters》2018,28(4):769-773
Cyclin-dependent kinases (CDKs) and Polo-like kinases (PLKs) play key role in the regulation of the cell cycle. The aim of our study was originally the further development of our recently discovered polo-like kinase 1 (PLK1) inhibitors. A series of new 2,4-disubstituted pyrimidine derivatives were synthesized around the original hit, but their PLK1 inhibitory activity was very poor. However the novel compounds showed nanomolar CDK9 inhibitory activity and very good antiproliferative effect on multiple myeloma cell lines (RPMI-8226). 相似文献