南蛇簕抗眼镜蛇毒的实验研究

目的探讨南蛇簕抗眼镜蛇毒的作用。方法抗眼镜蛇毒采用鲎试剂试验法。结果眼镜蛇毒与0.5EU/ml鲎试剂产生凝集反应的浓度在7.81μg/ml以上;南蛇簕提取液(含生药)为0.5g/ml浓度时可抗10倍量眼镜蛇毒的凝集反应。结论草药南蛇簕有较强的抗眼镜蛇毒作用。     

蛇伤药酒体外抗5种蛇毒的实验研究

目的探讨蛇伤药酒抗五步蛇、竹叶青蛇、眼镜蛇、蝰蛇、蝮蛇蛇毒的效果。方法采用鲎试剂试管凝集反应法。结果蛇伤药酒浓度为1.0 ml/ml时,对5种蛇毒均有破坏产生凝胶反应的作用;浓度为0.5ml/ml时,对五步蛇、竹叶青蛇、眼镜蛇蛇毒有破坏产生凝胶反应的作用;浓度为0.3 ml/ml时,仅对五步蛇毒有破坏产生凝胶反应的作用;浓度降至0.1 ml/ml时,对5种蛇毒均无破坏产生凝胶反应的作用。结论蛇伤药酒有较好的抗蛇毒作用,且对五步蛇毒作用最强。     

金枝蛇伤药酒体外抗蛇毒与抗菌的实验研究

目的探讨金枝蛇伤药酒抗5种蛇毒与4种细菌的效果。方法采用鲎试剂试管凝集反应法检测金枝蛇伤药酒对蝰蛇、竹叶青蛇、五步蛇、眼镜蛇、蝮蛇蛇毒的抗毒效果,采用滤纸片法检测金枝蛇伤药酒对大肠埃希氏菌、金黄色葡萄球菌、铜绿假单胞菌、粪肠球菌的抑制效果。结果金枝蛇伤药酒浓度为1.0ml/ml时,可抑制以上5种蛇毒;浓度为0.5ml/ml时,可抑制五步蛇、竹叶青蛇、眼镜蛇蛇毒;浓度为0.3ml/ml时,仅能抑制五步蛇蛇毒;浓度为0.1ml/ml时,无抑制作用。金枝蛇伤药酒对金黄色葡萄球菌、粪肠球菌抑制作用较强,对大肠埃希氏菌、铜绿假单胞菌也有一定的抑制作用。结论金枝蛇伤药酒有较好的抗蛇毒与抗菌的作用。     

大力王藤抗蛇毒及细菌内毒素的实验研究

目的探讨大力王藤药材抗蛇毒与细菌内毒素的作用。方法采用鲎试剂凝胶法测试大力王藤抗蛇毒及细菌内毒素的效果。结果大力王藤药液浓度为2 g/ml时,有抗眼镜蛇、竹叶青蛇、五步蛇毒和细菌内毒素的凝集作用;药液浓度为1.6 g/ml时,有抗蝰蛇毒的凝集作用;药液浓度为0.8 g/ml时,有抗蝮蛇毒的凝集作用。结论大力王藤有一定的抗蛇毒与细菌内毒素作用。     

几种蛇毒与鲎试剂产生凝胶反应的实验研究

目的探讨蛇毒能否与鲎试剂产生凝胶反应．建立中药及有效成分抗蛇毒作用的试验途径。方法采用鲎试剂试管凝胶反应法。结果不同的蛇毒能使鲎试剂产生凝胶反应的浓度不同,眼镜蛇毒与竹叶青蛇毒为5μg／ml．五步蛇毒0．32μg／ml．蝮蛇毒37．5μg／ml,蝰蛇毒2．5pg／ml。结论蛇毒能与鲎试剂产生凝胶反应。     

黑皮蛇、山白菜抗细菌内毒素的实验研究

目的探讨黑皮蛇、山白菜的抗细菌内毒素作用。方法采用鲎试剂凝胶法对黑皮蛇、山白菜抗细菌内毒素作用进行实验研究。结果在灵敏度为0.5EU/ml鲎试剂试验中,黑皮蛇、山白菜药液浓度1g/ml,均有抗细菌内毒素作用;不同药液浓度试验,抗细菌内毒素最低药液浓度:黑皮蛇为0.1g/ml,山白菜为0.05g/ml。结论黑皮蛇、山白菜均有较强的抗细菌内毒素作用。     

黑皮蛇、山白菜抗五种蛇毒的实验研究

目的研究黑皮蛇、山白菜抗五种蛇毒的药理作用。方法采用鲎试剂凝胶法对黑皮蛇、山白菜抗五种蛇毒的作用进行实验研究。结果黑皮蛇药液浓度在0.4g/ml以上、山白菜药液浓度在0.2g/ml以上,均能抗五种蛇毒与鲎试剂的凝集反应。结论黑皮蛇、山白菜有较强的抗眼镜蛇毒、竹叶青蛇毒、蝰蛇毒、蝮蛇毒和五步蛇毒作用。     

10种广东本地清热解毒中草药体外抗蛇毒作用的实验研究

目的 研究10种广东本地清热解毒中草药的抗蛇毒作用.方法 采用鲎试剂试管凝集反应法.结果 10种广东本地清热解毒中草药的药液浓度在0.5 g/ml以上时均有抗五步蛇、眼镜蛇、竹叶青蛇、蝰蛇、蝮蛇蛇毒的作用;降低药液浓度后,不同的药物对不同蛇毒抗毒作用有所不同.结论 广东土牛膝等10种本地清热解毒中草药对5种蛇毒有直接的...     

苦碟子注射液细菌内毒素检查法的探讨

目的：建立苦碟子注射液静脉点滴用药时细菌内毒素的检查方法。方法：采用2个厂家的鲎试剂对苦碟子注射液进行干扰试验。结果：鲎试剂浓度为0．25EU／mL时,苦碟子注射液稀释至1／30对细菌内毒素测定无干扰。结论：鲎试剂法检测苦碟子注射液静脉点滴用药时的细菌内毒素是可行的。     

中华眼镜蛇伤致局部组织损伤的临床研究

目的 探讨中华眼镜蛇咬伤致局部组织损伤的最佳治疗方法。方法 对蛇伤患者分别采用蛇伤药酒外敷、胰蛋白酶或糜蛋白酶局部封闭、抗眼镜蛇毒血清局部封闭共3种处理方法进行局部治疗,观察其疗效。结果 3种治疗方法从优到劣依次是：抗眼镜蛇毒血清局部封闭、胰蛋白酶或糜蛋白酶局部封闭、蛇伤药酒外敷。结论 中华眼镜蛇咬伤致局部组织损伤的治疗方法应首选抗眼镜蛇毒血清局部封闭,其次是胰蛋白酶或糜蛋白酶局部封闭,最差的是采用蛇伤药酒外敷。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.