太行山猕猴掌骨性别判别分析

为了了解成年太行山猕猴5根掌骨的性差大小。本文对太行山猕猴掌骨标本40例(雄:10, 雌:30)进行观察并选择掌骨的9项形态变量进行测量。数据用SPSS19.0软件进行多变量判别分析。结果表明: 5根掌骨长度等变量在性别之间有明显差异。用全模型法5根掌骨的性别正确判别率范围为94.1%~100.0%, 用逐步判别法掌骨性别正确判别率范围为93.8%~97.5%。有关掌骨的9个形态变量, 长度变量首先被挑选出来, 说明在性别判别中起重要作用。分别用左右侧掌骨的判别函数来判别性别时侧别差异不明显。结论: 用每根掌骨的形态变量建立判别函数可以有效地区分性别, 对非人灵长类掌骨标本的性别鉴定有一定的理论意义和应用价值。     

猕猴颊齿大小的性差研究初报

对28例太行山成年猕猴(♂10,♀18)的上、下颌颊齿齿冠面积进行测量。运用SPSS 10.0统计软件的多变量分析,选择有关颊齿变量建立性别判别函数。结果表明:猕猴颊齿具有明显的性差。选择不同的变量和选择不同的判别函数其性别正确判别率不同。上、下颌颊齿的性差有一定差异。使用逐步判别法建立判别函数,其性别正确判别率上颌颊齿为89.3%,下颌颊齿为92.3%。     

太行山猕猴髋骨变量研究初报

本文对22例（♂11,♀11）成年太行山地区猕猴髋骨有关变量进行测量,通过有关变量单因素方差分析、R型聚类和回归分析,结果表明：太行山猕猴的髋骨变量有较明显的自身特征,髋骨有关变量在雌雄之间有明显差异。     

太行山猕猴肩胛骨和肱骨性差的研究

以26例成年太行山猕猴肩胛骨和肱骨的15项线性变量为对象,运用SPSS 12.0统计分析软件对其进行双变量相关分析、异速生长分析和主成分分析,以探讨各变量之间的性差大小以及产生的原因.结果表明,太行山猕猴雌雄之间存在明显的性差.其原因可能与性成熟时间、生长速率的不同以及各自的生活方式和运动行为有关.     

太行山猕猴掌骨和蹠骨长度的性别判别

研究太行山猕猴掌骨和蹠骨长度的性别差异。对24例掌骨和26例蹠骨的长度变量进行测量, 用SPSS13.0统计软件进行单因素方差分析和多变量判别分析。结果表明: 掌骨和蹠骨的长度变量在性别之间有极显著性差异(P＜0.00)。各掌骨和各骨的长度顺序在侧别之间和性别之间有一定差异, 但是尚未达到统计学意义( P> 0.05) 。用全模型法和逐步判别法根据长度变量分别建立性别判别函数, 其性别正确判别率掌骨分别为91.7%和93.8%, 而蹠骨的性别判别率为84.6%。表明猕猴掌骨和蹠骨长度性别之间存在明显性差。使用掌骨和蹠骨长度变量进行性别鉴定有一定理论价值和应用价值。     

太行山猕猴掌骨和踽骨长度的性别判别

研究太行山猕猴掌骨和蹠骨长度的性别差异.对24例掌骨和26例蹠骨的长度变量进行测量,用SPSS13.0统计软件进行单因素方差分析和多变量判别分析.结果表明:掌骨和踱骨的长度变量在性别之间有极显著性差异(P<0.00).各掌骨和各踱骨的长度顺序在侧别之间和性别之间有一定差异,但是尚未达到统计学意义(P>0.05).用全模型法和逐步判别法根据长度变量分别建立性别判别函数,其性别正确判别率掌骨分别为91.7%和93.8%,而蹠骨的性别判别率为84.6%.表明猕猴掌骨和蹠骨长度性别之间存在明显性差.使用掌骨和踱骨长度变量进行性别鉴定有一定理论价值和应用价值.     

太行山猕猴掌面花纹嵴数不对称性研究

为了解太行山猕猴掌面花纹嵴数的方向性不对称和波动性不对称的特征和性差,对26例(雌18例,雄8例)肤纹完整清晰的太行山猕猴的掌面花纹嵴数进行计数,用SPSS 13.0统计软件进行T-检验和相关分析.结果表明:在方向性小对称有关变量中存在性别差异,变量之间存在弱的负相关,波动性不对称量不存在性差,变量之间的相关系数有不同程度的性差.     

太行山猕猴下颌骨变量相关性研究

本研究测量了１８例（雄４,雌１４）成年太行山猕猴下颌骨的１２项变量。通过对下颌骨变量的性别判别分析和相关分析。结果显示：太行山猕猴下颌骨变量有其自身特征,与其它猕猴下颌骨资料比较表现出一定差异;用Ｏｔｔｅｓｔａｔ方法对有关变量建立性别判别函数式,其判别率达８６．８６％,下颌骨变量相关性研究表明,各变量之间有正相关,也有负相关,大多数变量之间相互程度较差,不同变量之间其相关程度存在一定差异。     

太行山猕猴牙齿性别差异研究

以太行山猕猴牙齿为对象,主要研究各牙齿的性差大小及分布模式.测量43例太行山猕猴牙齿的2个主要变量.数据通过SPSS统计分析软件处理.性差大小用雄雌差值(Dif)和性差百分比(P.D)表示.结果表明,太行山猕猴牙齿性差主要表现在犬齿性差"区"和第3臼齿,主要是MD径,越靠近犬齿其性差越大,下颌的更为明显.表明太行山猕猴牙齿的不同变量、不同位置性差程度不同.本研究在进行性差推断和种间比较时有一定的实用价值和理论价值.     

太行山猕猴跗骨形态与两性分化

观察太行山猕猴跗骨形态结构,并初步探讨其两性分化的模式。根据猕猴齿式及骨垢愈合程度选择成年太行山猕猴跗骨标本52例(雄性15例,雌性37例)。选择4块跗骨(舟骨、骰骨和2块楔骨)的5个形态变量进行观察。数据分析采用SPSS 20.0统计软件,组间差异检验采用单因素方差分析。猕猴跗骨5个变量的两侧差异性检验无统计学意义(P>0.05)。跗骨性别之间差异有统计学意义(P<0.05),均为雄性大于雌性;第3楔骨的前关节间宽性差最大(F=85.719,P<0.001);其次是舟骨的前关节宽(F=54.213,P<0.001)和第1楔骨的前关节间长(F=51.865,P<0.001)。猕猴足骨与其他灵长类相比差异具有统计学意义。猕猴跗骨与人类跗骨形状和数量不同,存在着物种、生活习性和运动类型等差异,研究猕猴跗骨的形态结构和性差对生物进化和种间比较有重要意义。     

太行山猕猴牙齿与颅长的相关性

对太行山猕猴牙齿有关变量做了测量,统计,并就其与颅骨长间的异速生长和相关程度进行了研究。结果表明,太行山猕猴牙齿具有明显的性别差异,雄性牙齿的变异程度在犬齿和中央门齿表现尤为突出。在线性型或面积方面,雄性牙齿的异速生长均快于雌性和雄＋雌,此与其生活史对策相适应,在雄性,雌性或雄＋雌,下颌齿变量与颅骨长的相关程度比上颌更为密切,与有关资料的比较表明,太行山猕猴与金丝猴之间的差异小于与菲氏叶猴之间的差异。     

三种笼养灵长类活动时间分配的比较研究

采用焦点动物法和连续记录法对3种笼养灵长类活动时间分配进行比较研究。结果表明,川金丝猴Rhi-nopithecus roxellanae和熊猴Macaca assamensis用于休息的时间多于猕猴Macaca mulatta,移动时间则相反;川金丝猴和猕猴用于理毛的时间多于熊猴。不同性别年龄组之间的活动时间分配有差异,主要表现在川金丝猴母亲用于理毛的时间明显多于成年儿子,用于玩耍的时间则相反;猕猴成年雄性的移动时间和理毛时间多于成年雌性,玩耍时间则少于成年雌性;熊猴成年雌性的移动时间多于成年雄性,与成年个体相比,幼体花更多的时间用于玩耍,而相应的减少了其休息时间。     

A review of sexual initiating behavior by male and female cynomolgus monkeys and some species comparisons

The sexual initiating behavior of male and female cynomolgus monkeys (Macaca fascicularis) observed during standard laboratory tests is reviewed and compared with that of rhesus monkeys (M. mulatta) observed under identical conditions. Species differences in sexual behavior are related here to differences in habitat, sexual dimorphism, and the dominance gradient between the sexes. Compared with rhesus monkeys, cynomolgus monkeys appear to be more arboreal, less sexually dimorphic, and have a smaller dominance gradient between the sexes. They exhibit a facultative single-mount copulatory pattern rather than the serial mount pattern of the rhesus monkey. Female cynomolgus monkeys are less dominated than rhesus females by their male partners. Direct aggression between mates is more frequent and redirected aggression occurs less often than in rhesus monkeys. These behavioral differences affect the interpretation of changes in initiation rates that occur (1) during the menstrual cycle, (2) when females are ovariectomized and given hormone replacement treatments, and (3) when males are castrated and treated with androgens. We conclude that estradiol in the female and testosterone in the male increase the sexual motivation of both the treated and the untreated partner. Valid interpretations of changes in initiation rates depend on accurate and exclusive definitions of behavior and on a consideration of the behavioral context in which they are made.     

Paralysis due to a glomangioma in a Macaca mulatta

A glomangioma at the 6-7 thoracic intervertebral space caused compression of the spinal cord with posterior paralysis in an irradiated 20-year-old female rhesus monkey (Macaca mulatta). Glomangiomas are tumors of arterial-venous shunts, are rare and have only been reported in irradiated rhesus monkeys.     

太行山猕猴下颌的生物力学研究

采用定点方法,对太行山猕猴下颌骨有关变量作了测量、统计和分析。通过对太行山猕猴下颌生物力学、异速生长的研究表明,雄性下颌相对较长,与此有关的生物力学特点是下颌髁后缘至M1近中侧长、下颌髁后缘至M3下次小尖长和下颌支高等阻力臂减小,而下颌髁长、下颌髁宽、下颌体高Ⅱ、下颌体宽Ⅱ、下颌联合长等变量则增强,使咀嚼力增加。这些特点均与其生态对策密切相关。研究结果还与有关材料进行了比较,表明太行山猕猴与国内其他地区的猕猴存在着明显差异。     

猕猴、食蟹猴群中STLV-1病毒感染状况研究初报

[目的]摸清STLV-1感染现状,从而有效地降低STLV-1在猕猴、食蟹猴群中的的感染率。[方法]采用STLV-1ELISA法对猕猴、食蟹猴血清进行抗体检测。结果本中心送美国BioReliance公司的2455只出口猴血清,103份血清呈STLV-1抗体阳性,19份血清呈STLV-1抗体可疑,其余血清均为STLV-1抗体阴性。[结论]猕猴、食蟹猴群中STLV-1的平均感染率为4.97%,其中猕猴STLV-1感染率为2.7%,食蟹猴STLV-1感染率为5.4%,是猕猴STLV-1感染率的2倍;随着年龄的增长,猕猴(食蟹猴)STLV-1的感染率也随之升高。     

Ultrasonic assessment of pregnancy and fetal development in three species of macaque monkeys

Serial ultrasonic assessments of gestational sac (GS) and fetal biparietal diameter (BPD) were performed in the Japanese monkey (Macaca fuscata fuscata), rhesus monkey (Macaca mulatta), and crab-eating monkey (Macaca fascicularis). In the Japanese monkey, GS increased linearly for 3-8 weeks, whereas BPD increased in a linear-quadratic manner over 8 weeks to term. Ultrasonic assessments of spontaneously aborted fetuses with BPD growth retardation and diagnosis of a pelvic chocolate cyst also were reported.     

Maternal or fetal origin of rhesus monkey (Macaca mulatta) amniotic fluid leukocytes can be identified by polymerase chain reaction using the zinc finger Y gene.

Leukocytes can be found in substantial numbers within the intrauterine tissues and amniotic fluid of women, and play a central role in the pathophysiology of infection-related preterm labor by their production of proinflammatory mediators. It remains unclear whether these leukocytes represent a fetal immune response, a maternal response, or a combination of the two. The objective of this study was to develop a test in the rhesus monkey (Macaca mulatta) suitable for determining the percentage of male fetal cells present in a population of leukocytes recovered from blood or amniotic fluid. We found inadequate specificity for rhesus monkey cells using commercial human Y-chromosome paint kits (fluorescence in situ hybridization (FISH)). Human-specific primers for the repetitive Y chromosome DYZ-1 locus employed in the polymerase chain reaction (PCR) produced an unacceptable percentage of false positives. However, we successfully developed a PCR-based test using rhesus-specific primers for the zinc finger Y (ZFY) locus. Densitometry of PCR products from known ratios of male and female adult peripheral leukocytes generated a linear standard curve which provided quantitative results and required only 400 cells per sample. The rhesus beta globin (RBG) gene served as an internal control. The PCR test correctly discriminated the sex of peripheral leukocytes in 20 adult males, 20 adult females, two male fetuses, and one female fetus. Serial samples of amniotic fluid from four chronically catheterized rhesus monkeys bearing male fetuses were used to confirm the utility of this assay for quantifying fetal cells in amniotic fluid. In conclusion, we have developed a PCR test which is suitable for distinguishing male from female cells in adult and fetal blood and in amniotic fluid, which lends itself to a variety of diagnostic and biologic applications in the rhesus monkey and potentially in other nonhuman primates.     

Permeability of the rhesus monkey oocyte membrane to water and common cryoprotectants

Successful cryopreservation of oocytes of the rhesus monkey (Macaca mulatta) would facilitate the use of this valuable animal model in research on reproduction and development, while providing a stepping stone towards human oocyte cryopreservation and the conservation of endangered primate species. To enable rational design of cryopreservation techniques for rhesus monkey oocytes, we have determined their osmotic and permeability characteristics in the presence of dimethylsulfoxide (DMSO), ethylene glycol (EG), and propylene glycol (PROH), three widely used cryoprotectants. Using nonlinear regression to fit a membrane transport model to measurements of dynamic cell volume changes, we estimated the hydraulic conductivity (L(p)) and cryoprotectant permeability (P(s)) of mature and immature oocytes at 23.5 degrees C. Mature oocyte membranes were most permeable to PROH (P(s) = 0.56 +/- 0.05 microm/sec) and least permeable to DMSO (P(s) = 0.24 +/- 0.02 microm/sec); the permeability to EG was 0.34 +/- 0.07 microm/sec. In the absence of penetrating cryoprotectants, mature oocytes had L(p) = 0.55 +/- 0.05 microm/min/atm, whereas the hydraulic conductivity increased to 1.01 +/- 0.10, 0.61 +/- 0.07, or 0.86 +/- 0.06 microm/min/atm when mature oocytes were exposed to DMSO, EG, or PROH, respectively. The osmotically inactive volume (V(b)) in mature oocytes was 19.7 +/- 2.4% of the isotonic cell volume. The only statistically significant difference between mature and immature oocytes was a larger hydraulic conductivity in immature oocytes that were exposed to DMSO. The biophysical parameters measured in this study were used to demonstrate the design of cryoprotectant loading and dilution protocols by computer-aided optimization.     

Effect of altered hormonal states on transaminase enzymes in the genital ograns of immature female rhesus monkeys

The effect of ovariectomy and hormone replacement therapy on the activity of glutamate oxalacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) in the oviduct, uterus, cervix and vagina of immature female rhesus monkeys (Macaca mulatta ) was studied. In intact animals, GOT activity was high in the oviduct, whereas GPT was high in the vagina. Ovariectomy suppressed the activity of both the enzymes in varying degrees. Estradiol dipropionate stimulated GOT in the oviduct and uterus, whereas progesterone increased this enzyme in the uterus. Sequential treatment of two hormones inhibited the enzymes in all the tissues; GPT was, however, stimulated in the fundus region of the uterus. The study demonstrated the presence of transaminase enzymes in the genital tissues of rhesus monkey and their differential response to sex steroids.