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1.
在1/3海水培养基上筛选豆瓣菜耐盐变异体 总被引:7,自引:1,他引:6
系统地研究了豆瓣菜(NasturtiumofficainaleR.Br)茎段外植体对6-BA,NAA和2,4-D的反应,确定了MS培养基附加6-BA2.0mg/L,2,4-D0.2mg/L为豆瓣菜愈伤组织诱导,继代培养基;MS培养基附加6-BA4.0mg/L为芽再生培养基;MS基本培养基为植株的生根的扦插繁殖培养基,将325个豆瓣菜茎切段外植体接种到含1/3海水的愈伤组织诱导培养基上,17块外植体 相似文献
2.
百脉根愈伤组织原生质体再生植株 总被引:1,自引:0,他引:1
百脉根无菌苗幼茎在含2.0mg/L-,2,4-D,0.1mg/L2-ip的MS培养基上诱导和继代培养愈伤组织。选取绿色松散颗粒愈伤组织分离原生质体。原生质体培养在调整珠KM8P,V-KM,MS和SH培养基上「含300mg/L,CH,2%CW,2%蔗糖,6%葡萄糖,2.0mg/L,2,4-D,0.5mgg/L,BA,5mmol/L MES」,原生质体再生细胞均能分裂,并形成小愈伤组织,但以KM80为 相似文献
3.
4.
大叶紫花苜蓿愈伤组织原生质体再生植株 总被引:15,自引:0,他引:15
大叶紫花苜蓿下胚轴诱导的愈伤组织在继代培养基上生长快速,易于分散。继代第12d的愈伤组织原生质体的得率为6.5×107/g鲜重。原生质体培养基为SH基本培养基,含有1.0mg/L2,4-0、0.5mg/LBA、2.0g/LCH、2%蔗糖、6%葡萄糖、5mmol/LMES,培养密度为1.0×105/mL。培养至第12d时的原生质体再生细胞植板率为3.7%。由原生质体形成的小愈伤组织在含2.0mg/L2,4-D的MS固体培养基上大量增殖。增殖的愈伤组织转移至2.0mg/L2-ip+0.1mg/LNAA的B5培养基上,形成体细胞胚并发育成完整植株。 相似文献
5.
红景天叶片诱导再生植株 总被引:2,自引:0,他引:2
红景天片接种在MS培养基上,诱导形成愈伤组织,再稳定在MS附加6-BA2mg/1+IAA0.25mg/l的培养基上产生大量丛生芽,丛生芽培养于B5附加IAA0.5mg/l的培养基上可导生根形成完整植株。 相似文献
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7.
决明组织培养的研究 总被引:1,自引:0,他引:1
以草决明无菌苗子叶为外植体,接种于7类诱导愈伤组织的培养基上:A.MS+2.02,4-Dmg/L(以下单位省略)+0.3BA+0.2NAA.B.MS+0.22,4-D十0.2BA+2.0NAA.C.MS+1.02,4-D+0.5BA+0.2KT.D.MS+0.7BA+1.5NAA+0.1KT.E.MS+1.52,4-D+0.7BA十0.2MAA.F.MS+0.42.4-D+1.0NAA+0.1KT.G.MSB(MS的无机成份和B5有机成分)+0.15NAA+BA,KT和ZT各0.5。8~15天后分别有90~99.6%的子叶片被诱导出愈伤组织,并且F.与C.类培养基对诱导愈伤组织比较理想,放于G培养基上的愈伤组织有9.2~30.2%的芽分化率。芽在生根培养基1/2MS+0.2IBA中、98%生根,形成完整的再生植株。 相似文献
8.
栀子组织和细胞培养生产天然食用色素的研究:Ⅰ.愈务组织… 总被引:4,自引:0,他引:4
在诱导出愈伤组织的基础上,对各种不同的培养条件进行分析研究,考察它们对愈伤组织生长和栀子黄色素产生的影响,筛选出适宜的生长培养基:B5+TBA 1mg/1+kT0.23mg/、MG-5+IBA 1mg/+KT0.23mg/、B5+IAA1.5mg/l和生产培养基;M-9+IAA 1mg/l、M-9+IAA 1.5mg/l。并且,获得了几个色素含量较高的愈伤组织系。另外,还研究了含色素和不含色素的愈 相似文献
9.
小麦叶片愈伤组织及其再生植株的诱导 总被引:17,自引:0,他引:17
小麦幼苗基部外植体在补加2,4-D的MS、N6、BA1(3)培养基上均可诱导出愈伤组织,2,4-D的最适浓度为2.0mg/L;愈伤组织的增殖速度与切段部位及基本培养基有关,其中在以MS补加2.0mg/L2,4-D的培养基上诱导出的愈伤组织增长速度最快;最后讨论了小麦愈伤组织幼苗诱导率低的原因及可能解决的方法。 相似文献
10.
中麻黄悬浮培养体系的建立 总被引:5,自引:1,他引:4
本文用中麻黄无菌苗为外植体,其切段培养在附加2mg/L2,4-D和0.5mg/L 6 BA的MS培养基上,全部脱分化形成白色疏松愈伤组织。愈伤组织继代培养于MS+0.5mg/L2,4-D+0.2mg/L6BA+0.2mg/L NAA+4%蔗糖的培养某上。以继代培养愈伤组织为材料进行悬浮培养,培养基为附加0.2mg/L2,4-D+0.1mg/L6BA+0.1mg/LNAA+2%蔗糖的MS液体培养基,得到分散性好,细胞形状接近圆形,细胞大小均一,细胞团多由2-30个细胞组成的悬浮培养体系。第三代悬浮培养细胞增长率为0.35g·fw/20ml·d,细胞有丝分裂指数为11.2%。条件培养和高密度接种可缩短延迟期,条件培养不能提高分裂指数,1g/10ml接种密度可使分裂指数提高至21.2%。 相似文献
11.
卫星搭载亚麻后代中PEG和NaCl抗性系的初步筛选 总被引:8,自引:0,他引:8
把空间生物学和细胞工程相结合,通过组织培养技术对其离体筛选,得到抗1.2%NaCl和35%PEG的愈伤组织,将所得抗性系愈伤组织在2.0mg/L6-苄基氨基嘌呤、0.5mg/L吲哚乙酸的MS培养基上分化得到完整的植株。抗性系能在胁迫条件下保持高的生长速度和高效的脯氨酸合成能力,表明空间诱变与组织培养相结合有望可成为筛选抗胁迫变异系的有效途径。 相似文献
12.
卫星搭载亚麻后代中PEG和NaCl 总被引:3,自引:0,他引:3
把空间生物学和细胞工程相结合,通过组织培养技术对其离体筛选,得到抗1.2%
NaCl和35% PEG的愈伤组织。将所得抗性系愈伤组织在2.0 mg/L 6-苄基氨基嘌呤、0.5
mg/L吲哚乙酸的MS培养基上分化得到完整的植株。抗性系能在胁迫条件下保持高的生长速度和高效的脯氨酸合成能力。表明空间诱变与组织培养相结合有望可成为筛选抗胁迫变异系的有效途径。 相似文献
13.
Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 10 mg/l adenine sulphate (Ads) and 3% (w/v) sucrose incubated in the light or in the dark. Somatic embryos germinated (95–98%) into normal plants and were transferred to soil with 95% success. In vitro flowering was induced on shoots developed from nodal explants taken from somatic embryo regenerated plants of Bambusa vulgaris, Dendrocalamus giganteus and Dendrocalamus strictus on half-strength MS basal medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA), 0.5 mg/l Ads, 0.5 mg/l gibberellic acid (GA3) and 3% sucrose.Abbreviations BAP
6-benzylaminopurine
- Kn
kinetin
- Ads
adenine sulphate
- IBA
indole-3-butyric acid
- NAA
1-naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- MS
Murashige and Skoog (1962) basal medium
- GA3
gibberellic acid 相似文献
14.
Somatic embryos were obtained from callus cultures derived from leaf explants of the winged bean, Psophocarpus tetragonolobus (L.) DC. Initiation and development of the somatic embryos occurred with a two-step culture method. Callus cultures initiated on MS medium with NAA and BAP, upon transfer to a new medium with IAA and BAP, produced somatic embryos. Maximum embryogenesis of 60% was obtained on induction medium with 0.5 mg/l NAA plus 1.0 mg/l BAP followed by transfer to a secondary medium with 0.1 mg/l IAA and 2.0 mg/l BAP. Optimal embryo germination and plantlet development was achieved on MS medium with 0.2 mg/l BAP plus 0.1 mg/l IBA. The regenerated plants were successfully transferred to glasshouse conditions.Abbreviations MS
Murashige and Skoog (1962) medium
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphthaleneacetic acid
- IAA
Indole-3-acetic acid
- IBA
Indole-3-butyric acid
- BAP
6-benzylaminopurine
- KN
Kinetin 相似文献
15.
Plant regeneration in Kentucky bluegrass (Poa pratensis L.) via coleoptile tissue cultures 总被引:1,自引:0,他引:1
Summary Plant regeneration in Kentucky bluegrass (Poa pratensis L. cv. Touchdown) via culture of seedling tissues was investigated. When coleoptile, leaf, and stem sections of dark-germinated seedlings were cultured on Murashige and Skoog (MS) medium, different types of callus were produced, depending on the expiant source and growth regulator combinations. Only compact-friable callus (type 3) and moderately compact, friable callus (type 2) produced shoots upon subculture. The nonstructured watery callus (type 4) produced roots without shoots. Shoot differentiation from callus tissues was highest when the culture medium contained 0.2 mgL–1 picloram + 0.01 mgL–1 -naphthaleneacetic acid (NAA). Calli grown from coleoptiles had higher shoot regeneration frequency (32%) than that obtained from either stem sections (12%) or young leaf tissues (2%) of the same seedlings. Some organogenic callus lines produced exclusively green plants, while others produced albino shoots or a mixture of green and albino shoots. The green plants were multiplied in a medium containing 0.1 mgL–1 BAP plus either 0.2 mgL–1 picloram or 0.1 mgL–1 indole-3-acetic acid (IAA). Over 90% of the cultures in the shoot proliferation medium produced roots in 4 weeks. The rooted plants were successfully established in soil medium and grown in the greenhouse.Abbreviations BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic acid
- picloram
4-amino-3,5,6-trichloropicolinic acid
- TDZ
thidiazuron 相似文献
16.
The morphogenetic potential of node, internode and leaf explants of Brahmi [Bacopa monniera (L.) Wettst.] was investigated to develop reliable protocols for shoot regeneration and somatic embryogenesis. The explants
were excised from shoots raised from axillary buds of nodal explants cultured on Murashige and Skoog (MS) basal medium. Presence
of 6-benzylaminopurine (BA) or kinetin influenced the degree of callus formation, from which a large number of shoot buds
regenerated. Leaf explants gave the largest number of shoot buds followed by node and internode explants. BA was superior
to kinetin; BA at 1.5 – 2.0 mg/l appeared to be optimum for inducing the maximum number of shoot buds. MS + 0.1 mg/l BA +
0.2 mg/l indole-3-acetic acid was the most suitable for shoot elongation. Elongated shoots were rooted on full- or half-strength
MS medium with or without 0.5 – 1.0 mg/l indole-3-butyric acid or 0.5 – 1.0 mg/l α-naphthaleneacetic acid. The rooted plants were successfully established in soil. Calli derived from nodal explants cultured
on MS medium containing 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), when subcultured on MS medium containing 0.1 or 0.5
mg/l BA or 0.2 mg/l 2,4-D + 0.1 or 0.5 mg/l kinetin, developed somatic embryos. The somatic embryos germinated either on the
same media or on MS basal medium, and the resulting plantlets were successfully transplanted to soil.
Received: 25 September 1996 / Revision received: 23 October 1997 / Accepted: 12 November 1997 相似文献
17.
Efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar Centennial on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. Centennial expiants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.Abbreviations BAP
6-benzylaminopurine
- NAA
1-naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- MS
Murashige and Skoog (1962)
- GUS
-glucuronidase
- IAA
indole-3-acetic acid
- NPT II
neomycin phosphotransferase II 相似文献
18.
A protocol for in vitro multiplication of Capparis decidua (Forsk.) Edgew. has been developed from cultured leaves procured from multiplying axillary shoots on the cultured nodal explants.
The highest efficiency of shoot formation was observed on Murashige and Skoog (MS) medium containing 2 mg dm−3 benzyladenine (BA) and 0.5 mg dm−3 1-naphthaleneacetic acid. The regenerated shoots were transferred to MS medium containing 3 mg dm−3 BA for growth and proliferation. Shoots above 2 cm in length were transferred to MS medium supplemented with 1 mg dm-3 indole-3-butyric
acid plus 0.5 mg dm−3 indole-3-acetic acid for root induction. No variation was detected among the micropropagated plants by randomly amplified
polymorphic DNA (RAPD) markers. 相似文献
19.
荞麦高频离体再生及发根农杆菌转化体系的建立 总被引:7,自引:0,他引:7
荞麦无菌苗下胚轴切段在不同激素配比的MS培养基上诱导愈伤组织,出愈率均为100%。在2.0mg/L2,4-D和1.5mg/L 6-BA组合下诱导产生的愈伤组织;转入2.0mg/L 6-BA和1.0mg/L KT的MS培养基,再生苗分化率在80%以上。根尖色体分析表明再生植株具一定的遗传稳定性。发根农杆菌A4转化荞麦下胚轴和子叶获得发状根,纸电泳检测所有随机取样测定的发状根均有相应冠瘿碱的存在。 相似文献
20.
Summary Sodium chloride-tolerant plantlets of Dendrocalamus strictus were regenerated successfully from NaCl-tolerant embryogenic callus via somatic embryogenesis. The selection of embryogenic
callus tolerant to 100 mM NaCl was made by exposing the callus to increasing (0–200 mM) concentrations of NaCl in Murashige and Skoog medium having 3% (w/v) sucrose, 0.8% (w/v) agar, 3.0 mg l−1 (13.6 μM) 2,4-dichlorophenoxyacetic acid (2,4-D), and 0.5mg l−1 (2.3μM) kinetin (callus initiation medium). The tolerance of the selected embryogenic callus to 100 mM NaCl was stable through three successive transfers on NaCl-free callus initiation medium. The tolerant embryogenic callus
had high levels of Na+, sugar, free amino acids, and proline but a slight decline was recorded in K+ level. The stable 100 mM NaCl-tolerant embryogenic callus differentiated somatic embryos on maintenance medium [MS medium +3% sucrose +0.8% agar +2.0
mg l−1 (9.0 μM) 2,4-D+0.5 mg l−1 (2.3 μM) kinetin] supplemented with different (0–200 mM) concentrations of NaCl. About 39% of mature somatic embryos tolerant to 100 mM NaCl germinated and converted into plantlets in germination medium [half-strength MS+2% sucrose+0.02 mg l−1 (0.1 μM) α-naphthaleneacetic acid +0.1 mg l−1 (0.49 μM) indole-3-butyric acid] containing 100 mM NaCl. Of these plantlets about 31% established well on transplantation into a garden soil and sand (1:1) mixture containing
0.2% (w/w) NaCl. 相似文献