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宫粉郁金的组织培养和快速繁殖 总被引:8,自引:1,他引:7
1植物名称宫粉郁金(Curcuma kwangsiensis)。2材料类别 块茎萌动芽。3培养条件 萌动芽生长培养基:(1)MS+6-BA1mg·L-1(单位下同)+NAA0.2。不定芽增殖与愈伤组织诱导培养基:(2)MS+6-BA10+KT5;(3)MS+6.BA10;(4)MS+6-BA5+KT2.5;(5)MS+6-BA5;(6)MS+6-BA2+KT1。生根培养基:(7)MS+NAA0.5;(8)MS+6-BA0.5+NAA0.5;(9)MS。以上培养基均加0.7%琼脂,3%蔗糖,pH5… 相似文献
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蝴蝶兰根段的组织培养 总被引:38,自引:2,他引:36
1 植物名称 蝴蝶兰(PhalaenopsisMellerGold“NFS”)。2 材料类别 根段。3 培养条件 (1)愈伤组织的诱导及分化培养基:B5+NAA1.5mg·L-1(单位下同)+KT0.2+CM150ml·L-1+3%蔗糖;(2)原球茎增殖培养基:B5+GA0.05+CH120+3%蔗糖;(3)小苗生长培养基:1/2MS+20%香蕉泥+2%蔗糖;(4)诱导生根培养基:1/2MS+IBA0.3+2%蔗糖。上述培养基均加0.2%活性炭,0.58%琼脂粉,pH为5.5;培养基在121℃高… 相似文献
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紫菀花序芽培养及植株再生 总被引:1,自引:0,他引:1
1植物名称紫菀(Astertataricus)。2材料类别花序芽。3培养条件愈伤组织、不定芽诱导及增殖培养基:(1)MS+6-BA0.5~2.0mg·L-1(单位下同);生根培养基:(2)MS+NAA0.5~2.0+6-BA0.2,(3)1/2MS(大量元素减半)+NAA0.5~2.0+6-BA0.2。上述培养基均加0.8%琼脂和3%蔗糖,高温高压灭菌前pH值调至5.8。培养温度为(2312)℃,每天光照12h,光照度为1500~2000lx4生长与分化情况4.1愈伤组织及不定芽的诱导剪取长约0… 相似文献
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玫瑰海棠的组织培养及快速繁殖 总被引:16,自引:0,他引:16
1植物名称玫瑰海棠(Begonnia mannii)。2材料类别幼芽、叶片。3培养条件以MS为基本培养基。诱导丛生芽培养基:(1)MS+KT1.0mp·L-1(单位下同)+NAA0.1。丛生芽增殖培养基:(2)MS+KT1.0+NAA0.05;(3)MS+6-BA0.5+NAA0.05。诱导生根培养基:(4)1/2MS+NAA0.2。上述培养基均加3%蔗糖,0.8%琼脂,pH5.8。培养温度(26±2)℃,光照度约为2000lx,光照14h·d-1。4生长与分化情况4.1无菌材料的获得将玫瑰海棠… 相似文献
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沙葱的组织培养和植株再生 总被引:7,自引:0,他引:7
1植物名称沙葱(Alliummongolicum),又名蒙古韭。种子来源于中国科学院沙漠研究所宁夏沙坡头沙漠试验站。2材料类别幼苗的叶片。3培养条件(1)愈伤组织诱导培养基:MS+2,4-D2.0mg·L-1(单位下同)+KT0.2+CH(水解酪蛋白)500+蔗糖3%;(2)分化培养基同(1),但2,4-D为0.5;(3)生根培养基:1/2MS+1BA1.0+蔗糖3%。上述培养基均加0.7%琼脂粉固化,pH调至5.8~6.2。培养温度(25±2)℃。愈伤组织诱导阶段为暗培养,分化阶段每日光照12… 相似文献
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扁蓿豆体细胞胚的诱导和植株再生 总被引:2,自引:0,他引:2
扁蓿豆实生苗的根、下胚轴、子叶、叶片和叶柄外植体,在含2,4—D2—0.25mgL-1与KT0.25-2mgL-1及2,4—D0.5mgL-1与ZT0.5mgL-1或BAP0.5mgL-1与NAA0.05mgL-1的MS琼脂培养基上均可产生愈伤组织.愈伤组织在含2,4—D0.5—0.1mgL-1与KT0.5—0.1mgL-1或BAP0.25+NAA0.05mgL-1的MS培养基上可诱导分化出体细胞胚.体细胞胚在无激素的培养基上发育成完整植株.用海藻酸钠包襄体细胞胚制成人工种子,其发芽率和植株转换率分别为95%和53%. 相似文献
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酸枣的组织培养和植株再生 总被引:8,自引:0,他引:8
1植物名称酸枣(Zizyphus jujubavar.Spinosa)。2材料类别沂河岸堤自然生长的野生植株上的嫩芽。3培养条件诱导愈伤组织培养基:(1)MS+6-BA2mg·L’(单位下同)+NAA0.1;(2)MS+6-BA2+NAA0.2;(3)MS+6.BA2+NAA0.4;(4)MS+6.BA2+NAA1;(5)MS+6.BA2+NAA0.01;(6)MS+6.BA2+NAA0.05;(7)MS+6-BAI+NAA0.5。芽分化培养基:(8)MS+6-BAI;(9)MS+6-BA2;(… 相似文献
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虎头、克4和Favorita3个马铃薯品种的根、茎、叶外植体在附加NAA和BA各1mg/L的MS培养基上诱导出愈伤组织。在附加0.2mg/LNAA和1mg/LBA的MS培养荐,愈伤组织上分化产生不定芽。1.5-2.5cm高的不定芽在MS+0.05mg/LNAA培养基上生根形成再生完整植株。3个马铃薯品种中,虎头茎的愈伤组织诱导频率最高,达98%。Favorita叶愈伤组织的不定芽分化频率和不定芽生 相似文献
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栝楼的快速繁殖及愈伤组织的诱导 总被引:15,自引:0,他引:15
本文对栝楼的快速繁殖及愈伤组织的诱导进行了初步研究。结果表明:含腋芽的栝楼外植体在MS+6-BA1.0+NAA0.2上可以快速繁殖;茎切段、叶片切块及子叶切块在MS+2,4-D1.0+6-BA0.5上能够形成愈伤组织但以茎段形成愈伤组织的速度最快,质量最好;愈伤组织在MS+6-BA1.0+NAAO.1上分化成芽。 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
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Michael Hesse 《Plant Systematics and Evolution》1980,134(3-4):229-267
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout. 相似文献
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Surveillance of Class Ⅰ Newcastle Disease Virus at Live Bird Markets and Commercial Poultry Farms in Eastern China Reveals the Epidemic Characteristics 下载免费PDF全文
Xiaolong Lu Xiaoquan Wang Tiansong Zhan Yifan Sun Xin Wang Naiqing Xu Tianxing Liao Yu Chen Min Gu Shunlin Hu Xiaowen Liu Xiufan Liu 《中国病毒学》2021,36(4):818-822
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正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various 相似文献
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Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi 《中国病毒学》2019,34(1):102-105
<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV 相似文献