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排序方式: 共有173条查询结果,搜索用时 15 毫秒
1.
Y Takashima Y Yamaga S Mitsuda 《Journal of industrial microbiology & biotechnology》1998,20(3-4):220-226
A novel thermophilic Bacillus smithii strain SC-J05-1, isolated from a hot spring, had the ability of hydrating nitrile to form amide. The nitrile hydratase was
purified to homogeneity from the microbial cells of SC-J05-1 and was characterized. The enzyme was a 130-kDa protein composed
of two different subunits (25.3 kDa and 26.8 kDa) and contained cobalt ions. This enzyme had the optimal temperature of 40°C
and was stable up to 50°C. The optimal pH was in the alkaline region higher than pH 10.
Received 02 September 1997/ Accepted in revised form 06 February 1998 相似文献
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H Mitsuda Y Ishida H Yoshikawa S Ueno 《Comparative biochemistry and physiology. A, Comparative physiology》1988,91(4):749-757
1. The effects of a high concentration of CO2 (PCO2 = 250 mmHg and PO2 = 360 mmHg in water) and MS222 (tricaine methanesulfonate, 1/8000 or 1/5000) on the electrocardiogram (ECG) in carp were examined using five kinds of bipolar leads from the body surface. 2. In the carp anesthetized with the high concentration of CO2 for 30 min, the QRS duration, PQ interval and and direction of the QRS axis on the frontal plane significantly changed. Even after recovery from anesthesia, delay in the QRS duration was still recognized. 3. The concentration of CO2 used in this study had an anesthetic action to the same degree as 1/8000 of MS222 and had a much more severe effect on the ECG of the carp than 1/5000 of MS222. 相似文献
5.
Choonkyun Jung Pingzhi Zhao Jun Sung Seo Nobutaka Mitsuda Shulin Deng Nam-Hai Chua 《The Plant cell》2015,27(7):2016-2031
MYC2 is an important regulator for jasmonic acid (JA) signaling, but little is known about its posttranslational regulation. Here, we show that the MYC2 C-terminal region interacted with the PLANT U-BOX PROTEIN10 (PUB10) armadillo repeats in vitro. MYC2 was efficiently polyubiquitinated by PUB10 with UBC8 as an E2 enzyme and the conserved C249 in PUB10 was required for activity. The inactive PUB10(C249A) mutant protein retained its ability to heterodimerize with PUB10, thus blocking PUB10 E3 activity as a dominant-negative mutant. Both MYC2 and PUB10 were nucleus localized and coimmunoprecipitation experiments confirmed their interaction in vivo. Although unstable in the wild type, MYC2 stability was enhanced in pub10, suggesting destabilization by PUB10. Moreover, MYC2 half-life was shortened or prolonged by induced expression of PUB10 or the dominant-negative PUB10(C249A) mutant, respectively. Root growth of pub10 seedlings phenocopied 35S:MYC2 seedlings and was hypersensitive to methyl jasmonate, whereas 35S:PUB10 and jin1-9 (myc2) seedlings were hyposensitive. In addition, the root phenotype conferred by MYC2 overexpression in double transgenic plants was reversed or enhanced by induced expression of PUB10 or PUB10(C249A), respectively. Similar results were obtained with three other JA-regulated genes, TAT, JR2, and PDF1.2. Collectively, our results show that MYC2 is targeted by PUB10 for degradation during JA responses. 相似文献
6.
Sunao Imai Shoichi Naito Tatsuya Takahashi Akira Yamauchi Etsuo Nakamura Masaaki Sato Yuuichi Mitsuda Hiroyuki Takagi Yoshito Numata Ikuo Fujii Shoji Yamane 《Analytical biochemistry》2015
The measurement of plasma insulin is important for clinical diagnosis of diabetes and for preclinical research of metabolic diseases, especially in rodent models used in drug discovery research for type 2 diabetes. Fasting immunoreactive insulin (F-IRI) concentrations are used to calculate the homeostasis model assessment ratio (HOMA-R), an index of insulin sensitivity. However, even the most sensitive commercially available enzyme-linked immunosorbent assay (ELISA) kits cannot measure the very low F-IRI concentrations in normal rats and mice. Therefore, we sought to develop a new rodent insulin ELISA with greater sensitivity for low F-IRI concentrations. Despite repeated efforts, high-affinity antibodies could not be generated by immunizing mice with mouse insulin (self-antigen). Therefore, we generated two weak monoclonal antibodies (13G4 and 26B2) that were affinity maturated and used to develop a highly sensitive ELISA. The measurement range of the sandwich ELISA with the affinity maturated antibodies (13G4m1 and 26B2m1) was 1.5 to 30,000 pg/ml, and its detection limit was at least 10 times lower than those of commercially available kits. In conclusion, we describe the development of a new ultrasensitive ELISA suitable for measuring very low plasma insulin concentrations in rodents. This ELISA might be very useful in drug discovery research in diabetes. 相似文献
7.
Fine structure of protein bodies isolated from rice endosperm 总被引:1,自引:0,他引:1
H Mitsuda K Murakami T Kusano K Yasumoto 《Archives of biochemistry and biophysics》1969,130(1):678-680
8.
A review of spatial-explicit factors determining spatial distribution of land use/land-use change 总被引:2,自引:0,他引:2
Land development is necessary for human progress, but its impact has resulted in the degradation of ecosystem services not
only locally and regionally, but globally as well. Human behavior toward land use/land-use change (LULUC) must be examined
and fully understood in order to achieve better land management. Several studies were recently conducted on LULUC patterns,
suggesting a relationship between spatial distribution of LULUC and land attributes. We reviewed these studies and listed
the factors determining spatial distribution of LULUC, and then we categorized them into: (1) socioeconomic factors, subcategorized
into accessibility, local community development, spatial configuration, and political restrictions; and (2) natural environmental
factors, subcategorized into topography and productivity. Here, we discuss the effects of these factors, especially road construction
as a socioeconomic, accessibility factor, and slope as a natural environmental, topography factor. We also discuss the future
work required to provide the tools for better land management. 相似文献
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Katsumi Watanabe Yasuyuki Yamada Saburo Ueno Hisateru Mitsuda 《Bioscience, biotechnology, and biochemistry》2013,77(6):1727-1731
Cryostorage is one suitable method to preserve various desired types of cells. However, all cells do not survive after storage in liquid nitrogen. This suggests the possibility that the properties of the cells which survive after the storage differ from those of the unfrozen original cells.Therefore, we did the same freeze-thaw procedure of cultured green Lavandula vera cells over again and compared the metabolic and the differentiation potentials of the cells which survived after the repeated freeze-thaw procedures with those of the unfrozen original cells. The results we found were that the frequency of colony formation of the cells which survived after the procedures was high, but that the biosynthetic capability for biotin and the differentiation potentials such as chloroplast formation and plantlet formation of the cells were equal to those of the unfrozen original cells. Cryostorage of cells in liquid nitrogen is discussed in terms of the preservation of various desired types of cells. 相似文献