Nitrile hydratase from a thermophilic Bacillus smithii |
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| Authors: | Y Takashima Y Yamaga S Mitsuda |
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| Institution: | (1) Biotechnology Laboratory, Sumitomo Chemical Co, 4-2-1, Takatsukasa, Takarazuka, Hyogo, 665, Japan, JP |
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| Abstract: | A novel thermophilic Bacillus smithii strain SC-J05-1, isolated from a hot spring, had the ability of hydrating nitrile to form amide. The nitrile hydratase was
purified to homogeneity from the microbial cells of SC-J05-1 and was characterized. The enzyme was a 130-kDa protein composed
of two different subunits (25.3 kDa and 26.8 kDa) and contained cobalt ions. This enzyme had the optimal temperature of 40°C
and was stable up to 50°C. The optimal pH was in the alkaline region higher than pH 10.
Received 02 September 1997/ Accepted in revised form 06 February 1998 |
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| Keywords: | : nitrile hydratase thermophile Bacillus smithii |
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