猪圆环病毒2型及猪繁殖与呼吸综合症病毒的快速检测

According to the published genome sequences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV),primers were designed and PCR,RT-PCR were set up for the detection of PCV2 and PRRSV,respectively,With the established methods,38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2,27 samples were positive for PRRSV and among the above positive samples,18 samples were positive for both viruses,The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratiory diseases.     

农药醚菊酯类似物构效关系的人工神经网络方法研究

本文以醚菊酯类似物作为研究对象,尝试使用神经网络方法进行构效关系分析,并对该种农药活性进行了预测。在所研究的样本集中,由结构预测活性的成功率可达100％,本文的研究表明：神经网络方法以其极强的非线性能力,可望成为农药构效关系研究的一种有效的工具．     

S.aureus对PMN粘弹性和细胞骨架影响的研究

利用微管吸吮技术和免疫组化染色,研究了在不同浓度金黄色葡萄球菌及其代谢液作用下多形核中性粒白细胞粘弹性的变化,以及在金黄色葡萄球菌作用下PMN细胞骨架的改变。结果发现金黄色葡萄球菌代谢液对PMN粘弹性无显著影响,而金黄色葡萄球菌悬液则对PMN的粘弹性有显著影响。各参数值随金黄色葡萄球菌浓度的增加而显著递增,至金黄色葡萄球菌浓度达10倍PMN浓度时趋于稳定;微丝和微管的免疫组化染色发现其细胞骨架形态发生改变,定量分析其光密度有显著增加。这些结果表明：PMN受金黄色葡萄球菌刺激后,细胞骨架发生了重新组装和骨架蛋白的表达,使PMN刚性和粘性均增加,促进在局部微循环中滞留粘附,发生炎性反应。