多西紫杉醇修饰的人工晶体与眼组织相容性分析

目的：探讨经多西紫杉醇修饰的人工晶体对眼组织相容性的影响。方法：按照随机数字表法将32 只日本大耳兔分为两组： 实验组通过手术植入表面经多西紫杉醇修饰处理后的疏水性人工晶体，对照组植入疏水性人工晶体。比较两组人工晶体亲水角、 术后24 小时光耀斑块计数以及人工晶体周围组织炎症浸润数。结果：实验组的亲水角小于对照组，差异有统计学意义（P<0.05）。 实验组光耀斑块计数低于对照组，差异有统计学意义（P<0.05）。实验组家兔人工晶体周围组织炎症浸润计数低于对照组，差异有 统计学意义（P<0.05）。结论：人工晶体表面经多西紫杉醇修饰后，其亲水性、与眼组织的组织相容性增加，且可缓解光耀斑炎症感 染和降低并发症的发生，有重要的临床参考价值。     

转mCry1Ac基因玉米BT799对斑马鱼的生态毒理学效应

转基因作物的饲用安全性问题一直是人们关注的焦点之一.为评估转mCry1Ac基因玉米BT799对鱼类的生态毒理效应,本研究以斑马鱼为受试动物,设置5个处理:含20%转基因玉米膨化饲料组(GMF)、含20%亲本玉米膨化饲料组(PF)、转基因玉米粉(GMM)、亲本玉米粉(PMM)以及商业饲料对照组(CF),通过98 d的喂养试验,调查斑马鱼的生长表现、组织病理、繁殖、肝脏中抗氧化酶活性及敏感蛋白mRNA的表达水平.结果表明:转mCry1Ac基因玉米对斑马鱼的各项生长指标、肝脏、脑和肠道的组织病理、产卵量、受精卵孵化率、肝脏中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,以及SOD、CAT、热激蛋白70(HSP70)和卵黄蛋白原(VTG)mRNA表达量均无显著影响.但在试验后期,饲料组(GMF和PF)和玉米粉组(GMM和PMM)斑马鱼的体重、体长和特定生长率显著低于商业饲料组;饲料组斑马鱼的孵化率显著低于玉米粉组和商业饲料组;饲料组(3.85±0.76)雄鱼肝脏中的VTG mRNA表达量显著高于玉米粉组(1.60±0.56).研究表明,转mCry1Ac基因玉米对斑马鱼没有明显生态毒理效应,但由于配制的膨化饲料与商业饲料在营养成分和适口性上的差异,可能导致个别指标与商业饲料组相比有显著差异.     

罗伊氏乳杆菌辅助治疗种植体周围炎的临床和微生物学疗效

目的探究罗伊氏乳杆菌辅助治疗种植体周围炎(PIS)的临床疗效,并分析治疗后种植体周围的微生物环境。方法 86例2016年12月-2018年12月于我院就诊的PIS患者,按照随机数字表法将所有患者分为常规组(n=43)和联合组(n=43)。所有患者均给予一次牙周基础治疗及种植体清洗治疗,而联合组在此基础上增加口服拜奥益生菌滴剂(主要成分为罗伊氏乳杆菌),共计治疗90 d。而后于治疗前后分别对比两组患者出血指数(BI)、菌斑指数(PLI)、种植体临床冠高度、种植体探诊深度以及以上4组数据于两个时间点间的差值。同时,取患者种植体周围口袋中分泌物,并分析其治疗前后龈沟液中细胞因子间的差异;根据以往经验,采用real-time PCR技术分析标本中常见致病菌的负荷量。结果联合组治疗后BI显著低于常规组(P0.05),两组患者治疗前后BI、PLI及种植体周围探诊深度等指标比较差异具有统计学意义(均P0.05);联合组治疗后龈沟液中IL-1β、IL-6、TNF-α和MMP-8均显著低于常规组(均P0.05);联合组治疗前后福赛斯拟杆菌及牙龈卟啉单胞菌负荷量差异具有统计学意义(均P0.05)。结论罗伊氏乳杆菌辅助治疗PIS能更有效缓解患者症状,同时调节种植体周围细胞因子及微生物环境,对PIS有较好的治疗作用。     

泰顺杜鹃扦插繁殖试验研究

本研究采用插床育苗、薄膜覆盖的方法对泰顺杜鹃插穗进行不同剂型生根粉、不同浸泡时间、不同年龄、不同插穗长度和不同品种对比的扦插实验。结果表明:(1)生长调节剂GGR 6号相对于萘乙酸、清水生根率和不定根数较多;(2)按照高浓度速蘸低浓度浸泡原则,同激素的GGR 6号在同一浓度100 mg/L进行浸泡,以1.5 h~2h效果显著;(3)对于不同年龄生的插穗,一年生、两年生和三年生的虽不存在显著差异,但一年生插穗生根性状指标还是略高于其他;(4)对于不同规格的插穗,长度6~10 cm的生根率明显好于其他规格;(5)不同品种杜鹃生根状况存在差异,实验结果刺毛杜鹃整体性状优于泰顺杜鹃。     

不稳定性骨盆骨折合并创伤休克的急救护理研究进展

<正>骨盆骨折多由车祸、压砸等创伤导致,伤情复杂,而且一些严重伤者可并发出血性休克,入院时情况危急,极易危及患者生命,需要进行系统的急救[1]。急救工作需要有序并且合理的进行,防止病情的进一步加重,因此护理难度较大,对护理人员的要求也较高。现就不稳定性骨盆骨折的急救护理进展综述如下。1不稳定性骨盆骨折合并创伤休克的急救与护理现状不稳定性骨盆骨折合并休克患者的救治目前尚无统一规范的方法 ,一些医院采用现场简单处理后迅速转运院内进     

慢病毒介导的RNAi对大鼠SOCS3基因表达的抑制作用

目的研究慢病毒表达载体介导的RNA于扰（RNAi）对大鼠下丘脑细胞中细胞因子信号转导抑制因子3（SOCSB）的抑制作用。方法根据大鼠SOCS3基因（NM053565）,用Ambion在线软件选择3个靶序列,设计并合成包含各正反义靶序列的互补单链寡核苷酸,与经BamHI和XhoI酶切后的慢病毒载体质粒pRNA-1enti-GFP连接产生pRNA-Lenti-SOCS3-GFP慢病毒重组质粒,与慢病毒包装混合物共转染293T细胞,包装产生慢病毒,收集病毒上清,采取逐孔稀释滴度法测定病毒滴度,然后转染大鼠下丘脑细胞,通过荧光显微镜观察细胞转染情况,利用荧光实时定量PCR方法检测RNAi组（siRNAl,siRNA2,siRNA3）、空白细胞组和阴性序列组（siRNA—Negtive）中SOCS3的表达情况。结果将目的序列成功连接到载体上,并经测序分析证实载体构建成功。系列稀释法检测慢病毒悬液的滴度为1．0×10^10TU／L。荧光实时定量PCR检测显示慢病毒感染大鼠下丘脑细胞后,与空白细胞组相比,3个RNAi组都有不同程度的抑制SOCS3表达,其中siRNAI组的抑制效果最好,可使SOCS3mRNA表达下调达80％。结论构建的pRNA-Lemi-SOCS3-GFP慢病毒载体可有效地抑制大鼠SOCS3的表达,为以SOCS3基因为靶点的相关疾病的基因治疗研究奠定基础。     

微流控振荡流PCR快速检测单增李斯特氏菌

一种高通量振荡流PCR微流控技术已被成功开发,借此来快速检测食品致病菌-单增李斯特氏菌(L.monocytogenes).该PCR微流控装置主要由基于LabView的温度控制与采集系统、三个铜加热块以及聚四氟乙烯(PTFE)毛细管等构成.在该微流控装置上,三个铜块维持PCR反应所需要的三个温度,而包埋在铜块沟槽中的PT...     

脐腹小蠹形态特征和生物学特性

脐腹小蠹Scolytus schevyrewi Seme.是榆树的一种重要蛀干害虫。本研究在宁夏盐池县通过室内观察和林间调查相结合,研究了脐腹小蠹的形态特征及其生活史、成虫寿命、习性、交尾行为等生物学特性。结果显示:脐腹小蠹虫在宁夏盐池县一年发生2代,以老熟幼虫或蛹越冬,老熟幼虫约占98.2%,蛹约占1.8%。越冬幼虫于5月上旬开始化蛹,5月中旬为化蛹盛期,5月下旬开始羽化,6月上旬为羽化盛期。第2代幼虫于6月底、7月初开始化蛹,7月下旬达羽化盛期,8月上旬羽化结束;脐腹小蠹雌成虫平均寿命为(27.14±3.74)d,雄成虫的平均寿命为(16.86±4.13)d,差异显著(P<0.05);此外,脐腹小蠹无滞育现象;脐腹小蠹的4种天敌,分别是榆小蠹长茧蜂Elachistocontrum sp.、虱形螨Pedieuloids ventricosus Newport、拟截斑郭公虫Thansimus sp.和蠼螋Labidura sp.。     

桂西北典型喀斯特区生态服务价值的环境响应及其空间尺度特征

Study on ecosystem service values (ESVs) is the bridge of understanding ecosystem and economic decision-making. To investigate the response of ESVs to ambient environment and their spatial scales is an urgent work in the process of ecological restoration and sustainable development in southwest China. Based on the previous research results, the remote images, and weather data of 31 years (1975-2005), the response of ESVs to ambient environment and their spatial scales in a typical karst area of northwest Guangxi, China were evaluated with the method of empirical mode decomposition (EMD) in this paper. The results showed that ESVs had obvious variations with changes in elevation, rocky types and soil types, and had corresponding characteristics of spatial scales. The ESVs in middle and low elevation areas were higher, but they decreased due to human activities such as deforestation. The ESVs in peak-cluster depression areas were lower, and they would increase as influenced by policies such as returning farmland to forestland. The ESVs were influenced by rocky types, showing lower values but an increasing trend in the typical karst regions while higher values but a decreasing trend in non-karst regions. The average ESVs in the west part of the study region, which is not only the main nature reserve for rare wildlife species in Guangxi, but also one of the best preserved natural vegetation regions in China, were more than 15,000 RMB Yuan ha-1,. Comparatively, the ESVs were less than 10,000 Yuan ha-1 in the middle part of the region attributed to low vegetation coverage, serious peak-cluster depression and karst rocky desertification. After about 20 years, that is from 1985 to 2005, the ecosystem conditions had been improved and the ESVs had increased in the middle and eastern part of this study region. On the contrary, in most western part, which was dominated by subtropical evergreen broad-leaved forest, they had been worsened, and the balance values of ESVs were negative. As for the relationships of ESVs with rocky types and soil types, lime soil and red soil were the two main soil types that contributed to ESVs (The total contribution was above 60%, and it is 63.77%,64.37%,64.56%,64.91% respectively in the four years of 1985, 1990, 2000 and 2005.). The variance contribution of intrinsic mode function (IMF) and trend (R) showed that there were obvious special sales for ESVs in this study area, and the main spatial scales were about 2.7 km, 5.5 km, and 11.6 km. The variance contribution ratio was 12.29%, 11.26%, 11.49% respectively, and the trend (R) was high (17.74%). In conclusion, this study indicated that ecosystem conditions in the typical karst area were improved owing to the application of rocky desertification control policies, such as ecological migration and returning farmland to forestland. Besides, the main spatial scales of ESVs in the study area were the interactions of terrain, physiognomy, land use / land cover and human activities.