云南23个少数民族人群腺苷酸激酶(AK1)多态分布调查

采用淀粉凝胶电泳法,对云南彝、白、哈尼、壮、傣、苗、傈僳、回、拉祜、佤、纳西、瑶、藏、景颇、布朗、普米、怒、阿昌、德昂、基诺、布依、独龙和苦聪人等23个少数民族人群的红细胞腺苷酸激酶(AK1)的多态分布进行了调查。结果,在23个人群中均未检出AK21纯合子。但在彝、回、景颇、布朗、怒和德昂6个民族中检出了AK21杂合子,AK21基因频率在这6个民族中的分布在0.0045-0.0446之间,其中,仅回族的AK21基因达多态水平。 Abstract:The investigation on the distribution of adenylate kinase(AK1)polyorphism in 23 ethnic groups in Yunnan province of China,including the minorities of Yi,Bai,Hani,Zhuang,Dai,Miao,Lisu,Hui,Lahu,Wa,Naxi,Yao,Tibetan,Jingro,Bulang,Pumi,Nu,Achang,Deang,Jino,Buyi,Dulong and Kucong people,was carried out,using starch gel electrophoresis.The results showed that no AK21,homozygote and other variations were found.But AK21 heterozygotes were found in the minorities of Yi,Hui,Jingpo,Bulang,Nu and Deang.The ranges of AK21 gene frequencies were 0.0045-0.0446.     

云南汉族人群D17S30位点扩增片段长度多态性A

应用PCR技术和小型聚丙烯酰胺凝胶电泳银染法, 对云南汉族人群D17S30位点扩增片段长度多态性进行了分析。在被检的105名无关个体中,共检出12个等位基因,41种基因型。等位基因频率范围在0.0048-0.2190之间,杂合度为83.81%,DP值为0.9647。观察的基因型分布符合Hardy-Weinber g定律。 Abstract:A study on amplified fragment length polymorphism(Amp-FLP)at locus D17S30 in Han nationality of Yunnan was carried out by using PCR followed by a high-resolution PAGE technique and silver staining.In a sample of 105 unrelated individuals,a total 12 different alleles and 41 genotypes were detected.The heterozygosity was 83.81% and the probability of discrimination(DP) was 0.9647.The distribution of observed genotypes obeyed the Hardy-Weinberg equilibrium.     

云南苗族人红细胞EsD、PGM1和GLOI分布的调查

采用EsD-PGM1-GLOI同步电泳分型法,对云南省屏边苗族自治县138例苗族人红细胞EsD、PGM1和GLOI作了分型调查,计算出这3种红细胞同功酶的基因频率是:EsD1 0.6014、PGM11 0.6304(PGM61 0.0109),GLO1 0.1123;根据这3种同功酶的表型频率计算出DP值分别为EsD 0.6206 ,PGM1 0.6315,GLOI 0.3377,累积DP值0.9074。     

云南苗族EAP、ADA和AK1的分布及其基因频率

DistributionandGeneFrequcnciesofEAP,ADAandAK1intheMiaoEthnicGroupofYunnan,ChinaouJiongwenShenBinZhouLangpingZhaoLipingZhaoYonghe(DepartmentofForensicMedicine,KunmingMedicalCollege,Kunming650031)我们对居住于云南的苗族人红细胞EAP、ADA和AK1的分布进行了调查。138例苗族人血样采自云南省屏边苗族自治县民族小学的学生,各取措梢血少许,涂于干净纱布上自然晾干,制成血痕样品,低温干燥保存。按文献[3]所述方法对样品进行EAP－AK1同步电泳分型检测,对所得数据进行统计处理。本次调查所检测的EAP、ADA…     

荧光素标记JH12.6探针进行的人DNA指纹图分析

采用荧光素标记和鲁米诺化学发光技术,建立了ＤＮＡ指纹图的分析方法。用该方法标记ＪＨ１２．６探针获得了清晰易读、分辨率高的人ＤＮＡ指纹图。经对云南省７８名无关个体进行ＤＮＡ指纹图分析,计算出无关个体的相关机率为７．４×１０（－１１）,平均等位基因频率为０．０９。比较同位素（３２）Ｐ标记方法表明,用荧光素标记ＪＨ１２．６探针进行人ＤＮＡ指纹图分析,具有简便、快速、安全、经济的特点,可在法医学鉴定及其它领域里推广应用。