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云南汉族人群D17S30位点扩增片段长度多态性A
引用本文:欧炯文,邹浪萍,李建京,申滨,韩晓华,赵永和OU Jiong-Wen,ZOU Lang-Ping,LI Jiang-Jing,SHEN Bin,HAN Xiao-Hua,ZHAO Yong-He.云南汉族人群D17S30位点扩增片段长度多态性A[J].遗传,1997,19(2):11-14.
作者姓名:欧炯文  邹浪萍  李建京  申滨  韩晓华  赵永和OU Jiong-Wen  ZOU Lang-Ping  LI Jiang-Jing  SHEN Bin  HAN Xiao-Hua  ZHAO Yong-He
作者单位:1.昆明医学院法医学系, 昆明 650031 2.云南省高级人民法院法医处,昆明 650021 1.Department of Forensic Medicine,Kunming Medical College,Kunming 650031 2.Division of Forensic Medicine,Yunnan Provincial Higher People’s Court,Kunming 650021
基金项目:云南省应用基础研究基金
摘    要:应用PCR技术和小型聚丙烯酰胺凝胶电泳银染法, 对云南汉族人群D17S30位点扩增片段长度多态性进行了分析。在被检的105名无关个体中,共检出12个等位基因,41种基因型。等位基因频率范围在0.0048-0.2190之间,杂合度为83.81%,DP值为0.9647。观察的基因型分布符合Hardy-Weinber g定律。 Abstract:A study on amplified fragment length polymorphism(Amp-FLP)at locus D17S30 in Han nationality of Yunnan was carried out by using PCR followed by a high-resolution PAGE technique and silver staining.In a sample of 105 unrelated individuals,a total 12 different alleles and 41 genotypes were detected.The heterozygosity was 83.81% and the probability of discrimination(DP) was 0.9647.The distribution of observed genotypes obeyed the Hardy-Weinberg equilibrium.

关 键 词:PCR  Amp-FLP  基因频率  Key  words  扩增片段长度多态性  D17S30位点  回交  耐盐性  水稻  耐盐突变系  

A Stud on Amplified Fragment Length Polymorphism at Locus D17S30 in Han Nationality of Yunnan Province
OU Jiong-Wen,ZOU Lang-Ping,LI Jiang-Jing,SHEN Bin,HAN Xiao-Hua,ZHAO Yong-He.A Stud on Amplified Fragment Length Polymorphism at Locus D17S30 in Han Nationality of Yunnan Province[J].Hereditas,1997,19(2):11-14.
Authors:OU Jiong-Wen  ZOU Lang-Ping  LI Jiang-Jing  SHEN Bin  HAN Xiao-Hua  ZHAO Yong-He
Abstract:A study on amplified fragment length polymorphism (Amp-FLP) at locus D17S30 in Han nationality of Yunnan was carried out by using PCR followed by a high-resolution PAGE technique and silver staining. In a sample of 105 unrelated individuals, a total 12 different alleles and 41 genotypes were detected. The heterozygosity was 83.81% and the probability of discrimination(DP) was 0.9647. The distribution of observed genotypes obeyed the Hardy-Weinberg equilibrium.
Keywords:D17S30  Amplified fragment length polymorphism(Amp-FLP)  Polymerase chain reaction (PCR)  Gene frequency  
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