黑河林区驼鹿冬季食性研究

1987—1988年在黑龙江省黑河林区,应用粪便显微组织学分析技术,结合野外啃食调查,对驼鹿冬季食物组成、食物选择性和利用率进行了研究。结果表明,冬季驼鹿共采食31种(属)植物,其中柳、榛、桦、红松、杨和紫椴是主要的冬季食物(19.9％、18.0％、16.7％、14.9％、7.3％和6.7％)。驼鹿对杨、柳、红松和紫椴有正选择性,对榛、桦和毛赤杨有负选择性。选择性的强弱顺序为:杨>柳>红松>紫椴>榛>桦>毛赤杨。驼鹿对柳的选用率最高(32.1％),对桦的利用率最低(12.1％)。     

培养抗性植物的细胞/组织培养途径

抗性育种在作物品种改良中已日益显得重要。近10多年来,随着细胞/组织培养、遗传操作等生物技术的迅速发展,以及由于植物具有全能性,在人工培养条件下,从单细胞原生质体可以再生成一个完整的个体成功以来,人们试图利用细胞/组织培养等生物技术将野生种的抗性基因引入到作物中来,或是人工诱发抗性变异,从而筛选出抗性愈     

大兴安岭呼中林区狍冬季卧息生境的选择

大兴安岭呼中林区狍冬季卧息生境的选择关键词狍子,卧息点选择,大兴安岭ＷＩＮＴＥＲＲＥＳＴＩＮＧＳＩＴＥＳＥＬＥＣＴＩＯＮＢＹＲＯＥＤＥＥＲＡＴＨＵＺＨＯＮＧ,ＤＡＸＩＮＧＡＮＬＩＮＧＭＯＵＮＴＡＩＮＳＮＯＲＴＨＥＡＳＴＥＲＮＣＨＩＮＡ￥ＪｉａｎｇＺｈ...     

利用标记基因选配褐壳蛋鸡配套杂交亲本

应用本实验室研制的抗鸡红细胞抗原单价血清(4个位点, 14个等位基因)和DNA指纹技术,对我们组配成功的一个褐壳蛋鸡配套系统的5个亲本进行了群体遗传学分析。结果表明,由标记基因测定所提供的亲本品系遗传差异的大小, 与这些品系实际杂交效果的优劣相一致,证实了标记辅助选种方法有的效性。     

Physical mapping of three fruit ripening genes：Endopolygalacturonase,ACC oxidase and ACC synthase from apple（Malus x domestica） in an apple rootstock A106（Malus sieboldii

The apple rootstock,A106(Malus sieboldii),had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell.Karyotypes were prepared from root-tip cells with 2n=34 chromosomes,Seven out of 82 karyotypes(8.5%) showed one pari of satellites at the end of the short arm of chromosome 3.C-bands were shown on 6 pairs of chromosomes 2,4,6,8,14,and 16 near the telomeric regions of short arms.Probes for three ripening-related genes from Malus x domestica:endopolygalacturonase(EPG,0.6kb),ACC oxidase(1.2kb),and ACC synthase(2kb)were hybridized in situ to metaphase chromosomes of A106.Hybridization sites for the EPG gene were observed on the long arm of chromosome 14 in 15 out of 16 replicate spreads and proximal to the centromere of chromosomes 6 and 11.For the ACC oxidase gene,hylridization sites were observed in the telomeric region of the short arm of chromosomes 5 and 11 in 87% and 81% of 16 spreads respectively,proxiaml to the centromere of chromosome 1 in 81% of the spreads,and on the long arm of chromosome 13 in 50% of the spreads. Physical mapping of three fruit ripening genes in an apple rootstock A106.Twenty five spreads were studied for the ACC synthase gene and hybridization sites were observed in the telomeric region of the short arm of chromosome 12 in 96% of the spreads.chromosomes 9 and 10 in 76% of the spreads,and chromosome 17 in 56% of the spreads.     

Affinity chromatography—dependent selection （ACDS） of genomic DNA fragments bound specifically to bacterial synthesized Myc／Myn proteins

This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among genomic DNA.cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells.DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vector respectively.Fusion GST-Myc and GST-Myn synthesized in E.coli hosts showed affinity to CACGTG E-box DNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR.A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA.At least two genomic DNA fragments obtained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone.Significance of the work and of the technique itself as well asidentification of the DNAs are discussed.     

杏砧木的组织培养

1植物名称杏砧木一米兰博莱诺（Prunusarmeniacacv.Mirabolano）。2材料类别脱毒杏砧木萌蘖苗枝条的腋芽。材料来自山东临朐“世界实验室”果园,该果园的苗木来自意大利经鉴定的杏脱毒苗。3培养条件所用外植体按常规方法消毒后在超净台上逐层剥去外部鳞片,只留下带2～3个苞片的生长点（解剖镜下可见）,肉眼只见小绿点。将小绿点接入事先配制好的培养基上在全光照（1500～2000IX）和室温22～28C下培养。分化培养基组合为：（）MS＋IAA几回ms／L（单位下同）＋BA0．5;（2）MS＋IAAof＋BAI．0;（3）MS＋IAA0．1＋BA2．0。壮苗…     

动物的亲缘选择和亲缘识别

很多不同类群的动物能够识别亲属和非亲属,甚至远亲和近亲,并表现出行为上的差异,动物怎样完成这种高度准确性分辨是自60年代末期以来日趋活跃的研究领域。亲缘识别(Kin Recognifion)的作用主要在于使重亲行为成为可能,而且可以避免近亲繁殖。但在我国动物亲缘识别的研究几乎还是空白。